##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938449_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600975 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.705879612296684 31.0 31.0 34.0 30.0 34.0 2 31.858193768459586 31.0 31.0 34.0 30.0 34.0 3 31.879732102000915 31.0 31.0 34.0 30.0 34.0 4 35.600049918881815 37.0 35.0 37.0 33.0 37.0 5 35.511547069345646 37.0 35.0 37.0 33.0 37.0 6 35.61166271475519 37.0 35.0 37.0 33.0 37.0 7 35.53077582262157 37.0 35.0 37.0 33.0 37.0 8 35.55311285827197 37.0 35.0 37.0 33.0 37.0 9 37.022142352011315 39.0 37.0 39.0 33.0 39.0 10-11 36.96218977494904 39.0 37.0 39.0 33.0 39.0 12-13 36.84708848121802 39.0 37.0 39.0 32.0 39.0 14-15 37.62611173509713 39.5 36.5 41.0 32.0 41.0 16-17 37.62568742460169 39.0 36.5 41.0 32.0 41.0 18-19 37.82093514705271 39.5 37.0 41.0 33.0 41.0 20-21 37.90112317484088 40.0 37.0 41.0 33.0 41.0 22-23 37.86886642539207 40.0 37.0 41.0 33.0 41.0 24-25 37.74683223095803 40.0 36.5 41.0 32.0 41.0 26-27 37.46017804401181 39.5 36.0 41.0 32.0 41.0 28-29 37.24138025708224 39.0 36.0 41.0 31.5 41.0 30-31 36.83424352094514 38.5 35.0 40.0 31.0 41.0 32-33 36.403324597529014 38.0 35.0 40.0 30.5 41.0 34-35 36.09235658721245 38.0 35.0 40.0 30.0 41.0 36-37 35.90592786721577 38.0 35.0 40.0 30.0 41.0 38-39 35.63853488081867 38.0 35.0 40.0 30.0 41.0 40-41 35.32083031740089 38.0 35.0 40.0 28.5 41.0 42-43 34.961666458671324 38.0 34.0 40.0 26.5 41.0 44-45 34.43331003785515 38.0 33.5 40.0 24.5 41.0 46-47 33.694186114231044 37.5 33.0 40.0 21.5 41.0 48-49 33.04842381130663 37.0 33.0 40.0 15.5 41.0 50-51 32.63404051749241 37.0 32.5 40.0 9.5 41.0 52-53 32.07079828611839 36.0 31.0 40.0 7.0 41.0 54-55 31.79121926868838 35.5 31.0 39.0 2.0 41.0 56-57 31.502061649819044 35.0 31.0 39.0 2.0 40.5 58-59 31.10142518407588 35.0 30.5 39.0 2.0 40.0 60-61 30.712812512999708 35.0 29.5 39.0 2.0 40.0 62-63 30.223923624110817 35.0 28.5 38.0 2.0 40.0 64-65 29.699933441490913 35.0 28.0 38.0 2.0 40.0 66-67 29.225131661050792 34.0 28.0 37.0 2.0 39.5 68-69 28.750810765838843 34.0 26.5 36.5 2.0 39.0 70-71 28.219515786846376 34.0 26.0 36.0 2.0 39.0 72-73 27.672719331086984 33.0 26.0 35.5 2.0 38.5 74-75 27.098029868130954 33.0 24.5 35.0 2.0 37.5 76-77 25.832793377428345 31.0 23.0 34.0 2.0 36.0 78-79 26.458428387204126 32.5 24.0 35.0 2.0 36.0 80-81 26.263804650775825 33.0 23.5 35.0 2.0 36.0 82-83 25.84066392112817 32.5 21.0 35.0 2.0 36.0 84-85 25.32290943882857 32.0 19.5 35.0 2.0 35.0 86-87 24.72227214110404 31.5 16.5 34.5 2.0 35.0 88-89 24.00060734639544 31.0 7.5 34.0 2.0 35.0 90-91 23.484857939182163 31.0 2.0 34.0 2.0 35.0 92-93 22.802809601064936 30.5 2.0 34.0 2.0 35.0 94-95 22.134317567286494 30.0 2.0 34.0 2.0 35.0 96-97 21.255930779150546 29.0 2.0 34.0 2.0 35.0 98-99 20.24307084321311 29.0 2.0 34.0 2.0 35.0 100-101 17.506118390948043 22.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19801233165065213 1101 2 0.31802341088637576 1101 3 0.169165220639794 1101 4 0.2614884963182007 1101 5 0.19127345488044512 1101 6 0.13097162978137078 1101 7 0.19911096723787836 1101 8 0.14016542452415592 1101 9 0.27178215543377604 1101 10-11 0.2843155874490222 1101 12-13 0.2812135119163557 1101 14-15 0.298552765878199 1101 16-17 0.43138763630521737 1101 18-19 0.5123253929454563 1101 20-21 0.3716750948866334 1101 22-23 0.48530645373497094 1101 24-25 0.5314937583153281 1101 26-27 0.24533221077756906 1101 28-29 0.2329977808985717 1101 30-31 0.1758150564418557 1101 32-33 0.289703621460248 1101 34-35 0.2599513689330877 1101 36-37 0.5682773933188798 1101 38-39 0.6562631541734376 1101 40-41 0.9018816543890509 1101 42-43 0.8169431889442791 1101 44-45 1.0781169916701288 1101 46-47 1.0531970879151444 1101 48-49 1.0659469002057804 1101 50-51 1.1127806070320148 1101 52-53 1.146551688585241 1101 54-55 1.1522945640259508 1101 56-57 1.1873069199216815 1101 58-59 0.9958967337505946 1101 60-61 0.9950430474300127 1101 62-63 1.116838767535004 1101 64-65 1.293495049328623 1101 66-67 1.4125158721160425 1101 68-69 1.6032508558628251 1101 70-71 1.6446204153821178 1101 72-73 1.3817450327873324 1101 74-75 1.4054752428135764 1101 76-77 1.4039870991220518 1101 78-79 1.8387014613739403 1101 80-81 2.152624492287053 1101 82-83 2.3649294298738894 1101 84-85 2.380808330025719 1101 86-87 2.08087026379976 1101 88-89 2.1553973286767842 1101 90-91 2.7162627117516784 1101 92-93 2.8564395204324633 1101 94-95 3.16188525502465 1101 96-97 3.3383990982939373 1101 98-99 3.1915092021510176 1101 100-101 3.3985364492149444 1102 1 0.2507635918235529 1102 2 0.253648991692323 1102 3 0.41797544845652723 1102 4 0.168313142202571 1102 5 0.16425365819772253 1102 6 0.11506106685720852 1102 7 0.3985702897013468 1102 8 0.3565772405811458 1102 9 0.31181200533944065 1102 10-11 0.4087875820422582 1102 12-13 0.40522086175161576 1102 14-15 0.4737555199544019 1102 16-17 0.43384462146474334 1102 18-19 0.5362686781246921 1102 20-21 0.4820528651549978 1102 22-23 0.4698605355687704 1102 24-25 0.3890343795312887 1102 26-27 0.38304658097271727 1102 28-29 0.4557562219450091 1102 30-31 0.4106296209429914 1102 32-33 0.7186340937082889 1102 34-35 0.6143521179967593 1102 36-37 0.7460955341766464 1102 38-39 0.7430778031554439 1102 40-41 0.5787634656588025 1102 42-43 0.7333106525471038 1102 44-45 0.7735775531862785 1102 46-47 0.8938168958619741 1102 48-49 1.3172070731099552 1102 50-51 1.034780128307112 1102 52-53 1.0377473744290668 1102 54-55 0.9078034204366361 1102 56-57 1.1684888072242074 1102 58-59 1.2068560139736242 1102 60-61 1.336673105721811 1102 62-63 1.7913058340777646 1102 64-65 1.5067669932333274 1102 66-67 1.6412513343671726 1102 68-69 1.4940980581381744 1102 70-71 1.7993999203242517 1102 72-73 1.1604685269159134 1102 74-75 1.2873815535341961 1102 76-77 1.2605491503174555 1102 78-79 1.5114683505068847 1102 80-81 1.776293918661981 1102 82-83 1.8078344452212463 1102 84-85 2.105608391978354 1102 86-87 2.0505627534508513 1102 88-89 1.87287923569151 1102 90-91 1.959687284419303 1102 92-93 1.9614492357319477 1102 94-95 2.1305710143668257 1102 96-97 2.360276122314662 1102 98-99 2.402014397724219 1102 100-101 2.531481170568888 1103 1 0.12607705868562746 1103 2 0.3004882686830399 1103 3 0.19714822162050538 1103 4 0.2636689474460212 1103 5 0.03698774059473209 1103 6 0.022851329029492717 1103 7 -0.08264123936558576 1103 8 0.15951161609002185 1103 9 0.3788825150284154 1103 10-11 0.2905120568834789 1103 12-13 0.3290020048879114 1103 14-15 0.4920359303110189 1103 16-17 0.4191418043339894 1103 18-19 0.37605210101998665 1103 20-21 0.2516701913233774 1103 22-23 0.47472456422860176 1103 24-25 0.22310049254220132 1103 26-27 0.21246035723066115 1103 28-29 0.045153714210115936 1103 30-31 0.21695595215940244 1103 32-33 0.24066145081624768 1103 34-35 0.28582093415047893 1103 36-37 0.5683296979936117 1103 38-39 0.3696874758471935 1103 40-41 0.623238306889867 1103 42-43 0.665316840464385 1103 44-45 0.8960869164821545 1103 46-47 0.6799901575457383 1103 48-49 0.4780652395323557 1103 50-51 0.4762180048744469 1103 52-53 0.6744954610599052 1103 54-55 0.5909853126366045 1103 56-57 0.6024360470874228 1103 58-59 0.6768071619951179 1103 60-61 0.7641816548180493 1103 62-63 1.0038933605392515 1103 64-65 1.196234506667622 1103 66-67 1.1801016844730192 1103 68-69 1.2468451677294503 1103 70-71 1.2042264958005582 1103 72-73 1.0617875303355504 1103 74-75 1.159677596523938 1103 76-77 0.8799678117732448 1103 78-79 1.1326782511745321 1103 80-81 1.396709160479272 1103 82-83 1.511872875444336 1103 84-85 1.6576422626834493 1103 86-87 1.5865910875983857 1103 88-89 1.619153458130853 1103 90-91 1.8597540487899273 1103 92-93 1.6127291575687828 1103 94-95 1.5401134341681626 1103 96-97 1.9456514683495136 1103 98-99 2.069874681065695 1103 100-101 2.578255311588265 1104 1 0.12650960399102118 1104 2 0.2981495409628536 1104 3 0.2833142295256401 1104 4 0.2104270949588809 1104 5 0.3517733323614536 1104 6 0.31424069438111957 1104 7 0.2626402879668035 1104 8 0.3209196256358524 1104 9 0.35940902707256583 1104 10-11 0.3442386584197621 1104 12-13 0.4096150259931477 1104 14-15 0.5592489961623102 1104 16-17 0.6452928968837668 1104 18-19 0.4424895683926451 1104 20-21 0.3952841819266979 1104 22-23 0.3659632844911087 1104 24-25 0.5844622644864188 1104 26-27 0.5099232552841997 1104 28-29 0.37931286105178685 1104 30-31 0.33056621392389474 1104 32-33 0.5083935710340057 1104 34-35 0.7288621751080484 1104 36-37 0.7352578805575547 1104 38-39 0.6445222882865949 1104 40-41 0.7262377848524366 1104 42-43 0.5899719228717188 1104 44-45 0.8702893178543718 1104 46-47 0.7907098234417802 1104 48-49 0.36124668483029154 1104 50-51 0.4787625644985738 1104 52-53 0.5644962068276769 1104 54-55 0.7243599117864292 1104 56-57 0.9669017044817139 1104 58-59 1.112762117621937 1104 60-61 0.9615550607394461 1104 62-63 0.6326501656701318 1104 64-65 0.7885620478712916 1104 66-67 0.9767367803041758 1104 68-69 0.8938449439991167 1104 70-71 0.7147448043992561 1104 72-73 0.8875388913617286 1104 74-75 1.0562783870377679 1104 76-77 0.8256087991697711 1104 78-79 1.2247507398015713 1104 80-81 1.1166151937405182 1104 82-83 1.3366920267606197 1104 84-85 1.4971933104871624 1104 86-87 1.4926243488408346 1104 88-89 1.7963941173895606 1104 90-91 1.9940712983983993 1104 92-93 1.7958345345543911 1104 94-95 1.9905198776016775 1104 96-97 2.09380121851731 1104 98-99 2.3385061972115686 1104 100-101 2.519991458951978 1105 1 0.3496004728579436 1105 2 0.29085750617484507 1105 3 0.4499047579252142 1105 4 0.2513406392383146 1105 5 0.31618372049423016 1105 6 0.28234881646667986 1105 7 0.42124922746130267 1105 8 0.4020198092296354 1105 9 0.5878927837633938 1105 10-11 0.40108521251672613 1105 12-13 0.40415749247075894 1105 14-15 0.4240030120967617 1105 16-17 0.29301406418396425 1105 18-19 0.38717583563509095 1105 20-21 0.596727361230883 1105 22-23 0.6807605412149798 1105 24-25 0.39391834994515307 1105 26-27 0.5452198066516729 1105 28-29 0.5813608367794814 1105 30-31 0.5760776475930953 1105 32-33 0.4845752225735822 1105 34-35 0.5223048027689288 1105 36-37 0.5976473004988918 1105 38-39 0.5585550018831711 1105 40-41 0.4693587642491295 1105 42-43 0.5373072271805697 1105 44-45 0.26231807226105275 1105 46-47 0.5326054077096032 1105 48-49 0.7072706870598466 1105 50-51 1.0245087338818024 1105 52-53 0.869250395150651 1105 54-55 0.9296918889966719 1105 56-57 0.9999730072505599 1105 58-59 1.1352573841202336 1105 60-61 1.0871530334503454 1105 62-63 0.8528118661105886 1105 64-65 0.8367690635098057 1105 66-67 0.9987033886460601 1105 68-69 0.9272093072677201 1105 70-71 1.047381400760134 1105 72-73 1.0429615162069368 1105 74-75 1.3350967211324836 1105 76-77 1.400862138479237 1105 78-79 1.707829369082912 1105 80-81 1.541708068060661 1105 82-83 1.5267661825596228 1105 84-85 1.7546024258179607 1105 86-87 1.8535053691510726 1105 88-89 2.0651938047916936 1105 90-91 1.9547609674411532 1105 92-93 2.3123752139375817 1105 94-95 2.956761893857916 1105 96-97 3.339531010990253 1105 98-99 3.591957559224607 1105 100-101 3.6778591277706276 1106 1 0.10961691972710241 1106 2 0.031206698594939297 1106 3 0.06360477917368712 1106 4 0.11019283541966018 1106 5 -0.10620830553376948 1106 6 -0.16410089502322478 1106 7 0.02806143236280434 1106 8 -0.09188298315071819 1106 9 -0.01224132996836147 1106 10-11 0.036460054877899495 1106 12-13 -0.0013282006847532557 1106 14-15 -0.43178471290937637 1106 16-17 -0.19307508557361075 1106 18-19 -0.11252751362079749 1106 20-21 0.19632888339414478 1106 22-23 -0.014783350677213036 1106 24-25 -0.24445197504607563 1106 26-27 -0.30371183673825186 1106 28-29 -0.07880158985119579 1106 30-31 -0.13569786920009363 1106 32-33 -0.37325732291056113 1106 34-35 -0.4527751549548924 1106 36-37 -0.4814618642154045 1106 38-39 -0.3520344638419459 1106 40-41 -0.14003223246977115 1106 42-43 -0.21722738989161172 1106 44-45 -0.4806181906018665 1106 46-47 -1.1005728393742515 1106 48-49 -0.6181800151055654 1106 50-51 -0.6606289258611753 1106 52-53 -0.5883023327944024 1106 54-55 -0.4353251235785649 1106 56-57 -0.5574751340600592 1106 58-59 -0.20515831325736755 1106 60-61 -0.3679850954147419 1106 62-63 -0.015446228315415311 1106 64-65 -0.5520227814922514 1106 66-67 -0.4010300396155131 1106 68-69 -0.5442528911488544 1106 70-71 -0.2660721934050905 1106 72-73 -0.16283821280605792 1106 74-75 -0.08105874041467942 1106 76-77 -0.16577389727338598 1106 78-79 -0.04489498856474228 1106 80-81 -0.004739159841623319 1106 82-83 0.48218302514708355 1106 84-85 0.33776259790298724 1106 86-87 0.7313514915809094 1106 88-89 0.8528029101758356 1106 90-91 0.5347587046168734 1106 92-93 0.28324273109503295 1106 94-95 0.752383328874842 1106 96-97 0.997521678219865 1106 98-99 1.26995490454539 1106 100-101 2.2876930309493453 1107 1 0.3262125049811715 1107 2 0.23753012158836384 1107 3 0.32423748072926983 1107 4 0.22946333996938506 1107 5 0.29318711754176974 1107 6 0.17257095519771326 1107 7 0.2584883827392517 1107 8 0.22897389429671478 1107 9 0.020499743797209646 1107 10-11 0.24185839306118595 1107 12-13 0.24008557525226593 1107 14-15 0.5200557054701704 1107 16-17 0.6777453530269355 1107 18-19 0.3218492024692594 1107 20-21 0.3160600057619476 1107 22-23 0.39785066472975217 1107 24-25 0.5548084703290073 1107 26-27 0.6209880559980903 1107 28-29 0.4447040325089091 1107 30-31 0.534855180115521 1107 32-33 0.6630493690066857 1107 34-35 0.6606523035125278 1107 36-37 0.5986805038990681 1107 38-39 0.5483912547988368 1107 40-41 0.7399171637231419 1107 42-43 1.035342575260536 1107 44-45 0.7692021812796668 1107 46-47 0.807875840872299 1107 48-49 0.8829426606348889 1107 50-51 0.887898893402145 1107 52-53 1.2071705253968972 1107 54-55 0.8131903783280592 1107 56-57 0.7360795524375447 1107 58-59 0.7394700361655104 1107 60-61 0.9820754649223424 1107 62-63 1.2875517649818882 1107 64-65 1.166231797741709 1107 66-67 1.3509279068758353 1107 68-69 1.4781454521666682 1107 70-71 1.5096890448998401 1107 72-73 1.962911734588566 1107 74-75 1.5239929154999636 1107 76-77 1.53362486175293 1107 78-79 1.6972116386863831 1107 80-81 2.1053831412458983 1107 82-83 2.320724290858127 1107 84-85 2.4211164601641464 1107 86-87 2.6445282405172534 1107 88-89 2.9800758474035085 1107 90-91 2.818879065720715 1107 92-93 2.979703287326334 1107 94-95 2.885095148918264 1107 96-97 3.0806548545069 1107 98-99 3.078639410850336 1107 100-101 3.8239047765753966 1108 1 0.07174608463776977 1108 2 -0.003279847258987445 1108 3 -0.008581471672385987 1108 4 -0.07214500604235496 1108 5 0.027375337493957375 1108 6 -0.15513316709454017 1108 7 0.08420742932332814 1108 8 -0.1019106960097389 1108 9 -0.2293952065759015 1108 10-11 -0.044452553834410935 1108 12-13 -0.06812640765000566 1108 14-15 -0.046730500427102584 1108 16-17 -0.16647329509304143 1108 18-19 -0.15101901458500322 1108 20-21 -0.029940863614271507 1108 22-23 -0.08565292144717063 1108 24-25 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2316 52-53 -0.7064729183867371 2316 54-55 -0.25865895613809897 2316 56-57 0.059082950908241116 2316 58-59 -0.1840360527611402 2316 60-61 -0.08594350987233312 2316 62-63 -0.05424234433558439 2316 64-65 0.17725558703653022 2316 66-67 0.07049035377473345 2316 68-69 0.5299084088361674 2316 70-71 0.3313542955370501 2316 72-73 0.20622099542119088 2316 74-75 0.4850662658256475 2316 76-77 0.324236586476804 2316 78-79 0.635642678052001 2316 80-81 0.7685734556044359 2316 82-83 0.9160230730727967 2316 84-85 0.6153036592578864 2316 86-87 0.6622498491267095 2316 88-89 0.910945289487902 2316 90-91 0.9485024018861061 2316 92-93 0.8963062326675981 2316 94-95 1.441892090294644 2316 96-97 1.814412996676257 2316 98-99 2.2206388444385574 2316 100-101 2.4720497779914297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 20.0 5 81.0 6 148.0 7 345.0 8 606.0 9 966.0 10 1610.0 11 2480.0 12 3455.0 13 5047.0 14 6047.0 15 8039.0 16 10329.0 17 13536.0 18 11717.0 19 7125.0 20 6403.0 21 6738.0 22 7185.0 23 8182.0 24 9196.0 25 10502.0 26 12122.0 27 14106.0 28 16817.0 29 19506.0 30 23191.0 31 26900.0 32 31361.0 33 38497.0 34 48653.0 35 67207.0 36 69737.0 37 72368.0 38 38588.0 39 2156.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.953242647364696 18.942218894296765 14.78314405757311 18.321394400765424 2 15.675033209816123 23.47609392634912 38.953699357111226 21.89517350672353 3 20.58305253962311 30.125213195224426 30.22871167685844 19.063022588294025 4 12.079537418361829 17.918216231956404 35.97936686218229 34.02287948749948 5 11.797253137106633 41.52756356659127 30.943846873886148 15.731336422415948 6 27.571862390282458 36.65343816298515 17.938350180955947 17.836349265776448 7 25.894421564956943 37.93169433004701 18.855193643662382 17.318690461333667 8 27.419110611922292 32.504014310079455 19.32309996256084 20.753775115437413 9 29.683431091143557 16.56375057198719 19.532592869919714 34.220225466949536 10-11 24.82124880402679 28.564166562668998 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36-37 19.872203941210483 27.05291139556458 34.25034216229933 18.824542500925606 38-39 20.23527414813385 26.49663180877194 34.59144040098783 18.676653642106384 40-41 18.98897635436748 27.493632844123873 34.739834807562545 18.777555993946105 42-43 18.71124859804375 28.1685875269991 33.8897338529585 19.23043002199865 44-45 18.906468140195454 28.559673589467536 32.730326926372925 19.803531343964085 46-47 19.052721870894686 28.456698245932234 31.594518569423496 20.896061313749584 48-49 19.666874662007572 28.25874620408503 31.39132243437747 20.68305669952993 50-51 20.120221307042723 28.99380173884105 30.940804525978617 19.94517242813761 52-53 19.452889055285162 29.281001705561792 30.96318482465993 20.302924414493116 54-55 18.44511002953534 29.091393152793376 31.184741461791255 21.278755355880026 56-57 19.4939057365115 29.762386122550854 29.727026914597115 21.01668122634053 58-59 19.150879820292026 28.73738508257415 30.422064145763134 21.68967095137069 60-61 19.36844294687799 28.57256957444153 30.851116934980656 21.207870543699823 62-63 20.64495195307625 28.36074711926453 30.38595615458214 20.608344773077082 64-65 19.923457714547194 28.53671117766962 31.048213320021635 20.491617787761555 66-67 19.1481148071544 28.815271235147005 30.948478550617082 21.088135407081516 68-69 19.256042264653274 29.453388244103333 30.384708182536713 20.905861308706687 70-71 19.00237114688631 29.593743500145596 29.579267024418655 21.82461832854944 72-73 19.225258954199425 29.042139856067223 29.08898040683889 22.643620782894462 74-75 19.199217937518203 29.174258496609678 29.387162527559386 22.23936103831274 76-77 19.160614002246348 28.318981654810933 30.70626897957486 21.81413536336786 78-79 18.802030034527224 28.34460668081035 30.87316444111652 21.980198843545903 80-81 18.306834726902117 29.63168185032655 30.207496152086193 21.85398727068514 82-83 18.19665025155145 29.014319338470024 29.554029690309257 23.23500071966927 84-85 18.426180460336266 28.01920952089352 29.60286345664878 23.951746562121436 86-87 18.84244988289716 27.935121283596864 29.846705520681233 23.375723312824736 88-89 19.27120035875066 27.32079314513345 29.967993650312952 23.44001284580294 90-91 18.428487991893093 27.508490454582518 30.52129144764539 23.541730105879004 92-93 17.825827594727556 28.132355253601034 30.17187945891125 23.869937692760164 94-95 17.782187279004948 28.098423395315947 29.802071633595407 24.317317692083698 96-97 18.00241274595449 27.43283830442198 29.267107616789385 25.297641332834143 98-99 18.43005116685386 27.0915595490661 29.33832522151504 25.140064062565 100-101 18.68787127477245 26.90492037872107 29.785347022313925 24.621861324192558 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3630.0 1 2374.5 2 1646.5 3 2208.0 4 1801.0 5 1667.5 6 3180.5 7 6589.5 8 9255.5 9 8915.0 10 6435.5 11 4260.5 12 3689.0 13 3543.0 14 3334.5 15 3024.0 16 2755.0 17 2601.5 18 2547.5 19 2557.0 20 2570.0 21 2681.0 22 2833.0 23 3077.0 24 3337.0 25 3613.0 26 3961.5 27 4417.5 28 4980.0 29 5606.0 30 6159.5 31 6504.5 32 7035.5 33 7728.0 34 8610.0 35 9384.0 36 10061.0 37 10990.5 38 11819.5 39 12304.0 40 12755.0 41 13574.5 42 14620.0 43 15798.5 44 16798.5 45 17186.5 46 18144.0 47 21202.5 48 32176.5 49 33353.0 50 25569.5 51 23097.5 52 18920.5 53 16451.5 54 15243.0 55 14214.5 56 13277.5 57 12161.5 58 11178.5 59 10386.5 60 9215.0 61 7975.5 62 6801.0 63 4968.0 64 3364.0 65 2615.0 66 1974.5 67 1495.5 68 1112.0 69 756.0 70 537.0 71 430.5 72 393.0 73 376.0 74 325.0 75 238.5 76 151.5 77 96.0 78 70.5 79 48.0 80 25.0 81 9.5 82 4.5 83 2.5 84 2.0 85 3.0 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.041432671908149254 3 0.0 4 0.0 5 0.09667623445234827 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.319813636174549E-4 26-27 0.007903822954365822 28-29 0.0 30-31 0.0 32-33 5.823869545322184E-4 34-35 0.01164773909064437 36-37 0.0029119347726610924 38-39 0.009817380090685968 40-41 0.006572652772577894 42-43 0.004825491908981239 44-45 0.0023295478181288736 46-47 8.319813636174549E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.3279254544698195E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.487832272557095E-4 84-85 0.0038271142726402927 86-87 0.0012479720454261825 88-89 8.319813636174549E-5 90-91 9.983776363409458E-4 92-93 2.4959440908523645E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.319813636174549E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 600975.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.48695515948272 #Duplication Level Percentage of deduplicated Percentage of total 1 72.55637309380859 31.55255743265153 2 13.424445781476738 11.675765434799716 3 5.442944564465342 7.100912586313638 4 2.7659677780027225 4.811340669383139 5 1.5667084954440615 3.406569104467827 6 1.004329302360694 2.6205134002268435 7 0.6797892673404222 2.069337577071139 8 0.4802028220786529 1.6706046872953149 9 0.325425117115082 1.2736572728177682 >10 1.58434920278976 11.96917956279103 >50 0.0924003680441091 2.842258800244572 >100 0.06287825850799718 5.405243607515271 >500 0.005751060229389987 1.6826465494630964 >1k 0.007284676290560649 6.881310779770204 >5k 7.668080305853315E-4 2.9639722264967756 >10k+ 3.8340401529266574E-4 2.074130308692124 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12440 2.069969632680228 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9638 1.6037272765090063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8139 1.3542992636964932 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4995 0.8311493822538376 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4835 0.804525978618079 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4241 0.7056865926203253 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2854 0.47489496235284323 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2604 0.4332958941719705 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2114 0.35176172053745997 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1920 0.31948084362910273 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1903 0.3166521069928034 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1896 0.3154873330837389 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1848 0.30750031199301137 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1699 0.2827072673572112 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1574 0.26190773326677486 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1529 0.25441990099421774 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1492 0.24826323890344854 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1403 0.23345397063105786 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1230 0.20466741544989392 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1094 0.18203752235949913 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1022 0.1700569907234078 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1019 0.16955780190523734 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 857 0.14260160572403177 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 819 0.13627854736053913 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 819 0.13627854736053913 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 810 0.1347809809060277 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 777 0.1292899039061525 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 695 0.11564540954282625 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 690 0.11481342817920878 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 659 0.10965514372478058 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 634 0.1054952369066933 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 626 0.10416406672490537 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.66396272723491E-4 0.0 10-11 0.0 0.0 0.0 1.66396272723491E-4 0.0 12-13 0.0 0.0 0.0 1.66396272723491E-4 0.0 14-15 0.0 0.0 0.0 1.66396272723491E-4 0.0 16-17 0.0 0.0 0.0 1.66396272723491E-4 0.0 18-19 0.0 0.0 0.0 3.32792545446982E-4 0.0 20-21 0.0 0.0 0.0 6.65585090893964E-4 0.0 22-23 0.0 0.0 0.0 0.0015807645908731645 0.0 24-25 0.0 0.0 0.0 0.005491076999875203 0.0 26-27 0.0 0.0 0.0 0.013977286908773244 0.0 28-29 0.0 0.0 0.0 0.0332792545446982 0.0 30-31 0.0 0.0 0.0 0.08036939972544616 0.0 32-33 0.0 0.0 0.0 0.1503390324056741 0.0 34-35 0.0 0.0 0.0 0.2618245351304131 0.0 36-37 0.0 0.0 0.0 0.4276384208993719 0.0 38-39 0.0 0.0 0.0 0.6510254170306585 0.0 40-41 0.0 0.0 0.0 0.9149299055701152 0.0 42-43 0.0 0.0 0.0 1.138649694246849 0.0 44-45 0.0 0.0 0.0 1.381172261741337 0.0 46-47 0.0 0.0 0.0 1.684179874370814 0.0 48-49 0.0 0.0 0.0 2.019801156454095 0.0 50-51 0.0 0.0 0.0 2.3827946254003907 0.0 52-53 0.0 0.0 0.0 2.7139232081201383 0.0 54-55 0.0 0.0 0.0 3.0420566579308623 0.0 56-57 0.0 0.0 0.0 3.3934023877865136 0.0 58-59 0.0 0.0 0.0 3.8105578435043057 0.0 60-61 0.0 0.0 0.0 4.336702857855984 0.0 62-63 0.0 0.0 0.0 4.818336869254129 0.0 64-65 0.0 0.0 0.0 5.243229751653564 0.0 66-67 0.0 0.0 0.0 5.703315445734015 0.0 68-69 0.0 0.0 0.0 6.213985606722409 0.0 70-71 0.0 0.0 0.0 6.7800657265277255 0.0 72-73 0.0 0.0 0.0 7.357128000332793 0.0 74-75 0.0 0.0 0.0 7.919630600274554 0.0 76-77 0.0 0.0 0.0 8.4575897499896 0.0 78-79 0.0 0.0 0.0 9.044802196430801 0.0 80-81 0.0 0.0 0.0 9.69308207496152 0.0 82-83 0.0 0.0 0.0 10.360747119264529 0.0 84-85 0.0 0.0 0.0 11.10012895711136 0.0 86-87 0.0 0.0 0.0 11.856400016639627 0.0 88-89 0.0 0.0 0.0 12.559091476350929 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTCA 25 0.0016161444 37.99857 26-27 TTCGCGA 25 0.0016168052 37.995407 48-49 TATATGG 185 0.0 33.44391 2 GGTATAG 75 3.0415153E-4 31.728838 1 TGGTCCG 60 0.003921311 31.712975 5 ATCGCGT 30 0.0039517516 31.665476 24-25 GGATACG 60 0.0039518024 31.662838 8 GCGGTAT 80 4.4398167E-4 29.745785 1 ATGGGCG 70 0.008328853 27.182547 5 TCGCGAT 35 0.008396668 27.139578 50-51 GTATATG 295 0.0 25.813292 1 CATGGGG 1800 0.0 25.106104 4 GTGCTAC 95 0.0012154736 25.036556 3 CATGGGT 480 0.0 23.78473 4 ACTGGTA 100 0.0016538939 23.74713 8 AGTACTT 7105 0.0 23.22907 12-13 GTACATG 9300 0.0 23.18252 1 GAGTACT 7075 0.0 22.891226 12-13 TAAGACT 125 2.2319662E-4 22.833342 4 TACATGG 9265 0.0 22.807775 2 >>END_MODULE