##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938447_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1852489 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.789220880663798 31.0 31.0 34.0 27.0 34.0 2 30.824605166346466 31.0 31.0 34.0 27.0 34.0 3 30.856025595833497 31.0 31.0 34.0 27.0 34.0 4 34.5078486296005 37.0 35.0 37.0 30.0 37.0 5 34.47989272810797 37.0 35.0 37.0 30.0 37.0 6 34.48497777854551 37.0 35.0 37.0 30.0 37.0 7 34.37665864682597 37.0 35.0 37.0 30.0 37.0 8 34.33563006312048 37.0 35.0 37.0 30.0 37.0 9 35.77940003962237 38.0 35.0 39.0 30.0 39.0 10-11 35.59115060872156 38.0 35.0 39.0 30.0 39.0 12-13 35.42836178784327 38.0 35.0 39.0 29.5 39.0 14-15 36.31314436954821 39.0 35.5 40.5 28.5 41.0 16-17 36.123560517768254 38.0 35.0 40.0 27.0 41.0 18-19 36.27892500306345 39.0 36.0 40.5 29.0 41.0 20-21 36.25158637919037 39.0 35.0 41.0 27.0 41.0 22-23 36.148886174222895 39.0 35.0 41.0 27.0 41.0 24-25 35.99018914552259 39.0 35.0 41.0 27.0 41.0 26-27 35.76105715067674 39.0 34.5 40.0 27.0 41.0 28-29 35.53066711867115 38.0 34.0 40.0 27.0 41.0 30-31 35.26126848796403 38.0 34.0 40.0 25.5 41.0 32-33 34.96250153172299 38.0 34.0 40.0 25.0 41.0 34-35 34.67971334782554 38.0 33.5 40.0 24.5 41.0 36-37 34.38124436906238 38.0 33.0 40.0 24.0 41.0 38-39 34.20532834472971 38.0 33.0 40.0 23.5 41.0 40-41 34.08211249837381 38.0 33.0 40.0 23.0 41.0 42-43 33.76460912858322 38.0 33.0 40.0 21.5 41.0 44-45 33.386259783458904 38.0 32.0 40.0 18.5 41.0 46-47 32.9578359169744 37.0 31.0 40.0 16.0 41.0 48-49 32.56530942963764 37.0 31.0 40.0 14.0 41.0 50-51 31.874824088024276 36.5 30.5 39.5 9.5 40.5 52-53 31.907840478405 36.5 30.5 39.0 9.0 40.0 54-55 31.994833707514594 37.0 31.0 39.0 8.0 41.0 56-57 31.65106972295112 36.0 30.0 39.0 7.0 41.0 58-59 31.26007333916693 36.0 29.5 39.0 2.0 40.5 60-61 30.757781557677266 35.0 28.5 39.0 2.0 40.0 62-63 30.159656278660766 35.0 28.0 38.5 2.0 40.0 64-65 29.613655735607608 34.5 27.5 38.0 2.0 40.0 66-67 29.04819623760249 34.0 26.0 38.0 2.0 40.0 68-69 28.419220303062527 34.0 26.0 37.0 2.0 39.5 70-71 27.76178293096477 33.5 25.0 36.5 2.0 39.0 72-73 27.09952258825828 33.0 23.5 36.0 2.0 39.0 74-75 26.392205837659496 32.0 21.5 35.5 2.0 38.0 76-77 25.613628205079763 31.5 19.0 35.0 2.0 37.5 78-79 24.801028238224355 31.0 15.0 35.0 2.0 37.0 80-81 24.07652704010658 30.5 7.5 34.0 2.0 36.0 82-83 23.312004011899667 30.0 4.5 34.0 2.0 36.0 84-85 22.579567543990812 29.5 2.0 34.0 2.0 35.0 86-87 21.778361436963998 29.0 2.0 34.0 2.0 35.0 88-89 20.919213555384133 28.0 2.0 34.0 2.0 35.0 90-91 19.98277452659638 26.5 2.0 33.5 2.0 35.0 92-93 19.01227402699827 25.5 2.0 33.0 2.0 35.0 94-95 17.988785898323822 24.0 2.0 32.0 2.0 34.0 96-97 16.741336655710235 17.5 2.0 32.0 2.0 34.0 98-99 15.255180732517172 2.0 2.0 31.5 2.0 34.0 100-101 12.018972582293337 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6016078035096974 1101 2 0.5780433043292987 1101 3 0.5888476279508303 1101 4 0.40629488964794547 1101 5 0.4404376515371453 1101 6 0.6201792491226428 1101 7 0.5613386158052265 1101 8 0.5402096505953295 1101 9 0.7752062498806325 1101 10-11 0.7622542457833745 1101 12-13 0.7835298066342276 1101 14-15 0.9223634017134614 1101 16-17 0.7030991803035533 1101 18-19 0.9259106233232544 1101 20-21 0.8696441509056214 1101 22-23 0.8894649253569824 1101 24-25 0.9703948104689246 1101 26-27 0.8553949855036862 1101 28-29 1.1567536797423514 1101 30-31 1.0146612352945041 1101 32-33 1.0320445153396705 1101 34-35 0.9562329540128189 1101 36-37 1.1127597949765615 1101 38-39 1.1528680073930033 1101 40-41 1.1522202137541555 1101 42-43 1.3664203348059232 1101 44-45 1.4483288590367707 1101 46-47 1.4168498933198137 1101 48-49 1.3767774533354853 1101 50-51 1.3971298702557569 1101 52-53 1.3606857249791346 1101 54-55 1.4021447369155382 1101 56-57 1.325113991093616 1101 58-59 1.3502551742378763 1101 60-61 1.5717901963186556 1101 62-63 1.3089688810129516 1101 64-65 1.4113174166079396 1101 66-67 1.4142260964159306 1101 68-69 1.3106486473245944 1101 70-71 1.3548136075295751 1101 72-73 1.4709262199136717 1101 74-75 1.358964915930283 1101 76-77 1.5106230259762867 1101 78-79 1.7539537401589875 1101 80-81 1.7022748764398443 1101 82-83 1.7399675721411256 1101 84-85 1.498158174557613 1101 86-87 1.5449834687443982 1101 88-89 1.6888970846571887 1101 90-91 1.5740454867866838 1101 92-93 1.4982511324317684 1101 94-95 1.1849017388958067 1101 96-97 1.4544625768792798 1101 98-99 1.7465165523344677 1101 100-101 1.4054427705283263 1102 1 0.6577099177181864 1102 2 0.5554543342519551 1102 3 0.6685847453279585 1102 4 0.5762867199004447 1102 5 0.5530140997585065 1102 6 0.5492971454508364 1102 7 0.602685555204026 1102 8 0.7026330114920185 1102 9 0.8827625027915857 1102 10-11 0.9110534074298471 1102 12-13 0.9584988837581747 1102 14-15 0.972063335286542 1102 16-17 1.14018926131385 1102 18-19 1.1024544765426683 1102 20-21 0.9945286673252909 1102 22-23 1.1085792712808953 1102 24-25 1.0919646618100671 1102 26-27 0.9585628623619229 1102 28-29 0.7216535430708717 1102 30-31 1.0336950290351012 1102 32-33 1.022943263917604 1102 34-35 0.9656997443709869 1102 36-37 1.220534607721369 1102 38-39 0.8994731718950746 1102 40-41 1.0559279047697245 1102 42-43 0.9774305966196053 1102 44-45 1.100072374776893 1102 46-47 0.959263862824514 1102 48-49 1.2169214546793015 1102 50-51 1.2328377750591066 1102 52-53 0.8114983094444028 1102 54-55 0.6286023114931183 1102 56-57 0.860647620675735 1102 58-59 0.9865067108375847 1102 60-61 1.1223080590516226 1102 62-63 1.1140726826178877 1102 64-65 1.3186356397260361 1102 66-67 1.1579899830778615 1102 68-69 1.361660894310564 1102 70-71 1.295674561481217 1102 72-73 1.4074331565637692 1102 74-75 1.4287513572035415 1102 76-77 1.4291746519087454 1102 78-79 1.4857197417929378 1102 80-81 1.5965358197020905 1102 82-83 1.562291788952482 1102 84-85 1.6694260506523229 1102 86-87 1.7998375967574702 1102 88-89 1.832486123452039 1102 90-91 1.8823370169727554 1102 92-93 1.7834488097801895 1102 94-95 1.9052911762521703 1102 96-97 1.9675465802082606 1102 98-99 2.196525523786166 1102 100-101 1.8267879538131773 1103 1 0.6273115929522213 1103 2 0.5989335746728806 1103 3 0.5913703516625723 1103 4 0.6170313441358033 1103 5 0.585795090553006 1103 6 0.5745389132879524 1103 7 0.5869662311918873 1103 8 0.6414548796531889 1103 9 0.8519896981872463 1103 10-11 0.9177646645982449 1103 12-13 0.8907559376796144 1103 14-15 0.9614981325620349 1103 16-17 0.9797522037632973 1103 18-19 1.0009273599400004 1103 20-21 0.8696021932447735 1103 22-23 0.9485075599141268 1103 24-25 0.9897013726470831 1103 26-27 1.13839569212643 1103 28-29 1.1515774049261722 1103 30-31 1.2631018347038179 1103 32-33 1.3555507761658347 1103 34-35 1.4464108359157848 1103 36-37 1.3474977345488597 1103 38-39 1.341508411970132 1103 40-41 1.3924491880116605 1103 42-43 1.5289756151852814 1103 44-45 1.4028030939365053 1103 46-47 1.0332272843645924 1103 48-49 0.9870663387975824 1103 50-51 1.2147385251148144 1103 52-53 1.3907651875247211 1103 54-55 1.3560566462646975 1103 56-57 1.363812888675426 1103 58-59 1.2556861676652353 1103 60-61 1.33710954211357 1103 62-63 1.4078079698226098 1103 64-65 1.4004212752883483 1103 66-67 1.3983966012022009 1103 68-69 1.469868061428432 1103 70-71 1.733873514399054 1103 72-73 1.6913123170956652 1103 74-75 1.771684465970754 1103 76-77 1.741774437085187 1103 78-79 1.7826129900736767 1103 80-81 1.6157959571453233 1103 82-83 1.648921306001359 1103 84-85 1.6705575582289924 1103 86-87 1.8213245191908172 1103 88-89 2.0770796088424532 1103 90-91 2.132470368329834 1103 92-93 2.1230151012363265 1103 94-95 2.0173268669208646 1103 96-97 2.3146901515159186 1103 98-99 2.429689610499862 1103 100-101 2.3891635077693287 1104 1 0.618380498765088 1104 2 0.5306888197470805 1104 3 0.5818762327681029 1104 4 0.33131540831529094 1104 5 0.4916334287149411 1104 6 0.6441368822525817 1104 7 0.6539078253011326 1104 8 0.7519078287357388 1104 9 0.7640500369024394 1104 10-11 0.8309744503882257 1104 12-13 0.9792602526227796 1104 14-15 1.0138410676237513 1104 16-17 1.177461987480072 1104 18-19 0.9553417579715102 1104 20-21 1.089058028828525 1104 22-23 1.0518174106844427 1104 24-25 0.8898607432914929 1104 26-27 1.0704318572084688 1104 28-29 1.140853724183991 1104 30-31 1.0726516933404824 1104 32-33 0.9984601300764737 1104 34-35 1.0069970035745257 1104 36-37 1.0785942022129973 1104 38-39 1.3267781040628535 1104 40-41 1.303223988198603 1104 42-43 1.309817285292489 1104 44-45 1.3270084472026653 1104 46-47 1.1784910648459643 1104 48-49 1.0697262736513906 1104 50-51 1.1045048920742424 1104 52-53 1.1775923641142754 1104 54-55 1.2388783507635068 1104 56-57 1.2435530621024427 1104 58-59 1.3457107381700872 1104 60-61 1.1814397263390397 1104 62-63 1.302749218001889 1104 64-65 1.3318433258355853 1104 66-67 1.3946030704479 1104 68-69 1.472904574217477 1104 70-71 1.5834271522020877 1104 72-73 1.6187853461093553 1104 74-75 1.7549977878876106 1104 76-77 1.5949470117103992 1104 78-79 1.5486002558231178 1104 80-81 1.7568647444890289 1104 82-83 1.7528485292085243 1104 84-85 1.9217736442091713 1104 86-87 1.9434117326361218 1104 88-89 1.9375183416251822 1104 90-91 2.104386808473585 1104 92-93 2.3549735801633283 1104 94-95 2.48059461374989 1104 96-97 2.557047557586163 1104 98-99 2.5952521939095607 1104 100-101 2.1217868948856875 1105 1 0.4607718874618918 1105 2 0.3547200855008157 1105 3 0.31920412368056006 1105 4 0.2836082896540759 1105 5 0.31803411344368726 1105 6 0.44013835839715654 1105 7 0.40797225257370684 1105 8 0.406220406947547 1105 9 0.47985369357926544 1105 10-11 0.5663947923272303 1105 12-13 0.5579889690955255 1105 14-15 0.7407129344810315 1105 16-17 0.552849202389595 1105 18-19 0.6744430285020186 1105 20-21 0.7856879010971838 1105 22-23 0.7506609249891838 1105 24-25 0.7985253038079563 1105 26-27 0.8719500500580253 1105 28-29 0.7708235689876162 1105 30-31 0.7521920881401769 1105 32-33 0.9600800837802552 1105 34-35 0.9847167338447491 1105 36-37 1.0816748205152322 1105 38-39 1.1013282047430337 1105 40-41 1.1854912554399775 1105 42-43 1.1324998733969949 1105 44-45 1.2570601467520675 1105 46-47 1.3885946363915593 1105 48-49 1.3410133935427169 1105 50-51 1.2183690683792499 1105 52-53 1.1145093143122153 1105 54-55 1.0475898742907077 1105 56-57 1.1257762117052152 1105 58-59 1.3957014835253752 1105 60-61 1.23817515329144 1105 62-63 1.316161267549301 1105 64-65 1.5559462719741255 1105 66-67 1.588591776332418 1105 68-69 1.5010578069913372 1105 70-71 1.4554038006066392 1105 72-73 1.680981588263748 1105 74-75 1.6625844122243336 1105 76-77 1.710261896685509 1105 78-79 1.6764520228566937 1105 80-81 1.6765081302634322 1105 82-83 1.6156520140133743 1105 84-85 1.7889776838328508 1105 86-87 1.8422926678955704 1105 88-89 1.828129734495196 1105 90-91 1.7296258628443368 1105 92-93 1.7471684657362552 1105 94-95 1.988654202165371 1105 96-97 2.36902881606839 1105 98-99 2.4769825767931817 1105 100-101 1.9448740144321288 1106 1 0.3539848543207995 1106 2 0.28272107987637796 1106 3 0.27154548340515916 1106 4 0.3073893187487968 1106 5 0.47956471274812884 1106 6 0.12941615115215654 1106 7 0.5312865802322122 1106 8 0.46425616247560697 1106 9 0.2639373017516178 1106 10-11 0.3676742110206419 1106 12-13 0.357924217105726 1106 14-15 0.6169383721868442 1106 16-17 0.4180294287612014 1106 18-19 0.24903715278384908 1106 20-21 0.49969826645462234 1106 22-23 0.529946503100156 1106 24-25 0.40313336110549614 1106 26-27 0.2812804674522056 1106 28-29 0.5156440396867126 1106 30-31 0.17944100201163593 1106 32-33 0.46888920986354776 1106 34-35 0.3377032619035205 1106 36-37 0.3834094347789829 1106 38-39 0.5459518159556325 1106 40-41 0.5465054345300402 1106 42-43 -0.009789894696815793 1106 44-45 0.5005624819389354 1106 46-47 0.6610711774427216 1106 48-49 0.9933980892293661 1106 50-51 0.5070168099961343 1106 52-53 0.19914755561121922 1106 54-55 0.43571464490825207 1106 56-57 -0.10750563917709144 1106 58-59 0.04066657540013452 1106 60-61 0.8646367971857316 1106 62-63 0.664088154070388 1106 64-65 0.6042826665396639 1106 66-67 0.47543662435000655 1106 68-69 0.349865690105176 1106 70-71 0.23227272695817902 1106 72-73 0.7264322780698009 1106 74-75 0.8479930426315647 1106 76-77 0.856803376171893 1106 78-79 1.0646637173515963 1106 80-81 1.2020288966716777 1106 82-83 0.9848576137587308 1106 84-85 0.8204029750698325 1106 86-87 0.8180467850219273 1106 88-89 1.1948960066870313 1106 90-91 1.3309975804181597 1106 92-93 1.1396998990060148 1106 94-95 0.9965562964849148 1106 96-97 0.7548839076659988 1106 98-99 1.01803164301238 1106 100-101 0.870132917410805 1107 1 0.20741272561271273 1107 2 0.1701270781523938 1107 3 0.2038377316620945 1107 4 0.061843680967037074 1107 5 0.3190860996047675 1107 6 0.38949167119260153 1107 7 0.3461821463687471 1107 8 0.26590050812613697 1107 9 0.4831671430127926 1107 10-11 0.5097814670446184 1107 12-13 0.5578970103965943 1107 14-15 0.6549274803985909 1107 16-17 0.7566408904443307 1107 18-19 0.6511750752677585 1107 20-21 0.724627591939786 1107 22-23 0.6324175203231235 1107 24-25 0.7244133992805715 1107 26-27 0.6849904075144337 1107 28-29 0.7269499269132993 1107 30-31 0.9610679310837966 1107 32-33 0.8832262351151812 1107 34-35 0.5220790593934908 1107 36-37 0.7015925675297865 1107 38-39 0.8063636709042399 1107 40-41 0.7454455243389617 1107 42-43 0.8326908912343143 1107 44-45 0.6634905022518538 1107 46-47 0.6857111906390472 1107 48-49 0.8685278633574427 1107 50-51 0.7980951248832646 1107 52-53 0.8486963553695688 1107 54-55 0.578104947108919 1107 56-57 0.8414108188200089 1107 58-59 0.8532368515883952 1107 60-61 0.7268522213011046 1107 62-63 0.7378160596652528 1107 64-65 0.7596580133515651 1107 66-67 0.7507986463012131 1107 68-69 0.7407208617765662 1107 70-71 0.917350650347192 1107 72-73 0.927922059620812 1107 74-75 0.9178915235813818 1107 76-77 0.993229357186646 1107 78-79 0.9251660024741604 1107 80-81 1.0500502378713925 1107 82-83 0.7909574597034172 1107 84-85 0.8443289460427366 1107 86-87 1.201453048938255 1107 88-89 1.1392663199899609 1107 90-91 1.0934623904411538 1107 92-93 0.914947979473606 1107 94-95 0.9253758064803037 1107 96-97 1.1430446511161065 1107 98-99 1.2011256992301167 1107 100-101 1.1285639846876965 1108 1 0.3525122729419188 1108 2 0.35299697784618544 1108 3 0.22759315002023683 1108 4 0.2967529666236359 1108 5 0.2943805697295261 1108 6 0.4003429863684218 1108 7 0.37158184686792595 1108 8 0.563397469321238 1108 9 0.4838584201631093 1108 10-11 0.30807452804771884 1108 12-13 0.4252995049391117 1108 14-15 0.5962354725146568 1108 16-17 0.707658985439167 1108 18-19 0.8408272771091134 1108 20-21 0.6500422595888224 1108 22-23 0.5516905271867145 1108 24-25 0.7988661579314709 1108 26-27 0.9145162539935612 1108 28-29 0.7545703684100218 1108 30-31 0.5980949500773463 1108 32-33 0.7368943310701255 1108 34-35 0.5185189031564335 1108 36-37 0.7368500695834967 1108 38-39 0.664651723567566 1108 40-41 0.7479114214644014 1108 42-43 0.8677091556288801 1108 44-45 0.8332626990743464 1108 46-47 0.9091073447917495 1108 48-49 0.8756830290364306 1108 50-51 0.9246859106428538 1108 52-53 0.7550657851369351 1108 54-55 0.8352906915431291 1108 56-57 1.0037633601710354 1108 58-59 0.9275099518322669 1108 60-61 0.873458216210171 1108 62-63 1.0029507021989446 1108 64-65 1.0053735022999426 1108 66-67 1.080494050334032 1108 68-69 1.0124569643570425 1108 70-71 1.2681188281348383 1108 72-73 1.378931211151766 1108 74-75 1.3366039856933156 1108 76-77 1.30659491680154 1108 78-79 1.5433136322326426 1108 80-81 1.5072026284461373 1108 82-83 1.4745495441065692 1108 84-85 1.499685068198879 1108 86-87 1.4963838936036993 1108 88-89 1.512455367192775 1108 90-91 1.4714204082433824 1108 92-93 1.5098102488144356 1108 94-95 1.5380629254459954 1108 96-97 1.771767006277365 1108 98-99 1.9518334823321783 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-0.34514289698405065 2312 42-43 -0.4130748844742911 2312 44-45 -0.49786534036397967 2312 46-47 -0.6619539851860594 2312 48-49 -0.8334836839420916 2312 50-51 -0.7164223922988633 2312 52-53 -0.747279931390679 2312 54-55 -0.5791098385023901 2312 56-57 -0.7136602036049737 2312 58-59 -0.8687712659671867 2312 60-61 -1.0367052892689905 2312 62-63 -0.9450750363136358 2312 64-65 -1.060149525057362 2312 66-67 -0.8951217400869069 2312 68-69 -1.104261393134081 2312 70-71 -0.8781170890259204 2312 72-73 -1.1105669179344062 2312 74-75 -1.3521516921821757 2312 76-77 -1.5124398660332652 2312 78-79 -1.650128507609434 2312 80-81 -1.7797435221722395 2312 82-83 -1.7025779926144793 2312 84-85 -1.5036789567208082 2312 86-87 -1.3340422026233227 2312 88-89 -1.1573914366011628 2312 90-91 -1.13570030661446 2312 92-93 -1.4400920119347127 2312 94-95 -1.519326253692153 2312 96-97 -1.6964033679716835 2312 98-99 -1.5696611226345265 2312 100-101 -1.2963575345552467 2313 1 -0.4308614272897522 2313 2 -0.4590009666136545 2313 3 -0.37140034410783684 2313 4 -0.4671912050699518 2313 5 -0.3259639361305915 2313 6 -0.2981780468358437 2313 7 -0.24266203973631661 2313 8 -0.1694090184196142 2313 9 -0.4653702001261806 2313 10-11 -0.4336801338346703 2313 12-13 -0.44053322637913794 2313 14-15 -0.6600115949891858 2313 16-17 -0.6311453445494948 2313 18-19 -0.3818570107723147 2313 20-21 -0.4318941547776589 2313 22-23 -0.49433107550284916 2313 24-25 -0.4384409971408516 2313 26-27 -0.38964102718733074 2313 28-29 -0.36392520424307406 2313 30-31 -0.5995021943551677 2313 32-33 -0.5039437612427307 2313 34-35 -0.48679021500296926 2313 36-37 -0.24450599625029668 2313 38-39 -0.2702913548683483 2313 40-41 -0.5055400960116714 2313 42-43 -0.5477249103009498 2313 44-45 -0.518759082871469 2313 46-47 -0.7067739885974049 2313 48-49 -0.7223552449980986 2313 50-51 -0.6248220720386364 2313 52-53 -0.6016933183455357 2313 54-55 -0.4207091923768971 2313 56-57 -0.5163729208062051 2313 58-59 -0.7480728300141628 2313 60-61 -0.6981707069143219 2313 62-63 -0.8112928248655393 2313 64-65 -0.5681487828731271 2313 66-67 -0.6365204239661928 2313 68-69 -0.6515374658584712 2313 70-71 -0.6274380822578358 2313 72-73 -0.7306752546057993 2313 74-75 -0.46626408863998847 2313 76-77 -0.6433136188520905 2313 78-79 -0.7827185614709435 2313 80-81 -1.0644991014794059 2313 82-83 -1.0314117534929608 2313 84-85 -0.944547747148949 2313 86-87 -1.0618542280373333 2313 88-89 -1.1077184856892082 2313 90-91 -0.9486054940280617 2313 92-93 -1.0651743359184245 2313 94-95 -1.2175707415487942 2313 96-97 -1.170236662569053 2313 98-99 -1.522078826434404 2313 100-101 -1.463456497768325 2314 1 -0.648792744008599 2314 2 -0.5138159690363473 2314 3 -0.4975714152226125 2314 4 -0.3524464045743585 2314 5 -0.5071252944630729 2314 6 -0.4142058244642257 2314 7 -0.58498835499568 2314 8 -0.7434478979072807 2314 9 -0.7503842546908501 2314 10-11 -0.612312086125911 2314 12-13 -0.9068463979680388 2314 14-15 -0.9508690872284902 2314 16-17 -1.130904175162783 2314 18-19 -0.9152405744857717 2314 20-21 -0.9078366222497465 2314 22-23 -0.8243296380208491 2314 24-25 -0.7009730043399998 2314 26-27 -0.6941477532829552 2314 28-29 -0.7550294642258706 2314 30-31 -0.9609180505639401 2314 32-33 -0.9768089660130173 2314 34-35 -0.9905638497251061 2314 36-37 -0.824598068439272 2314 38-39 -1.1101513372763065 2314 40-41 -0.9317634103073331 2314 42-43 -0.6975629735530902 2314 44-45 -0.866380001266009 2314 46-47 -0.910497213661607 2314 48-49 -1.2398207735440963 2314 50-51 -1.2031527398370336 2314 52-53 -1.1446206135373558 2314 54-55 -1.1197317535102194 2314 56-57 -1.2178514248274865 2314 58-59 -1.3908975457080892 2314 60-61 -1.4116134371913596 2314 62-63 -1.539038134347102 2314 64-65 -1.4585356464389712 2314 66-67 -1.596907771582103 2314 68-69 -1.7830154858296936 2314 70-71 -1.577174494997056 2314 72-73 -1.645718234828685 2314 74-75 -1.6732068577877115 2314 76-77 -1.6020086247755927 2314 78-79 -1.9249630282653563 2314 80-81 -1.8624091786682797 2314 82-83 -1.8422577018717128 2314 84-85 -1.879955300187877 2314 86-87 -1.8349044230508333 2314 88-89 -1.9627931638570324 2314 90-91 -1.8753505067233718 2314 92-93 -2.0843769441170608 2314 94-95 -2.0803007447806827 2314 96-97 -2.2608910493583014 2314 98-99 -2.141901507651257 2314 100-101 -1.7475147979311902 2315 1 -0.6947061896643625 2315 2 -0.6778889419101155 2315 3 -0.6958572134093046 2315 4 -0.5357722742167184 2315 5 -0.35988257767955645 2315 6 -0.4421719074947248 2315 7 -0.5487047836807193 2315 8 -0.6487407560115059 2315 9 -0.7011077432934272 2315 10-11 -0.7614144855190261 2315 12-13 -0.7921403572725652 2315 14-15 -1.076137841281522 2315 16-17 -1.1627517445689932 2315 18-19 -1.124915230972455 2315 20-21 -0.9150164482600971 2315 22-23 -0.9899620328868082 2315 24-25 -1.0731778520684614 2315 26-27 -1.0418489364615127 2315 28-29 -1.2824984164949527 2315 30-31 -1.1452456454201112 2315 32-33 -1.4000196990453588 2315 34-35 -1.4117685628624201 2315 36-37 -1.5297872913589217 2315 38-39 -1.6890938179135304 2315 40-41 -1.6459372041871063 2315 42-43 -1.8050223820861682 2315 44-45 -1.703313158407294 2315 46-47 -1.5746602402494823 2315 48-49 -1.5575536468204092 2315 50-51 -1.3914691269211765 2315 52-53 -1.604587944594872 2315 54-55 -1.7964159170262306 2315 56-57 -1.6307853895156654 2315 58-59 -1.730264355530796 2315 60-61 -1.9775920983764976 2315 62-63 -2.0037581235798108 2315 64-65 -1.9952325349363242 2315 66-67 -1.9155944066810235 2315 68-69 -1.8149473582372835 2315 70-71 -1.8457994628787127 2315 72-73 -2.122889985370108 2315 74-75 -2.2050965175168145 2315 76-77 -2.400613648696112 2315 78-79 -2.711064167395893 2315 80-81 -2.543476608833828 2315 82-83 -2.4079041676305017 2315 84-85 -2.33501715093853 2315 86-87 -2.4885500078493763 2315 88-89 -2.7809739809476604 2315 90-91 -2.7256064746172655 2315 92-93 -2.764708038343098 2315 94-95 -3.1158137205909817 2315 96-97 -2.9462246518739335 2315 98-99 -2.9176401071691753 2315 100-101 -2.4707793674230487 2316 1 -0.998014585341835 2316 2 -0.8663474253311918 2316 3 -0.8583214232550596 2316 4 -0.7423250281343883 2316 5 -0.7830899912444096 2316 6 -0.7303292918117137 2316 7 -0.7629124743306406 2316 8 -1.0547528861799123 2316 9 -1.060908242046544 2316 10-11 -1.108135337709541 2316 12-13 -1.3839491070031826 2316 14-15 -1.5182221367157709 2316 16-17 -1.458842382172513 2316 18-19 -1.4517432227214755 2316 20-21 -1.4663326023193903 2316 22-23 -1.546290963646328 2316 24-25 -1.3066521909127147 2316 26-27 -1.4813772761062225 2316 28-29 -1.5942086470913068 2316 30-31 -1.5914899112986234 2316 32-33 -1.813715090197114 2316 34-35 -1.524143181870457 2316 36-37 -1.6548006838037281 2316 38-39 -1.9028723345848206 2316 40-41 -1.7715478688833883 2316 42-43 -1.7970023088125657 2316 44-45 -1.8032688247968736 2316 46-47 -1.989649926527612 2316 48-49 -2.03085619292429 2316 50-51 -1.9672475820180004 2316 52-53 -1.9116573082228427 2316 54-55 -2.133111831560882 2316 56-57 -2.2395837639499483 2316 58-59 -2.015312845417192 2316 60-61 -2.1351340459766313 2316 62-63 -2.3792053080876734 2316 64-65 -2.620662786467989 2316 66-67 -2.781095468840441 2316 68-69 -2.9495201854285646 2316 70-71 -3.228934218582051 2316 72-73 -2.8915645951419755 2316 74-75 -3.107906160077082 2316 76-77 -3.2419959952987405 2316 78-79 -3.2831666272955253 2316 80-81 -3.4107454737086798 2316 82-83 -3.464513262178084 2316 84-85 -3.3516330186765906 2316 86-87 -3.3746464950264965 2316 88-89 -3.6866542247825187 2316 90-91 -3.6467086574318373 2316 92-93 -3.530816840412003 2316 94-95 -3.850669174548681 2316 96-97 -3.960577042502301 2316 98-99 -4.0131648754952725 2316 100-101 -3.3233651868251606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9058.0 3 3443.0 4 3639.0 5 4217.0 6 4905.0 7 5971.0 8 7210.0 9 8908.0 10 11102.0 11 13417.0 12 15853.0 13 18114.0 14 20274.0 15 22753.0 16 25656.0 17 28623.0 18 27780.0 19 26836.0 20 28407.0 21 31100.0 22 33523.0 23 37321.0 24 41295.0 25 45123.0 26 50335.0 27 55775.0 28 62425.0 29 70273.0 30 80088.0 31 90903.0 32 105243.0 33 122269.0 34 142644.0 35 167922.0 36 187053.0 37 168724.0 38 71161.0 39 3146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.238455857583375 18.393969232895408 13.8636599207069 23.503914988814316 2 15.636035862720421 19.59901669872105 40.14804639392251 24.61690104463602 3 19.485164567907905 23.931524617629574 29.119386045506136 27.46392476895639 4 12.931649028595457 15.810867559622736 36.471758818630256 34.78572459315155 5 13.932984127876674 37.29889507183439 33.703444979594835 15.0646758206941 6 32.97995524958273 35.30846826810698 17.146996097171407 14.564580385138884 7 29.4169928513845 32.219904566334066 20.094850502195776 18.26825208008566 8 26.75073083232271 34.86602037367211 19.37588485516723 19.007363938837944 9 26.84951758523183 16.490815248291476 19.86434396289059 36.79532320358611 10-11 25.42289989424802 26.37223724305996 26.869050100945067 21.33581276174695 12-13 26.297781096448645 23.56601687031033 27.38072701396587 22.755475019275146 14-15 22.88606765155886 25.057648834698433 25.727403965181484 26.32887954856123 16-17 22.544415107819074 28.336624141309713 26.464576047363874 22.654384703507336 18-19 22.5903672200677 26.653837648568967 28.87201393893427 21.883781192429062 20-21 23.045424751962116 25.913500763349294 29.038736229532503 22.002338255156083 22-23 22.74788323217487 25.864067866168433 28.829837786465646 22.55821111519105 24-25 22.531790846072624 26.092743666809792 29.040914258555645 22.33455122856194 26-27 22.133524106748855 26.44542459777975 28.795253998166803 22.625797297304594 28-29 22.218978567393187 26.77852504863682 28.776334366381885 22.22616201758811 30-31 22.326784632944886 26.37182119557672 28.720288979028275 22.581105192450117 32-33 21.981102268645913 26.38018519668709 28.68566521472608 22.953047319940914 34-35 22.71267994925784 26.49195533674477 28.570255663457516 22.22510905053987 36-37 22.757389805434713 26.14318278853235 28.43677229080525 22.662655115227683 38-39 22.25916949103577 26.50653643564394 28.54472609987048 22.689567973449805 40-41 22.700012987374166 26.350005143648186 28.43192712382044 22.518054745157208 42-43 22.333742828214646 26.31800202497469 28.468444144448195 22.879811002362473 44-45 22.36227809341185 26.54576118197582 28.466774205390326 22.625186519222005 46-47 22.666995995416393 26.900679038090107 27.790109609785922 22.642215356707577 48-49 22.548571311344425 26.772067366038844 27.81019344306192 22.86916787955481 50-51 22.669987180964878 26.98321864404355 27.709954551675235 22.636839623316337 52-53 22.92594902027474 26.81121629537252 27.614701682498293 22.648133001854447 54-55 22.45600509855781 26.9473680800804 27.7983424288387 22.798284392523087 56-57 22.447761532468753 27.128829241772202 27.626284467292617 22.79712475846643 58-59 22.573307489215118 26.614222543773923 27.68795926851787 23.124510698493086 60-61 22.31211716171127 26.955649140123167 27.87531144735922 22.856922250806342 62-63 22.389709137475677 26.901004381617504 27.91939572981249 22.789890751094326 64-65 22.122879060385184 26.825757888630747 27.86504158133511 23.18632146964896 66-67 22.15361434400431 26.890824125931132 27.745916729021104 23.209644801043453 68-69 22.524355184840264 26.565574766165934 27.926549783374497 22.983520265619305 70-71 22.298371975043015 26.7371489867334 27.63457550367689 23.329903534546695 72-73 22.44509402598272 26.694966391039987 27.585886639652067 23.274052943325227 74-75 22.21205273374749 27.022724756944726 27.578923780133856 23.186298729173924 76-77 22.395483075165362 26.763033417177066 27.59170684047706 23.249776667180512 78-79 22.55893310127365 26.81293480825115 27.500125513051465 23.128006577423736 80-81 22.443487010946843 26.92209483234237 27.373804662692734 23.260613494018052 82-83 22.493087264046537 26.85963664003783 27.334971587872452 23.312304508043173 84-85 22.405465575898003 26.74965477106922 27.44211004786239 23.40276960517039 86-87 22.47959981654749 26.768301587717215 27.277654939221236 23.47444365651406 88-89 22.448949284636562 26.774395097489666 27.23875761022062 23.53789800765315 90-91 22.330050164203456 26.72929339457404 27.214519133489596 23.726137307732913 92-93 22.499577469427955 26.395822320089724 27.388620671221535 23.715979539260783 94-95 22.326213533506483 26.20181389362804 27.558075542356136 23.913897030509336 96-97 22.48026388980397 26.057903030865994 27.463300933961666 23.998532145368372 98-99 22.28810273832806 26.065817281369497 27.193459416438177 24.452620563864265 100-101 22.868904156025515 25.557294754626625 27.441815237634763 24.131985851713093 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 329.0 1 233.5 2 215.5 3 278.5 4 236.0 5 265.5 6 441.5 7 800.5 8 1292.0 9 1557.0 10 1459.0 11 1320.0 12 1369.5 13 1467.0 14 1566.5 15 1742.0 16 1917.0 17 2123.5 18 2332.5 19 2558.0 20 2858.0 21 3331.0 22 3986.5 23 4748.0 24 5735.0 25 7149.0 26 9216.5 27 11767.0 28 14103.5 29 16667.0 30 19724.0 31 23482.5 32 28679.5 33 32112.0 34 33785.5 35 36707.0 36 40526.0 37 44421.5 38 47445.0 39 49658.0 40 52463.5 41 56439.0 42 59565.0 43 62481.0 44 65187.5 45 67349.0 46 68782.5 47 69813.0 48 70955.5 49 70166.0 50 68356.5 51 65854.5 52 63639.0 53 62809.0 54 62310.0 55 59271.0 56 54472.5 57 50668.5 58 47484.0 59 42578.5 60 35573.5 61 29838.5 62 25187.5 63 19523.0 64 14652.5 65 11553.5 66 8719.5 67 6630.0 68 5163.5 69 3845.5 70 2979.5 71 2252.5 72 1669.5 73 1393.5 74 1119.5 75 839.5 76 559.5 77 338.5 78 213.0 79 122.0 80 76.5 81 50.0 82 31.0 83 19.0 84 13.5 85 11.5 86 9.5 87 5.0 88 3.0 89 2.0 90 2.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0065317526851711395 2 0.10731507717454733 3 0.11816534403173244 4 0.1203785825448896 5 0.15962308008306664 6 0.09608694032731098 7 0.028178304972391197 8 0.026504880730735782 9 0.02439960507187897 10-11 0.05327966859722244 12-13 0.05460221356240172 14-15 0.029338905656119955 16-17 0.033117605556632186 18-19 0.03765204543724686 20-21 0.05983841199596867 22-23 0.0328746891344564 24-25 0.05041865295826318 26-27 0.05819197846788834 28-29 0.054575222848826634 30-31 0.06920418960652398 32-33 0.05095846722976493 34-35 0.06307729762497916 36-37 0.054116380718050146 38-39 0.032415847003679915 40-41 0.037274175447195634 42-43 0.03314459627020727 44-45 0.007179529810973236 46-47 0.012766607521016318 48-49 0.008691009771178128 50-51 0.008933926193353914 52-53 0.009932582595632147 54-55 0.01082327614361003 56-57 0.014143133913345774 58-59 0.01937933234691272 60-61 0.02431863293115371 62-63 0.011066192565785817 64-65 0.008394111921852168 66-67 0.009446749751280575 68-69 0.011552025410137388 70-71 0.03144418131497677 72-73 0.028475202821717158 74-75 0.029365896369695044 76-77 0.023346967242450563 78-79 0.004966291297816073 80-81 0.004480458453464501 82-83 0.005991938413669393 84-85 0.0051282355792665974 86-87 0.012874570375316668 88-89 0.03794894328657282 90-91 0.036194546904192146 92-93 0.02979774778689644 94-95 0.048151433017955846 96-97 0.043563011710190995 98-99 0.026073029313534384 100-101 0.07457534160796636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1852489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.339101766561136 #Duplication Level Percentage of deduplicated Percentage of total 1 81.3737211827114 49.91390964750107 2 10.969186388770511 13.456800803943434 3 3.24183371921404 5.965535052394181 4 1.4387216445925204 3.5299957348565925 5 0.7710810486715144 2.3648709457364347 6 0.4505064274089731 1.6580195758397336 7 0.30112322735458885 1.2929439800884917 8 0.21618304284365814 1.0608378932153595 9 0.17233213378388276 0.9513628462636391 >10 0.9604330795691661 10.673994455293291 >50 0.06759371245238409 2.871299016772657 >100 0.03339779420354818 3.7189728207309347 >500 0.002738285253096883 1.133539987500196 >1k 0.0010599813882955673 1.1034690208608093 >5k 8.833178235796394E-5 0.3044482190030878 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5619 0.3033216391568317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4349 0.2347652266761098 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2860 0.15438688164949968 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 5.398142715017471E-5 12-13 0.0 0.0 0.0 0.0 2.6990713575087354E-4 14-15 0.0 0.0 0.0 0.0 2.6990713575087354E-4 16-17 0.0 0.0 0.0 0.0 2.6990713575087354E-4 18-19 0.0 0.0 0.0 0.0 3.2388856290104824E-4 20-21 0.0 0.0 0.0 8.097214072526207E-5 3.2388856290104824E-4 22-23 0.0 0.0 0.0 1.6194428145052412E-4 3.2388856290104824E-4 24-25 0.0 0.0 0.0 4.0486070362631037E-4 3.2388856290104824E-4 26-27 0.0 0.0 0.0 0.0012955542516041932 3.2388856290104824E-4 28-29 0.0 0.0 0.0 0.0033198577697357446 3.2388856290104824E-4 30-31 0.0 0.0 0.0 0.011740960405163 3.2388856290104824E-4 32-33 0.0 0.0 0.0 0.03093135775705011 4.318514172013977E-4 34-35 0.0 0.0 0.0 0.061727761946224785 4.318514172013977E-4 36-37 0.0 0.0 0.0 0.11573617980997458 4.318514172013977E-4 38-39 0.0 0.0 0.0 0.19576364556010858 4.318514172013977E-4 40-41 0.0 0.0 0.0 0.2814051797338608 4.318514172013977E-4 42-43 0.0 0.0 0.0 0.3697997666922719 4.318514172013977E-4 44-45 0.0 0.0 0.0 0.47533345677086347 4.318514172013977E-4 46-47 0.0 0.0 0.0 0.6106109132092012 4.318514172013977E-4 48-49 0.0 0.0 0.0 0.751745354493333 5.398142715017471E-4 50-51 0.0 0.0 0.0 0.9042428861925766 5.398142715017471E-4 52-53 0.0 0.0 0.0 1.051747135880429 5.398142715017471E-4 54-55 0.0 0.0 0.0 1.2172272008092895 5.937956986519218E-4 56-57 0.0 0.0 0.0 1.4128289020879476 5.937956986519218E-4 58-59 5.398142715017471E-5 0.0 0.0 1.6232754958329036 5.937956986519218E-4 60-61 5.398142715017471E-5 0.0 0.0 1.8543969761763766 5.937956986519218E-4 62-63 5.398142715017471E-5 0.0 0.0 2.078176982427426 6.477771258020965E-4 64-65 5.398142715017471E-5 0.0 0.0 2.3109988777261297 7.017585529522712E-4 66-67 5.398142715017471E-5 0.0 0.0 2.571648198720748 7.017585529522712E-4 68-69 5.398142715017471E-5 0.0 0.0 2.8736742836259754 7.017585529522712E-4 70-71 8.097214072526207E-5 0.0 0.0 3.1957814594310685 7.287492665273587E-4 72-73 1.0796285430034942E-4 0.0 0.0 3.5277132549774923 7.827306936775333E-4 74-75 1.0796285430034942E-4 0.0 0.0 3.8491186722296327 8.097214072526206E-4 76-77 1.0796285430034942E-4 0.0 0.0 4.199593088002142 8.097214072526206E-4 78-79 1.6194428145052412E-4 0.0 0.0 4.596923382541003 8.36712120827708E-4 80-81 1.6194428145052412E-4 0.0 0.0 5.018518328583868 8.637028344027954E-4 82-83 1.6194428145052412E-4 0.0 0.0 5.449425070810137 8.637028344027954E-4 84-85 1.6194428145052412E-4 0.0 0.0 5.871775756833104 8.637028344027954E-4 86-87 1.6194428145052412E-4 0.0 0.0 6.312210220951379 8.637028344027954E-4 88-89 1.6194428145052412E-4 0.0 0.0 6.786248123470639 8.637028344027954E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3370 0.0 47.558117 1 GTATCAA 6310 0.0 44.844814 1 ATCAACG 8040 0.0 34.838573 3 TCAACGC 8200 0.0 34.042812 4 CAACGCA 8370 0.0 33.237743 5 TATCAAC 8535 0.0 33.04093 2 AACGCAG 8610 0.0 32.510212 6 ACGCAGA 9830 0.0 28.232119 7 CGCAGAG 9960 0.0 27.672781 8 GTACATG 7380 0.0 26.29229 1 TACATGG 7420 0.0 25.700502 2 GCAGAGT 10575 0.0 24.939344 9 ACATGGG 7590 0.0 23.997065 3 GAGTACT 5995 0.0 21.676725 12-13 AGTACTT 6285 0.0 20.978924 12-13 AGAGTAC 9900 0.0 20.73553 10-11 ATAACGC 115 0.0036989935 20.676323 3 GTACTTT 6425 0.0 20.07293 14-15 GGACCGA 690 0.0 19.97361 6 CAGAGTA 10555 0.0 19.606339 10-11 >>END_MODULE