##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938448_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600063 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.129508068319492 31.0 31.0 34.0 28.0 34.0 2 31.184357309149206 31.0 31.0 34.0 28.0 34.0 3 31.194789547097553 31.0 31.0 34.0 27.0 34.0 4 34.89948722050851 37.0 35.0 37.0 32.0 37.0 5 34.94811711436966 37.0 35.0 37.0 32.0 37.0 6 34.994770549092344 37.0 35.0 37.0 32.0 37.0 7 34.93324034309731 37.0 35.0 37.0 32.0 37.0 8 34.92433794451583 37.0 35.0 37.0 32.0 37.0 9 36.33183682380017 39.0 35.0 39.0 32.0 39.0 10-11 36.17321681223471 39.0 35.0 39.0 31.5 39.0 12-13 35.99719112826486 38.0 35.0 39.0 30.5 39.0 14-15 36.91149679283676 39.0 36.0 41.0 31.0 41.0 16-17 36.928080884840426 39.0 36.0 41.0 31.0 41.0 18-19 37.11233820448852 39.0 36.0 41.0 31.0 41.0 20-21 37.12781157978412 39.0 36.0 41.0 31.0 41.0 22-23 36.97736487668794 39.0 36.0 41.0 31.0 41.0 24-25 36.817919118492554 39.0 36.0 41.0 30.5 41.0 26-27 36.516148304428036 39.0 35.0 41.0 30.0 41.0 28-29 36.230512462858066 38.5 35.0 40.0 30.0 41.0 30-31 35.828640492748264 38.0 35.0 40.0 29.5 41.0 32-33 35.356584225323004 38.0 35.0 40.0 28.5 41.0 34-35 34.97861307896004 38.0 34.0 40.0 27.0 41.0 36-37 34.56903191831525 37.5 33.5 40.0 25.0 41.0 38-39 34.22228166042565 37.0 33.0 40.0 24.0 41.0 40-41 33.827709757142166 37.0 33.0 40.0 22.5 41.0 42-43 33.32450342714015 36.5 33.0 40.0 20.5 41.0 44-45 32.64138265482124 36.0 31.5 40.0 16.0 41.0 46-47 31.82667653229744 36.0 31.0 39.0 8.0 41.0 48-49 31.15150242557865 35.0 30.0 39.0 2.0 40.5 50-51 30.33145769694182 35.0 28.5 38.5 2.0 40.0 52-53 30.31721502575563 35.0 28.5 38.5 2.0 40.0 54-55 30.43297203793602 35.0 29.0 39.0 2.0 40.0 56-57 30.118863352681302 35.0 28.0 39.0 2.0 40.0 58-59 29.748226436224197 35.0 28.0 38.5 2.0 40.0 60-61 29.2572879847616 35.0 26.5 38.0 2.0 40.0 62-63 28.57374975627559 34.0 26.0 38.0 2.0 40.0 64-65 27.93213045963507 34.0 24.5 37.0 2.0 39.5 66-67 27.318215754012495 33.5 22.5 36.5 2.0 39.0 68-69 26.589821402086116 33.0 21.0 36.0 2.0 39.0 70-71 25.84212157723439 32.5 18.5 35.0 2.0 38.5 72-73 25.0013815216069 31.5 11.0 35.0 2.0 37.5 74-75 24.16353949501969 31.0 4.5 35.0 2.0 37.0 76-77 23.299686866212383 30.5 2.0 34.5 2.0 36.0 78-79 22.39172220250207 29.5 2.0 34.0 2.0 36.0 80-81 21.513830214494146 29.0 2.0 34.0 2.0 35.0 82-83 20.62622341320828 27.5 2.0 34.0 2.0 35.0 84-85 19.718456228762648 26.0 2.0 33.0 2.0 35.0 86-87 18.754848240934702 25.0 2.0 33.0 2.0 35.0 88-89 17.71508824906718 22.0 2.0 32.0 2.0 34.5 90-91 16.597763901457014 17.0 2.0 31.5 2.0 34.0 92-93 15.475460076691947 4.5 2.0 31.0 2.0 34.0 94-95 14.214378323609354 2.0 2.0 30.5 2.0 34.0 96-97 12.763489000321634 2.0 2.0 29.5 2.0 34.0 98-99 11.183437405739063 2.0 2.0 28.0 2.0 33.5 100-101 7.95155008724084 2.0 2.0 9.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7053750350516168 1101 2 0.5008325713185471 1101 3 0.43666625959277283 1101 4 0.5659957116460745 1101 5 0.15006550759797932 1101 6 0.37102519042482385 1101 7 0.2060432199283042 1101 8 0.2765942009217426 1101 9 0.22407038050071293 1101 10-11 0.5531411437061564 1101 12-13 0.40238522530577825 1101 14-15 0.501763756552279 1101 16-17 0.5500834056000627 1101 18-19 0.4764920634196059 1101 20-21 0.5059549395690723 1101 22-23 0.6606241261652599 1101 24-25 0.8899973919793567 1101 26-27 0.6603609029847277 1101 28-29 0.7778282994449484 1101 30-31 0.5680410437785781 1101 32-33 0.4050409161344959 1101 34-35 0.5618890731612467 1101 36-37 0.8301382123714092 1101 38-39 0.7199219018055416 1101 40-41 0.5344927827221753 1101 42-43 0.9041932995122863 1101 44-45 0.6285073544160795 1101 46-47 1.386136262746433 1101 48-49 0.9869766746697515 1101 50-51 0.9964799411357248 1101 52-53 1.2290272302170848 1101 54-55 1.472959988217518 1101 56-57 1.6664060714694457 1101 58-59 1.790722373910544 1101 60-61 1.7540668019487207 1101 62-63 1.5215715768752993 1101 64-65 1.573241801929452 1101 66-67 1.6668577360415036 1101 68-69 1.590685906437475 1101 70-71 1.8608832278032956 1101 72-73 1.9936674184924925 1101 74-75 1.8183795483849146 1101 76-77 1.906993424741696 1101 78-79 1.4953503747255539 1101 80-81 1.9612527771379291 1101 82-83 2.331008950623424 1101 84-85 2.3819354952007323 1101 86-87 2.5543850848522993 1101 88-89 2.2686689794121833 1101 90-91 2.2881985791538746 1101 92-93 2.4779152027019133 1101 94-95 2.4979893967572924 1101 96-97 2.87613659581411 1101 98-99 2.8312591304453 1101 100-101 2.550208849191405 1102 1 0.603059747343071 1102 2 0.6058036302221907 1102 3 0.5692709582648874 1102 4 0.4075210776658196 1102 5 0.66397766285845 1102 6 0.5986384011841039 1102 7 0.7422842822367812 1102 8 0.5691648512451977 1102 9 0.6838184227206625 1102 10-11 0.7518677354664547 1102 12-13 0.7778091272465275 1102 14-15 1.0162615434096125 1102 16-17 1.1369645768632495 1102 18-19 0.9488856623300563 1102 20-21 0.8536226276861996 1102 22-23 0.7591191891547098 1102 24-25 0.8631128158812942 1102 26-27 0.7646305659060673 1102 28-29 0.5822920379536356 1102 30-31 0.5465735599515469 1102 32-33 0.7285683250551642 1102 34-35 0.7413272754084446 1102 36-37 0.676808283872937 1102 38-39 1.0993839378967891 1102 40-41 1.157669493651703 1102 42-43 1.1643022542279837 1102 44-45 1.0170262512495825 1102 46-47 1.028836296794779 1102 48-49 0.7777461673493598 1102 50-51 0.889139117165687 1102 52-53 1.0688025111159618 1102 54-55 0.9390852861405712 1102 56-57 0.9425994660898027 1102 58-59 1.2733883596102373 1102 60-61 1.2638863457008505 1102 62-63 1.0394401502494937 1102 64-65 1.2697143930087869 1102 66-67 1.5321113962389852 1102 68-69 1.5833825356509585 1102 70-71 1.165762356165569 1102 72-73 1.489183250974616 1102 74-75 1.4045320849868865 1102 76-77 1.6780557332197326 1102 78-79 2.0300184033261672 1102 80-81 2.1802517556874648 1102 82-83 2.65109747632993 1102 84-85 2.4478667175364457 1102 86-87 2.6777967293875413 1102 88-89 2.8706165449552543 1102 90-91 2.726043318684006 1102 92-93 2.962607065146587 1102 94-95 2.808380952766827 1102 96-97 2.7398240319734573 1102 98-99 2.754234956117754 1102 100-101 1.9732016990381869 1103 1 0.5822896562742095 1103 2 0.7299288898483312 1103 3 0.7266091912094481 1103 4 0.4154424708596238 1103 5 0.3803828394042199 1103 6 0.5595746529815102 1103 7 0.6163727947787194 1103 8 0.5758016941913553 1103 9 0.8233838485075324 1103 10-11 0.7296006001413247 1103 12-13 0.5724105012679388 1103 14-15 0.6654371305641149 1103 16-17 0.8332515236598681 1103 18-19 0.9763357342265238 1103 20-21 0.7416318943422198 1103 22-23 0.933035150170852 1103 24-25 0.8942232169038178 1103 26-27 1.1387430439377013 1103 28-29 1.081697693809204 1103 30-31 0.9383454084896456 1103 32-33 1.1442657208052793 1103 34-35 0.88127455133111 1103 36-37 0.8249645388657427 1103 38-39 0.886952239542417 1103 40-41 0.9535010961441159 1103 42-43 1.1412426600436092 1103 44-45 0.760426155387627 1103 46-47 0.6923176835977856 1103 48-49 0.5939420452851287 1103 50-51 0.7210723333344049 1103 52-53 0.5515968872143802 1103 54-55 0.40775919571078845 1103 56-57 0.5501629746374945 1103 58-59 0.9431630840071072 1103 60-61 1.0303218785335737 1103 62-63 1.0026754913582927 1103 64-65 1.5418377353957489 1103 66-67 1.4020986146444763 1103 68-69 1.7172138186035077 1103 70-71 1.752515831161574 1103 72-73 1.8728611547931173 1103 74-75 1.6442732576981811 1103 76-77 1.522157027356176 1103 78-79 2.212437320086032 1103 80-81 2.1281827350771465 1103 82-83 2.0635115999918554 1103 84-85 2.3532861136061065 1103 86-87 2.727130488774023 1103 88-89 2.9373300774684346 1103 90-91 2.190989795757023 1103 92-93 2.254861418877214 1103 94-95 1.9815172365182239 1103 96-97 2.3736633034225694 1103 98-99 2.130938902435057 1103 100-101 1.981899574098101 1104 1 0.6076588581816438 1104 2 0.5656840533403873 1104 3 0.5730514273741925 1104 4 0.36294956149227886 1104 5 0.5025580706462733 1104 6 0.6232436441704579 1104 7 0.4680607654500264 1104 8 0.522158872401107 1104 9 0.5264337837508606 1104 10-11 0.4980903324310404 1104 12-13 0.9714730855479274 1104 14-15 0.7885948256417095 1104 16-17 0.7061536003187214 1104 18-19 0.5576653649208865 1104 20-21 0.4510241300624145 1104 22-23 0.47978146111546494 1104 24-25 0.6719952319475198 1104 26-27 0.7576997058723478 1104 28-29 0.8846314523335224 1104 30-31 0.7412999128566469 1104 32-33 1.0349380935665806 1104 34-35 1.0045819128452038 1104 36-37 0.6679274008436593 1104 38-39 0.9064251765906377 1104 40-41 0.7407095059159943 1104 42-43 0.8307885335473131 1104 44-45 0.5536167770812597 1104 46-47 0.6975945368683654 1104 48-49 0.7061978000337064 1104 50-51 0.8240295050240363 1104 52-53 0.8628497286539023 1104 54-55 0.7296279812195188 1104 56-57 0.8615447366708224 1104 58-59 0.9605169814100236 1104 60-61 1.022684444735038 1104 62-63 0.9979454392269567 1104 64-65 0.7338167998767879 1104 66-67 0.7620286122438884 1104 68-69 0.7192172742681606 1104 70-71 1.1325767053913864 1104 72-73 1.3171274484297761 1104 74-75 1.2058321003172559 1104 76-77 1.3225153376961991 1104 78-79 1.5857780231330025 1104 80-81 1.662192580461344 1104 82-83 1.5753041297102754 1104 84-85 2.1991222747953785 1104 86-87 2.1591182072351565 1104 88-89 2.0605322279232148 1104 90-91 1.7699188360839742 1104 92-93 1.8353404700216434 1104 94-95 1.5522901969796123 1104 96-97 1.7705460923751115 1104 98-99 1.848919917025313 1104 100-101 1.1949830879696588 1105 1 0.6668317961897472 1105 2 0.47498801134686985 1105 3 0.48075294228418386 1105 4 0.561216238226649 1105 5 0.26314244500830597 1105 6 0.45880725389027077 1105 7 0.3488032027855539 1105 8 0.37724264409911257 1105 9 0.402494738113397 1105 10-11 0.5615028930120616 1105 12-13 0.470206232628712 1105 14-15 0.6588109177500314 1105 16-17 0.6483048452184477 1105 18-19 0.6338653095485114 1105 20-21 0.6466046870861746 1105 22-23 0.7014499595451866 1105 24-25 0.6279462245952985 1105 26-27 0.6596388577800312 1105 28-29 0.8372083654072711 1105 30-31 0.905189570806364 1105 32-33 0.7549440223263844 1105 34-35 0.5726871427384523 1105 36-37 0.58476396003757 1105 38-39 1.0201482979148793 1105 40-41 1.004129617276 1105 42-43 1.074190690832836 1105 44-45 0.9492258592700935 1105 46-47 0.9776543278516172 1105 48-49 1.4478185327948516 1105 50-51 1.406037956303333 1105 52-53 1.4564807864061784 1105 54-55 1.583651847274293 1105 56-57 1.5810556699191416 1105 58-59 1.0851537749577425 1105 60-61 1.8351874161063968 1105 62-63 1.8111769209414845 1105 64-65 1.972509508341215 1105 66-67 2.2251989885803063 1105 68-69 2.588440578768708 1105 70-71 2.4493640446926292 1105 72-73 2.6179756526028406 1105 74-75 2.6315002743431357 1105 76-77 2.691851903881574 1105 78-79 2.3111310220507235 1105 80-81 2.0482149699193393 1105 82-83 2.3459715906070286 1105 84-85 2.637334529989335 1105 86-87 2.396681095436083 1105 88-89 2.588725997691572 1105 90-91 2.498574601663048 1105 92-93 2.1623115729107933 1105 94-95 1.625721522644712 1105 96-97 1.770564270176381 1105 98-99 1.6249917780686065 1105 100-101 0.9827418769365739 1106 1 0.1124536867370125 1106 2 0.11504605446461369 1106 3 -0.16588055688662706 1106 4 0.053430168574912784 1106 5 0.05079584467662812 1106 6 -0.32560402305832525 1106 7 0.1401630701155696 1106 8 -0.21795636087601622 1106 9 -0.022090668188425866 1106 10-11 0.1160344021331241 1106 12-13 0.10758185451925328 1106 14-15 0.23929184643991164 1106 16-17 -0.20545966556098705 1106 18-19 0.4266886926330855 1106 20-21 0.3778650519286302 1106 22-23 0.22889191642744322 1106 24-25 0.3400067552752333 1106 26-27 0.27546389923940495 1106 28-29 0.4508526440142333 1106 30-31 0.1530129538909435 1106 32-33 0.012962264449100758 1106 34-35 -0.004506057924892559 1106 36-37 0.030671920236571282 1106 38-39 0.15809605910890667 1106 40-41 0.19577555425776438 1106 42-43 -0.43539471546898767 1106 44-45 0.46634443306776774 1106 46-47 0.5860332664917536 1106 48-49 0.3350946521978422 1106 50-51 0.062472450499022614 1106 52-53 0.2179691777826278 1106 54-55 0.3547671043223879 1106 56-57 0.7347206782110156 1106 58-59 0.5927822990041776 1106 60-61 0.5999469517614564 1106 62-63 0.6633037866131275 1106 64-65 0.7819871202815101 1106 66-67 0.8678689719965575 1106 68-69 1.2858825356509591 1106 70-71 0.9348532652564785 1106 72-73 1.2182741600655262 1106 74-75 0.9736229940777967 1106 76-77 0.903510278674279 1106 78-79 1.2070638578716206 1106 80-81 0.9135093314450415 1106 82-83 1.29821868845114 1106 84-85 1.2891545963243267 1106 86-87 0.829160365751175 1106 88-89 0.7939498782885899 1106 90-91 1.3011948338355204 1106 92-93 1.2775313075708272 1106 94-95 1.4043658012516769 1106 96-97 1.2916422137916381 1106 98-99 1.0935531379359364 1106 100-101 0.8360047293412176 1107 1 0.14288799726904244 1107 2 0.21853682536580976 1107 3 0.30582411195159054 1107 4 0.19334873535015618 1107 5 0.2921802094974737 1107 6 0.21456970115448826 1107 7 -0.03554811338062791 1107 8 0.17654418201215094 1107 9 0.16105265428713977 1107 10-11 0.11665600908209228 1107 12-13 -0.0016608164905136391 1107 14-15 0.3166968410110371 1107 16-17 0.49784109448243896 1107 18-19 0.35121909437032883 1107 20-21 0.4100040312988753 1107 22-23 0.45274370795838337 1107 24-25 0.24678471401573177 1107 26-27 0.33257844864223074 1107 28-29 0.4859313627981692 1107 30-31 0.7121199028594489 1107 32-33 0.6382662818215294 1107 34-35 0.5064412819230952 1107 36-37 0.5668527019955292 1107 38-39 0.4308675031914291 1107 40-41 0.08536567369315406 1107 42-43 0.49751516509562066 1107 44-45 0.6406033907224895 1107 46-47 0.6767308777838252 1107 48-49 0.33977163373964814 1107 50-51 0.466044654624973 1107 52-53 0.7068481137218221 1107 54-55 0.8912736189803674 1107 56-57 1.036164760730017 1107 58-59 0.5779071632821342 1107 60-61 0.8916950516528779 1107 62-63 0.9224487377531929 1107 64-65 0.8823617131153867 1107 66-67 1.0812674519096959 1107 68-69 1.007486770178648 1107 70-71 0.960097564930745 1107 72-73 0.9458963099026612 1107 74-75 1.0112451439149304 1107 76-77 0.9793408975667859 1107 78-79 1.4763553888162448 1107 80-81 1.6074208406741448 1107 82-83 1.4144673312307248 1107 84-85 1.0843961815577678 1107 86-87 1.158427466728373 1107 88-89 1.467945535183265 1107 90-91 1.009815897896324 1107 92-93 1.1137500915013394 1107 94-95 1.4297105352364738 1107 96-97 1.29167478708154 1107 98-99 1.079929359434308 1107 100-101 0.24602373042699277 1108 1 -0.05154315370532814 1108 2 0.04631777642354251 1108 3 -0.09144137837161992 1108 4 -0.32390790409491643 1108 5 -0.0795438077720263 1108 6 -0.19922171658122778 1108 7 -0.1285415112430428 1108 8 -0.14219806703714966 1108 9 0.025737135918994625 1108 10-11 -0.14574679849879146 1108 12-13 -0.1660555862390396 1108 14-15 -0.39314101295977366 1108 16-17 -0.2504517666668278 1108 18-19 -0.3368144669245794 1108 20-21 -0.4577589607096044 1108 22-23 -0.30431898404649615 1108 24-25 -0.10011567758416362 1108 26-27 -0.3718781228774972 1108 28-29 0.029363702466056907 1108 30-31 -0.3482706164092164 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0.14912312982217202 2316 98-99 -0.2737941903083385 2316 100-101 -0.26346728083690074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2420.0 3 691.0 4 662.0 5 759.0 6 872.0 7 1100.0 8 1607.0 9 2059.0 10 2690.0 11 3895.0 12 5434.0 13 7178.0 14 8233.0 15 10276.0 16 12707.0 17 15423.0 18 13214.0 19 9929.0 20 10566.0 21 11516.0 22 13061.0 23 14431.0 24 16038.0 25 17645.0 26 19700.0 27 21735.0 28 24424.0 29 27376.0 30 30143.0 31 33356.0 32 37632.0 33 42973.0 34 49295.0 35 51100.0 36 42530.0 37 30032.0 38 7272.0 39 89.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.33628244553233 18.089524070845616 14.209612276516076 18.364581207105974 2 15.20175769073045 22.83180913452402 40.02630534875425 21.940127825991283 3 20.687643161744848 30.431310703080456 29.394377890325185 19.48666824484951 4 11.786753365845525 17.05256137718311 36.54953941061231 34.61114584635905 5 11.965267505907425 41.38683173550742 30.628229906831933 16.01967085175322 6 27.534786777272917 36.2180999624828 18.57868189586894 17.66843136437534 7 26.463527269938545 37.77879290051316 18.92340676671924 16.83427306282906 8 27.647684495170267 31.583825976199766 19.629359180829105 21.139130347800865 9 30.584206762147844 16.385563459528306 19.555914170302362 33.474315608021485 10-11 24.964510275140185 28.399615552293472 24.384837822585553 22.251036349980787 12-13 29.126227349259004 26.52999816626936 25.85618550685982 18.487588977611814 14-15 25.216957888665515 25.865497777911134 28.552174913233515 20.365369420189836 16-17 19.559062651841515 30.28896039764871 28.807028861035477 21.344948089474293 18-19 19.468356076240138 27.517528096630407 34.716466575761714 18.297649251367744 20-21 21.623680705246503 25.908523340118393 33.78736577827346 18.68043017636164 22-23 20.392346451836076 26.55727866433832 33.15506728737481 19.895307596450795 24-25 20.13936474750456 26.6542790596089 34.26807248500733 18.93828370787922 26-27 20.41916806567183 26.21204972733987 34.64242428283967 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19.430609015357255 29.464783474261658 30.900427409899343 20.204180100481743 54-55 18.47313422885904 29.339022032598916 31.081963934535516 21.105879804006534 56-57 19.49779714391188 29.92842696825837 29.718349412057837 20.855426475771914 58-59 19.293251828199264 28.75005832905587 30.35752378158935 21.599166061155515 60-61 19.559134301294616 28.40203058544325 30.911430209458292 21.12740490380385 62-63 20.86931956439855 28.09142697142324 30.4969349897833 20.542318474394914 64-65 20.027698892044317 28.445278855512445 31.19966867991947 20.327353572523766 66-67 19.409713397689348 28.61481799357551 31.098038071985968 20.877430536749173 68-69 19.480924223536235 29.195804195804197 30.674818508469603 20.648453072189966 70-71 19.157439985599545 29.286094059645222 29.906197029572603 21.65026892518263 72-73 19.425277586109193 28.741704083707504 29.52579525795258 22.307223072230723 74-75 19.322681938185017 28.8719365849756 29.998966639110375 21.806414837729008 76-77 19.549122168160064 28.047704321204208 30.693824220309168 21.70934929032656 78-79 19.23209613173114 28.015449742504288 30.975400409993163 21.777053715771405 80-81 18.861474794394294 29.240885973311464 30.294477208579064 21.60316202371518 82-83 18.71795192319712 28.745077940422764 29.532940818614033 23.004029317766083 84-85 19.056511637984194 27.69233589658977 29.804272538490512 23.446879926935527 86-87 19.53112435995184 27.545089804642092 29.957462055403482 22.966323780002583 88-89 20.033202407174517 26.747105831839473 30.35911770270011 22.86057405828589 90-91 19.38867788026768 27.096174090030527 30.481091321586305 23.034056708115482 92-93 18.98958652782037 27.555117401565354 30.288903852051362 23.166392218562915 94-95 19.033149033132364 27.498512318998813 29.755286893238676 23.713051754630147 96-97 19.30150030919604 26.896805196493666 29.112182323987447 24.689512170322846 98-99 19.804764063520846 26.37501833357778 29.043970586274483 24.776247016626886 100-101 20.82192123671949 25.563485740267218 29.485548385482062 24.129044637531234 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2879.0 1 2078.0 2 1697.5 3 2100.5 4 1697.0 5 1596.5 6 2951.5 7 5885.0 8 8528.0 9 8517.0 10 6271.5 11 4280.5 12 3705.5 13 3609.0 14 3449.0 15 3163.0 16 2948.0 17 2800.5 18 2680.5 19 2703.0 20 2707.5 21 2784.5 22 2983.5 23 3248.0 24 3504.5 25 3774.0 26 4184.0 27 4667.5 28 5140.5 29 5659.5 30 6234.0 31 6684.0 32 7050.5 33 7757.0 34 8648.0 35 9351.5 36 10122.5 37 10885.0 38 11618.5 39 12257.0 40 12760.0 41 13483.5 42 14364.0 43 15501.0 44 16339.5 45 16848.5 46 18441.5 47 21738.0 48 29472.0 49 31973.5 50 26587.5 51 22329.5 52 18801.5 53 16727.5 54 15542.0 55 14255.0 56 13057.5 57 12014.5 58 11050.0 59 10176.0 60 9321.0 61 8417.5 62 7224.5 63 5485.0 64 3879.5 65 2958.0 66 2286.5 67 1767.0 68 1387.0 69 1030.0 70 762.5 71 570.0 72 495.5 73 471.0 74 368.0 75 271.0 76 185.0 77 117.0 78 89.5 79 63.5 80 34.0 81 21.0 82 12.0 83 6.5 84 4.0 85 1.5 86 1.0 87 0.5 88 1.0 89 1.0 90 1.0 91 2.5 92 2.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3947918801859138 2 0.030830096173235143 3 0.036162869565362306 4 0.04799496052914444 5 0.06565977239056565 6 0.05632741895434313 7 0.040829046283473565 8 0.03949585293544178 9 0.03732941374489012 10-11 0.040245774193709664 12-13 0.03216328952126693 14-15 0.029496902825203355 16-17 0.022747611500792415 18-19 0.028163709477171565 20-21 0.03599622039685833 22-23 0.035079649970086474 24-25 0.04499527549607291 26-27 0.03866260709292191 28-29 0.033829781206306676 30-31 0.037662712081898066 32-33 0.03149669284725104 34-35 0.03207996493701495 36-37 0.03499632538583449 38-39 0.01616496934488545 40-41 0.012415363053546043 42-43 0.013498582648821875 44-45 0.010915520537010281 46-47 0.01974792646772089 48-49 0.017581487277169232 50-51 0.010248923862994384 52-53 0.008915730514962595 54-55 0.0071659142456708715 56-57 0.007749186335434779 58-59 0.003166334201575501 60-61 0.006499317571654975 62-63 0.010832195952758293 64-65 0.006499317571654975 66-67 0.002583062111811593 68-69 0.005832720897639081 70-71 0.01366523181732585 72-73 0.011498792626774189 74-75 0.013165284311813928 76-77 0.005582747144883121 78-79 0.008832405930710609 80-81 0.011248818874018228 82-83 0.014248503907089757 84-85 0.007499212582678818 86-87 0.0017498162692917245 88-89 0.017748136445673204 90-91 0.027747086555911626 92-93 0.011832090963782136 94-95 0.02266428691654043 96-97 0.019997900220476848 98-99 0.011832090963782136 100-101 0.12257046343467268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 600063.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.47862826690784 #Duplication Level Percentage of deduplicated Percentage of total 1 75.16167494265007 34.93411549577594 2 12.343627586625706 11.4742975612785 3 4.827497384266521 6.731263691483807 4 2.4837336315958867 4.61762128707849 5 1.4354385042879452 3.33586063204028 6 0.8814418659136477 2.4580925294814033 7 0.6113700686164633 1.9890949506916975 8 0.414213503100496 1.5401660346993302 9 0.31817720036028363 1.3309595836695969 >10 1.3715711202702496 11.041424134348983 >50 0.08318980615239854 2.681978340701786 >100 0.05581999923191949 5.033542534733128 >500 0.0046816773549351815 1.4935587934764463 >1k 0.006482322491448713 6.550534952672948 >5k 7.202580546054125E-4 2.706248859456145 >10k+ 3.6012902730270626E-4 2.0812406184115355 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12434 2.07211576117841 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8846 1.4741785445861517 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7322 1.2202052117860958 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4982 0.8302461574867972 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4784 0.7972496221230104 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3923 0.653764688041089 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2898 0.48294929032451595 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2602 0.43362113644733974 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2068 0.3446304804662177 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1990 0.33163184532290774 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1853 0.30880090923786335 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1703 0.2838035339622673 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1671 0.27847076057014014 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1611 0.2684718104599017 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1575 0.26247244039375867 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1452 0.2419745926677699 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1422 0.2369751176126507 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1391 0.23180899338902747 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1141 0.19014670126303407 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1042 0.17364843358114063 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1027 0.17114869605358102 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 963 0.16048314926932672 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 815 0.1358190723307386 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 770 0.1283198597480598 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 761 0.126820017231524 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 748 0.12465357804097237 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 733 0.12215384051341276 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 663 0.11048839871813458 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 648 0.107988661190575 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 626 0.10432237948348756 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.332458425198687E-5 0.0 12-13 0.0 0.0 0.0 1.6664916850397374E-4 0.0 14-15 0.0 0.0 0.0 1.6664916850397374E-4 0.0 16-17 0.0 0.0 0.0 4.999475055119212E-4 0.0 18-19 0.0 0.0 0.0 7.499212582678819E-4 0.0 20-21 0.0 0.0 0.0 0.0012498687637798032 0.0 22-23 0.0 0.0 0.0 0.003249658785827488 0.0 24-25 0.0 0.0 0.0 0.008582432177954648 0.0 26-27 0.0 0.0 0.0 0.019581277299216916 0.0 28-29 0.0 0.0 0.0 0.036662817070874223 0.0 30-31 0.0 0.0 0.0 0.08265798757797098 0.0 32-33 0.0 0.0 0.0 0.15581697255121546 0.0 34-35 0.0 0.0 0.0 0.2742212067732888 0.0 36-37 0.0 0.0 0.0 0.43645417231190725 0.0 38-39 0.0 0.0 0.0 0.6649301823308553 0.0 40-41 0.0 0.0 0.0 0.9174036726143755 0.0 42-43 0.0 0.0 0.0 1.1485460693293872 0.0 44-45 0.0 0.0 0.0 1.396436707479048 0.0 46-47 0.0 0.0 0.0 1.6870728573499782 0.0 48-49 0.0 0.0 0.0 2.0087890771468997 0.0 50-51 0.0 0.0 0.0 2.3591689539265044 0.0 52-53 0.0 0.0 0.0 2.68021857704941 0.0 54-55 0.0 0.0 0.0 3.008100816080978 0.0 56-57 0.0 0.0 0.0 3.3682296692180653 0.0 58-59 0.0 0.0 0.0 3.7942682684984748 0.0 60-61 0.0 0.0 0.0 4.31496359548914 0.0 62-63 0.0 0.0 0.0 4.7744153530545965 0.0 64-65 0.0 0.0 0.0 5.186288773012167 0.0 66-67 0.0 0.0 0.0 5.6335751412768325 0.0 68-69 0.0 0.0 0.0 6.1490210194596235 0.0 70-71 0.0 0.0 0.0 6.721377588686521 0.0 72-73 0.0 0.0 0.0 7.292984236655151 0.0 74-75 0.0 0.0 0.0 7.84609282691984 0.0 76-77 0.0 0.0 0.0 8.368371321011294 0.0 78-79 0.0 0.0 0.0 8.961309062548434 0.0 80-81 0.0 0.0 0.0 9.600075325424164 0.0 82-83 0.0 0.0 0.0 10.225176356482569 0.0 84-85 0.0 0.0 0.0 10.897689075980356 0.0 86-87 0.0 0.0 0.0 11.596282390349014 0.0 88-89 0.0 0.0 0.0 12.235298626977501 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAATAC 80 2.2525273E-7 41.58045 3 ATACGGC 65 0.0058366912 29.236076 6 TATCACG 70 0.008370777 27.15458 2 CGTCACT 125 7.1327013E-6 26.718433 1 GTACATG 9285 0.0 26.412176 1 TACATGG 9260 0.0 26.01825 2 GAACGCA 130 1.0003027E-5 25.579435 9 ACATGGG 9470 0.0 24.939487 3 CATGGGG 1950 0.0 24.856886 4 ATGGGCG 115 1.2770055E-4 24.79331 5 ATGGGAT 2020 0.0 24.701258 5 AGTACTT 6735 0.0 23.977571 12-13 ATGGGAG 1610 0.0 23.907837 5 CGCTGCG 90 1.9422805E-7 23.761248 94-95 TAGATAG 120 1.7044898E-4 23.760256 5 GATAGTC 100 0.0016517822 23.752333 7 ATGGGTA 225 2.7921487E-9 23.232252 5 CATGGGT 515 0.0 23.06821 4 GTATAGC 145 2.253192E-5 23.03313 1 GGAACGC 145 2.3301662E-5 22.933289 8 >>END_MODULE