##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938446_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1866973 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.743582258554355 31.0 31.0 34.0 27.0 34.0 2 30.835038857016144 31.0 31.0 34.0 27.0 34.0 3 30.890049829322653 31.0 31.0 34.0 27.0 34.0 4 34.51890305858735 37.0 35.0 37.0 30.0 37.0 5 34.53234513836033 37.0 35.0 37.0 30.0 37.0 6 34.55090084323662 37.0 35.0 37.0 30.0 37.0 7 34.45823801415446 37.0 35.0 37.0 30.0 37.0 8 34.407498126646715 37.0 35.0 37.0 30.0 37.0 9 35.86308425456608 39.0 35.0 39.0 30.0 39.0 10-11 35.695703687198474 38.0 35.0 39.0 30.0 39.0 12-13 35.52555607392287 38.0 35.0 39.0 30.0 39.0 14-15 36.438654710057406 39.0 36.0 41.0 29.5 41.0 16-17 36.27899707173055 39.0 36.0 40.0 29.0 41.0 18-19 36.268045386837414 38.5 35.5 40.5 28.0 41.0 20-21 36.35614601818023 39.0 36.0 41.0 29.0 41.0 22-23 36.26002572077903 39.0 35.0 41.0 28.0 41.0 24-25 36.13101474954378 39.0 35.0 41.0 27.0 41.0 26-27 35.92197958942095 39.0 35.0 40.5 27.0 41.0 28-29 35.72389423949891 38.5 34.5 40.0 27.0 41.0 30-31 35.484781515319185 38.0 34.0 40.0 26.5 41.0 32-33 35.18526084737165 38.0 34.0 40.0 25.5 41.0 34-35 34.904997822678745 38.0 34.0 40.0 25.0 41.0 36-37 34.5921405933562 38.0 33.0 40.0 24.0 41.0 38-39 34.31134381697004 38.0 33.0 40.0 24.0 41.0 40-41 34.22758390185611 38.0 33.0 40.0 23.5 41.0 42-43 34.02549019187744 38.0 33.0 40.0 22.5 41.0 44-45 33.7045358984838 38.0 33.0 40.0 20.5 41.0 46-47 33.30901732376419 38.0 32.0 40.0 18.5 41.0 48-49 32.918562828707216 37.0 31.0 40.0 15.0 41.0 50-51 32.17672510529076 36.5 30.5 39.5 14.0 40.5 52-53 32.248074824863565 37.0 31.0 39.0 13.0 40.0 54-55 32.35189850094243 37.0 31.0 40.0 10.0 41.0 56-57 32.03526617685419 36.5 31.0 39.5 8.0 41.0 58-59 31.684713972831958 36.0 30.0 39.0 7.0 41.0 60-61 31.216905386419622 35.5 29.5 39.0 3.5 40.5 62-63 30.673112305319894 35.0 28.5 39.0 2.0 40.0 64-65 30.154722108996758 35.0 28.0 38.0 2.0 40.0 66-67 29.601056094544482 34.0 28.0 38.0 2.0 40.0 68-69 29.019721763517737 34.0 26.5 37.5 2.0 39.5 70-71 28.370331547376423 34.0 26.0 37.0 2.0 39.0 72-73 27.80324166444828 33.5 25.5 36.0 2.0 39.0 74-75 27.161093920479836 33.0 24.5 36.0 2.0 38.5 76-77 26.397830873826244 32.0 21.5 35.0 2.0 37.5 78-79 25.595200091270733 31.5 19.5 35.0 2.0 37.0 80-81 24.90851340646062 31.0 17.5 35.0 2.0 36.0 82-83 24.23093076332652 31.0 10.5 34.0 2.0 36.0 84-85 23.563329785701242 30.0 7.0 34.0 2.0 35.0 86-87 22.866908626959255 30.0 2.0 34.0 2.0 35.0 88-89 22.050665971066536 29.0 2.0 34.0 2.0 35.0 90-91 21.196966426402525 29.0 2.0 34.0 2.0 35.0 92-93 20.324416582350146 27.5 2.0 34.0 2.0 35.0 94-95 19.351297528137792 25.5 2.0 33.0 2.0 35.0 96-97 18.120280528963193 24.5 2.0 32.5 2.0 34.5 98-99 16.66310814350288 11.0 2.0 32.0 2.0 34.0 100-101 13.46927647052207 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7999652376137725 1101 2 0.8435050419965755 1101 3 0.8711159045756993 1101 4 0.7918243321056764 1101 5 0.704127455191454 1101 6 0.7688171881887698 1101 7 0.6712222598306923 1101 8 0.8576675060607997 1101 9 0.9135730316075268 1101 10-11 1.1014642748813444 1101 12-13 0.8915935098889705 1101 14-15 1.0838421393829876 1101 16-17 1.2633248791737444 1101 18-19 1.0439376562584783 1101 20-21 1.1881692279518603 1101 22-23 1.2815110598970492 1101 24-25 1.3300139665138317 1101 26-27 1.2035390057289987 1101 28-29 1.1542599583413704 1101 30-31 1.0480559156147748 1101 32-33 1.2960044761327154 1101 34-35 1.3427217574501427 1101 36-37 1.298997095851476 1101 38-39 1.3602498308201234 1101 40-41 1.2702213885247673 1101 42-43 1.4067379873450818 1101 44-45 1.2546496108060197 1101 46-47 1.282213155858095 1101 48-49 1.336479747318144 1101 50-51 1.1205473220885338 1101 52-53 1.2332292250331918 1101 54-55 1.2621649948143983 1101 56-57 1.1783845864331326 1101 58-59 1.2704850993286634 1101 60-61 1.23467737052313 1101 62-63 1.349173171324619 1101 64-65 1.203099435545834 1101 66-67 1.0334377935917338 1101 68-69 1.1549643185648932 1101 70-71 1.3748150714374923 1101 72-73 1.5001146989267795 1101 74-75 1.4711184450540813 1101 76-77 1.618434406088511 1101 78-79 1.278024321385562 1101 80-81 1.2701582376595617 1101 82-83 1.3366482251953684 1101 84-85 1.5643587665043164 1101 86-87 1.631112944360467 1101 88-89 1.6529058698879489 1101 90-91 1.377860137731826 1101 92-93 1.484497680847145 1101 94-95 1.2820022680406282 1101 96-97 1.4327006415759413 1101 98-99 1.6606307867314527 1101 100-101 1.4360244781961509 1102 1 0.6987208879477507 1102 2 0.802910056404766 1102 3 0.6190296519563177 1102 4 0.8122803915863699 1102 5 0.6826836241693002 1102 6 0.7124462531749458 1102 7 0.8174178717768541 1102 8 0.7804410639015913 1102 9 0.9382290344253548 1102 10-11 0.9071374526725648 1102 12-13 0.9313586525287931 1102 14-15 0.9184261395259128 1102 16-17 0.9250116905093861 1102 18-19 0.9315140362672025 1102 20-21 0.9607743924400722 1102 22-23 0.9649366618123665 1102 24-25 0.9275343919447323 1102 26-27 0.8760889111632792 1102 28-29 0.8432562918457123 1102 30-31 0.8468028878525189 1102 32-33 0.8000075670664017 1102 34-35 0.9106328056876052 1102 36-37 0.9442263124490964 1102 38-39 1.043835754182325 1102 40-41 0.8812636916915082 1102 42-43 1.0345470424280236 1102 44-45 1.0740247687346098 1102 46-47 1.1521171671804566 1102 48-49 1.1333882074410226 1102 50-51 1.3231197714301501 1102 52-53 1.1762415709012615 1102 54-55 1.3881647306429414 1102 56-57 1.3059261200569239 1102 58-59 1.397678232912643 1102 60-61 1.4267584162847697 1102 62-63 1.3447881803472903 1102 64-65 1.324517168469768 1102 66-67 1.1437807442975902 1102 68-69 1.1488130797666543 1102 70-71 1.2547995529595255 1102 72-73 1.535232114844959 1102 74-75 1.4969209456219872 1102 76-77 1.338639981254861 1102 78-79 1.2845893649929963 1102 80-81 1.4652152004279735 1102 82-83 1.6709217919694908 1102 84-85 1.5174456801904412 1102 86-87 1.1990970545110322 1102 88-89 1.614566870268252 1102 90-91 1.952619224847151 1102 92-93 1.7097695732591696 1102 94-95 1.784445694510353 1102 96-97 1.7662632106497576 1102 98-99 1.779871338454182 1102 100-101 1.3858834272276503 1103 1 0.779667831160797 1103 2 0.8616982996785083 1103 3 0.6615920412304597 1103 4 0.7778845819662834 1103 5 0.7965819061159962 1103 6 0.8281496625320912 1103 7 0.7290062916585285 1103 8 0.6507808927419347 1103 9 0.8874265813460624 1103 10-11 0.7846928717136308 1103 12-13 0.8375280005983186 1103 14-15 1.1008071920526348 1103 16-17 1.0660708215762114 1103 18-19 1.0014525002086287 1103 20-21 0.941835005488521 1103 22-23 0.9138482662204268 1103 24-25 1.1198948381626366 1103 26-27 1.022744213921058 1103 28-29 0.8216038331398039 1103 30-31 1.0536939794211477 1103 32-33 1.1376281932989514 1103 34-35 1.2643021860409505 1103 36-37 1.1370548742320494 1103 38-39 1.1100153039427738 1103 40-41 1.213382999206381 1103 42-43 1.2709996703627056 1103 44-45 1.2713876715984043 1103 46-47 1.1211666573726262 1103 48-49 0.9891002319693527 1103 50-51 1.0645537946535981 1103 52-53 1.1729868994307608 1103 54-55 1.207550372393257 1103 56-57 1.38984117729769 1103 58-59 1.0843762487175859 1103 60-61 1.4199385336257393 1103 62-63 1.562590745958797 1103 64-65 1.2948512477133534 1103 66-67 1.444063273947986 1103 68-69 1.5165689721809414 1103 70-71 1.5470237306595784 1103 72-73 1.6245825395464593 1103 74-75 1.4013076112309761 1103 76-77 1.2783525383126921 1103 78-79 1.369648038551798 1103 80-81 1.3254039163370166 1103 82-83 1.5254734433336203 1103 84-85 1.5183293347940214 1103 86-87 1.491914178593479 1103 88-89 1.6138915232478048 1103 90-91 1.445838082786608 1103 92-93 1.411344132615607 1103 94-95 1.2171701855173076 1103 96-97 1.216121192535148 1103 98-99 1.5600630244757738 1103 100-101 1.4091631448025375 1104 1 0.5749025713282343 1104 2 0.6263480391307823 1104 3 0.6397169805301672 1104 4 0.6569598891759725 1104 5 0.6749967941537562 1104 6 0.6299921260721888 1104 7 0.6042930177629202 1104 8 0.6104114464875181 1104 9 0.5372454958217787 1104 10-11 0.5542410143288521 1104 12-13 0.5894787976041798 1104 14-15 0.7513443565234965 1104 16-17 0.9455028257718112 1104 18-19 0.9557820423121086 1104 20-21 0.9307372263687199 1104 22-23 1.007421186915387 1104 24-25 0.9411485927515741 1104 26-27 0.9696581669535931 1104 28-29 0.9263795996410167 1104 30-31 0.73672632759979 1104 32-33 0.9483174484202905 1104 34-35 0.8601203553378198 1104 36-37 0.8569029425059682 1104 38-39 0.9685447764726973 1104 40-41 0.9636778922590921 1104 42-43 0.8820622212405809 1104 44-45 0.9814058242964947 1104 46-47 1.1603560899595422 1104 48-49 1.122682811736155 1104 50-51 0.646179573603888 1104 52-53 0.7593053627377699 1104 54-55 1.0088626464778088 1104 56-57 1.0822513894264532 1104 58-59 1.0430788605517982 1104 60-61 1.1922722678017266 1104 62-63 1.253040890491711 1104 64-65 1.1485220481802365 1104 66-67 1.0649001168618106 1104 68-69 1.1182770563119853 1104 70-71 1.0620186892244554 1104 72-73 1.0389059542314243 1104 74-75 1.0024373871299126 1104 76-77 1.2064984026583545 1104 78-79 1.3274748003612054 1104 80-81 1.3256577539198098 1104 82-83 1.3096183506012338 1104 84-85 1.4117837685198964 1104 86-87 1.4356761310327038 1104 88-89 1.3647426516993342 1104 90-91 1.464422525500538 1104 92-93 1.4698755475548566 1104 94-95 1.3919243383001607 1104 96-97 1.3875034993051507 1104 98-99 1.0881696874695201 1104 100-101 0.7076081578431683 1105 1 0.5659631099033327 1105 2 0.6722207655376593 1105 3 0.5242614495325455 1105 4 0.5909349969554256 1105 5 0.6210319951039551 1105 6 0.6999206092602748 1105 7 0.4743486793004337 1105 8 0.4606110380601294 1105 9 0.5793479724380504 1105 10-11 0.5817698045378066 1105 12-13 0.6179143638419689 1105 14-15 0.6265290312522609 1105 16-17 0.6942463092820432 1105 18-19 0.6299871075960652 1105 20-21 0.8059224613545268 1105 22-23 0.59288713869406 1105 24-25 0.7516825457292029 1105 26-27 0.7344828485175015 1105 28-29 0.8868279733548761 1105 30-31 0.7338761599428665 1105 32-33 0.6417950759558053 1105 34-35 0.6793903964326731 1105 36-37 0.7924848674931582 1105 38-39 0.9022586644044779 1105 40-41 0.8871301121375694 1105 42-43 0.8231348053421712 1105 44-45 0.955862585058334 1105 46-47 0.9105675869943823 1105 48-49 1.0665949186107824 1105 50-51 0.8022797923842617 1105 52-53 0.945975166843894 1105 54-55 1.1166412553035343 1105 56-57 1.096293812089968 1105 58-59 1.1857980401113757 1105 60-61 1.2266189660668942 1105 62-63 1.1163944771172574 1105 64-65 1.0642382387684783 1105 66-67 1.0764229235255094 1105 68-69 0.7218353888749647 1105 70-71 0.7890456291845069 1105 72-73 0.7550936011238178 1105 74-75 0.7538747265812518 1105 76-77 0.9581577439641471 1105 78-79 0.8804466838613543 1105 80-81 0.9591237281087714 1105 82-83 1.1979675252025395 1105 84-85 1.0121718865884688 1105 86-87 1.1676351538323644 1105 88-89 0.9761833807058018 1105 90-91 1.040808391335787 1105 92-93 1.054623669993429 1105 94-95 1.0287924734005465 1105 96-97 1.1992630145707537 1105 98-99 1.1436826168015024 1105 100-101 0.7972036242431528 1106 1 0.3777592192469754 1106 2 0.4166827454915101 1106 3 0.23551785486445453 1106 4 0.4235307255280034 1106 5 0.4414174423011943 1106 6 -0.00806027425105782 1106 7 0.2700867154903861 1106 8 0.3274090818766382 1106 9 0.3480621185839965 1106 10-11 0.1267823438187392 1106 12-13 0.2276933329210209 1106 14-15 0.5568672717448138 1106 16-17 0.2045399316451082 1106 18-19 0.044983169064977346 1106 20-21 0.3436370277960137 1106 22-23 0.30505580802724097 1106 24-25 0.5271684602616844 1106 26-27 0.349195280080302 1106 28-29 0.25239296468453887 1106 30-31 0.2214344376775017 1106 32-33 0.4382424652524648 1106 34-35 0.22156106982406953 1106 36-37 0.027220206554758875 1106 38-39 0.1822054176909944 1106 40-41 0.39291279429115633 1106 42-43 -0.14417079405216526 1106 44-45 0.023256279511834066 1106 46-47 -0.008484465634410299 1106 48-49 0.29832194361420505 1106 50-51 -0.24106492511311473 1106 52-53 -0.049112942132332194 1106 54-55 -0.0989447508032768 1106 56-57 -0.3516790273543471 1106 58-59 -0.7610714539474266 1106 60-61 -0.2079400437056762 1106 62-63 -0.09417887837205186 1106 64-65 0.23297005959454253 1106 66-67 -0.3251544372968205 1106 68-69 -0.44507719243551946 1106 70-71 -0.30599446589691937 1106 72-73 0.23432640867021348 1106 74-75 0.2649202882881525 1106 76-77 0.20752966655183158 1106 78-79 0.463723235545185 1106 80-81 0.5378054196452418 1106 82-83 0.28739305634755397 1106 84-85 0.43308659999422616 1106 86-87 0.5133268461760281 1106 88-89 0.5228153103633595 1106 90-91 0.6486688921833341 1106 92-93 0.4717093796666312 1106 94-95 0.27355213950217916 1106 96-97 0.31846300566382624 1106 98-99 0.20384319196620737 1106 100-101 -0.07940150113573985 1107 1 0.4760357911275257 1107 2 0.41426041564620064 1107 3 0.3816056584458387 1107 4 0.3916336093080375 1107 5 0.34293745630516526 1107 6 0.3520669932895615 1107 7 0.318064892640578 1107 8 0.3692013628377282 1107 9 0.4066332276204463 1107 10-11 0.4126452248757744 1107 12-13 0.4083165581126025 1107 14-15 0.4421121304733049 1107 16-17 0.3846809600290797 1107 18-19 0.4187497177352526 1107 20-21 0.54709004318741 1107 22-23 0.5198797535155038 1107 24-25 0.5581469713027758 1107 26-27 0.36962097476786937 1107 28-29 0.5426940824086373 1107 30-31 0.5671107936635522 1107 32-33 0.5987386336112763 1107 34-35 0.7235234892688354 1107 36-37 0.5899572528845525 1107 38-39 0.7263645150834037 1107 40-41 0.8135760215574024 1107 42-43 0.7586041472738074 1107 44-45 0.7970541101934785 1107 46-47 0.7322006266232108 1107 48-49 0.6279480344325208 1107 50-51 0.45475074117752 1107 52-53 0.4772360743482551 1107 54-55 0.39968726900974616 1107 56-57 0.4732114045643314 1107 58-59 0.5278321034493949 1107 60-61 0.6802830894227867 1107 62-63 0.5153665113402148 1107 64-65 0.7376279186731338 1107 66-67 0.7409219232275497 1107 68-69 0.6735249879044645 1107 70-71 0.7174200385830538 1107 72-73 0.7355947078364551 1107 74-75 0.8968862087248723 1107 76-77 1.073687635846838 1107 78-79 0.9676995357746918 1107 80-81 0.6547535433728946 1107 82-83 0.7709254275564312 1107 84-85 0.7870798378931099 1107 86-87 0.715403372025019 1107 88-89 1.004420515171386 1107 90-91 0.9408230233538362 1107 92-93 0.7934059668478106 1107 94-95 0.940486648261146 1107 96-97 0.9282109990767786 1107 98-99 0.6615565579754659 1107 100-101 0.49094777143593227 1108 1 0.3027491342659836 1108 2 0.2669246514594157 1108 3 0.3052896270694454 1108 4 0.30822826960137917 1108 5 0.3640297061723885 1108 6 0.3425815579829461 1108 7 0.39249701258992076 1108 8 0.206742217517629 1108 9 0.3046104102817182 1108 10-11 0.39029541689276925 1108 12-13 0.371985767983098 1108 14-15 0.6057129087549598 1108 16-17 0.4358338280276044 1108 18-19 0.357866031008939 1108 20-21 0.5248916528617968 1108 22-23 0.40478037450663606 1108 24-25 0.47982982663636164 1108 26-27 0.4298142174038162 1108 28-29 0.3043514546356221 1108 30-31 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-1.620357425846084 2315 94-95 -1.476760901744342 2315 96-97 -1.5188017750239773 2315 98-99 -1.4186266735794888 2315 100-101 -1.1584672314284887 2316 1 -0.7716923426399553 2316 2 -0.8290729688841161 2316 3 -0.8544321556476682 2316 4 -0.9909540399311751 2316 5 -0.8287009496254925 2316 6 -1.0279625985680738 2316 7 -0.7313273991412785 2316 8 -0.809319197871659 2316 9 -0.9624629919941441 2316 10-11 -1.1062028173514946 2316 12-13 -1.1038100878704782 2316 14-15 -1.3969395221653897 2316 16-17 -1.415754086355392 2316 18-19 -1.204783488629559 2316 20-21 -1.5343725522949683 2316 22-23 -1.2852453207201293 2316 24-25 -1.1229491817557289 2316 26-27 -1.4121436829290843 2316 28-29 -1.638657671600086 2316 30-31 -1.3815200709511402 2316 32-33 -1.2035851975615444 2316 34-35 -1.2566337110752102 2316 36-37 -1.490765162179656 2316 38-39 -1.572517470745268 2316 40-41 -1.6460895853316728 2316 42-43 -1.66369508374747 2316 44-45 -1.3689887212046088 2316 46-47 -1.4270634679069047 2316 48-49 -1.6874029976987046 2316 50-51 -1.7584887992783393 2316 52-53 -1.6332907938297438 2316 54-55 -1.6515547698590076 2316 56-57 -1.4698007188818458 2316 58-59 -1.2839732814495441 2316 60-61 -1.2953316583808672 2316 62-63 -1.2582926396089782 2316 64-65 -1.4060447738787296 2316 66-67 -1.3588758486796415 2316 68-69 -1.2727239369588403 2316 70-71 -1.704018187134217 2316 72-73 -1.579315646241895 2316 74-75 -1.472091617860702 2316 76-77 -1.251729235607776 2316 78-79 -1.4732446134338026 2316 80-81 -1.500296399286487 2316 82-83 -1.513449252462408 2316 84-85 -1.303741310557669 2316 86-87 -1.40466495035216 2316 88-89 -1.5634344335292347 2316 90-91 -1.646500219053653 2316 92-93 -1.5748711009984326 2316 94-95 -1.4426827267260585 2316 96-97 -1.201764323976203 2316 98-99 -1.256771706856302 2316 100-101 -1.2553792288302912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8396.0 3 3274.0 4 3365.0 5 3952.0 6 4340.0 7 5394.0 8 6762.0 9 8295.0 10 10262.0 11 12477.0 12 14610.0 13 16982.0 14 18627.0 15 21379.0 16 24109.0 17 26958.0 18 26105.0 19 24627.0 20 26204.0 21 28459.0 22 31109.0 23 34232.0 24 38235.0 25 42471.0 26 46467.0 27 52448.0 28 59387.0 29 67011.0 30 77326.0 31 88785.0 32 103185.0 33 121719.0 34 146187.0 35 174369.0 36 201182.0 37 192898.0 38 90465.0 39 4920.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.20094151762166 18.374403770792565 13.90941233223885 23.515242379346926 2 15.586426203574794 19.565010026440653 40.19032552975082 24.658238240233732 3 19.574675594352943 23.9092655557967 29.051527111282592 27.464531738567764 4 12.999712750244912 15.788822125948299 36.43907839989025 34.77238672391654 5 13.948929031773977 37.223441515460905 33.74424855359665 15.08338089916847 6 32.95469893983085 35.31821154072915 17.234555725878202 14.492533793561805 7 29.4767422546516 32.20228189199645 20.07468068621788 18.24629516713407 8 26.799349534252592 34.896094258594964 19.388219283041007 18.916336924111437 9 26.9667906407016 16.40218813010683 19.78785275935552 36.84316846983605 10-11 25.388165558719418 26.32045413870006 26.90013042448508 21.39124987809544 12-13 26.254257122710158 23.556596959519556 27.407859682663517 22.78128623510677 14-15 22.869028438672565 25.086170083375887 25.712654585084284 26.332146892867264 16-17 22.538788618504736 28.31609335200745 26.465630588974054 22.67948744051376 18-19 22.595367736082753 26.648471402204382 28.84717451405543 21.90898634765744 20-21 23.05509750972525 25.917956891114187 29.07205124921022 21.95489434995035 22-23 22.730861907138916 25.89491334414204 28.801956606023776 22.57226814269527 24-25 22.533151806867274 26.060348616575368 29.011851033950453 22.394648542606905 26-27 22.127630954540546 26.479174632760294 28.793988579200814 22.59920583349835 28-29 22.179323294269707 26.81291658438914 28.79773179286588 22.210028328475275 30-31 22.32416606866954 26.336463283333806 28.74976352540876 22.589607122587893 32-33 21.962178589553346 26.39042289177094 28.700725013973493 22.946673504702222 34-35 22.666133633575296 26.499550789220333 28.58773114661152 22.246584430592847 36-37 22.75148691583727 26.09903698118708 28.435286003887228 22.714190099088423 38-39 22.2481291306994 26.487908998174138 28.52963174091568 22.73433013021079 40-41 22.696506292873078 26.30360407081277 28.51934257530982 22.48054706100433 42-43 22.32116907590127 26.30881507980408 28.49294373065503 22.87707211363962 44-45 22.355253283653802 26.55355217395263 28.47419705083135 22.61699749156221 46-47 22.677159027296028 26.85060439104221 27.81629083623721 22.655945745424546 48-49 22.49812978952666 26.79670778674485 27.8020279429399 22.90313448078859 50-51 22.682306823078942 27.04679110475925 27.721388019242365 22.549514052919445 52-53 22.913286325951535 26.84903409526146 27.591427893415414 22.646251685371592 54-55 22.400946011458203 26.942872371483595 27.855109185042732 22.80107243201547 56-57 22.44863249889183 27.090168160519173 27.64667676406505 22.814522576523952 58-59 22.555081984512952 26.586771752833947 27.66177474594698 23.196371516706122 60-61 22.307484834191897 26.9381285383426 27.910979404927676 22.843407222537827 62-63 22.372355241309123 26.915046333494026 27.962370775081684 22.750227650115164 64-65 22.09197622521764 26.819694330348632 27.916514003252534 23.1718154411812 66-67 22.10345183143782 26.880094487809387 27.737849893473566 23.27860378727923 68-69 22.514702191729384 26.531600505353104 27.903162260182928 23.050535042734584 70-71 22.253967828777306 26.74117032532848 27.662054833049837 23.34280701284438 72-73 22.405895768142216 26.70560320835389 27.571068028052736 23.317432995451153 74-75 22.226350281711042 27.019268424523567 27.614512578557388 23.139868715208003 76-77 22.368569424330072 26.778654194403067 27.58100886806012 23.271767513206736 78-79 22.509245352148284 26.771133075220526 27.556077399559907 23.16354417307128 80-81 22.422232072432074 26.95188827183722 27.413092357323354 23.212787298407346 82-83 22.471706683693366 26.87163340603962 27.3802113910327 23.276448519234314 84-85 22.387030370652283 26.73529080133238 27.48271250845995 23.394966319555387 86-87 22.45280305158768 26.735514158615814 27.356030793352264 23.455651996444242 88-89 22.4343677335705 26.741639252917288 27.271197882622715 23.552795130889493 90-91 22.36007921530553 26.741193566328352 27.252524505627697 23.646202712738425 92-93 22.483828765457364 26.362058860400754 27.48753606852296 23.66657630561892 94-95 22.307807913597177 26.19224874566048 27.625151995490832 23.874791345251513 96-97 22.44482707164544 26.09323131171683 27.498551306262296 23.96339031037543 98-99 22.29598656384184 26.10075511423145 27.24124814837633 24.362010173550377 100-101 22.818065980363343 25.604367729299888 27.481718003556647 24.095848286780118 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 334.0 1 241.5 2 209.0 3 293.0 4 265.5 5 255.5 6 429.5 7 848.5 8 1370.5 9 1596.5 10 1487.0 11 1361.0 12 1406.5 13 1542.0 14 1681.5 15 1781.0 16 1911.5 17 2121.5 18 2301.0 19 2572.5 20 2934.5 21 3365.5 22 3973.5 23 4720.0 24 5777.5 25 7195.5 26 9112.5 27 11642.5 28 14070.0 29 16921.0 30 20137.0 31 23466.0 32 28454.5 33 31928.0 34 33870.0 35 36973.0 36 41030.0 37 45063.0 38 47647.5 39 50028.0 40 53067.5 41 56770.5 42 60031.5 43 63166.5 44 66188.5 45 68621.5 46 69734.0 47 70501.5 48 71653.0 49 70707.0 50 68808.0 51 66411.5 52 64074.0 53 63498.0 54 63041.0 55 59798.5 56 55091.5 57 50904.0 58 47598.0 59 42965.5 60 35892.5 61 29789.0 62 25078.0 63 19463.0 64 14494.5 65 11458.5 66 8676.5 67 6635.0 68 5126.0 69 3815.5 70 2940.0 71 2249.5 72 1716.5 73 1412.0 74 1088.0 75 805.0 76 601.0 77 388.0 78 213.0 79 114.0 80 71.0 81 51.5 82 36.0 83 24.0 84 14.0 85 10.5 86 7.0 87 2.5 88 3.0 89 3.0 90 2.5 91 2.0 92 2.5 93 2.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1813630941636542 2 0.048581313173784516 3 0.04649237026995034 4 0.05361620119841048 5 0.06095428268111001 6 0.06604273334429582 7 0.033583774377026344 8 0.033101710629987686 9 0.03867222504021215 10-11 0.040654042666926626 12-13 0.03690465796773708 14-15 0.03245895896726948 16-17 0.030798517171914112 18-19 0.03944888329932998 20-21 0.0510987571860975 22-23 0.04737615380618788 24-25 0.07032774442908386 26-27 0.053107356132091894 28-29 0.04443020868539609 30-31 0.05474101660816734 32-33 0.05034888024625959 34-35 0.049411534071462204 36-37 0.04617099443859124 38-39 0.024692376376091136 40-41 0.02233562027945771 42-43 0.01984495758642466 44-45 0.003963635253428946 46-47 0.011676655206047435 48-49 0.010953559585489454 50-51 0.008275427657496922 52-53 0.009319899109414009 54-55 0.010069776049251918 56-57 0.008328990296056773 58-59 0.010042994729971992 60-61 0.010257245284211396 62-63 0.003910072614869095 64-65 0.006481079265741926 66-67 0.003508352825670216 68-69 0.008543240850296174 70-71 0.021317930146820548 72-73 0.018532672941708316 74-75 0.018425547664588614 76-77 0.011649873886767512 78-79 0.004767074831826705 80-81 0.006454297946462 82-83 0.005409826494544912 84-85 0.00474029351254678 86-87 0.007579113356218864 88-89 0.027477633581203368 90-91 0.035726279919420366 92-93 0.02072874112266219 94-95 0.02959335780431747 96-97 0.03409261944334492 98-99 0.019711050990025028 100-101 0.12303338077197688 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1866973.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.966549488914964 #Duplication Level Percentage of deduplicated Percentage of total 1 81.26017844589263 49.54152690699574 2 10.971835779858282 13.378299381139557 3 3.2954983314607897 6.027454863469226 4 1.4243238691357534 3.4734444662363106 5 0.778963762344377 2.374536638351993 6 0.4761144899531439 1.741623456847071 7 0.3162422326763444 1.3496138410263119 8 0.23482543127492464 1.1453197021662795 9 0.17347790213380218 0.9518714195106238 >10 0.9607787768393038 10.693201029211579 >50 0.06886623348889079 2.8977693533915496 >100 0.034908737233666304 3.821966214973084 >500 0.0027459164211773184 1.123152712610052 >1k 0.001151513337913069 1.1638709136538052 >5k 8.857794907023607E-5 0.3163491004168518 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5858 0.313769936683605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4416 0.23653261188030036 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2863 0.15334983419685236 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.142505542394025E-4 14-15 0.0 0.0 0.0 0.0 2.142505542394025E-4 16-17 0.0 0.0 0.0 0.0 2.142505542394025E-4 18-19 0.0 0.0 0.0 2.6781319279925313E-5 2.142505542394025E-4 20-21 0.0 0.0 0.0 1.0712527711970125E-4 2.142505542394025E-4 22-23 0.0 0.0 0.0 2.678131927992531E-4 2.142505542394025E-4 24-25 0.0 0.0 0.0 4.820637470386556E-4 2.142505542394025E-4 26-27 0.0 0.0 0.0 0.0010712527711970125 2.678131927992531E-4 28-29 0.0 0.0 0.0 0.0034547901871103655 2.678131927992531E-4 30-31 0.0 0.0 0.0 0.013390659639962657 2.678131927992531E-4 32-33 0.0 0.0 0.0 0.03422652603974455 3.2137583135910373E-4 34-35 0.0 0.0 0.0 0.06542676300085754 3.2137583135910373E-4 36-37 0.0 0.0 0.0 0.1199803103740654 3.2137583135910373E-4 38-39 0.0 0.0 0.0 0.20273458694903462 3.2137583135910373E-4 40-41 0.0 0.0 0.0 0.28950606141599267 3.2137583135910373E-4 42-43 0.0 0.0 0.0 0.37566156553951235 3.2137583135910373E-4 44-45 0.0 0.0 0.0 0.4875806988103203 3.2137583135910373E-4 46-47 0.0 0.0 0.0 0.6240047392222597 3.2137583135910373E-4 48-49 0.0 0.0 0.0 0.7714894644968084 3.2137583135910373E-4 50-51 0.0 0.0 0.0 0.9281066196458116 3.2137583135910373E-4 52-53 0.0 0.0 0.0 1.0766090350529975 4.820637470386556E-4 54-55 0.0 0.0 0.0 1.2413141486245383 4.820637470386556E-4 56-57 0.0 0.0 0.0 1.4368177793679928 5.356263855985062E-4 58-59 0.0 0.0 0.0 1.6499702995169185 5.891890241583569E-4 60-61 0.0 0.0 0.0 1.8758439463238088 5.891890241583569E-4 62-63 0.0 0.0 0.0 2.0992269304376654 5.891890241583569E-4 64-65 0.0 0.0 0.0 2.336188043426445 6.427516627182075E-4 66-67 0.0 0.0 0.0 2.605742021978893 6.427516627182075E-4 68-69 0.0 0.0 0.0 2.905103608889898 6.427516627182075E-4 70-71 0.0 0.0 0.0 3.2280327567672376 6.427516627182075E-4 72-73 0.0 0.0 0.0 3.556719888289761 6.427516627182075E-4 74-75 0.0 5.3562638559850626E-5 0.0 3.8763281525763897 6.695329819981328E-4 76-77 0.0 5.3562638559850626E-5 0.0 4.232653605595796 7.498769398379088E-4 78-79 0.0 5.3562638559850626E-5 0.0 4.632391577167962 7.498769398379088E-4 80-81 0.0 5.3562638559850626E-5 0.0 5.0540634492303855 7.766582591178341E-4 82-83 0.0 5.3562638559850626E-5 0.0 5.487170944625337 8.034395783977594E-4 84-85 0.0 5.3562638559850626E-5 0.0 5.917332494899497 8.034395783977594E-4 86-87 0.0 5.3562638559850626E-5 0.0 6.356787162963792 8.034395783977594E-4 88-89 0.0 5.3562638559850626E-5 0.0 6.828459758121837 8.034395783977594E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3610 0.0 43.77711 1 GTATCAA 6055 0.0 39.38581 1 ATCAACG 7775 0.0 30.320286 3 TCAACGC 7930 0.0 29.787579 4 CAACGCA 7945 0.0 29.670725 5 TATCAAC 8060 0.0 29.307135 2 AACGCAG 8220 0.0 28.670404 6 GTACATG 7460 0.0 28.011858 1 TACATGG 7535 0.0 27.059893 2 ACATGGG 7780 0.0 25.413576 3 ACGCAGA 9460 0.0 24.808561 7 CGCAGAG 9590 0.0 24.323645 8 CATGGGG 4215 0.0 22.890247 4 TACGCCG 105 0.0021894886 22.631905 5 GCAGAGT 10420 0.0 21.883461 9 GGACCGA 660 0.0 21.597391 6 GTGGTAT 2130 0.0 21.007019 1 GAGTACA 5480 0.0 20.84716 1 TGGTATC 2045 0.0 20.21985 2 GAGTACT 5945 0.0 20.095802 12-13 >>END_MODULE