FastQCFastQC Report
Thu 26 May 2016
SRR938445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873603
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57030.30438678844984773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48510.25891290737685624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32510.17351594761536995No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37150.046.6028861
GTATCAA62050.039.8594971
ATCAACG79250.030.7194393
TCAACGC80300.030.3769684
CAACGCA82200.029.6748225
TATCAAC84300.029.000522
AACGCAG85100.028.6306886
GTACATG75750.025.5553631
TACATGG74500.025.1541822
ACGCAGA99800.024.3659527
CGCAGAG100750.024.1361988
ACATGGG75800.023.398193
CATGGGG37800.022.6424454
GCAGAGT109900.021.8241659
GTGGTAT21450.021.7304651
CGTACAC2056.766404E-720.8752293
GAGTACT62450.020.80026212-13
TGGTATC20450.020.2346732
AGTACTT65050.020.18793112-13
GAGTACA57700.019.7836171
Produced by FastQC (version 0.11.5)