FastQCFastQC Report
Thu 26 May 2016
SRR938445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873603
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56960.30401317675089123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46980.2507468230996641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29800.1590518375557682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35300.051.295891
GTATCAA61250.039.9102971
ATCAACG80000.030.4706343
TCAACGC81350.029.9065654
CAACGCA83050.029.122745
TATCAAC83450.029.097032
AACGCAG84750.028.5938726
GTACATG77100.025.4016041
ACGCAGA96700.025.0044827
CGCAGAG96800.024.9295778
TACATGG78950.024.676632
ACATGGG81450.023.219143
GTGGTAT17200.022.9944651
TGGTATC16700.022.1938292
GAGTACT57800.022.18908112-13
GCAGAGT106800.022.1054889
AGTACTT61350.021.5632412-13
GAGTACA59700.020.9920641
GTACTTT62500.020.332914-15
AGAGTAC98600.020.13683110-11
Produced by FastQC (version 0.11.5)