FastQCFastQC Report
Thu 26 May 2016
SRR938444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873475
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55600.29677471009754597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.23432391678565234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27610.14737319686678496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38950.047.6289251
GTATCAA67000.040.928111
ATCAACG81650.032.9397353
TCAACGC83400.032.1915784
CAACGCA84300.031.8478955
TATCAAC86700.031.2403322
AACGCAG87550.030.7692726
GTACATG74800.027.1604291
TACATGG74450.026.934422
ACGCAGA100100.026.763487
CGCAGAG101700.026.2490068
ACATGGG77100.025.1458113
GCAGAGT110500.023.599139
GAGTACT62050.022.54391912-13
GTACTTT65550.021.1607414-15
GTAAGGT8500.020.684394
TAAGGTG10200.020.4980435
CATGGGG41650.020.4219824
CAGAGTA107100.020.35678310-11
TACCTGG14050.020.2924652
Produced by FastQC (version 0.11.5)