FastQCFastQC Report
Thu 26 May 2016
SRR938446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866973
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55510.29732620664573084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48450.2595109838224763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.17322157310255692No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39450.047.018811
GTATCAA67300.037.4554331
ATCAACG83200.030.1098383
TCAACGC84200.029.8086974
CAACGCA85400.029.389845
AACGCAG88350.028.59926
TATCAAC88600.028.450152
GTACATG77300.024.9804671
GTGTAAG7650.024.8686711
ACGCAGA100900.024.7595867
CGCAGAG101950.024.411418
TACATGG76150.024.295292
ACATGGG77800.022.9125183
GCAGAGT112400.021.9728229
GAGTACT61500.021.0831912-13
AGTACTT64200.020.41845312-13
GTACTTT63750.020.33907914-15
TAAGGTG11550.019.7551545
CGTAGAC1456.116861E-419.6700023
AGAGTAC102400.019.50360310-11
Produced by FastQC (version 0.11.5)