FastQCFastQC Report
Thu 26 May 2016
SRR938447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1852489
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56190.3033216391568317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43490.2347652266761098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28600.15438688164949968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33700.047.5581171
GTATCAA63100.044.8448141
ATCAACG80400.034.8385733
TCAACGC82000.034.0428124
CAACGCA83700.033.2377435
TATCAAC85350.033.040932
AACGCAG86100.032.5102126
ACGCAGA98300.028.2321197
CGCAGAG99600.027.6727818
GTACATG73800.026.292291
TACATGG74200.025.7005022
GCAGAGT105750.024.9393449
ACATGGG75900.023.9970653
GAGTACT59950.021.67672512-13
AGTACTT62850.020.97892412-13
AGAGTAC99000.020.7355310-11
ATAACGC1150.003698993520.6763233
GTACTTT64250.020.0729314-15
GGACCGA6900.019.973616
CAGAGTA105550.019.60633910-11
Produced by FastQC (version 0.11.5)