##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938444_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1873475 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.750292904895982 31.0 31.0 34.0 27.0 34.0 2 30.92784371288648 31.0 31.0 34.0 27.0 34.0 3 30.968165574667395 31.0 31.0 34.0 27.0 34.0 4 34.64205233589986 37.0 35.0 37.0 32.0 37.0 5 34.6253331376186 37.0 35.0 37.0 30.0 37.0 6 34.586470596084816 37.0 35.0 37.0 30.0 37.0 7 34.480284498058424 37.0 35.0 37.0 30.0 37.0 8 34.4238791550461 37.0 35.0 37.0 30.0 37.0 9 35.8473558494242 38.0 35.0 39.0 30.0 39.0 10-11 35.65894073846729 38.0 35.0 39.0 30.0 39.0 12-13 35.44718451006819 38.0 35.0 39.0 30.0 39.0 14-15 36.543043808964626 39.0 36.0 41.0 30.0 41.0 16-17 36.45789775684223 39.0 36.0 41.0 29.0 41.0 18-19 36.44749462896489 39.0 36.0 41.0 29.0 41.0 20-21 36.36305475119764 39.0 36.0 41.0 29.0 41.0 22-23 36.18697313815237 39.0 35.0 40.5 27.5 41.0 24-25 36.02720826272035 39.0 35.0 40.5 27.0 41.0 26-27 35.783023525800985 38.5 34.5 40.0 27.0 41.0 28-29 35.5216618849998 38.0 34.0 40.0 27.0 41.0 30-31 35.22160984267204 38.0 34.0 40.0 25.5 41.0 32-33 34.891272901960264 38.0 34.0 40.0 25.0 41.0 34-35 34.546282976821146 38.0 33.0 40.0 24.0 41.0 36-37 34.278641028036134 38.0 33.0 40.0 24.0 41.0 38-39 33.99368659843339 38.0 33.0 40.0 23.0 41.0 40-41 33.67780167869867 38.0 32.5 40.0 21.5 41.0 42-43 33.219432338301814 37.0 31.5 40.0 18.5 41.0 44-45 32.71013490972658 37.0 31.0 40.0 15.5 41.0 46-47 32.16525894394107 37.0 30.0 39.5 11.0 41.0 48-49 31.638104057967148 36.0 30.0 39.0 8.5 40.5 50-51 30.916053590253405 35.5 29.0 38.5 7.5 40.0 52-53 30.900333070897666 35.5 29.0 39.0 4.0 40.0 54-55 30.827758843859673 36.0 29.0 39.0 2.0 40.0 56-57 30.35574827526388 35.0 28.0 39.0 2.0 40.0 58-59 29.85804107340637 35.0 27.5 39.0 2.0 40.0 60-61 29.26591868052683 34.5 26.5 38.0 2.0 40.0 62-63 28.50112011102363 34.0 25.5 38.0 2.0 40.0 64-65 27.792562484153777 33.5 23.5 37.5 2.0 40.0 66-67 27.04811940378174 33.0 22.0 37.0 2.0 39.0 68-69 26.15204366217857 32.0 19.0 36.0 2.0 39.0 70-71 25.279637305008073 31.0 14.0 35.5 2.0 39.0 72-73 24.347455663940003 31.0 7.5 35.0 2.0 38.0 74-75 23.375790709777284 30.0 2.0 34.5 2.0 37.0 76-77 22.39627029984387 29.0 2.0 34.0 2.0 36.5 78-79 21.382739294626298 28.5 2.0 34.0 2.0 36.0 80-81 20.388998518795287 26.5 2.0 33.5 2.0 35.0 82-83 19.367508240035228 25.5 2.0 33.0 2.0 35.0 84-85 18.38264988857604 24.5 2.0 32.0 2.0 35.0 86-87 17.379902053670317 21.0 2.0 32.0 2.0 34.0 88-89 16.300491866718264 14.5 2.0 31.0 2.0 34.0 90-91 15.166553330041767 4.5 2.0 31.0 2.0 34.0 92-93 14.030698835052508 2.0 2.0 30.0 2.0 34.0 94-95 12.828391091421022 2.0 2.0 29.0 2.0 34.0 96-97 11.497521450779967 2.0 2.0 28.0 2.0 33.5 98-99 9.948386020630112 2.0 2.0 22.5 2.0 32.5 100-101 7.064731581686438 2.0 2.0 2.0 2.0 28.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6639323431134443 1101 2 0.629331924003715 1101 3 0.5247280851534697 1101 4 0.5507131279415631 1101 5 0.5290537995379978 1101 6 0.6203175143523652 1101 7 0.5055962511688747 1101 8 0.604783349937172 1101 9 0.6305849681332276 1101 10-11 0.8019109035743384 1101 12-13 0.7460017294968537 1101 14-15 0.9285022284584059 1101 16-17 0.7418445202796988 1101 18-19 0.8458628580682586 1101 20-21 1.0436671219106373 1101 22-23 1.1164118511654237 1101 24-25 1.0802509318797675 1101 26-27 0.9506948947441529 1101 28-29 1.2119706404278148 1101 30-31 1.1017269457018344 1101 32-33 1.0675159461476653 1101 34-35 1.1803053120604545 1101 36-37 1.3334111527284378 1101 38-39 1.2543000674437863 1101 40-41 1.478447002081161 1101 42-43 1.7295242559796975 1101 44-45 1.9008562765019619 1101 46-47 1.796964539678477 1101 48-49 1.7757254053636835 1101 50-51 1.8564168317854701 1101 52-53 1.717566925236369 1101 54-55 1.9057257171308635 1101 56-57 2.2797924299041448 1101 58-59 2.0836448912176735 1101 60-61 2.1228522223057844 1101 62-63 2.110377361563515 1101 64-65 2.211576588515605 1101 66-67 2.4092945831650816 1101 68-69 2.534219987182844 1101 70-71 2.610735206389421 1101 72-73 2.9699066192355303 1101 74-75 2.937525595776261 1101 76-77 3.154371049201 1101 78-79 3.135163285081248 1101 80-81 3.5704622046039916 1101 82-83 3.611239240590585 1101 84-85 3.6502845274394815 1101 86-87 3.6073348611125944 1101 88-89 4.0885810363219655 1101 90-91 4.137293709852342 1101 92-93 4.239012416738127 1101 94-95 4.087510119425335 1101 96-97 3.555635161933152 1101 98-99 2.9503633289031317 1101 100-101 2.234559474586529 1102 1 0.803274398719843 1102 2 0.598069557561292 1102 3 0.6909782971496234 1102 4 0.5056587117751548 1102 5 0.5220382928786478 1102 6 0.7154953084616977 1102 7 0.4734706211010149 1102 8 0.6459957082053265 1102 9 0.687154557112784 1102 10-11 0.7306855428752925 1102 12-13 0.7741422902056811 1102 14-15 0.8600383946206094 1102 16-17 1.129842100085753 1102 18-19 1.0347945960700358 1102 20-21 0.748347691450725 1102 22-23 0.696238984534304 1102 24-25 0.8828097063815576 1102 26-27 0.9829433679898614 1102 28-29 0.915347911078527 1102 30-31 0.7860693298067858 1102 32-33 0.8577546499993431 1102 34-35 1.0168759094541642 1102 36-37 1.2896789814141627 1102 38-39 1.3837258978193532 1102 40-41 1.4634251454684843 1102 42-43 1.7829355224103551 1102 44-45 1.824958138553967 1102 46-47 1.6004751960185715 1102 48-49 1.6670802403275395 1102 50-51 1.7779573219053013 1102 52-53 1.7188066634765065 1102 54-55 1.6082682958902978 1102 56-57 2.0450467565356014 1102 58-59 2.052738067156909 1102 60-61 2.13621370974721 1102 62-63 1.985879314983947 1102 64-65 2.195550443087008 1102 66-67 2.4390696915314045 1102 68-69 2.6801900739522964 1102 70-71 2.6928473145403125 1102 72-73 2.6330716951034177 1102 74-75 2.3854527699400805 1102 76-77 2.9161700294803943 1102 78-79 3.3648105081989854 1102 80-81 3.5528114428693343 1102 82-83 3.5903646093875032 1102 84-85 3.7845337860259 1102 86-87 4.006718506163036 1102 88-89 4.072119744910136 1102 90-91 4.014032192922441 1102 92-93 3.996945656665826 1102 94-95 3.697946747471775 1102 96-97 3.574545524610828 1102 98-99 2.89957867574412 1102 100-101 2.183006440405874 1103 1 0.602572929856894 1103 2 0.5681187032101498 1103 3 0.4606863447687459 1103 4 0.5078744723396369 1103 5 0.46518535813544304 1103 6 0.6564572049055641 1103 7 0.6375693651566579 1103 8 0.5330534088375103 1103 9 0.7675408085678086 1103 10-11 0.8734098301257234 1103 12-13 0.758949508635375 1103 14-15 0.8743778192815412 1103 16-17 0.9776031662694749 1103 18-19 1.0839249490529639 1103 20-21 1.0297864521176834 1103 22-23 1.1650849565948533 1103 24-25 1.1162971201701737 1103 26-27 1.061988171139589 1103 28-29 1.1529005097713423 1103 30-31 1.0330822747064516 1103 32-33 1.2370191019251777 1103 34-35 1.2032221989041432 1103 36-37 1.2631548621124438 1103 38-39 1.2761269067353211 1103 40-41 1.3900132015309268 1103 42-43 1.6044424414997636 1103 44-45 1.6715636013230366 1103 46-47 1.6221766786478113 1103 48-49 1.6144050681342144 1103 50-51 1.7924163603147782 1103 52-53 1.7359597644858837 1103 54-55 1.789780017884702 1103 56-57 2.184166264742604 1103 58-59 2.069800769540464 1103 60-61 2.2853477337403163 1103 62-63 2.5759738587921 1103 64-65 2.6530554410144163 1103 66-67 2.7151675912547013 1103 68-69 2.886439203999359 1103 70-71 2.7439782151155896 1103 72-73 3.018845459923206 1103 74-75 3.166474870950985 1103 76-77 3.2358932964959095 1103 78-79 3.4957393808470556 1103 80-81 3.752080147048627 1103 82-83 3.949701594356249 1103 84-85 4.0175291314909 1103 86-87 4.089097351355008 1103 88-89 4.152535670364159 1103 90-91 4.399374461086678 1103 92-93 4.485294788089519 1103 94-95 3.918175237264073 1103 96-97 3.805584527564461 1103 98-99 3.4933414764801576 1103 100-101 2.427332358808494 1104 1 0.6951570554118582 1104 2 0.5796655606430825 1104 3 0.5941418379157213 1104 4 0.5725382807949231 1104 5 0.5875113536734418 1104 6 0.6983754891346337 1104 7 0.6573385628292883 1104 8 0.6512556313958058 1104 9 0.8070926860422176 1104 10-11 0.7658431023791508 1104 12-13 0.9647282715855425 1104 14-15 0.8349169461788577 1104 16-17 0.9319765638011859 1104 18-19 1.0662021041126906 1104 20-21 1.0903206257979932 1104 22-23 1.1623416364036672 1104 24-25 1.2169667130450463 1104 26-27 1.1998454329074306 1104 28-29 1.4406852025136132 1104 30-31 1.447640013976141 1104 32-33 1.5850623174247005 1104 34-35 1.4625037655509914 1104 36-37 1.6410343224990172 1104 38-39 1.6533136494646428 1104 40-41 1.634075056367081 1104 42-43 1.698379029770777 1104 44-45 1.4295668569641364 1104 46-47 1.467319624387521 1104 48-49 1.501768473649701 1104 50-51 1.6740242155893945 1104 52-53 1.671927417927165 1104 54-55 2.026456596847183 1104 56-57 2.1543168308161746 1104 58-59 2.3034142673792246 1104 60-61 2.4852897605849975 1104 62-63 2.5907745987851385 1104 64-65 2.6570934852381427 1104 66-67 2.8038122972915254 1104 68-69 3.2024261661943 1104 70-71 3.219541123316361 1104 72-73 3.386412703507766 1104 74-75 3.3216745471454168 1104 76-77 3.4423986289710733 1104 78-79 3.7022452995017474 1104 80-81 3.640096849648099 1104 82-83 3.977261557591067 1104 84-85 4.009446301978873 1104 86-87 4.063013534716653 1104 88-89 3.800271376083277 1104 90-91 3.5522074374366763 1104 92-93 3.6249642238682753 1104 94-95 3.425022986510541 1104 96-97 3.4592340519970115 1104 98-99 3.4158163030003266 1104 100-101 2.45259816191548 1105 1 0.546976482603938 1105 2 0.5786502405419469 1105 3 0.609959688168118 1105 4 0.5636261489310499 1105 5 0.3572594397620179 1105 6 0.6121140254879407 1105 7 0.5091282572201408 1105 8 0.5287553891285341 1105 9 0.5195610811757305 1105 10-11 0.6553279320432353 1105 12-13 0.7430014125146158 1105 14-15 0.8836941571980574 1105 16-17 0.8482308255026965 1105 18-19 0.8112411311673355 1105 20-21 0.9424891381007612 1105 22-23 0.7846161296245882 1105 24-25 0.9487261901001318 1105 26-27 0.8538343634956576 1105 28-29 0.7474324560837573 1105 30-31 0.9264889801556322 1105 32-33 0.930178099155107 1105 34-35 1.0213879265574093 1105 36-37 1.1377229694142912 1105 38-39 1.3337174209052947 1105 40-41 1.3182202052953826 1105 42-43 1.485401873371842 1105 44-45 1.5321163896085466 1105 46-47 1.8702772672591337 1105 48-49 1.7592264512906262 1105 50-51 1.6610221717346114 1105 52-53 1.8769070014036586 1105 54-55 2.077907006939597 1105 56-57 2.1693368509023365 1105 58-59 2.272764704781764 1105 60-61 2.446340892193387 1105 62-63 2.3830810712855985 1105 64-65 2.447288928851542 1105 66-67 2.6829870028266747 1105 68-69 3.066524139077984 1105 70-71 2.9052488961313294 1105 72-73 3.0349357541372193 1105 74-75 3.1361457217452333 1105 76-77 3.2104574514640483 1105 78-79 3.5653031749499426 1105 80-81 3.7321165657989575 1105 82-83 3.8690550842096307 1105 84-85 3.984258214480743 1105 86-87 3.851949739137371 1105 88-89 3.8480437285425104 1105 90-91 3.5656527202727037 1105 92-93 3.670159182947131 1105 94-95 3.572216870803693 1105 96-97 3.0791816782182586 1105 98-99 2.3453726896060303 1105 100-101 1.4525717327588339 1106 1 0.501827926754256 1106 2 0.3694041261697798 1106 3 0.1604755249236014 1106 4 0.29263717827200963 1106 5 0.3351095421589818 1106 6 -0.04924706238085719 1106 7 0.38635515049404034 1106 8 0.16790050820647195 1106 9 0.437855363851277 1106 10-11 0.4775029473974328 1106 12-13 0.5767129387537082 1106 14-15 0.617370828879352 1106 16-17 0.29475261882988235 1106 18-19 0.7034089402146506 1106 20-21 0.6745011903414095 1106 22-23 0.6866745547442648 1106 24-25 0.7240788015972655 1106 26-27 0.6364691166194589 1106 28-29 0.9440430576472068 1106 30-31 0.5895435768913373 1106 32-33 0.6883908547997777 1106 34-35 0.5169961987771288 1106 36-37 0.5944998403256392 1106 38-39 0.6205767176296675 1106 40-41 0.924644839202152 1106 42-43 0.56730633924915 1106 44-45 0.9197779831493023 1106 46-47 1.014639335228459 1106 48-49 0.9407698795881423 1106 50-51 0.8066518970282708 1106 52-53 0.9398494376168749 1106 54-55 0.7742624185711264 1106 56-57 1.3585835912120707 1106 58-59 1.4165574103086342 1106 60-61 1.6831408318465861 1106 62-63 1.7577207559414632 1106 64-65 1.7223312465111107 1106 66-67 1.939480703861772 1106 68-69 2.078445307323584 1106 70-71 1.7223419136640032 1106 72-73 2.002356773655773 1106 74-75 2.3885262907171345 1106 76-77 2.3729797337715226 1106 78-79 2.80963028136361 1106 80-81 2.7371275437809324 1106 82-83 3.304800185311926 1106 84-85 3.0811568275742864 1106 86-87 2.556933726905374 1106 88-89 2.3689956511873262 1106 90-91 2.645504565665055 1106 92-93 2.940871831593931 1106 94-95 2.935588876735654 1106 96-97 2.3558038195025315 1106 98-99 2.518694534934184 1106 100-101 1.54268908802816 1107 1 0.31214639445860826 1107 2 0.2770814347669237 1107 3 0.2763200461387747 1107 4 0.22087224428463514 1107 5 0.29343433886961634 1107 6 0.2126719599357756 1107 7 0.22743214913001708 1107 8 0.33960371685147095 1107 9 0.21093243889165336 1107 10-11 0.37153042524865043 1107 12-13 0.2363995917146795 1107 14-15 0.38437493090645347 1107 16-17 0.4563276643127523 1107 18-19 0.3901016733442546 1107 20-21 0.5593411688958412 1107 22-23 0.54862264736839 1107 24-25 0.4716298702254349 1107 26-27 0.44023433633839204 1107 28-29 0.6371885617318327 1107 30-31 0.655468414316104 1107 32-33 0.8294910887490303 1107 34-35 0.6951728494328862 1107 36-37 0.76440924615315 1107 38-39 0.9048611681027339 1107 40-41 0.6649692805799461 1107 42-43 0.8600824070857982 1107 44-45 0.5884127174830596 1107 46-47 0.765876000762141 1107 48-49 0.7881248610116636 1107 50-51 0.8858351234346813 1107 52-53 0.9593502237711391 1107 54-55 0.9738308644923706 1107 56-57 1.144145460597688 1107 58-59 1.221729439282651 1107 60-61 1.215662813159426 1107 62-63 1.4039211149898492 1107 64-65 1.5005476954906243 1107 66-67 1.678221323716084 1107 68-69 1.752306678850747 1107 70-71 1.913148186884598 1107 72-73 1.9180897758335576 1107 74-75 2.281237493304207 1107 76-77 2.323926402709386 1107 78-79 2.4516212023219417 1107 80-81 2.3627516984590464 1107 82-83 2.4707164141577813 1107 84-85 2.2297780550739112 1107 86-87 2.2459560095184763 1107 88-89 2.7311860399837222 1107 90-91 2.4944063438729973 1107 92-93 2.598285584230471 1107 94-95 2.5758775755031618 1107 96-97 2.2329533803472614 1107 98-99 2.0186836760884503 1107 100-101 1.3310466522870144 1108 1 0.298171750313287 1108 2 0.2620351774644689 1108 3 0.16405671380118747 1108 4 0.10796279958938015 1108 5 0.04761366761652397 1108 6 0.1966147326986345 1108 7 0.21668132863720047 1108 8 0.2886601657091461 1108 9 0.34924066268661136 1108 10-11 0.3192246476465641 1108 12-13 0.1090971145513393 1108 14-15 0.3110027459810638 1108 16-17 0.41893263261481195 1108 18-19 0.4393950051103488 1108 20-21 0.39520092882354874 1108 22-23 0.452531215357304 1108 24-25 0.4797781481450869 1108 26-27 0.49853867697062526 1108 28-29 0.4189852631897466 1108 30-31 0.345556217217144 1108 32-33 0.4749317540975184 1108 34-35 0.6314745062905729 1108 36-37 0.4914316414705908 1108 38-39 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-1.0872524849793734 2312 62-63 -0.8737031206372698 2312 64-65 -0.8757407234257641 2312 66-67 -1.132788316060676 2312 68-69 -1.0920449727287362 2312 70-71 -1.1241425284883135 2312 72-73 -1.2896327267699803 2312 74-75 -1.4618130554420894 2312 76-77 -1.2768406404693344 2312 78-79 -1.3886649201379342 2312 80-81 -1.4662123593262102 2312 82-83 -1.3542388583461573 2312 84-85 -1.3875114586647754 2312 86-87 -1.3232881625555422 2312 88-89 -1.2516773208536875 2312 90-91 -1.060111133229194 2312 92-93 -1.2696748419491328 2312 94-95 -1.1582141225395102 2312 96-97 -1.2055329610770755 2312 98-99 -1.2355988147657797 2312 100-101 -0.6214821201768697 2313 1 -0.43190067403355314 2313 2 -0.352402918693695 2313 3 -0.24361892307130972 2313 4 -0.2621900096368961 2313 5 -0.07171373553052973 2313 6 -0.1612943039286634 2313 7 -0.27972399710019147 2313 8 -0.14040058470436634 2313 9 -0.4483150305528696 2313 10-11 -0.27252557193165217 2313 12-13 -0.3262920081903147 2313 14-15 -0.5402118151219426 2313 16-17 -0.5447314458226202 2313 18-19 -0.4297397215832106 2313 20-21 -0.508922598364336 2313 22-23 -0.44918526103215584 2313 24-25 -0.5539818990681127 2313 26-27 -0.7145258506904923 2313 28-29 -0.5986405372574737 2313 30-31 -0.3012915768736022 2313 32-33 -0.4688798942970678 2313 34-35 -0.38328380462262146 2313 36-37 -0.4140048116470325 2313 38-39 -0.6187993621873531 2313 40-41 -0.6753008048370148 2313 42-43 -0.5153341519803121 2313 44-45 -0.7168480310419199 2313 46-47 -0.7232203937990498 2313 48-49 -0.5716552712759722 2313 50-51 -0.7153419588426821 2313 52-53 -0.6286900944354699 2313 54-55 -0.9555894589283902 2313 56-57 -0.7800830279850608 2313 58-59 -0.7942122154400479 2313 60-61 -1.0653263325649007 2313 62-63 -0.9712080981732072 2313 64-65 -0.8699599349613294 2313 66-67 -0.9656174991572399 2313 68-69 -0.8848457405154306 2313 70-71 -1.0078728801330996 2313 72-73 -1.214899087724497 2313 74-75 -1.275411086602741 2313 76-77 -1.1116417664680434 2313 78-79 -1.4228842359150917 2313 80-81 -1.2649930407061056 2313 82-83 -1.4672971549817149 2313 84-85 -1.8508128514271895 2313 86-87 -1.632610748580916 2313 88-89 -1.53065316618191 2313 90-91 -1.5565056772243562 2313 92-93 -1.9068892318373187 2313 94-95 -1.768499829785533 2313 96-97 -1.4749009301002651 2313 98-99 -1.0830240711763999 2313 100-101 -0.7168875773188876 2314 1 -0.49104506391445923 2314 2 -0.5496852644992458 2314 3 -0.286524423838145 2314 4 -0.3188942095354008 2314 5 -0.36647989936207637 2314 6 -0.37202050922451235 2314 7 -0.49056670241509437 2314 8 -0.4372647197123527 2314 9 -0.3112860091845846 2314 10-11 -0.47918386354139386 2314 12-13 -0.6012776823285932 2314 14-15 -0.6909016772660195 2314 16-17 -0.5902055293012012 2314 18-19 -0.6528353164019691 2314 20-21 -0.6744557764775791 2314 22-23 -0.7070851151886899 2314 24-25 -0.6836364692303718 2314 26-27 -0.7776883990410397 2314 28-29 -0.6239152163909338 2314 30-31 -0.7407730935388059 2314 32-33 -0.6390094835786826 2314 34-35 -0.6288488551994078 2314 36-37 -0.50945279184009 2314 38-39 -0.6289743039555447 2314 40-41 -0.6086257809120283 2314 42-43 -0.7903455532061372 2314 44-45 -0.46851052272005234 2314 46-47 -0.6404405771361006 2314 48-49 -0.6284895628624163 2314 50-51 -0.5065581486561648 2314 52-53 -0.4056961944250048 2314 54-55 -0.5229423673392333 2314 56-57 -0.19474397288273693 2314 58-59 -0.4037181073071565 2314 60-61 -0.2783161575017772 2314 62-63 -0.36218847253145725 2314 64-65 -0.29028520786869194 2314 66-67 -0.4116847861703299 2314 68-69 -0.45607215320558225 2314 70-71 -0.5978486359937207 2314 72-73 -0.8489565091276319 2314 74-75 -0.6663576031429521 2314 76-77 -0.8553618693472664 2314 78-79 -0.8917036125865927 2314 80-81 -0.6319636106169177 2314 82-83 -0.6345199474337733 2314 84-85 -0.5946470272417343 2314 86-87 -0.4884353005230544 2314 88-89 -0.6633286061090384 2314 90-91 -0.8218589841623452 2314 92-93 -0.6567192752991069 2314 94-95 -0.8027945228941427 2314 96-97 -0.613130993852474 2314 98-99 -0.6675475047911696 2314 100-101 -0.202340608273186 2315 1 -0.7721412855869154 2315 2 -0.6224936697573646 2315 3 -0.5040864183154135 2315 4 -0.6732762840662829 2315 5 -0.513506753621634 2315 6 -0.46571074310445937 2315 7 -0.659333899014733 2315 8 -0.7335812870449772 2315 9 -1.292854103692946 2315 10-11 -0.8844117537160585 2315 12-13 -0.9022817593396297 2315 14-15 -0.7988179666037212 2315 16-17 -0.7905870909537498 2315 18-19 -0.9408245484348186 2315 20-21 -0.7509099537724282 2315 22-23 -0.885745164816484 2315 24-25 -0.8652797611163692 2315 26-27 -0.7640765587369742 2315 28-29 -0.8686364719765365 2315 30-31 -1.0248907136347185 2315 32-33 -0.9716859031924301 2315 34-35 -0.8582806380462316 2315 36-37 -1.1002621113595694 2315 38-39 -1.1605232584394543 2315 40-41 -1.1915207932322645 2315 42-43 -1.1216718887648298 2315 44-45 -1.4124675400476612 2315 46-47 -1.0655057089668922 2315 48-49 -1.1340690348455063 2315 50-51 -1.0895433482760915 2315 52-53 -1.0671738290896329 2315 54-55 -1.0348480819682777 2315 56-57 -0.7705413190471155 2315 58-59 -0.727649332058121 2315 60-61 -0.9072800317896039 2315 62-63 -0.6473005067134352 2315 64-65 -0.5161347709929878 2315 66-67 -0.603627393351367 2315 68-69 -0.6013974654853023 2315 70-71 -0.8195933142616774 2315 72-73 -0.9935932760616026 2315 74-75 -1.031378062528315 2315 76-77 -0.8455375239794627 2315 78-79 -0.8009827459956611 2315 80-81 -0.8562968378232547 2315 82-83 -1.1100945507892845 2315 84-85 -1.0011188331804668 2315 86-87 -0.9083767283317172 2315 88-89 -1.0106775578675062 2315 90-91 -0.9262061854447889 2315 92-93 -0.9429999039838712 2315 94-95 -0.9292578056375227 2315 96-97 -0.8307114469941013 2315 98-99 -0.493892726412831 2315 100-101 -0.03754029013996352 2316 1 -1.0277934309207524 2316 2 -0.9023615480871108 2316 3 -0.70376433066156 2316 4 -0.6008098485577591 2316 5 -0.7898809668896689 2316 6 -0.6816047828395639 2316 7 -0.7599402783222473 2316 8 -0.7901635426445353 2316 9 -0.8233258963736674 2316 10-11 -0.9211363194728861 2316 12-13 -0.8335357648940018 2316 14-15 -1.0521661992298874 2316 16-17 -0.8062543546876668 2316 18-19 -1.0108786131481438 2316 20-21 -0.9581963652887069 2316 22-23 -0.9154396745366284 2316 24-25 -0.8458690500585746 2316 26-27 -1.1692283178942944 2316 28-29 -0.9511977261464253 2316 30-31 -0.8362952458681363 2316 32-33 -1.2329252833380266 2316 34-35 -0.9940126479717861 2316 36-37 -1.2582543220969242 2316 38-39 -1.1838713267351793 2316 40-41 -1.2380476694750158 2316 42-43 -0.8009202413522161 2316 44-45 -1.0389411732292615 2316 46-47 -0.9549146123898922 2316 48-49 -0.674180450880872 2316 50-51 -0.5578144277627253 2316 52-53 -0.8008780014393935 2316 54-55 -0.6521832996239425 2316 56-57 -0.7141332537093312 2316 58-59 -0.5307831134657732 2316 60-61 -0.7586825920577915 2316 62-63 -0.6003430847293068 2316 64-65 -0.6202446751883457 2316 66-67 -0.6700234625673467 2316 68-69 -0.5855839476636646 2316 70-71 -0.48642814574081683 2316 72-73 -0.37350552360896927 2316 74-75 -0.24282860728370892 2316 76-77 -0.18198108469134056 2316 78-79 -0.17469316290197767 2316 80-81 -0.30154850988543913 2316 82-83 -0.2627542388901176 2316 84-85 -0.14492604071337567 2316 86-87 0.018750939983736004 2316 88-89 -0.19931410824300144 2316 90-91 -0.39053004786734924 2316 92-93 -0.12105028769543935 2316 94-95 -0.25079330922814336 2316 96-97 -0.2891802159948096 2316 98-99 -0.41311445746963926 2316 100-101 -0.10032437638057612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8393.0 3 3439.0 4 3825.0 5 4303.0 6 5200.0 7 6639.0 8 8261.0 9 10492.0 10 13144.0 11 15992.0 12 19036.0 13 22348.0 14 25134.0 15 28271.0 16 31906.0 17 36054.0 18 36397.0 19 36678.0 20 39214.0 21 43422.0 22 47222.0 23 52343.0 24 57166.0 25 62225.0 26 67791.0 27 74047.0 28 80225.0 29 87107.0 30 94556.0 31 103436.0 32 113139.0 33 123788.0 34 135182.0 35 139640.0 36 131100.0 37 86241.0 38 19892.0 39 227.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27476252688754 18.30394414893902 13.893905276549134 23.527388047624303 2 15.630440992346237 19.55801989924904 40.16482152343781 24.64671758496691 3 19.491258899018003 23.889014368179733 29.13960185154038 27.480124881261887 4 12.933529059643304 15.816665180385575 36.40829761363776 34.84150814633336 5 13.908847797837867 37.1778527056554 33.84968052986697 15.063618966639766 6 32.98188741518488 35.30744397061611 17.15881022507884 14.551858389120165 7 29.47524791069395 32.20737834341031 20.054935226306114 18.26243851958963 8 26.743378860840473 34.93443205956702 19.372525649939075 18.949663429653434 9 26.948732242748118 16.427820815952533 19.7916332933922 36.83181364790715 10-11 25.39917741618976 26.31950479440077 26.891522260623706 21.38979552878576 12-13 26.282830445520432 23.55937945415651 27.37394611937266 22.783843980950394 14-15 22.90891092967933 25.070697616556632 25.69020441909157 26.330187034672463 16-17 22.537261478222383 28.30478893482118 26.429635775290212 22.728313811666233 18-19 22.564012756367372 26.674406697039476 28.827110430781012 21.93447011581214 20-21 23.054330649590096 25.89440680215045 29.03517931119363 22.016083237065825 22-23 22.771241048335252 25.878694130185455 28.782071681663528 22.56799313981577 24-25 22.537350612900617 26.056025719104593 28.98635040918335 22.42027325881144 26-27 22.14390217855617 26.420733660828706 28.845899188381036 22.58946497223409 28-29 22.20594607192599 26.769177047050714 28.74177705126881 22.283099829754494 30-31 22.321913895014497 26.367796329618816 28.71627430145822 22.59401547390847 32-33 21.955328768644602 26.408062669787626 28.64899237984589 22.987616181721883 34-35 22.67572176683909 26.468575004378224 28.570177649254845 22.285525579527835 36-37 22.748642583344502 26.100283043197592 28.447309701371005 22.7037646720869 38-39 22.2315497822556 26.469284226176576 28.496529302403793 22.80263668916403 40-41 22.723660816406387 26.33703488882655 28.409934436477478 22.529369858289584 42-43 22.343511022493416 26.29860960968175 28.440435294400267 22.917444073424566 44-45 22.39407281819793 26.544546749317238 28.412592325283942 22.648788107200893 46-47 22.714757011039723 26.812083394395223 27.739797058298294 22.73336253626676 48-49 22.59536947664238 26.692402473712534 27.81338354502823 22.898844504616854 50-51 22.78130641956565 26.952897703553518 27.632281360336787 22.633514516544047 52-53 22.956998000838098 26.793734002033858 27.559848074372024 22.689419922756013 54-55 22.505322136028884 26.912178883557857 27.77792012845725 22.804578851956006 56-57 22.486465047205517 27.038196805847225 27.599319488944097 22.876018658003158 58-59 22.62101122036106 26.507942862633314 27.665663132946865 23.20538278405876 60-61 22.35777815204014 26.859691738893815 27.84297564557366 22.93955446349238 62-63 22.437883556758926 26.852096029586985 27.860660795961685 22.849359617692404 64-65 22.190949907891248 26.71474151096791 27.805313428660956 23.28899515247989 66-67 22.23019048203672 26.74803366063398 27.70658239942778 23.315193457901522 68-69 22.626036648901643 26.45040099415804 27.81373842711216 23.109823929828156 70-71 22.35048614125082 26.624274444926684 27.555264434895825 23.469974978926672 72-73 22.499416789937076 26.605696080561465 27.46994132666543 23.424945802836028 74-75 22.310506643975124 26.892411190566495 27.469326516618146 23.32775564884023 76-77 22.439653883930475 26.627636973139175 27.470427467224667 23.462281675705686 78-79 22.58527041779102 26.63023373129309 27.35229981236371 23.432196038552185 80-81 22.564873320163958 26.69406238397724 27.23639829593514 23.504665999923663 82-83 22.553062913708423 26.628442026035852 27.16350869850357 23.654986361752155 84-85 22.56615036075221 26.471514561406877 27.217334255765735 23.745000822075173 86-87 22.608943514072042 26.481580281128537 27.065914705963205 23.843561498836216 88-89 22.576203830197734 26.452606735010814 26.997859774382103 23.973329660409348 90-91 22.553328033409805 26.436171604333836 26.946880991445198 24.06361937081116 92-93 22.688697463481375 26.021529886590542 27.13096151007617 24.15881113985191 94-95 22.434363209549225 25.830333361273162 27.25884148669059 24.476461942487028 96-97 22.555550632076795 25.733632443565813 27.049438073878164 24.661378850479228 98-99 22.404837155968814 25.709816017567334 26.73642779565669 25.14891903080716 100-101 23.29228571451476 24.810120358427135 27.00375363519006 24.89384029186805 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 258.0 1 197.0 2 181.0 3 237.5 4 223.0 5 229.5 6 394.0 7 731.5 8 1158.0 9 1416.0 10 1340.0 11 1223.0 12 1278.0 13 1397.0 14 1498.5 15 1623.0 16 1823.5 17 2089.5 18 2328.5 19 2508.5 20 2839.0 21 3378.5 22 3981.5 23 4739.0 24 5839.5 25 7262.5 26 9172.0 27 11650.5 28 14024.5 29 16665.5 30 19832.5 31 23486.0 32 28109.5 33 31569.5 34 33835.5 35 36954.5 36 40953.5 37 44742.0 38 47462.0 39 49455.0 40 52357.0 41 56256.5 42 59540.0 43 62532.0 44 65178.5 45 67510.5 46 68677.5 47 69681.0 48 70919.5 49 70438.5 50 68969.5 51 66898.5 52 64783.5 53 63948.5 54 63112.5 55 59979.5 56 55450.0 57 51655.0 58 48497.5 59 43831.5 60 37278.0 61 31255.0 62 26418.0 63 21044.5 64 16172.5 65 12808.0 66 9774.0 67 7364.5 68 5645.0 69 4275.5 70 3345.0 71 2537.0 72 1923.0 73 1571.0 74 1229.5 75 936.5 76 679.0 77 432.5 78 248.0 79 139.0 80 83.5 81 52.5 82 30.5 83 20.0 84 13.0 85 7.0 86 5.0 87 4.0 88 2.5 89 1.5 90 1.5 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3933332443720893 2 0.028823443067027846 3 0.035015145651796795 4 0.04227438316497418 5 0.06170351886200776 6 0.054444281348830385 7 0.03864476440838549 8 0.0406197040259411 9 0.03837788067628338 10-11 0.038297815556652744 12-13 0.03071831756495283 14-15 0.028476494215295106 16-17 0.02284524746794059 18-19 0.028182922109982782 20-21 0.034988457278586585 22-23 0.03410774096264962 24-25 0.04171392732755975 26-27 0.03602930383378481 28-29 0.03157234550767958 30-31 0.034428001441172154 32-33 0.029197080291970805 34-35 0.03047812220606093 36-37 0.03288007579497992 38-39 0.015612698327973419 40-41 0.013531005217576962 42-43 0.013877954069309705 44-45 0.011048986509027343 46-47 0.02004296828086844 48-49 0.01809471703652304 50-51 0.012436781915958314 52-53 0.01014158181988017 54-55 0.008913916652210465 56-57 0.009447684116414683 58-59 0.005070790909940085 60-61 0.008566967800477722 62-63 0.011689507466072405 64-65 0.007793004977381603 66-67 0.0037363722494295364 68-69 0.0069923537810752745 70-71 0.013557693590787174 72-73 0.011849637705333671 74-75 0.01262360052842979 76-77 0.006138325838348524 78-79 0.00907404689147173 80-81 0.010114893446669958 82-83 0.013370874978315697 84-85 0.008913916652210465 86-87 0.0032826699048559495 88-89 0.01644003789748996 90-91 0.026261359238847596 92-93 0.011662819092862195 94-95 0.021457452061009623 96-97 0.019429135697033587 98-99 0.012036456317805148 100-101 0.1257289261933039 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1873475.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.35769173610737 #Duplication Level Percentage of deduplicated Percentage of total 1 81.3932871814917 49.94104224270427 2 10.8499800392622 13.314594611839361 3 3.346203678700922 6.159460014118788 4 1.4233013866962563 3.493219509299321 5 0.7687666883714546 2.3584874741041912 6 0.45869224434465117 1.6886578398145404 7 0.30640359838762915 1.3160152275691535 8 0.23405970672083729 1.1489090666256665 9 0.16975301384805874 0.9374087785467919 >10 0.9446220968150416 10.557292747519616 >50 0.06769556024686885 2.86144672334718 >100 0.03346846191557199 3.7119563115495726 >500 0.0025400919247486934 1.0643765453202063 >1k 0.0011386618973011384 1.1483228297832286 >5k 8.758937671547216E-5 0.29881007785813407 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5560 0.29677471009754597 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4390 0.23432391678565234 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2761 0.14737319686678496 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 8.006511963063291E-5 12-13 0.0 0.0 0.0 0.0 1.0675349284084389E-4 14-15 0.0 0.0 0.0 0.0 1.0675349284084389E-4 16-17 0.0 0.0 0.0 2.668837321021097E-5 1.6013023926126583E-4 18-19 0.0 0.0 0.0 5.337674642042194E-5 1.6013023926126583E-4 20-21 0.0 0.0 0.0 1.0675349284084389E-4 2.1350698568168777E-4 22-23 0.0 0.0 0.0 2.1350698568168777E-4 2.668837321021097E-4 24-25 0.0 0.0 0.0 3.2026047852253166E-4 2.668837321021097E-4 26-27 0.0 0.0 0.0 0.0012276651676697047 2.668837321021097E-4 28-29 0.0 0.0 0.0 0.003602930383378481 2.668837321021097E-4 30-31 0.0 0.0 0.0 0.013103991246213587 2.668837321021097E-4 32-33 0.0 0.0 0.0 0.0316257222541 2.668837321021097E-4 34-35 0.0 0.0 0.0 0.060716049053229956 2.668837321021097E-4 36-37 0.0 0.0 0.0 0.11500020016279908 2.668837321021097E-4 38-39 0.0 0.0 0.0 0.19834798969828793 2.668837321021097E-4 40-41 0.0 0.0 0.0 0.286019295693831 2.668837321021097E-4 42-43 0.0 0.0 0.0 0.3728632621198575 2.668837321021097E-4 44-45 0.0 0.0 0.0 0.4856483273062091 2.935721053123207E-4 46-47 0.0 0.0 0.0 0.6201043515392519 3.2026047852253166E-4 48-49 0.0 0.0 0.0 0.7648087110850158 3.2026047852253166E-4 50-51 0.0 0.0 0.0 0.9212826432164827 3.736372249429536E-4 52-53 0.0 0.0 0.0 1.068442333097586 3.736372249429536E-4 54-55 0.0 0.0 0.0 1.2328427120724856 3.736372249429536E-4 56-57 0.0 0.0 0.0 1.4243584782289596 3.736372249429536E-4 58-59 0.0 0.0 0.0 1.6339422730487463 4.2701397136337554E-4 60-61 0.0 0.0 0.0 1.86463657107781 4.2701397136337554E-4 62-63 0.0 0.0 0.0 2.088738840923951 4.2701397136337554E-4 64-65 0.0 0.0 0.0 2.328533874217697 4.2701397136337554E-4 66-67 0.0 0.0 0.0 2.601502555411735 4.2701397136337554E-4 68-69 0.0 0.0 0.0 2.9076982612524853 4.2701397136337554E-4 70-71 0.0 0.0 0.0 3.2344173260918883 4.537023445735865E-4 72-73 0.0 0.0 0.0 3.5551581953322033 4.803907177837975E-4 74-75 0.0 0.0 0.0 3.867679045623774 4.803907177837975E-4 76-77 0.0 0.0 0.0 4.210464511135724 4.803907177837975E-4 78-79 0.0 0.0 0.0 4.584635503542881 4.803907177837975E-4 80-81 0.0 0.0 0.0 4.986322208729767 4.803907177837975E-4 82-83 0.0 0.0 0.0 5.405089472771187 5.604558374144304E-4 84-85 0.0 0.0 0.0 5.808857871068469 5.871442106246414E-4 86-87 0.0 0.0 0.0 6.225596818745913 6.138325838348523E-4 88-89 0.0 0.0 0.0 6.665554651116241 6.405209570450633E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3895 0.0 47.628925 1 GTATCAA 6700 0.0 40.92811 1 ATCAACG 8165 0.0 32.939735 3 TCAACGC 8340 0.0 32.191578 4 CAACGCA 8430 0.0 31.847895 5 TATCAAC 8670 0.0 31.240332 2 AACGCAG 8755 0.0 30.769272 6 GTACATG 7480 0.0 27.160429 1 TACATGG 7445 0.0 26.93442 2 ACGCAGA 10010 0.0 26.76348 7 CGCAGAG 10170 0.0 26.249006 8 ACATGGG 7710 0.0 25.145811 3 GCAGAGT 11050 0.0 23.59913 9 GAGTACT 6205 0.0 22.543919 12-13 GTACTTT 6555 0.0 21.16074 14-15 GTAAGGT 850 0.0 20.68439 4 TAAGGTG 1020 0.0 20.498043 5 CATGGGG 4165 0.0 20.421982 4 CAGAGTA 10710 0.0 20.356783 10-11 TACCTGG 1405 0.0 20.292465 2 >>END_MODULE