##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938448_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600063 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.721840873374962 31.0 31.0 34.0 30.0 34.0 2 31.89727078656741 31.0 31.0 34.0 30.0 34.0 3 31.912205885048735 31.0 31.0 34.0 30.0 34.0 4 35.61175909862798 37.0 35.0 37.0 33.0 37.0 5 35.546980900338795 37.0 35.0 37.0 33.0 37.0 6 35.632801889134974 37.0 35.0 37.0 33.0 37.0 7 35.53621036457839 37.0 35.0 37.0 33.0 37.0 8 35.54876571293347 37.0 35.0 37.0 33.0 37.0 9 36.996655351188124 39.0 37.0 39.0 33.0 39.0 10-11 36.93770654081321 39.0 37.0 39.0 33.0 39.0 12-13 36.80895089348952 39.0 37.0 39.0 32.0 39.0 14-15 37.55436595824105 39.0 36.0 41.0 32.0 41.0 16-17 37.689700081491445 39.0 36.5 41.0 32.5 41.0 18-19 38.00631683673214 40.0 37.0 41.0 33.0 41.0 20-21 38.03456303754773 40.0 37.0 41.0 33.0 41.0 22-23 37.892825419997564 40.0 37.0 41.0 33.0 41.0 24-25 37.738714934931835 40.0 36.5 41.0 32.0 41.0 26-27 37.413702394581904 39.0 36.0 41.0 32.0 41.0 28-29 37.18550718841188 39.0 36.0 41.0 31.5 41.0 30-31 36.764286416592924 38.5 35.0 40.0 31.0 41.0 32-33 36.366584008679084 38.0 35.0 40.0 30.0 41.0 34-35 36.0980163749473 38.0 35.0 40.0 30.0 41.0 36-37 35.84448716218131 38.0 35.0 40.0 30.0 41.0 38-39 35.52216933888609 38.0 35.0 40.0 29.5 41.0 40-41 35.1691247419021 38.0 34.5 40.0 27.5 41.0 42-43 34.77166314203676 38.0 34.0 40.0 25.5 41.0 44-45 34.22832685901314 38.0 33.0 40.0 23.5 41.0 46-47 33.41582717148033 37.0 33.0 40.0 20.0 41.0 48-49 32.76333568308661 37.0 32.5 40.0 13.5 41.0 50-51 32.3145553050263 36.5 31.5 40.0 8.0 41.0 52-53 31.668511472962006 35.5 31.0 39.0 4.0 41.0 54-55 31.30451052639473 35.0 30.0 39.0 2.0 40.0 56-57 30.988538703436138 35.0 30.0 39.0 2.0 40.0 58-59 30.528484509126542 35.0 28.5 38.5 2.0 40.0 60-61 30.106863779303172 35.0 28.0 38.0 2.0 40.0 62-63 29.67496246227479 34.5 28.0 38.0 2.0 40.0 64-65 29.050723840663395 34.0 26.5 37.5 2.0 40.0 66-67 28.46826583208763 34.0 26.0 37.0 2.0 39.0 68-69 27.874032393265374 33.0 25.5 36.0 2.0 39.0 70-71 27.195521136947285 33.0 24.0 35.5 2.0 38.5 72-73 26.671554486778888 32.5 23.0 35.0 2.0 38.0 74-75 26.02747794814878 32.0 21.0 35.0 2.0 37.0 76-77 24.873354964395403 30.5 20.0 34.0 2.0 36.0 78-79 25.36685981305296 31.5 20.0 35.0 2.0 36.0 80-81 25.050198895782607 31.5 18.0 35.0 2.0 36.0 82-83 24.48230185830488 31.0 13.5 34.5 2.0 35.0 84-85 23.82031969976486 31.0 6.5 34.0 2.0 35.0 86-87 22.99804270551592 30.0 2.0 34.0 2.0 35.0 88-89 22.10230342480706 29.0 2.0 34.0 2.0 35.0 90-91 21.332185953808185 29.0 2.0 34.0 2.0 35.0 92-93 20.411800927569274 27.5 2.0 33.0 2.0 35.0 94-95 19.422810604886486 26.5 2.0 33.0 2.0 35.0 96-97 18.199590709642152 24.5 2.0 33.0 2.0 34.5 98-99 16.653538878417766 11.0 2.0 32.0 2.0 34.0 100-101 12.677683009950622 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25370857949133097 1101 2 0.3000823474142287 1101 3 0.3354715191163713 1101 4 0.10023962474537029 1101 5 0.08822879924977656 1101 6 0.18203609447042624 1101 7 0.2069707020392073 1101 8 0.25891158888315857 1101 9 0.14966911384394166 1101 10-11 0.23250466467949593 1101 12-13 0.30248509710219906 1101 14-15 0.37883546700594906 1101 16-17 0.24218221789180205 1101 18-19 0.21128298371306897 1101 20-21 0.3498816687361881 1101 22-23 0.3807698540953979 1101 24-25 0.4494081805931245 1101 26-27 0.39723011549956766 1101 28-29 0.45870458353684995 1101 30-31 0.37634097828394886 1101 32-33 0.39547475550926237 1101 34-35 0.3692661301948661 1101 36-37 0.4763860102530373 1101 38-39 0.6823061790810598 1101 40-41 0.909397865376306 1101 42-43 0.9633210428762737 1101 44-45 0.820836673270513 1101 46-47 1.0594669354096666 1101 48-49 0.9477115015815301 1101 50-51 0.8858673740674377 1101 52-53 1.090955094386306 1101 54-55 1.0626564562790044 1101 56-57 1.184638047022446 1101 58-59 1.316867629184948 1101 60-61 1.3113681461139777 1101 62-63 1.4322730062696003 1101 64-65 1.7464769574583094 1101 66-67 1.6432312903657547 1101 68-69 1.7158790367855872 1101 70-71 1.997083562742759 1101 72-73 1.8705926501964 1101 74-75 1.789043357507179 1101 76-77 1.6439390058154828 1101 78-79 1.4823725903544904 1101 80-81 2.036716768654834 1101 82-83 2.0567218166108283 1101 84-85 2.3045708329805983 1101 86-87 1.9583037089276267 1101 88-89 1.705805742108815 1101 90-91 1.8887198399148417 1101 92-93 2.1996998585477883 1101 94-95 1.9269049465606933 1101 96-97 2.6645520954829323 1101 98-99 2.4302224604420495 1101 100-101 2.060138029467984 1102 1 0.38109026147975555 1102 2 0.1648354968864787 1102 3 0.2547360747854235 1102 4 0.0956294783374716 1102 5 0.1781981557359913 1102 6 0.16528430693212215 1102 7 -0.015256059962837298 1102 8 0.10986834748037211 1102 9 0.44338378868221184 1102 10-11 0.5436384746897076 1102 12-13 0.4145177835169065 1102 14-15 0.6800612075575359 1102 16-17 0.6449980163255802 1102 18-19 0.5648818941659073 1102 20-21 0.3105864695533498 1102 22-23 0.4921896702343176 1102 24-25 0.328424183316983 1102 26-27 0.35386954348731336 1102 28-29 0.403137678531742 1102 30-31 0.2530655271433204 1102 32-33 0.13397662816844758 1102 34-35 0.21934784623163495 1102 36-37 0.15509898267387 1102 38-39 0.25153328156658716 1102 40-41 0.4021387574430477 1102 42-43 0.4563309168973859 1102 44-45 0.6329578853917255 1102 46-47 0.6141315591754122 1102 48-49 0.30902917267447805 1102 50-51 0.48842100021656876 1102 52-53 0.46625642227190056 1102 54-55 0.4828981995544588 1102 56-57 0.5259727422897242 1102 58-59 0.3251992601008489 1102 60-61 0.5013579316093804 1102 62-63 0.19314123916030468 1102 64-65 0.6830346694092775 1102 66-67 0.5824447735118206 1102 68-69 0.6659198948039702 1102 70-71 0.9602092011492971 1102 72-73 1.1347942164204383 1102 74-75 0.7621553765742526 1102 76-77 0.9101766632890964 1102 78-79 1.1672210752029706 1102 80-81 1.5216027066868385 1102 82-83 1.6955880066006124 1102 84-85 1.940502055316312 1102 86-87 1.8092298240110445 1102 88-89 1.6003988643423845 1102 90-91 2.1559993768572987 1102 92-93 1.8925394908256195 1102 94-95 1.8658358284129228 1102 96-97 2.1231833518669987 1102 98-99 1.7075530699074868 1102 100-101 1.712974256910952 1103 1 0.3242377291263452 1103 2 0.19040507669155815 1103 3 0.2974489682188768 1103 4 0.21582120225502877 1103 5 0.095248802804214 1103 6 0.25601765091678175 1103 7 0.35329482676028334 1103 8 0.3461169494934708 1103 9 0.45656800341300396 1103 10-11 0.4982478372076855 1103 12-13 0.32144700543734217 1103 14-15 0.592323549784723 1103 16-17 0.2488500693691904 1103 18-19 0.18896082051197283 1103 20-21 0.36944091914593713 1103 22-23 0.3933831516211157 1103 24-25 0.5124088454035842 1103 26-27 0.544507025513191 1103 28-29 0.4198576018421747 1103 30-31 0.4200522662390256 1103 32-33 0.43580439618249756 1103 34-35 0.46873592739476777 1103 36-37 0.6020697447764505 1103 38-39 0.6118560168278151 1103 40-41 0.4641278930877135 1103 42-43 0.6311527734239917 1103 44-45 0.6654471009213694 1103 46-47 1.1535787552131254 1103 48-49 0.8497177810783754 1103 50-51 0.8588635629262669 1103 52-53 0.673506781754245 1103 54-55 0.9907269261881062 1103 56-57 1.0982686362024907 1103 58-59 1.2308487248715778 1103 60-61 0.990773173660827 1103 62-63 1.3111409196204455 1103 64-65 1.6009624534003315 1103 66-67 1.3510915221937196 1103 68-69 1.3084522482150618 1103 70-71 1.1083718469393595 1103 72-73 1.3178554083041263 1103 74-75 1.2247756034475543 1103 76-77 1.572330361963008 1103 78-79 1.5339250215202753 1103 80-81 1.9726300273450903 1103 82-83 2.101611333010581 1103 84-85 1.9815453529676965 1103 86-87 1.9989262611415732 1103 88-89 1.5267545122094575 1103 90-91 1.7893273845262563 1103 92-93 1.6054185449876286 1103 94-95 1.7999738696335648 1103 96-97 1.6898872981842992 1103 98-99 2.011572881378971 1103 100-101 2.294551186132873 1104 1 0.07364276799516034 1104 2 0.2655376278078343 1104 3 0.25243345329224454 1104 4 0.07235184534651751 1104 5 0.12738411311756437 1104 6 -0.029978608904151827 1104 7 0.0365176637839042 1104 8 0.09223229045139902 1104 9 0.12258660947019706 1104 10-11 0.2593061122797735 1104 12-13 0.13590211587931122 1104 14-15 0.00950318210911405 1104 16-17 0.1922109551573996 1104 18-19 0.2391376047285334 1104 20-21 0.2545371057636814 1104 22-23 0.33540444871048436 1104 24-25 0.0527565457269219 1104 26-27 0.20039721480419104 1104 28-29 0.125742308303316 1104 30-31 0.26448511935549845 1104 32-33 0.4767759536321847 1104 34-35 0.16296887949854266 1104 36-37 0.30008288578594033 1104 38-39 0.4538512334292264 1104 40-41 0.6457429273035373 1104 42-43 0.4497955803957723 1104 44-45 0.4390210465139077 1104 46-47 0.6216894313201351 1104 48-49 0.6561072042550435 1104 50-51 0.44782618188144596 1104 52-53 0.7774567902007767 1104 54-55 0.9429625871061909 1104 56-57 0.7486463587238816 1104 58-59 0.7412838837402802 1104 60-61 0.6087088437663617 1104 62-63 0.3083980228487917 1104 64-65 0.5822482214558811 1104 66-67 0.6548225138153612 1104 68-69 0.7609175952485536 1104 70-71 0.6090594234396569 1104 72-73 1.0459026021325357 1104 74-75 0.6929203620104012 1104 76-77 0.9161156484186392 1104 78-79 0.7409448219452663 1104 80-81 1.0473669256045142 1104 82-83 1.1863284634456193 1104 84-85 1.5256361960491027 1104 86-87 1.4741186788324434 1104 88-89 2.0787554487360715 1104 90-91 2.15592272500999 1104 92-93 1.9622574120275225 1104 94-95 1.980689423384561 1104 96-97 1.6068565083900666 1104 98-99 1.8578550781858887 1104 100-101 1.3299035839077327 1105 1 0.22760315157473343 1105 2 0.3057189583669562 1105 3 0.3454310845849129 1105 4 0.2814217292043466 1105 5 0.21036158103374447 1105 6 0.02451843930057862 1105 7 0.19914466369161943 1105 8 0.3236720562094533 1105 9 0.5002569983912437 1105 10-11 0.4374723356877439 1105 12-13 0.4761625897885793 1105 14-15 0.5470067827120602 1105 16-17 0.4661000817559966 1105 18-19 0.48046609437697896 1105 20-21 0.5723368087663729 1105 22-23 0.5261245413333668 1105 24-25 0.48818899561911167 1105 26-27 0.5803733879016022 1105 28-29 0.5390565689282383 1105 30-31 0.48151268529197466 1105 32-33 0.4762320178865167 1105 34-35 0.33227412393403455 1105 36-37 0.323624536721816 1105 38-39 0.4237208286795138 1105 40-41 0.4175360135865631 1105 42-43 0.4944388620202531 1105 44-45 0.7557454626569182 1105 46-47 0.8971558317519239 1105 48-49 0.9928509727649342 1105 50-51 0.8642991850499797 1105 52-53 0.7198611260258652 1105 54-55 0.9582487196810909 1105 56-57 0.9655928237008489 1105 58-59 0.8748781384364435 1105 60-61 0.6523831087539591 1105 62-63 0.6980741503618724 1105 64-65 0.8347714401012745 1105 66-67 0.9279174103277441 1105 68-69 0.8567943106037603 1105 70-71 1.0051285438298372 1105 72-73 1.4701417213201857 1105 74-75 1.291023158874868 1105 76-77 1.1539637882702536 1105 78-79 1.2419082454125316 1105 80-81 1.2169532268134802 1105 82-83 1.3571438636788535 1105 84-85 1.8941323884988854 1105 86-87 1.7281156976730365 1105 88-89 1.6254584150735774 1105 90-91 1.5961471229751965 1105 92-93 1.724240078794864 1105 94-95 1.678814872585999 1105 96-97 1.8456317161064035 1105 98-99 1.683404117698835 1105 100-101 1.5037672615845548 1106 1 -0.03421276882327717 1106 2 0.008471860522842434 1106 3 0.028523953573301952 1106 4 0.08093250864050106 1106 5 0.10092542846326324 1106 6 -0.012139935492122333 1106 7 0.05292575821898282 1106 8 -0.0919498343378109 1106 9 -0.24388893859051564 1106 10-11 0.09522938378061951 1106 12-13 0.05360869260780987 1106 14-15 -0.18486303486671574 1106 16-17 -0.11560804428048499 1106 18-19 0.17374553052955122 1106 20-21 -0.1663074698405893 1106 22-23 0.12749270053735273 1106 24-25 0.03349994089274588 1106 26-27 -0.4195395474217847 1106 28-29 -0.08224110934705209 1106 30-31 0.16541401199181394 1106 32-33 -0.061023371831559814 1106 34-35 -0.184591547707754 1106 36-37 -0.23285556278067077 1106 38-39 -0.1371788396455358 1106 40-41 0.20819936350364543 1106 42-43 0.018452129018598384 1106 44-45 -0.06582999339614304 1106 46-47 -0.6210199559761023 1106 48-49 -0.31824355459824716 1106 50-51 0.19031493961050927 1106 52-53 -0.21639509287960834 1106 54-55 0.12804971470596982 1106 56-57 0.011351530168511204 1106 58-59 0.30451744191903174 1106 60-61 0.2772670225184726 1106 62-63 0.0700351785542459 1106 64-65 0.04106497243958174 1106 66-67 -0.2650552264881796 1106 68-69 -0.11188313549905615 1106 70-71 -0.5721392836991868 1106 72-73 -0.36414517751895303 1106 74-75 -0.5773143203954447 1106 76-77 -0.2783081851957512 1106 78-79 -0.6342940763121767 1106 80-81 -0.40491244482831235 1106 82-83 -0.22683623582362955 1106 84-85 -0.25237673256247106 1106 86-87 -0.23819441841319744 1106 88-89 0.11327765222117492 1106 90-91 -0.05771274435481999 1106 92-93 -0.42018778190165307 1106 94-95 -0.5948459897688956 1106 96-97 -0.6733317996481532 1106 98-99 -0.05820450585008885 1106 100-101 0.351383347820045 1107 1 -0.034077046782016396 1107 2 0.033097267688965815 1107 3 0.13673785151032902 1107 4 0.11379352927024655 1107 5 0.2012837346521863 1107 6 -0.02002810053012638 1107 7 0.1204501881864104 1107 8 -0.0275198669110992 1107 9 -0.22701597442113552 1107 10-11 -0.05476247289691116 1107 12-13 -0.03857371781564467 1107 14-15 -0.0032968025105830634 1107 16-17 0.012937015437209709 1107 18-19 -0.08693036523592212 1107 20-21 0.34489502744497 1107 22-23 0.22833417719315463 1107 24-25 0.04190656412836091 1107 26-27 -0.029873423643280717 1107 28-29 -0.0931015870886327 1107 30-31 0.14913008148149487 1107 32-33 0.09560529266355644 1107 34-35 0.28249857172763626 1107 36-37 0.1953773362421245 1107 38-39 0.43535916252601936 1107 40-41 0.19040348945370056 1107 42-43 0.07207862196105452 1107 44-45 0.22773135296650082 1107 46-47 0.10397461514224915 1107 48-49 0.0317890186916614 1107 50-51 0.06342840323700472 1107 52-53 0.3321255368706666 1107 54-55 0.11667077876677467 1107 56-57 0.06846607957133344 1107 58-59 0.32405327253792393 1107 60-61 0.3202800518344304 1107 62-63 0.4956079255683612 1107 64-65 0.41917029274359763 1107 66-67 0.060718389146998675 1107 68-69 0.33251154419692597 1107 70-71 0.48258112889581994 1107 72-73 0.7127006422421758 1107 74-75 0.4813203158694854 1107 76-77 0.49679496355560815 1107 78-79 0.6441532635358129 1107 80-81 0.9445935269415031 1107 82-83 1.1518065437637723 1107 84-85 1.1812986203148341 1107 86-87 1.5715721396758333 1107 88-89 1.188918260255921 1107 90-91 1.171323629152237 1107 92-93 1.2905939770560018 1107 94-95 1.1941035216317566 1107 96-97 1.0598875271705204 1107 98-99 1.5233155810120174 1107 100-101 1.5264240644324207 1108 1 -0.07429965665897953 1108 2 -0.05054867660196294 1108 3 -0.26682359776950904 1108 4 0.009597595528333613 1108 5 -0.01931153836138577 1108 6 -0.008735512111400112 1108 7 -0.0847203713228808 1108 8 -0.06221839673907681 1108 9 -0.1555477063630235 1108 10-11 -0.01994044244371196 1108 12-13 0.06751074632028775 1108 14-15 0.05920094617594174 1108 16-17 -0.2594153112631048 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0.09660458712029296 2316 98-99 -0.46611799185517455 2316 100-101 -1.0990937514165982 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 22.0 5 75.0 6 169.0 7 366.0 8 606.0 9 1040.0 10 1767.0 11 2652.0 12 3789.0 13 5028.0 14 6377.0 15 8422.0 16 11087.0 17 14216.0 18 12409.0 19 8318.0 20 7554.0 21 8160.0 22 9092.0 23 9972.0 24 11090.0 25 12708.0 26 14407.0 27 16658.0 28 19667.0 29 22183.0 30 26142.0 31 30141.0 32 35814.0 33 42317.0 34 53450.0 35 65254.0 36 64303.0 37 56028.0 38 18398.0 39 369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.92637635935698 18.9136479107075 14.930256902633035 18.229718827302488 2 15.627573011010403 23.4678604882179 39.01959738523746 21.884969115534236 3 20.386430599810684 30.3103377018145 30.276174223739115 19.0270574746357 4 12.056234095419981 17.86212447692992 36.018718034606366 34.06292339304373 5 11.777488917774889 41.42752391427524 30.989234409892347 15.805752758057528 6 27.50177897987378 36.66731659842383 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19.162269898777126 26.441184410944203 35.7968843219534 18.599661368325275 30-31 18.869518700536446 27.99764358075735 34.81884402137776 18.31399369732845 32-33 18.498119357865285 27.976155757497988 34.285847605493444 19.239877279143286 34-35 19.03247231057819 27.195424693355957 34.32888308151536 19.4432199145505 36-37 19.902959138014502 27.003207197988853 34.25885729093844 18.834976373058204 38-39 20.31013669314751 26.47187011192621 34.599708163505106 18.618285031421173 40-41 19.043726161279544 27.39445545841594 34.850428044877 18.711390335427517 42-43 18.73246971416572 28.14411874013596 33.89446913753077 19.22894240816755 44-45 18.859557391678948 28.55956064137294 32.74866616726925 19.832215799678863 46-47 19.0253673787042 28.452143278527885 31.631648063033484 20.890841279734428 48-49 19.691849022519303 28.224786397428268 31.418701036391177 20.66466354366125 50-51 20.185880482549333 28.983623386211114 30.894506076861926 19.935990054377623 52-53 19.40604569853499 29.299840183447408 30.993329033784782 20.30078508423282 54-55 18.441396986649735 29.109943455937128 31.14281333793285 21.305846219480287 56-57 19.53269906659801 29.721295930593953 29.67755052386166 21.06845447894638 58-59 19.177319714763282 28.762146641269332 30.371810959849217 21.68872268411817 60-61 19.38004842824837 28.461094918366904 30.8008492441627 21.358007409222033 62-63 20.67766217880456 28.3016116641086 30.36789470438937 20.652831452697466 64-65 19.901026893887632 28.54796671010662 30.991880838979974 20.559125557025773 66-67 19.21282871712829 28.77837216278372 30.865413458654135 21.143385661433854 68-69 19.275319448552846 29.31552392895681 30.414585268911733 20.994571353578614 70-71 19.084746101659324 29.41249502135609 29.53406558977974 21.968693287204843 72-73 19.319586976012477 28.841936333245567 29.129573277428 22.70890341331396 74-75 19.20873308302628 29.05061635194971 29.376498800959233 22.364151764064776 76-77 19.20031730001683 28.18195756112275 30.72894012795323 21.88878501090719 78-79 18.795609794304934 28.183374079055035 30.899672201085554 22.121343925554484 80-81 18.2968440787418 29.440099989584418 30.290594729715654 21.97246120195813 82-83 18.22063274094418 28.97912861466263 29.461000306638045 23.33923833775514 84-85 18.4096122901853 27.92315634304323 29.615423395916995 24.051807970854476 86-87 18.844275122117065 27.779277680284114 29.767231796599887 23.609215400998934 88-89 19.318721534238907 27.184895585963474 29.835450611019176 23.660932268778446 90-91 18.46477662084872 27.445188449078085 30.406828695988374 23.68320623408482 92-93 17.90108797725555 28.081512052574876 29.996825309532323 24.020574660637255 94-95 17.800743923127943 27.95669447490426 29.553779442790912 24.688782159176885 96-97 18.058520522011857 27.35704417702808 29.075863700978065 25.508571599981998 98-99 18.521555236700145 27.03449471138864 29.019036334518212 25.424913717393004 100-101 18.861951880012498 26.83620456202479 29.369524007915842 24.93231955004687 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3233.0 1 2213.0 2 1628.5 3 2067.5 4 1710.0 5 1642.5 6 3070.5 7 6348.5 8 8990.5 9 8776.5 10 6333.5 11 4188.0 12 3664.5 13 3499.5 14 3304.5 15 3040.0 16 2852.0 17 2735.0 18 2644.0 19 2571.5 20 2623.0 21 2725.5 22 2763.0 23 3038.5 24 3359.5 25 3677.0 26 4093.5 27 4490.5 28 4917.5 29 5582.0 30 6154.5 31 6498.5 32 7001.0 33 7623.0 34 8604.5 35 9444.0 36 10241.0 37 11068.0 38 11842.0 39 12394.5 40 12772.0 41 13739.0 42 14736.0 43 15742.5 44 16602.0 45 16987.0 46 18208.0 47 21463.0 48 31145.5 49 32470.0 50 25880.0 51 23200.5 52 19265.5 53 16751.5 54 15458.5 55 14336.0 56 13055.5 57 12100.0 58 11281.5 59 10362.0 60 9286.0 61 8173.0 62 6923.5 63 4987.0 64 3364.5 65 2603.5 66 1968.0 67 1471.0 68 1110.0 69 810.5 70 591.0 71 436.5 72 407.5 73 405.0 74 322.0 75 216.0 76 138.0 77 98.0 78 72.0 79 40.5 80 21.5 81 10.0 82 5.0 83 3.5 84 3.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02449742777008414 2 0.014165179322837769 3 0.0011665441795278163 4 0.0 5 4.999475055119212E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002249763774803646 26-27 0.009249028851970544 28-29 1.6664916850397376E-4 30-31 0.0 32-33 3.3329833700794753E-4 34-35 0.009915625525986438 36-37 0.0010832195952758293 38-39 0.011582117211026175 40-41 0.011248818874018228 42-43 0.004999475055119212 44-45 0.0010832195952758293 46-47 1.6664916850397376E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.6664916850397376E-4 66-67 4.999475055119212E-4 68-69 9.165704267718556E-4 70-71 0.0 72-73 1.6664916850397376E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.332458425198688E-5 82-83 0.0011665441795278163 84-85 0.006582642155906964 86-87 0.00399958004409537 88-89 0.0 90-91 0.0011665441795278163 92-93 7.499212582678819E-4 94-95 1.6664916850397376E-4 96-97 0.0 98-99 0.0 100-101 8.332458425198688E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 600063.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.56098947292277 #Duplication Level Percentage of deduplicated Percentage of total 1 72.55834205998552 31.60713174647759 2 13.48359680867666 11.747176372797979 3 5.438802390214329 7.107588409963007 4 2.702278891289206 4.70855769345402 5 1.6029979562546037 3.4914088548761755 6 0.9708834688977362 2.5375586740853415 7 0.6859090024678222 2.091521238511863 8 0.46291536483764345 1.613204106763743 9 0.3475892414944056 1.3627198160675111 >10 1.5735973432494783 11.932590898600267 >50 0.09535128490426747 2.904181197256118 >100 0.06356752296996604 5.419025275056347 >500 0.006126990165779859 1.8305849710173108 >1k 0.006892863936502341 6.626904423510544 >5k 7.658737707224824E-4 2.9553921935040726 >10k+ 3.829368853612412E-4 2.0644541280581588 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12376 2.0624501094051793 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9568 1.5944992442460209 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8149 1.358024074138882 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4859 0.8097483097608084 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4789 0.7980828679655303 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4248 0.7079256678048804 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2896 0.482615991987508 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2634 0.43895390983946686 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2101 0.3501299030268489 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1845 0.30746771588983157 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1844 0.3073010667213276 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1843 0.3071344175528236 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1826 0.30430138168825605 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1631 0.2718047938299812 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1545 0.25747296533863945 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1503 0.2504737002614726 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1412 0.23530862592761093 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1402 0.2336421342425712 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1195 0.19914575636224863 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1134 0.18898015708350624 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1020 0.16998215187405324 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 983 0.1638161326394062 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 887 0.14781781246302472 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 859 0.14315163574491346 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 845 0.1408185473858578 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 790 0.13165284311813927 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 715 0.11915415548034124 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 703 0.11715436545829355 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 675 0.1124881887401823 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 643 0.10715541534805512 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 613 0.10215594029293591 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.332458425198687E-5 0.0 12-13 0.0 0.0 0.0 1.6664916850397374E-4 0.0 14-15 0.0 0.0 0.0 1.6664916850397374E-4 0.0 16-17 0.0 0.0 0.0 4.999475055119212E-4 0.0 18-19 0.0 0.0 0.0 7.499212582678819E-4 0.0 20-21 0.0 0.0 0.0 0.0011665441795278163 0.0 22-23 0.0 0.0 0.0 0.003083009617323514 0.0 24-25 0.0 0.0 0.0 0.008582432177954648 0.0 26-27 0.0 0.0 0.0 0.019581277299216916 0.0 28-29 0.0 0.0 0.0 0.03674614165512621 0.0 30-31 0.0 0.0 0.0 0.08290796133072695 0.0 32-33 0.0 0.0 0.0 0.1567335429779873 0.0 34-35 0.0 0.0 0.0 0.2756377247055726 0.0 36-37 0.0 0.0 0.0 0.4385372869182069 0.0 38-39 0.0 0.0 0.0 0.6685964640379427 0.0 40-41 0.0 0.0 0.0 0.9248195606128022 0.0 42-43 0.0 0.0 0.0 1.1577117735971056 0.0 44-45 0.0 0.0 0.0 1.4074355526003104 0.0 46-47 0.0 0.0 0.0 1.7023212562680918 0.0 48-49 0.0 0.0 0.0 2.025703967750053 0.0 50-51 0.0 0.0 0.0 2.3768337657879255 0.0 52-53 0.0 0.0 0.0 2.6980500380793346 0.0 54-55 0.0 0.0 0.0 3.027182145874683 0.0 56-57 0.0 0.0 0.0 3.386144454832243 0.0 58-59 0.0 0.0 0.0 3.8117664311913915 0.0 60-61 0.0 0.0 0.0 4.332545082766309 0.0 62-63 0.0 0.0 0.0 4.793163384511293 0.0 64-65 0.0 0.0 0.0 5.204870155300361 0.0 66-67 0.0 0.0 0.0 5.652073198980774 0.0 68-69 0.0 0.0 0.0 6.172268578465928 0.0 70-71 0.0 0.0 0.0 6.759040300768419 0.0 72-73 0.0 0.0 0.0 7.340979197184296 0.0 74-75 0.0 0.0 0.0 7.908586265108831 0.0 76-77 0.0 0.0 0.0 8.445446561444381 0.0 78-79 0.0 0.0 0.0 9.055382518168926 0.0 80-81 0.0 0.0 0.0 9.72281243802734 0.0 82-83 0.0 0.0 0.0 10.380660030696777 0.0 84-85 0.0 0.0 0.0 11.087252505153625 0.0 86-87 0.0 0.0 0.0 11.820425521986857 0.0 88-89 0.0 0.0 0.0 12.50277054242638 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 60 0.0039464426 31.67161 2 TAGATAG 90 2.4112289E-5 31.666332 5 GTAAGGT 105 1.8998271E-6 31.666332 3 TCGTATC 60 5.382377E-6 27.708042 94-95 GTATAGA 140 5.7862053E-7 27.149359 1 GTATTAG 105 6.8891866E-5 27.149357 1 AGACCTA 70 0.00838886 27.142572 5 CGATGTA 35 0.008392145 27.142572 60-61 ATGGGCG 125 7.3838055E-6 26.599718 5 GGACCGC 90 8.9430937E-4 26.38861 6 TCTAGAC 95 0.0012259468 24.999737 3 GAGTACT 7445 0.0 24.786509 12-13 CATGGGT 540 0.0 24.629368 4 AGTACTT 7475 0.0 24.019815 12-13 GTATGGA 100 0.0016504256 23.755688 1 GTATCTA 100 0.0016504256 23.755688 1 GTATAGG 100 0.0016504256 23.755688 1 CTAGACT 120 1.709611E-4 23.74975 4 CGGGAGT 380 0.0 23.74975 4 GTACATG 9255 0.0 23.357834 1 >>END_MODULE