FastQCFastQC Report
Thu 26 May 2016
SRR938444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873475
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55530.29640107287260303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47260.25225850358291413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31850.17000493734904387No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38850.054.670631
GTATCAA66900.038.708651
ATCAACG85600.030.0191783
TCAACGC86950.029.498474
CAACGCA88100.029.1134175
TATCAAC90400.028.6384642
AACGCAG91400.028.1662086
ACGCAGA104350.024.6707387
CGCAGAG104900.024.4961098
GTACATG73450.024.4533081
TACATGG75250.023.6094992
GAGTACT65400.023.09535612-13
ACATGGG77450.022.1391583
GCAGAGT114350.021.8486639
TAAGGTG10700.021.751425
TATACCG1100.002878739321.595
GTACTTT69700.021.5001714-15
GGACCGA7850.020.572386
GCACCGT3252.1827873E-1120.4606786
AGTACTT68500.020.28199212-13
Produced by FastQC (version 0.11.5)