##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938447_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1852489 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.781548500422943 31.0 31.0 34.0 30.0 34.0 2 32.00836820083682 31.0 31.0 34.0 30.0 34.0 3 32.07066546683948 33.0 31.0 34.0 30.0 34.0 4 35.749231979245224 37.0 35.0 37.0 33.0 37.0 5 35.64586780272379 37.0 35.0 37.0 33.0 37.0 6 35.64516982287074 37.0 35.0 37.0 33.0 37.0 7 35.5816315238579 37.0 35.0 37.0 33.0 37.0 8 35.55656200927509 37.0 35.0 37.0 33.0 37.0 9 37.134056396556204 39.0 37.0 39.0 33.0 39.0 10-11 37.034806144597894 39.0 37.0 39.0 33.0 39.0 12-13 36.925715078470105 39.0 37.0 39.0 33.0 39.0 14-15 37.96924840039536 40.0 37.0 41.0 33.0 41.0 16-17 37.885207685443746 40.0 37.0 41.0 33.0 41.0 18-19 38.12000260190479 40.0 37.0 41.0 33.0 41.0 20-21 38.085869335796325 40.0 37.0 41.0 33.0 41.0 22-23 37.98300178840468 40.0 37.0 41.0 33.0 41.0 24-25 37.86661351295473 40.0 37.0 41.0 32.0 41.0 26-27 37.67755813934657 40.0 37.0 41.0 32.0 41.0 28-29 37.55957660207429 39.5 36.5 41.0 32.0 41.0 30-31 37.311487679548975 39.0 36.0 41.0 31.0 41.0 32-33 37.116739424633565 39.0 36.0 41.0 31.0 41.0 34-35 37.01132692285892 39.0 36.0 41.0 31.0 41.0 36-37 36.965708838217125 39.0 36.0 41.0 31.0 41.0 38-39 36.807225845875465 39.0 35.0 41.0 30.0 41.0 40-41 36.63395922998733 39.0 35.0 41.0 30.0 41.0 42-43 36.42109642756313 39.0 35.0 40.0 30.0 41.0 44-45 36.12447469323705 39.0 35.0 40.0 29.5 41.0 46-47 35.73749749661131 38.0 35.0 40.0 28.5 41.0 48-49 35.384713215571054 38.0 34.0 40.0 27.0 41.0 50-51 35.01320250754525 38.0 34.0 40.0 26.0 41.0 52-53 34.5304787234904 38.0 33.0 40.0 24.5 41.0 54-55 34.20121873868077 38.0 33.0 40.0 24.0 41.0 56-57 33.880356104678626 37.0 33.0 40.0 23.0 41.0 58-59 33.41759816117667 37.0 32.0 39.5 22.5 41.0 60-61 33.030641207586115 36.0 31.5 39.0 22.0 40.5 62-63 32.55020758557811 36.0 31.0 39.0 20.5 40.0 64-65 32.02292510238927 35.0 31.0 38.5 18.5 40.0 66-67 31.52576344582883 35.0 30.0 38.0 17.5 40.0 68-69 31.043394049843208 34.5 29.0 38.0 13.0 40.0 70-71 30.487575094912845 34.0 29.0 37.0 9.5 39.0 72-73 29.996159221458264 34.0 28.5 36.5 7.5 39.0 74-75 29.395834739099666 34.0 28.0 36.0 7.0 39.0 76-77 28.18009364697982 31.5 26.5 34.5 3.5 37.0 78-79 28.862909037516552 33.0 28.0 35.0 2.0 37.0 80-81 28.4817607553945 33.0 27.0 35.0 2.0 37.0 82-83 28.032508964965515 33.0 26.5 35.0 2.0 36.0 84-85 27.479017149359592 33.0 26.0 35.0 2.0 36.0 86-87 26.869110153960428 32.0 25.0 35.0 2.0 35.5 88-89 26.126786717761888 32.0 24.0 34.0 2.0 35.0 90-91 25.52607140987072 31.5 21.5 34.0 2.0 35.0 92-93 24.70319283947165 31.0 18.5 34.0 2.0 35.0 94-95 23.897304653360965 31.0 11.5 34.0 2.0 35.0 96-97 22.850024750484348 30.0 2.0 34.0 2.0 35.0 98-99 21.50251283543384 29.5 2.0 34.0 2.0 35.0 100-101 17.60172449067174 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27343534428985805 1101 2 0.29620440364941913 1101 3 0.26089446148779416 1101 4 0.24906069459460412 1101 5 -0.05870058853444249 1101 6 0.1916487498135524 1101 7 0.20197555909438591 1101 8 0.24670186006120076 1101 9 0.32613736485837563 1101 10-11 0.31308616257375377 1101 12-13 0.32732306194894534 1101 14-15 0.4610349281927313 1101 16-17 0.4117999400414405 1101 18-19 0.485308238808706 1101 20-21 0.42423208197626394 1101 22-23 0.41820751850636384 1101 24-25 0.47824902014078674 1101 26-27 0.42569443020173026 1101 28-29 0.2962487851080766 1101 30-31 0.5166633503699956 1101 32-33 0.40026698023593354 1101 34-35 0.3784773191175077 1101 36-37 0.5468568772004758 1101 38-39 0.75385982407375 1101 40-41 0.7617116982020136 1101 42-43 0.7788928504657164 1101 44-45 0.7452440495398918 1101 46-47 0.6273505093243514 1101 48-49 0.6234301132441402 1101 50-51 0.5611208949646098 1101 52-53 0.7063609435864322 1101 54-55 0.8121090010501746 1101 56-57 0.6862123518500525 1101 58-59 0.8239631357990902 1101 60-61 0.7248430046295979 1101 62-63 0.6557179907021293 1101 64-65 0.5868690164535622 1101 66-67 0.6477249685426791 1101 68-69 0.719293995414791 1101 70-71 0.8271366828714903 1101 72-73 0.7812321013302999 1101 74-75 0.7819682160232375 1101 76-77 0.6501172928792052 1101 78-79 0.7598954922136016 1101 80-81 0.6677936387877033 1101 82-83 0.6544553137856539 1101 84-85 0.7322552334724257 1101 86-87 0.6398692097824359 1101 88-89 0.9664735628294281 1101 90-91 1.2454137001632333 1101 92-93 1.3670387411456986 1101 94-95 1.091866514237914 1101 96-97 1.1750890519360944 1101 98-99 1.3621412504725008 1101 100-101 1.6894642190436038 1102 1 0.28983783033638844 1102 2 0.28147595231286715 1102 3 0.2853693452406887 1102 4 0.16789768904375535 1102 5 0.14453715111785215 1102 6 0.1636177353141619 1102 7 0.17536279749067063 1102 8 0.21754655087061536 1102 9 0.3161217888911736 1102 10-11 0.3191361660096277 1102 12-13 0.3044477689451526 1102 14-15 0.5526983498662261 1102 16-17 0.5031417256275645 1102 18-19 0.4187228878531428 1102 20-21 0.4325478541701102 1102 22-23 0.41792024117015814 1102 24-25 0.34366770061203766 1102 26-27 0.27017813270075663 1102 28-29 0.3053462188911169 1102 30-31 0.30902769056744717 1102 32-33 0.37073563360194584 1102 34-35 0.34017781006589587 1102 36-37 0.49081679962605307 1102 38-39 0.4618820198270086 1102 40-41 0.44392569977487994 1102 42-43 0.5186548970256339 1102 44-45 0.5620985440463073 1102 46-47 0.4840287052608545 1102 48-49 0.3518451822288782 1102 50-51 0.6544278102359229 1102 52-53 0.46381488377166136 1102 54-55 0.30870080381567533 1102 56-57 0.5429630829279191 1102 58-59 0.7366785460807534 1102 60-61 0.5728370262068907 1102 62-63 0.563492421683037 1102 64-65 0.6054504199703743 1102 66-67 0.30667141470251735 1102 68-69 0.394175266841291 1102 70-71 0.5876571415989957 1102 72-73 0.6515220891902054 1102 74-75 0.6660532424794781 1102 76-77 0.5533659128027004 1102 78-79 0.8455079560414731 1102 80-81 0.6587353358046002 1102 82-83 0.6797319017326053 1102 84-85 0.4545553991579574 1102 86-87 0.627035261809219 1102 88-89 0.6210039550536592 1102 90-91 0.7577475374249154 1102 92-93 1.1163859248733985 1102 94-95 1.0387870157229777 1102 96-97 0.9041149431639219 1102 98-99 1.0482167939134115 1102 100-101 1.7027209705382056 1103 1 0.18775005956920054 1103 2 0.23872983990071361 1103 3 0.2179099991372837 1103 4 0.15743600107131783 1103 5 0.026016109004395105 1103 6 0.2509041143559543 1103 7 0.19157625221636465 1103 8 0.200680344296579 1103 9 0.18589566395982615 1103 10-11 0.3018554437445857 1103 12-13 0.3660786255967068 1103 14-15 0.44660273146300966 1103 16-17 0.5058792341370832 1103 18-19 0.4763023597817764 1103 20-21 0.5074314914105784 1103 22-23 0.43500885223357244 1103 24-25 0.40503398573549987 1103 26-27 0.4308298311342753 1103 28-29 0.5108564918168952 1103 30-31 0.4376313739876707 1103 32-33 0.5009136546190973 1103 34-35 0.5790510398114037 1103 36-37 0.4974053613677327 1103 38-39 0.4762795517917553 1103 40-41 0.5008895633838435 1103 42-43 0.4965823551052111 1103 44-45 0.5667615791719101 1103 46-47 0.5427925077352214 1103 48-49 0.5375579543661146 1103 50-51 0.6385803106043184 1103 52-53 0.514977963347711 1103 54-55 0.5724534997660484 1103 56-57 0.5420688981431354 1103 58-59 0.8108416902012934 1103 60-61 0.7100426332965597 1103 62-63 0.6778456249774862 1103 64-65 0.7158140429379785 1103 66-67 0.5891240506143482 1103 68-69 0.5109348836662129 1103 70-71 0.5853300386359166 1103 72-73 0.6183705764743337 1103 74-75 0.6549455006720599 1103 76-77 0.5372643453457897 1103 78-79 0.8193208549538369 1103 80-81 0.9431428782547862 1103 82-83 0.803554725882961 1103 84-85 0.8113578101992083 1103 86-87 0.9169546603519763 1103 88-89 0.7027687354379815 1103 90-91 0.8367343167643462 1103 92-93 1.0468389297848688 1103 94-95 1.2131224510146907 1103 96-97 1.2831704380314193 1103 98-99 1.4346787692915157 1103 100-101 1.7647689307495718 1104 1 0.33031259499888677 1104 2 0.3882109745746831 1104 3 0.3047988389272902 1104 4 0.23963820068831154 1104 5 0.17526978288129413 1104 6 0.19492121419524722 1104 7 0.2466493091887756 1104 8 0.2644679930568259 1104 9 0.23033344780454712 1104 10-11 0.3081966538952514 1104 12-13 0.28511990795433917 1104 14-15 0.43195505579841864 1104 16-17 0.4849869006914034 1104 18-19 0.3677099689377812 1104 20-21 0.33762504633258317 1104 22-23 0.337665404792709 1104 24-25 0.4946525031768303 1104 26-27 0.2884972739714229 1104 28-29 0.49095855892612406 1104 30-31 0.4342330859855821 1104 32-33 0.5266876581009043 1104 34-35 0.641292665966553 1104 36-37 0.6217394906164131 1104 38-39 0.553923206020535 1104 40-41 0.6039113859964047 1104 42-43 0.675348475056424 1104 44-45 0.6414694194020285 1104 46-47 0.5499883248259394 1104 48-49 0.6001709813539833 1104 50-51 0.7103160556539336 1104 52-53 0.5418925287169287 1104 54-55 0.6688825133384739 1104 56-57 0.636513849372335 1104 58-59 0.7201331083542115 1104 60-61 0.6266758817862268 1104 62-63 0.6292953321513295 1104 64-65 0.6223765238718499 1104 66-67 0.5164355173040711 1104 68-69 0.7222866879219794 1104 70-71 0.5366007092839347 1104 72-73 0.5734207096632922 1104 74-75 0.932394806235429 1104 76-77 0.9947071114112447 1104 78-79 1.036845198886411 1104 80-81 0.7492100396091956 1104 82-83 0.8697357479519887 1104 84-85 0.9287154383991343 1104 86-87 1.1537290589284517 1104 88-89 1.3233656067857993 1104 90-91 1.3849536363603896 1104 92-93 1.6859836298002122 1104 94-95 1.481556549283674 1104 96-97 1.6416262931625845 1104 98-99 1.8998860044403685 1104 100-101 2.076320325931313 1105 1 0.19339012650347698 1105 2 0.20164279802136065 1105 3 0.17633287388915164 1105 4 0.12410689498440064 1105 5 0.07587461127661044 1105 6 0.18687455928945695 1105 7 0.1955697060233419 1105 8 0.13176069907470378 1105 9 0.1456908121737044 1105 10-11 0.20660350417177398 1105 12-13 0.18070150489214143 1105 14-15 0.27008082929415167 1105 16-17 0.15859656277041267 1105 18-19 0.27096817869861667 1105 20-21 0.22648179305480198 1105 22-23 0.3373509768016092 1105 24-25 0.26016064352783275 1105 26-27 0.22427650643372488 1105 28-29 0.3795546235752809 1105 30-31 0.45491664209143323 1105 32-33 0.44616808165323363 1105 34-35 0.4936871184680953 1105 36-37 0.5179881692428339 1105 38-39 0.31843805282743176 1105 40-41 0.3668854206823582 1105 42-43 0.3766875819084774 1105 44-45 0.43946553015195633 1105 46-47 0.46231235005605953 1105 48-49 0.5117437133768377 1105 50-51 0.7316161090549613 1105 52-53 0.5526231742945029 1105 54-55 0.5155308948965498 1105 56-57 0.3997181005307482 1105 58-59 0.36659820770942986 1105 60-61 0.4745576890516361 1105 62-63 0.46327376020681044 1105 64-65 0.5872683681156019 1105 66-67 0.5475575653741949 1105 68-69 0.5983720249433766 1105 70-71 0.4788258592695378 1105 72-73 0.6146199736603641 1105 74-75 0.6434833363966632 1105 76-77 0.6105179246300985 1105 78-79 0.865198125049865 1105 80-81 0.987453910418953 1105 82-83 0.8483937324973105 1105 84-85 0.8458987269250393 1105 86-87 0.6801428783548147 1105 88-89 0.8780433123466906 1105 90-91 1.032781927636826 1105 92-93 1.226387626657079 1105 94-95 1.4462893113582567 1105 96-97 1.3159988886075773 1105 98-99 1.3160048651188774 1105 100-101 1.3809136762678982 1106 1 0.017372214305833467 1106 2 0.13661869869130072 1106 3 0.08705911238643438 1106 4 0.08397661834811032 1106 5 0.049022271848528476 1106 6 -0.0659101542018874 1106 7 0.03800653084107353 1106 8 0.04227277729443557 1106 9 -0.20158057592923484 1106 10-11 0.07888629316007467 1106 12-13 0.06395559892278158 1106 14-15 0.12221407561961684 1106 16-17 0.17890231645921517 1106 18-19 0.10712338863027782 1106 20-21 0.20180758962605694 1106 22-23 0.2782188046369001 1106 24-25 0.32312712450493564 1106 26-27 0.18050550507906138 1106 28-29 0.25907057095736263 1106 30-31 0.1505075438909671 1106 32-33 0.06961048555616145 1106 34-35 0.038513384092340175 1106 36-37 0.13641250389724036 1106 38-39 0.14769016314600236 1106 40-41 0.1994616344462301 1106 42-43 0.27141352813997344 1106 44-45 0.20542110071272646 1106 46-47 -0.6692924998763772 1106 48-49 -0.20879224161423338 1106 50-51 0.1229549635242293 1106 52-53 0.042242699664932104 1106 54-55 0.22710445223313513 1106 56-57 0.17852412758885094 1106 58-59 0.11496534057362595 1106 60-61 0.26653205364649324 1106 62-63 0.06270417785294313 1106 64-65 0.34569851449204947 1106 66-67 0.20991037217558528 1106 68-69 -0.1341468274598796 1106 70-71 -0.0711221963651063 1106 72-73 0.3372933322058209 1106 74-75 0.6152481934509737 1106 76-77 0.29534076368990725 1106 78-79 0.6131667518289703 1106 80-81 0.05181915195395348 1106 82-83 0.45884668568355025 1106 84-85 0.5488596817271478 1106 86-87 0.48641265143730195 1106 88-89 0.5264849393937041 1106 90-91 0.6514430983628792 1106 92-93 0.790822995184925 1106 94-95 1.0480952283967682 1106 96-97 1.2633469544883162 1106 98-99 1.2298800995403987 1106 100-101 1.379275140183573 1107 1 0.2717578902948148 1107 2 0.11289771527587789 1107 3 0.17932466807677372 1107 4 0.14401775510654602 1107 5 0.0015653091937508634 1107 6 0.07085711569857267 1107 7 0.09953359308151022 1107 8 0.06946315168927697 1107 9 0.1960441391166441 1107 10-11 0.15103709330777804 1107 12-13 0.22060036900868596 1107 14-15 0.2566154865124233 1107 16-17 0.3061240749712866 1107 18-19 0.18843998448183896 1107 20-21 0.20813743402027995 1107 22-23 0.22897024269624922 1107 24-25 0.17263104390453066 1107 26-27 0.31297834590133533 1107 28-29 0.21410010335812046 1107 30-31 0.16934457489992383 1107 32-33 0.21916115979249895 1107 34-35 0.15374303576345483 1107 36-37 0.28983102957745643 1107 38-39 0.28385063774241104 1107 40-41 0.29638406292351505 1107 42-43 0.14172750052569683 1107 44-45 0.09098970888337021 1107 46-47 0.13454565448045486 1107 48-49 0.057896302888416074 1107 50-51 0.25744969664275175 1107 52-53 0.3851618456634682 1107 54-55 0.2587025277333552 1107 56-57 0.43027227915271027 1107 58-59 0.18296042301450655 1107 60-61 0.2982747317346224 1107 62-63 0.42371109922903827 1107 64-65 0.2517823470390468 1107 66-67 0.20387067019616367 1107 68-69 0.2949043144587229 1107 70-71 0.48747251284043003 1107 72-73 0.47853457939779176 1107 74-75 0.3955513393873886 1107 76-77 0.18417297193477467 1107 78-79 0.5651438307120884 1107 80-81 0.5686127341697649 1107 82-83 0.7952081043206292 1107 84-85 1.0045723274644622 1107 86-87 0.9668136451248621 1107 88-89 0.8987268123935372 1107 90-91 0.865814426561748 1107 92-93 1.1795878561133861 1107 94-95 1.192771724290452 1107 96-97 1.0295293899333053 1107 98-99 1.0608675236675538 1107 100-101 1.186026099447684 1108 1 0.2135256816722091 1108 2 0.17506057627945637 1108 3 0.17154964143708895 1108 4 0.21905078673826495 1108 5 0.07576791389870152 1108 6 0.1565199802532362 1108 7 0.034282869647348946 1108 8 0.08162915605737453 1108 9 0.2326620998543092 1108 10-11 0.18058116867067042 1108 12-13 0.3315245734152299 1108 14-15 0.4135553909068008 1108 16-17 0.4412727035042536 1108 18-19 0.32466445911251895 1108 20-21 0.3539923094838642 1108 22-23 0.19812726072442643 1108 24-25 0.3245457737632549 1108 26-27 0.3923039771586261 1108 28-29 0.3327817105196189 1108 30-31 0.5213541843888265 1108 32-33 0.4240265882804337 1108 34-35 0.3383478359309606 1108 36-37 0.5024521510404796 1108 38-39 0.5340927031143963 1108 40-41 0.5317303946171208 1108 42-43 0.6600694927980228 1108 44-45 0.7332336608001455 1108 46-47 0.6885377405670852 1108 48-49 0.7384691790985158 1108 50-51 0.8608214088347168 1108 52-53 0.6799407829642661 1108 54-55 0.7259507738280249 1108 56-57 0.6462047181221209 1108 58-59 0.71522515270226 1108 60-61 0.8689913583442106 1108 62-63 1.0137244328815314 1108 64-65 1.0143536349951674 1108 66-67 0.853294776882219 1108 68-69 0.9633178938986475 1108 70-71 0.7389382545074206 1108 72-73 0.869333701570504 1108 74-75 1.002396403608035 1108 76-77 0.9610038239391194 1108 78-79 1.1832034725684508 1108 80-81 1.0435182951366997 1108 82-83 1.2196506963574194 1108 84-85 1.310878337551113 1108 86-87 1.429486125744507 1108 88-89 1.2890218125500503 1108 90-91 1.4383361861409192 1108 92-93 1.6873014621995708 1108 94-95 1.9953074067385614 1108 96-97 2.0594413310095554 1108 98-99 1.9634530790790201 1108 100-101 2.145062009822606 1109 1 0.16313509458391806 1109 2 0.15560621811501107 1109 3 0.1722458382448977 1109 4 0.018913642860162838 1109 5 0.09420513541997622 1109 6 0.20020226305086197 1109 7 0.09911767937744997 1109 8 0.11716690122893425 1109 9 0.10036016715682194 1109 10-11 0.14555902271914078 1109 12-13 0.21782212434651171 1109 14-15 0.4047363006113116 1109 16-17 0.41509239487262306 1109 18-19 0.22591383598191328 1109 20-21 0.35551594653730234 1109 22-23 0.2584833881386501 1109 24-25 0.3307016700125587 1109 26-27 0.3299456980137663 1109 28-29 0.39979058434063575 1109 30-31 0.4068852295277452 1109 32-33 0.47681595248868547 1109 34-35 0.4063315231436633 1109 36-37 0.39827008992295276 1109 38-39 0.41465924464026216 1109 40-41 0.5162813049392483 1109 42-43 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-1.3251183781531495 2316 68-69 -1.2266932832599657 2316 70-71 -0.955237160676635 2316 72-73 -1.2259185374311592 2316 74-75 -1.184253317741895 2316 76-77 -1.144777012880855 2316 78-79 -1.4781669171781644 2316 80-81 -1.2989307618148835 2316 82-83 -1.2978713496467797 2316 84-85 -1.2474163214287195 2316 86-87 -1.43135162890583 2316 88-89 -1.6304560414874238 2316 90-91 -1.4393981609689206 2316 92-93 -1.674279748750152 2316 94-95 -1.8037580477927264 2316 96-97 -2.0587583545760673 2316 98-99 -1.8598095607815068 2316 100-101 -1.963866475834676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 9.0 4 36.0 5 52.0 6 152.0 7 367.0 8 811.0 9 1472.0 10 2436.0 11 3788.0 12 5491.0 13 7583.0 14 9918.0 15 12942.0 16 15847.0 17 19717.0 18 19126.0 19 16405.0 20 16424.0 21 17524.0 22 19200.0 23 21544.0 24 25189.0 25 29515.0 26 35034.0 27 41903.0 28 49876.0 29 59477.0 30 70105.0 31 82989.0 32 99991.0 33 121861.0 34 153307.0 35 197531.0 36 255800.0 37 287234.0 38 145178.0 39 6650.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.782170083164104 19.301510082149246 14.622544240645793 23.29377559404086 2 16.129420781188987 20.381353391905314 39.35018479501337 24.13904103189233 3 19.596931479755074 24.016606846248482 29.604872147688866 26.78158952630758 4 13.369742006565222 16.577642296391502 36.10159088663954 33.95102481040374 5 13.777268686685396 37.377637219671364 34.012667511311435 14.832426582331804 6 32.97309727615117 35.836056246487836 16.82665861983526 14.36418785752574 7 29.357475267059613 32.31679108485934 20.043681770849922 18.28205187723112 8 26.684908790281614 35.46018356924117 19.186456707705148 18.66845093277207 9 26.544934949681213 16.529274937664947 19.785380641936335 37.14040947071751 10-11 25.432485698970414 26.51419252691919 26.85813519000653 21.195186584103872 12-13 26.15688946061218 23.6525561015477 27.50170176449091 22.688852673349206 14-15 22.731767907933598 25.202956670727872 25.721016427088095 26.344258994250435 16-17 22.447717638269378 28.50475765308188 26.536270930623612 22.511253778025132 18-19 22.437920009241623 26.782939061986333 28.980819859119272 21.798321069652776 20-21 22.840621455781925 25.97167918405993 29.201010100464835 21.98668925969331 22-23 22.708043070701095 25.905336010092366 28.951238037040977 22.435382882165563 24-25 22.54080173960738 26.15651715746081 29.061954078338477 22.240727024593333 26-27 22.105194117092942 26.58725478102936 28.930716944546187 22.376834157331515 28-29 22.199157133086494 26.794843904437656 28.90557396624106 22.100424996234793 30-31 22.334250583346716 26.38427602252683 28.849433792678102 22.43203960144835 32-33 21.973855602297675 26.436330521068776 28.808689535817102 22.78112434081645 34-35 22.61078591085839 26.534010157765238 28.697103970652343 22.15809996072403 36-37 22.70633062574106 26.17716551588596 28.583330273703673 22.53317358466931 38-39 22.206848822558335 26.507228976800985 28.6709555032752 22.614966697365478 40-41 22.64940608960587 26.299320690020018 28.619576694442223 22.43169652593189 42-43 22.33329698519155 26.35447211701654 28.51857210103776 22.793658796754144 44-45 22.36472123660304 26.564993056052298 28.55051671338704 22.519768993957616 46-47 22.718083616151027 26.908742777959816 27.87101569833883 22.502157907550327 48-49 22.490740835708067 26.817703101071043 27.901407241824376 22.79014882139651 50-51 22.71793141607849 26.965735030162353 27.80758174874155 22.508751805017614 52-53 22.9562496727376 26.83999743048407 27.61608840862213 22.587664488156207 54-55 22.481799187404196 26.949726539083063 27.836817936812082 22.73165633670066 56-57 22.478783949594302 27.086233710429592 27.691932313768124 22.743050026207985 58-59 22.540029982498062 26.656481724201512 27.660649739576655 23.14283855372377 60-61 22.317298510274554 26.983372100994927 27.882837630884715 22.816491757845796 62-63 22.36065871138984 26.968464031601563 27.91144695538849 22.75943030162011 64-65 22.03916457614541 26.894932781631887 27.855974175707445 23.20992846651526 66-67 22.098647731634465 26.957452861713836 27.69386121231785 23.25003819433385 68-69 22.487933138247904 26.648732844539946 27.785906337397016 23.077427679815138 70-71 22.218725421554915 26.911686829651167 27.49965316895611 23.369934579837807 72-73 22.39648040809992 26.912138408491288 27.354327627042018 23.337053556366776 74-75 22.156266063090417 27.22027005429595 27.319834218108152 23.303629664505486 76-77 22.250843539747972 26.976340380946468 27.505377186148532 23.267438893157035 78-79 22.455684685280605 26.96136862809154 27.42955650261687 23.153390184010984 80-81 22.328209600149027 27.198829375230325 27.276907023613628 23.19605400100702 82-83 22.320852637681096 27.190448894093418 27.22803381454812 23.260664653677367 84-85 22.25610077073023 27.0730364816628 27.20493336453939 23.465929383067582 86-87 22.30108184010534 27.1878527695463 27.074237693859605 23.436827696488756 88-89 22.223200699533237 27.264372833712013 26.888511484787394 23.623914981967353 90-91 22.09974060811307 27.23585115527661 27.07680893984095 23.587599296769373 92-93 22.22014943505601 26.951791590039537 27.11485484670434 23.713204128200115 94-95 21.962714721615818 26.77110741898854 27.314738570543813 23.951439288851837 96-97 22.046187011820003 26.66345713520426 27.23463853079422 24.055717322181515 98-99 21.788524620710383 26.882230037420594 26.9177143866352 24.41153095523382 100-101 21.935636433527623 26.797834823790588 27.199810174557342 24.066718568124443 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 321.0 1 236.0 2 200.5 3 276.0 4 248.5 5 241.0 6 424.5 7 879.0 8 1384.5 9 1591.5 10 1434.5 11 1257.0 12 1298.5 13 1396.5 14 1553.0 15 1718.0 16 1870.0 17 1993.0 18 2190.0 19 2488.5 20 2753.0 21 3155.5 22 3809.0 23 4665.5 24 5682.0 25 7104.0 26 9111.0 27 11624.0 28 14043.5 29 16783.0 30 20044.0 31 23724.0 32 29164.5 33 32569.5 34 34049.5 35 36840.5 36 40792.5 37 45006.0 38 47589.5 39 49743.5 40 52829.0 41 57158.0 42 60333.5 43 63306.5 44 66588.0 45 69009.5 46 69832.0 47 70826.5 48 72264.0 49 71093.0 50 69264.5 51 66732.5 52 64098.5 53 63505.5 54 63646.5 55 60065.0 56 54674.5 57 50515.0 58 47019.5 59 41981.5 60 34291.5 61 28062.5 62 23636.5 63 17990.0 64 13002.0 65 10035.5 66 7371.5 67 5570.5 68 4293.5 69 3113.5 70 2364.5 71 1784.0 72 1372.0 73 1146.5 74 868.0 75 681.0 76 483.0 77 252.5 78 134.5 79 79.0 80 44.0 81 25.5 82 19.0 83 13.0 84 8.5 85 4.5 86 2.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.7786999005122294E-4 2 0.052793835752870874 3 0.0 4 0.0 5 0.044426714544593786 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006315826976570441 26-27 0.0020243035181315514 28-29 1.0796285430034941E-4 30-31 0.0017004149552305033 32-33 4.3185141720139764E-4 34-35 0.011417071842261952 36-37 0.022294329413022156 38-39 0.004480458453464501 40-41 0.0011336099701536688 42-43 0.0017813870959557656 44-45 0.008448093349002341 46-47 0.0 48-49 0.0 50-51 7.557399801024459E-4 52-53 0.0 54-55 0.0016464335280803287 56-57 0.0 58-59 0.02205141299084637 60-61 0.0 62-63 0.009473740464855662 64-65 0.015087808888473832 66-67 0.006477771258020965 68-69 0.016680260989403985 70-71 1.0796285430034941E-4 72-73 8.097214072526207E-5 74-75 0.022510255121622854 76-77 0.04947397798313512 78-79 0.030310571344823102 80-81 0.02626196430856 82-83 0.036842324029994246 84-85 0.015357716024224706 86-87 0.009824619741331798 88-89 0.08315838852484414 90-91 0.02510136362483124 92-93 0.024129697936128097 94-95 0.026882750720787007 96-97 0.03848875755807457 98-99 0.0522810121949442 100-101 0.014089152486195598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1852489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.791202555234186 #Duplication Level Percentage of deduplicated Percentage of total 1 78.94817253275018 45.62509830205742 2 12.232324013203808 14.138414295366331 3 3.739997857072645 6.484169211426812 4 1.6454201866353644 3.803632451772623 5 0.8726010779789214 2.5214332823697774 6 0.5197473593792808 1.8022094954061672 7 0.3554730161750029 1.4380249156482743 8 0.25377951325780085 1.1732978604040247 9 0.20741987676490564 1.0788339700892138 >10 1.0989093227497013 11.59881540450048 >50 0.08193242827741815 3.230219184581393 >100 0.0395489290050178 4.194611048596336 >500 0.0030847652549622654 1.174220174404363 >1k 0.0014956437599817044 1.435362073699113 >5k 9.347773499885652E-5 0.3016583296776758 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5584 0.30143228920657555 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4688 0.25306493048001905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3217 0.17365825114211206 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.3495356787543677E-4 14-15 0.0 0.0 0.0 0.0 2.1592570860069885E-4 16-17 0.0 0.0 0.0 0.0 3.2388856290104824E-4 18-19 0.0 0.0 0.0 0.0 3.2388856290104824E-4 20-21 0.0 0.0 0.0 8.097214072526207E-5 3.2388856290104824E-4 22-23 0.0 0.0 0.0 1.6194428145052412E-4 3.2388856290104824E-4 24-25 0.0 0.0 0.0 4.0486070362631037E-4 3.2388856290104824E-4 26-27 0.0 0.0 0.0 0.0013495356787543678 3.508792764761356E-4 28-29 0.0 0.0 0.0 0.003400829910461007 4.318514172013977E-4 30-31 0.0 0.0 0.0 0.011902904686613524 4.858328443515724E-4 32-33 0.0 0.0 0.0 0.03103932061135046 4.858328443515724E-4 34-35 0.0 0.0 0.0 0.06207864122270092 4.858328443515724E-4 36-37 0.0 0.0 0.0 0.11646492907650194 4.858328443515724E-4 38-39 0.0 0.0 0.0 0.19773396765108997 5.398142715017471E-4 40-41 0.0 0.0 0.0 0.2847250375035965 5.398142715017471E-4 42-43 0.0 0.0 0.0 0.37384837372853497 5.398142715017471E-4 44-45 0.0 0.0 0.0 0.48086655305375636 5.937956986519217E-4 46-47 0.0 0.0 0.0 0.6171966473215225 6.477771258020965E-4 48-49 0.0 0.0 0.0 0.7596806242844087 6.477771258020965E-4 50-51 0.0 0.0 0.0 0.9128799145366046 6.477771258020965E-4 52-53 0.0 0.0 0.0 1.0614907834810354 6.477771258020965E-4 54-55 0.0 0.0 0.0 1.2283473748022256 6.477771258020965E-4 56-57 0.0 0.0 0.0 1.42435393678451 6.747678393771838E-4 58-59 0.0 0.0 0.0 1.635043446951642 7.017585529522712E-4 60-61 0.0 0.0 0.0 1.868000295818221 7.017585529522712E-4 62-63 0.0 0.0 0.0 2.0936696520195266 7.017585529522712E-4 64-65 0.0 0.0 0.0 2.3280300179920097 7.017585529522712E-4 66-67 0.0 0.0 0.0 2.590784614645485 7.017585529522712E-4 68-69 0.0 0.0 0.0 2.895779678043972 7.017585529522712E-4 70-71 0.0 0.0 0.0 3.2224752751568295 7.55739980102446E-4 72-73 0.0 0.0 0.0 3.5615596098006517 8.367121208277081E-4 74-75 0.0 0.0 0.0 3.8900905754366155 9.176842615529701E-4 76-77 0.0 0.0 0.0 4.250605536659057 9.176842615529701E-4 78-79 0.0 0.0 0.0 4.660513503723909 9.176842615529701E-4 80-81 0.0 0.0 0.0 5.094848066574214 9.446749751280575E-4 82-83 0.0 0.0 0.0 5.5410585433975585 0.0010256471158533195 84-85 0.0 0.0 0.0 5.981142128239358 0.0010256471158533195 86-87 0.0 0.0 0.0 6.440739998995945 0.0011066192565785816 88-89 0.0 0.0 0.0 6.9395823672907095 0.0011875913973038437 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3820 0.0 46.902496 1 GTATCAA 6585 0.0 37.456703 1 ATCAACG 8115 0.0 30.14571 3 TCAACGC 8260 0.0 29.616518 4 CAACGCA 8445 0.0 28.911478 5 TATCAAC 8695 0.0 28.20318 2 AACGCAG 8720 0.0 28.039793 6 TAAGGTG 1085 0.0 27.143682 5 TGGTATC 2005 0.0 24.414106 2 ACGCAGA 10080 0.0 24.303743 7 CGCAGAG 10150 0.0 24.08936 8 GTACATG 7910 0.0 23.912476 1 TACATGG 7795 0.0 23.533644 2 GTAAGGT 1005 0.0 23.159906 4 GTGGTAT 2205 0.0 22.415205 1 ACATGGG 7940 0.0 22.195261 3 GAGTACT 6075 0.0 21.72614 12-13 GCAGAGT 11275 0.0 21.391006 9 GTACTTT 6270 0.0 20.747562 14-15 CATGGGG 4100 0.0 20.159151 4 >>END_MODULE