##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938445_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1873603 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.777627917974087 31.0 31.0 34.0 30.0 34.0 2 32.015454181061834 31.0 31.0 34.0 30.0 34.0 3 32.082915110618416 33.0 31.0 34.0 30.0 34.0 4 35.75891851155234 37.0 35.0 37.0 35.0 37.0 5 35.62985328268582 37.0 35.0 37.0 33.0 37.0 6 35.665074725008445 37.0 35.0 37.0 33.0 37.0 7 35.596291210037556 37.0 35.0 37.0 33.0 37.0 8 35.57930949085799 37.0 35.0 37.0 33.0 37.0 9 37.14238181727933 39.0 37.0 39.0 33.0 39.0 10-11 37.07494917546567 39.0 37.0 39.0 33.0 39.0 12-13 36.96944390033534 39.0 37.0 39.0 33.0 39.0 14-15 38.017985667187766 40.0 37.0 41.0 33.0 41.0 16-17 37.93507375895534 40.0 37.0 41.0 33.0 41.0 18-19 37.940859936710176 40.0 37.0 41.0 33.0 41.0 20-21 37.932536401788425 40.0 37.0 41.0 32.5 41.0 22-23 37.95895021517365 40.0 37.0 41.0 33.0 41.0 24-25 37.894033047555965 40.0 37.0 41.0 32.5 41.0 26-27 37.72517870648157 40.0 37.0 41.0 32.0 41.0 28-29 37.59075535212102 39.5 37.0 41.0 32.0 41.0 30-31 37.34795044627917 39.0 36.0 41.0 31.5 41.0 32-33 37.133160279952584 39.0 36.0 41.0 31.0 41.0 34-35 36.95952557719005 39.0 36.0 41.0 31.0 41.0 36-37 36.959209875304424 39.0 36.0 41.0 31.0 41.0 38-39 36.80925334769425 39.0 35.0 41.0 30.0 41.0 40-41 36.63131170263925 39.0 35.0 41.0 30.0 41.0 42-43 36.406131128099176 39.0 35.0 40.0 30.0 41.0 44-45 36.12311065898165 39.0 35.0 40.0 30.0 41.0 46-47 35.75562352323304 38.0 35.0 40.0 28.5 41.0 48-49 35.36557397698445 38.0 34.0 40.0 27.0 41.0 50-51 34.99830326915574 38.0 34.0 40.0 26.0 41.0 52-53 34.486438962789876 38.0 33.0 40.0 24.5 41.0 54-55 34.167100501013294 37.5 33.0 40.0 24.0 41.0 56-57 33.815554842728154 37.0 33.0 40.0 23.0 41.0 58-59 33.36300886580561 36.5 32.0 39.5 22.5 41.0 60-61 32.96266818530927 36.0 31.0 39.0 21.5 40.5 62-63 32.48798758328205 36.0 31.0 39.0 19.5 40.0 64-65 31.993389474717965 35.0 31.0 38.5 18.0 40.0 66-67 31.51307534200148 35.0 30.0 38.0 16.5 40.0 68-69 30.96086897811329 34.5 29.0 37.5 12.5 40.0 70-71 30.403033887114827 34.0 29.0 37.0 8.0 39.0 72-73 29.817276125198347 34.0 28.0 36.0 7.0 39.0 74-75 29.206961400040456 34.0 27.5 36.0 4.5 38.5 76-77 27.942516103998553 31.5 26.0 34.5 2.0 36.5 78-79 28.561247233271935 33.0 27.0 35.0 2.0 37.0 80-81 28.263670051766567 33.0 26.5 35.0 2.0 37.0 82-83 27.779556821802696 33.0 26.0 35.0 2.0 36.0 84-85 27.19730700687392 32.5 25.5 35.0 2.0 36.0 86-87 26.557824683243993 32.0 24.5 35.0 2.0 35.5 88-89 25.816590013999765 32.0 22.5 34.0 2.0 35.0 90-91 25.271247163886905 31.0 20.0 34.0 2.0 35.0 92-93 24.51388821431221 31.0 17.5 34.0 2.0 35.0 94-95 23.730371642231574 31.0 6.0 34.0 2.0 35.0 96-97 22.7644463635039 30.0 2.0 34.0 2.0 35.0 98-99 21.6040826151538 30.0 2.0 34.0 2.0 35.0 100-101 18.47420664890054 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33003841855708416 1101 2 0.3440221677344155 1101 3 0.2849009875809472 1101 4 0.31357278433033287 1101 5 0.2262304406060025 1101 6 0.2690540656761442 1101 7 0.33204045263868665 1101 8 0.3177737391891071 1101 9 0.3824288427987099 1101 10-11 0.37602191901088133 1101 12-13 0.38533664258038414 1101 14-15 0.46789734663188653 1101 16-17 0.5894247321149066 1101 18-19 0.6139030830490313 1101 20-21 0.6311484992454055 1101 22-23 0.5830597466003766 1101 24-25 0.49333442448928366 1101 26-27 0.5564185611227899 1101 28-29 0.5137803543928854 1101 30-31 0.6509537615138115 1101 32-33 0.4522332147894872 1101 34-35 0.3398481908633215 1101 36-37 0.5138460639702132 1101 38-39 0.7908233273169927 1101 40-41 0.8926368585643161 1101 42-43 0.7535857119944609 1101 44-45 0.897328386887871 1101 46-47 0.9551721374463611 1101 48-49 0.9034425932074939 1101 50-51 0.9898769126498337 1101 52-53 1.13070250668828 1101 54-55 1.1131955445546637 1101 56-57 1.1583316992704127 1101 58-59 1.2390338580562243 1101 60-61 1.4317511292782044 1101 62-63 1.380619054567795 1101 64-65 1.4475362427102851 1101 66-67 1.7135299326603501 1101 68-69 1.6219391259964198 1101 70-71 1.7310020002547155 1101 72-73 1.9904340020365652 1101 74-75 1.8508535049595594 1101 76-77 1.6752769125456766 1101 78-79 2.0272707471624223 1101 80-81 2.2752475369244074 1101 82-83 2.4269151663817574 1101 84-85 2.5660318192472893 1101 86-87 2.628005399649286 1101 88-89 2.6360757802795938 1101 90-91 2.8107284621482087 1101 92-93 3.070643409004301 1101 94-95 3.146787393931241 1101 96-97 3.694430765653749 1101 98-99 4.115152645198922 1101 100-101 4.597186406846134 1102 1 0.31937159945284677 1102 2 0.2629406231607234 1102 3 0.24980700029043135 1102 4 0.09625111247126483 1102 5 0.04765077768357884 1102 6 0.11874204502085206 1102 7 0.2660483222283503 1102 8 0.21089023007619545 1102 9 0.23808665855884925 1102 10-11 0.27141496976306456 1102 12-13 0.27588551311324494 1102 14-15 0.32798680843198014 1102 16-17 0.41385237666860064 1102 18-19 0.5188382494441726 1102 20-21 0.474236884455145 1102 22-23 0.4843869062120376 1102 24-25 0.36677122482464597 1102 26-27 0.3697187392842025 1102 28-29 0.4173697828472669 1102 30-31 0.36676820687922884 1102 32-33 0.3236154424740647 1102 34-35 0.44854189614432727 1102 36-37 0.5530458619904337 1102 38-39 0.6239146660046941 1102 40-41 0.5733944732974052 1102 42-43 0.636624650266775 1102 44-45 0.5564803576198614 1102 46-47 0.5835431514420364 1102 48-49 0.7448841762716754 1102 50-51 0.9262680484050207 1102 52-53 0.949221289855366 1102 54-55 0.794808238793479 1102 56-57 0.9033031648623933 1102 58-59 1.028369897156459 1102 60-61 0.9845845626486849 1102 62-63 1.1524700277864284 1102 64-65 1.1088271031251011 1102 66-67 1.0747169449792153 1102 68-69 1.2319938129753965 1102 70-71 1.3598913019953613 1102 72-73 1.3341631014163333 1102 74-75 1.6020158414464447 1102 76-77 1.7713454522890117 1102 78-79 2.011079495173586 1102 80-81 2.033332444652043 1102 82-83 1.9814520896199177 1102 84-85 1.9325269936454 1102 86-87 2.1339695196557678 1102 88-89 2.3919612996629844 1102 90-91 2.642070103329221 1102 92-93 2.981233056587694 1102 94-95 2.8741242142740333 1102 96-97 3.1093101017881217 1102 98-99 3.385948940965921 1102 100-101 3.7838881341543704 1103 1 0.231574965756419 1103 2 0.21716307131215729 1103 3 0.2544905503719974 1103 4 0.19404323800453227 1103 5 0.13894764679240978 1103 6 0.17720247916759746 1103 7 0.19347908815964132 1103 8 0.19021958297032882 1103 9 0.27848786632139166 1103 10-11 0.3605923456619635 1103 12-13 0.48378061359650815 1103 14-15 0.5375906027922142 1103 16-17 0.5949487606965818 1103 18-19 0.4748986650830531 1103 20-21 0.38892990105406966 1103 22-23 0.3641071124745707 1103 24-25 0.33906634256523915 1103 26-27 0.3977922154656639 1103 28-29 0.2584099805311624 1103 30-31 0.4942765369768125 1103 32-33 0.5414861826194084 1103 34-35 0.5004742474359105 1103 36-37 0.6405065718791931 1103 38-39 0.6646303822367727 1103 40-41 0.5679179304395277 1103 42-43 0.47944998051629995 1103 44-45 0.674499191862644 1103 46-47 0.8327470323389932 1103 48-49 0.8319673822072033 1103 50-51 0.9448277996994747 1103 52-53 0.9478794704207445 1103 54-55 0.9065456573604891 1103 56-57 1.005045247908889 1103 58-59 1.2118064930618502 1103 60-61 1.222644651899067 1103 62-63 1.2192941675307267 1103 64-65 1.1785163650056134 1103 66-67 1.3194159453675027 1103 68-69 1.233300148601586 1103 70-71 1.2330557574111296 1103 72-73 1.215179639059496 1103 74-75 1.4085965716438196 1103 76-77 1.1611171116369476 1103 78-79 1.4741241247382568 1103 80-81 1.8321456597602008 1103 82-83 2.0825259203268196 1103 84-85 2.1156151604361817 1103 86-87 2.0098546609125876 1103 88-89 2.2368154596471292 1103 90-91 2.414843517796985 1103 92-93 2.5164963026777514 1103 94-95 2.6041537080404495 1103 96-97 2.9806728551065618 1103 98-99 3.26750742734858 1103 100-101 3.8214779926005527 1104 1 0.33273405505185494 1104 2 0.26974040309924874 1104 3 0.2567365447392618 1104 4 0.237764393898793 1104 5 0.06504520199558783 1104 6 0.1798366116552259 1104 7 0.28860073632663585 1104 8 0.27279537640511364 1104 9 0.23323723323024836 1104 10-11 0.2975329179290256 1104 12-13 0.3713596020707044 1104 14-15 0.48662706174187775 1104 16-17 0.4645870112349968 1104 18-19 0.5542850400150172 1104 20-21 0.4322694272415575 1104 22-23 0.4175876345676812 1104 24-25 0.4339282458620417 1104 26-27 0.502028009373781 1104 28-29 0.5449514687095132 1104 30-31 0.5702048734262988 1104 32-33 0.5073329863966549 1104 34-35 0.6458630363976425 1104 36-37 0.5941066121130163 1104 38-39 0.634773922340429 1104 40-41 0.6669546852261803 1104 42-43 0.6125035506769976 1104 44-45 0.6715275093785564 1104 46-47 0.7099548637355824 1104 48-49 0.7571780181383687 1104 50-51 0.8813888131186403 1104 52-53 1.095297109404953 1104 54-55 0.9405470892114778 1104 56-57 1.1014739038623063 1104 58-59 1.2174717712669505 1104 60-61 1.0665900522256706 1104 62-63 1.097700434541352 1104 64-65 1.1245166490091165 1104 66-67 1.3029322045680374 1104 68-69 1.2326720866888152 1104 70-71 1.3047518499962685 1104 72-73 1.3195030007142918 1104 74-75 1.441837998288083 1104 76-77 1.4335306089034106 1104 78-79 1.6945895354783254 1104 80-81 2.0472709171383734 1104 82-83 2.117182926929903 1104 84-85 2.2760164928859474 1104 86-87 2.3778942084003383 1104 88-89 2.5185661637391483 1104 90-91 2.651493578147729 1104 92-93 2.840548145150475 1104 94-95 3.128320631099168 1104 96-97 3.499084269680676 1104 98-99 3.934486399255899 1104 100-101 4.108307034325399 1105 1 0.3250121181479706 1105 2 0.33989202941374685 1105 3 0.3194280229846598 1105 4 0.1443752567458958 1105 5 0.11646475334177353 1105 6 0.24111187344406204 1105 7 0.30518569819772523 1105 8 0.3134204458819809 1105 9 0.3262345208533617 1105 10-11 0.42746799874451114 1105 12-13 0.39765798576087263 1105 14-15 0.6500219237886071 1105 16-17 0.6077154711829706 1105 18-19 0.5940883371092198 1105 20-21 0.5387596782475796 1105 22-23 0.4035280178576528 1105 24-25 0.4079138836041025 1105 26-27 0.4131459755357767 1105 28-29 0.5510162433821364 1105 30-31 0.6841002468227728 1105 32-33 0.6993720490703481 1105 34-35 0.784653638678698 1105 36-37 0.8671809912677375 1105 38-39 1.0063990140498973 1105 40-41 0.9114899127768084 1105 42-43 0.9609108629782881 1105 44-45 0.9522404531967865 1105 46-47 1.0747920655408691 1105 48-49 1.3484756236876265 1105 50-51 1.368370793598423 1105 52-53 1.3638888411019678 1105 54-55 1.4566771165575716 1105 56-57 1.3288477894533628 1105 58-59 1.6082402409972687 1105 60-61 1.7819866817639038 1105 62-63 1.8014477388389167 1105 64-65 1.7539537196420767 1105 66-67 1.9028335474169182 1105 68-69 1.9409551187704537 1105 70-71 1.9641290037791457 1105 72-73 2.077897473690566 1105 74-75 2.1207514912514682 1105 76-77 2.0182732110066155 1105 78-79 2.342909306927222 1105 80-81 2.6507066871643907 1105 82-83 2.5211361606612 1105 84-85 2.6252892090423785 1105 86-87 3.0604833055345573 1105 88-89 3.143664820890262 1105 90-91 3.410158177182833 1105 92-93 3.7085868409863103 1105 94-95 3.335614590502445 1105 96-97 3.7119989076228244 1105 98-99 4.289889198339917 1105 100-101 4.743099659772827 1106 1 0.12240772414244105 1106 2 0.10065879792822585 1106 3 0.16989526634802132 1106 4 0.10386099143896388 1106 5 -0.034689962740920066 1106 6 0.05825702641418218 1106 7 0.08752568470620048 1106 8 0.13631768540951583 1106 9 0.174630087166868 1106 10-11 0.20785700447179778 1106 12-13 0.1453631032826621 1106 14-15 0.12263792447641464 1106 16-17 0.0821435692743222 1106 18-19 0.26412209899697103 1106 20-21 0.3937852724700335 1106 22-23 0.2581410953810348 1106 24-25 0.08896572678492731 1106 26-27 0.11340919260387494 1106 28-29 0.2528617389312515 1106 30-31 0.1734087068045156 1106 32-33 0.21259490398351488 1106 34-35 0.10758311899032691 1106 36-37 0.22788096349106723 1106 38-39 0.17134699891821725 1106 40-41 0.1333800824790714 1106 42-43 0.2585792756074241 1106 44-45 -0.14943375285324123 1106 46-47 -0.48722845613155386 1106 48-49 -0.0042873288407108134 1106 50-51 -0.11019817853231473 1106 52-53 0.15049618472590254 1106 54-55 0.12097284556303833 1106 56-57 0.32302543452035337 1106 58-59 0.6848162366588113 1106 60-61 0.4039924222768434 1106 62-63 0.5497753514997896 1106 64-65 0.36162388484716956 1106 66-67 0.44149603435526785 1106 68-69 0.26541066286262094 1106 70-71 0.42410165698073854 1106 72-73 0.7947150933617486 1106 74-75 0.9887486276084871 1106 76-77 0.9823354836677538 1106 78-79 1.1684841849082552 1106 80-81 1.1652067731398112 1106 82-83 1.5947931253319148 1106 84-85 1.5493803496055811 1106 86-87 1.8168463136162458 1106 88-89 1.9607512790995933 1106 90-91 2.083352362598756 1106 92-93 1.8663426509409398 1106 94-95 2.4796201193835756 1106 96-97 2.684401623123531 1106 98-99 3.0750432266192185 1106 100-101 3.378037261455301 1107 1 0.23063642293105957 1107 2 0.2209693838816733 1107 3 0.10263779731303657 1107 4 0.14642087982878138 1107 5 -0.0030677870229425253 1107 6 0.05743398639784658 1107 7 -0.007542880694643372 1107 8 0.1022051224586491 1107 9 0.1345605860099326 1107 10-11 0.21907225389333007 1107 12-13 0.22409102794578928 1107 14-15 0.2566360699779793 1107 16-17 0.2972568808300622 1107 18-19 0.31387773905413496 1107 20-21 0.16322773197732232 1107 22-23 0.1774066119183999 1107 24-25 0.14382336425600073 1107 26-27 0.22388370601071017 1107 28-29 0.1659374415990129 1107 30-31 0.20963735455189436 1107 32-33 0.3812734462456575 1107 34-35 0.4184125816961952 1107 36-37 0.312042653636702 1107 38-39 0.39862301293754854 1107 40-41 0.47389853388781233 1107 42-43 0.4575071968678017 1107 44-45 0.46675099942660125 1107 46-47 0.6332979917383241 1107 48-49 0.6003993204719364 1107 50-51 0.6472315121577665 1107 52-53 0.8070024542917125 1107 54-55 0.6821383297742827 1107 56-57 0.5859250449055438 1107 58-59 0.5179018072998929 1107 60-61 0.8647991290959496 1107 62-63 1.0506345457287196 1107 64-65 1.053377321624076 1107 66-67 1.215941598871396 1107 68-69 1.220560298768433 1107 70-71 1.1465447034622969 1107 72-73 1.1669142052441472 1107 74-75 1.2759491856593925 1107 76-77 1.2068276233165918 1107 78-79 1.5028192026025486 1107 80-81 1.7910670307279446 1107 82-83 1.8216023246649762 1107 84-85 1.6905508528722244 1107 86-87 2.041400128098349 1107 88-89 2.2554559629841684 1107 90-91 2.4114369549493766 1107 92-93 2.5506749039013314 1107 94-95 2.660535799252912 1107 96-97 3.041095894594882 1107 98-99 3.292587377292964 1107 100-101 3.5416010459932075 1108 1 0.11285724943235209 1108 2 0.0842244429754615 1108 3 0.09680122045052997 1108 4 0.05396812238929982 1108 5 0.03272662057807452 1108 6 0.0643958209839468 1108 7 0.03584853853031689 1108 8 0.0699612631797919 1108 9 0.10645222643073282 1108 10-11 0.10261809926729626 1108 12-13 0.17799769649295882 1108 14-15 0.19129476885941443 1108 16-17 0.11218496174308967 1108 18-19 0.29384416903449306 1108 20-21 0.2705193817329885 1108 22-23 0.17943846817763642 1108 24-25 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-0.14130641629812857 2316 62-63 -0.04218221516253351 2316 64-65 -0.16236095572876508 2316 66-67 0.08092902404598945 2316 68-69 -0.015899420220591765 2316 70-71 0.031402431264091035 2316 72-73 0.1359375258294513 2316 74-75 0.31150788203451896 2316 76-77 0.42109641280777765 2316 78-79 0.37359260632719327 2316 80-81 0.5330435646487963 2316 82-83 0.790924427258922 2316 84-85 1.0790407306573897 2316 86-87 1.2354068208153421 2316 88-89 1.4501054267354156 2316 90-91 1.4670945871409309 2316 92-93 1.3706506895681123 2316 94-95 1.8504800958940812 2316 96-97 2.038958693896511 2316 98-99 2.322622503664764 2316 100-101 2.9852564749922124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 31.0 5 63.0 6 154.0 7 339.0 8 831.0 9 1409.0 10 2409.0 11 3826.0 12 5288.0 13 7722.0 14 9980.0 15 13116.0 16 16320.0 17 20197.0 18 19715.0 19 17075.0 20 17197.0 21 18522.0 22 20140.0 23 22913.0 24 26245.0 25 30691.0 26 36345.0 27 43627.0 28 51819.0 29 61184.0 30 72687.0 31 84902.0 32 101482.0 33 123488.0 34 153420.0 35 192932.0 36 245839.0 37 279290.0 38 161671.0 39 10721.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78350322880568 19.25477275602142 14.593913438439198 23.367810576733707 2 16.079360900830377 20.332813423602904 39.33048584416271 24.257339831404007 3 19.660130049172743 23.953257874283665 29.646258350906464 26.740353725637128 4 13.401878626368552 16.516839479868466 36.064150196172825 34.01713169759015 5 13.753104796118611 37.43792411287914 34.007354807204784 14.801616283797467 6 33.109575507724955 35.785862853550086 16.80484072666408 14.299720912060879 7 29.44401775616286 32.2360179824648 20.03738251913559 18.28258174223675 8 26.7373077434227 35.467065328140485 19.13575074335385 18.659876185082965 9 26.573078715181392 16.47205944909354 19.846200075469564 37.10866176025551 10-11 25.44039479014498 26.5246693136166 26.86260109532276 21.172334800915667 12-13 26.19618457058406 23.639052670176124 27.483116754189655 22.681646005050162 14-15 22.720821860340745 25.215427174273312 25.733733346925682 26.33001761846026 16-17 22.4469377984557 28.48487646529174 26.538573006127763 22.529612730124793 18-19 22.456571642978794 26.814805484406246 28.906417207914377 21.82220566470058 20-21 22.91253803500528 25.98069067993593 29.182889865142187 21.9238814199166 22-23 22.683273884595618 25.95002783407157 28.933263877139392 22.433434404193417 24-25 22.52691767591828 26.17431413500229 29.07317156485728 22.225596624222145 26-27 22.150234867086578 26.512944379203585 28.950918116792995 22.385902636916835 28-29 22.190506740221917 26.79161487252102 28.896463124792177 22.12141526246489 30-31 22.32951697878366 26.401324079861098 28.83852662490399 22.430632316451245 32-33 21.98578134548822 26.408028415959695 28.756534896816017 22.849655341736074 34-35 22.65045172149006 26.530406892312552 28.67060243144107 22.14853895475632 36-37 22.777961623655628 26.127058258386487 28.547783619332286 22.547196498625606 38-39 22.23608296481818 26.480886454095543 28.719921276675812 22.563109304410464 40-41 22.701477201457184 26.323676590293438 28.564192209663858 22.410653998585516 42-43 22.336846796716852 26.34846671283675 28.562122115870903 22.752564374575496 44-45 22.384181285504066 26.56047093410221 28.55107381536578 22.50427396502794 46-47 22.730100290803566 26.87794069336516 27.88212968446065 22.509829331370625 48-49 22.49315890292661 26.80719981767749 27.91930841272137 22.78033286667453 50-51 22.66566076164481 26.959393211902416 27.853019022706516 22.52192700374626 52-53 22.980188439066335 26.8106690691678 27.634856477065846 22.574286014700018 54-55 22.501918096287767 26.977680698013586 27.795143313482985 22.725257892215662 56-57 22.495507319320048 27.074919286529752 27.651161959070304 22.778411435079896 58-59 22.61863906067614 26.632829900464504 27.67571358500173 23.07281745385762 60-61 22.326875010341038 27.007749240367357 27.87842461823556 22.786951131056046 62-63 22.399035441339493 26.96982765292327 27.8895795961044 22.74155730963283 64-65 22.098251337129586 26.901750264063413 27.774267013876475 23.22573138493053 66-67 22.119819726536853 27.005472892263978 27.661563473060923 23.213143908138246 68-69 22.522019804146833 26.625062213202146 27.811976691898877 23.040941290752148 70-71 22.254394340743477 26.888967406649112 27.503718770732117 23.352919481875293 72-73 22.42935734767647 26.932019998905847 27.363781805372273 23.27484084804541 74-75 22.227574358068384 27.21705185143277 27.274054322073564 23.281319468425274 76-77 22.26955230110114 26.984104957133393 27.51791601529246 23.228426726473003 78-79 22.44440257621278 27.058480371775662 27.3678842316115 23.129232820400052 80-81 22.311281240968263 27.138881987445036 27.32256565763851 23.227271113948188 82-83 22.31098280056971 27.19705782434105 27.246828864214194 23.24513051087504 84-85 22.293929748271353 27.062157009947395 27.270109428555187 23.37380381322606 86-87 22.308874952930783 27.22662424499105 27.086277244437827 23.378223557640347 88-89 22.223737425627903 27.319695613130317 26.918089616127222 23.538477345114558 90-91 22.147943845547232 27.24908030471036 27.07278702699241 23.53018882275 92-93 22.172718526791375 26.975110716149015 27.207097575653254 23.64507318140636 94-95 21.991451764327874 26.820409659890597 27.305891376134646 23.882247199646883 96-97 22.11082070214448 26.692901324346725 27.23808085277404 23.958197120734756 98-99 21.893805678150603 26.90105641376535 26.938097345061895 24.267040563022153 100-101 21.98555233150058 26.76920424671466 27.172013933681843 24.073229488102918 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 317.0 1 236.0 2 222.0 3 314.0 4 263.5 5 219.5 6 412.0 7 907.0 8 1476.5 9 1706.0 10 1504.5 11 1324.5 12 1377.5 13 1503.5 14 1649.5 15 1801.5 16 1943.5 17 2063.5 18 2252.0 19 2491.0 20 2829.5 21 3294.0 22 3895.5 23 4694.0 24 5755.5 25 7229.0 26 9258.0 27 11745.0 28 14029.0 29 16945.0 30 20188.0 31 23800.5 32 29350.0 33 32560.5 34 34110.5 35 37404.5 36 41671.0 37 45615.5 38 48085.5 39 50187.0 40 53336.5 41 57342.5 42 60494.5 43 64124.5 44 67330.5 45 69873.5 46 70987.0 47 71808.5 48 73068.0 49 71469.0 50 69832.5 51 67425.5 52 64586.0 53 64246.0 54 64361.5 55 60716.5 56 55202.0 57 51100.0 58 47510.5 59 42346.5 60 34764.0 61 28575.0 62 24214.5 63 18276.5 64 13062.5 65 10220.0 66 7574.0 67 5785.5 68 4427.5 69 3229.0 70 2546.5 71 1884.0 72 1416.5 73 1233.0 74 936.0 75 699.5 76 480.5 77 270.5 78 155.5 79 91.0 80 57.0 81 28.0 82 17.0 83 10.5 84 6.0 85 4.5 86 2.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.04494015007448216 3 2.1349239940371574E-4 4 0.0 5 0.10151563591646681 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010674619970185786 26-27 0.010834739269738573 28-29 0.0 30-31 0.0 32-33 4.269847988074315E-4 34-35 0.014384050409825346 36-37 0.0028287742920992337 38-39 0.011021545119216825 40-41 0.006831756780918904 42-43 0.005097131035763713 44-45 0.0026686549925464464 46-47 8.005964977639339E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.6686549925464468E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.6686549925464465E-4 68-69 2.9355204918010913E-4 70-71 0.0 72-73 2.6686549925464468E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.005964977639339E-5 82-83 0.0012542678464968298 84-85 0.005203877235465571 86-87 0.0017346257451551902 88-89 2.6686549925464468E-5 90-91 0.001094148546944043 92-93 2.9355204918010913E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.205368479875406E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1873603.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.69872793713009 #Duplication Level Percentage of deduplicated Percentage of total 1 78.91655047294964 45.533845754755156 2 12.26994745439043 14.159207199475105 3 3.7433257321727185 6.479553990020759 4 1.6098950671868684 3.7155558995577156 5 0.8564898951885718 2.470918872169323 6 0.5496260702705078 1.9027635057415189 7 0.36261035047991474 1.4645509171669593 8 0.2556737041210144 1.1801637995805363 9 0.2021164147799638 1.0495674025215542 >10 1.110187650005191 11.770394119850994 >50 0.08006386172845013 3.1986504825945064 >100 0.03887519876601702 4.170144795859832 >500 0.003061164454179735 1.174718780283335 >1k 0.0014842009474810838 1.4247238569510705 >5k 9.276255921756774E-5 0.30524062347165387 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5703 0.30438678844984773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4851 0.25891290737685624 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3251 0.17351594761536995 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.337309985092893E-5 0.0 10-11 0.0 0.0 0.0 5.337309985092893E-5 0.0 12-13 0.0 0.0 0.0 1.0674619970185786E-4 1.8680584947825126E-4 14-15 0.0 0.0 0.0 1.0674619970185786E-4 2.4017894932918017E-4 16-17 0.0 0.0 0.0 1.601192995527868E-4 2.6686549925464465E-4 18-19 0.0 0.0 0.0 2.1349239940371572E-4 3.736116989565025E-4 20-21 0.0 0.0 0.0 4.00298248881967E-4 4.803578986583604E-4 22-23 0.0 0.0 0.0 7.739099478384695E-4 4.803578986583604E-4 24-25 0.0 0.0 0.0 9.874023472421852E-4 5.337309985092893E-4 26-27 0.0 0.0 0.0 0.0018413719448570481 5.871040983602183E-4 28-29 0.0 0.0 0.0 0.004456653837552566 5.871040983602183E-4 30-31 0.0 0.0 0.0 0.014143871460496168 5.871040983602183E-4 32-33 0.0 0.0 0.0 0.034745888002954736 5.871040983602183E-4 34-35 0.0 0.0 0.0 0.06741022511172325 6.404771982111472E-4 36-37 0.0 0.0 0.0 0.12409245715340977 6.404771982111472E-4 38-39 0.0 0.0 0.0 0.20385855488062304 6.671637481366116E-4 40-41 0.0 0.0 0.0 0.291764050335103 6.938502980620761E-4 42-43 0.0 0.0 0.0 0.3818845294333965 6.938502980620761E-4 44-45 0.0 0.0 0.0 0.4926337116240741 7.205368479875406E-4 46-47 0.0 0.0 0.0 0.627080550148564 7.47223397913005E-4 48-49 0.0 0.0 0.0 0.7680122203049419 7.47223397913005E-4 50-51 0.0 0.0 0.0 0.9235681198204742 7.47223397913005E-4 52-53 0.0 0.0 0.0 1.0711447409082928 7.47223397913005E-4 54-55 0.0 0.0 0.0 1.236521290796396 7.47223397913005E-4 56-57 0.0 0.0 0.0 1.433654835095802 7.47223397913005E-4 58-59 0.0 0.0 0.0 1.6480545771969837 7.47223397913005E-4 60-61 0.0 0.0 0.0 1.8778257720552327 7.47223397913005E-4 62-63 0.0 0.0 0.0 2.103540611324811 7.47223397913005E-4 64-65 0.0 0.0 0.0 2.3401969360638297 7.47223397913005E-4 66-67 0.0 0.0 0.0 2.6145880423974557 7.47223397913005E-4 68-69 0.0 0.0 0.0 2.916546354804086 7.47223397913005E-4 70-71 0.0 0.0 0.0 3.2496478709737335 7.47223397913005E-4 72-73 0.0 0.0 0.0 3.578559598805083 8.806561475403273E-4 74-75 0.0 0.0 0.0 3.9161444553622085 0.0010140888971676496 76-77 0.0 0.0 0.0 4.283591561285929 0.0010140888971676496 78-79 0.0 0.0 0.0 4.6835428850188645 0.0010140888971676496 80-81 0.0 0.0 0.0 5.116478784459675 0.0010140888971676496 82-83 0.0 0.0 0.0 5.556219754131478 0.0010674619970185786 84-85 0.0 0.0 0.0 5.99532024660507 0.0010674619970185786 86-87 0.0 0.0 0.0 6.4609204831546485 0.0011208350968695076 88-89 0.0 0.0 0.0 6.9608929960082255 0.001200894746645901 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3715 0.0 46.602886 1 GTATCAA 6205 0.0 39.859497 1 ATCAACG 7925 0.0 30.719439 3 TCAACGC 8030 0.0 30.376968 4 CAACGCA 8220 0.0 29.674822 5 TATCAAC 8430 0.0 29.00052 2 AACGCAG 8510 0.0 28.630688 6 GTACATG 7575 0.0 25.555363 1 TACATGG 7450 0.0 25.154182 2 ACGCAGA 9980 0.0 24.365952 7 CGCAGAG 10075 0.0 24.136198 8 ACATGGG 7580 0.0 23.39819 3 CATGGGG 3780 0.0 22.642445 4 GCAGAGT 10990 0.0 21.824165 9 GTGGTAT 2145 0.0 21.730465 1 CGTACAC 205 6.766404E-7 20.875229 3 GAGTACT 6245 0.0 20.800262 12-13 TGGTATC 2045 0.0 20.234673 2 AGTACTT 6505 0.0 20.187931 12-13 GAGTACA 5770 0.0 19.783617 1 >>END_MODULE