FastQCFastQC Report
Thu 26 May 2016
SRR938447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1852489
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55840.30143228920657555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46880.25306493048001905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32170.17365825114211206No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.046.9024961
GTATCAA65850.037.4567031
ATCAACG81150.030.145713
TCAACGC82600.029.6165184
CAACGCA84450.028.9114785
TATCAAC86950.028.203182
AACGCAG87200.028.0397936
TAAGGTG10850.027.1436825
TGGTATC20050.024.4141062
ACGCAGA100800.024.3037437
CGCAGAG101500.024.089368
GTACATG79100.023.9124761
TACATGG77950.023.5336442
GTAAGGT10050.023.1599064
GTGGTAT22050.022.4152051
ACATGGG79400.022.1952613
GAGTACT60750.021.7261412-13
GCAGAGT112750.021.3910069
GTACTTT62700.020.74756214-15
CATGGGG41000.020.1591514
Produced by FastQC (version 0.11.5)