FastQCFastQC Report
Thu 26 May 2016
SRR938446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866973
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58580.313769936683605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44160.23653261188030036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28630.15334983419685236No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36100.043.777111
GTATCAA60550.039.385811
ATCAACG77750.030.3202863
TCAACGC79300.029.7875794
CAACGCA79450.029.6707255
TATCAAC80600.029.3071352
AACGCAG82200.028.6704046
GTACATG74600.028.0118581
TACATGG75350.027.0598932
ACATGGG77800.025.4135763
ACGCAGA94600.024.8085617
CGCAGAG95900.024.3236458
CATGGGG42150.022.8902474
TACGCCG1050.002189488622.6319055
GCAGAGT104200.021.8834619
GGACCGA6600.021.5973916
GTGGTAT21300.021.0070191
GAGTACA54800.020.847161
TGGTATC20450.020.219852
GAGTACT59450.020.09580212-13
Produced by FastQC (version 0.11.5)