##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938444_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1873475 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.792457865730796 31.0 31.0 34.0 30.0 34.0 2 32.052589973178186 31.0 31.0 34.0 30.0 34.0 3 32.10789415391185 33.0 31.0 34.0 30.0 34.0 4 35.76770012943861 37.0 35.0 37.0 35.0 37.0 5 35.661214054097336 37.0 35.0 37.0 33.0 37.0 6 35.69324704092662 37.0 35.0 37.0 33.0 37.0 7 35.611580618903375 37.0 35.0 37.0 33.0 37.0 8 35.581152137071484 37.0 35.0 37.0 33.0 37.0 9 37.11766423357664 39.0 37.0 39.0 33.0 39.0 10-11 37.049608348123144 39.0 37.0 39.0 33.0 39.0 12-13 36.93333618009314 39.0 37.0 39.0 33.0 39.0 14-15 37.94464537824097 40.0 37.0 41.0 33.0 41.0 16-17 38.032069549900584 40.0 37.0 41.0 33.0 41.0 18-19 38.16978395761886 40.0 37.5 41.0 33.0 41.0 20-21 38.11973258250043 40.0 37.0 41.0 33.0 41.0 22-23 37.99689187205594 40.0 37.0 41.0 33.0 41.0 24-25 37.88304621091821 40.0 37.0 41.0 32.5 41.0 26-27 37.67191555798716 40.0 37.0 41.0 32.0 41.0 28-29 37.52691308931264 39.5 36.5 41.0 32.0 41.0 30-31 37.26336433632688 39.0 36.0 41.0 31.0 41.0 32-33 37.14613512323356 39.0 36.0 41.0 31.0 41.0 34-35 37.012199255394385 39.0 36.0 41.0 31.0 41.0 36-37 36.88469848810365 39.0 35.5 41.0 30.5 41.0 38-39 36.67462255968188 39.0 35.0 40.5 30.0 41.0 40-41 36.44785492200323 39.0 35.0 40.0 30.0 41.0 42-43 36.18202324557306 38.5 35.0 40.0 30.0 41.0 44-45 35.88363815903602 38.0 35.0 40.0 29.0 41.0 46-47 35.45821454783224 38.0 34.5 40.0 27.5 41.0 48-49 35.04346014758671 38.0 34.0 40.0 26.0 41.0 50-51 34.64791763968027 38.0 33.5 40.0 25.0 41.0 52-53 34.04761846301659 37.5 33.0 40.0 23.5 41.0 54-55 33.623614673267596 37.0 32.5 40.0 23.0 41.0 56-57 33.236292451193634 37.0 32.0 39.0 22.0 40.5 58-59 32.711549660390446 36.0 31.0 39.0 19.5 40.0 60-61 32.22655866771641 36.0 31.0 39.0 18.0 40.0 62-63 31.723012850451703 35.0 30.0 38.5 14.5 40.0 64-65 31.075880916478734 35.0 29.0 38.0 10.0 40.0 66-67 30.441406477268178 34.0 28.5 38.0 7.5 40.0 68-69 29.72444735051175 34.0 28.0 37.0 6.0 39.0 70-71 28.962574360479856 33.5 26.5 36.0 2.0 39.0 72-73 28.38765369166922 33.0 26.0 36.0 2.0 39.0 74-75 27.676990085269352 32.5 25.5 35.0 2.0 38.0 76-77 26.54377720546044 31.0 24.5 34.0 2.0 36.0 78-79 27.041506291783982 32.0 25.0 35.0 2.0 37.0 80-81 26.65761966399338 32.0 24.5 35.0 2.0 36.0 82-83 25.99097826232002 31.5 22.5 34.5 2.0 36.0 84-85 25.215598820373906 31.0 20.0 34.0 2.0 35.5 86-87 24.320104885306716 31.0 16.5 34.0 2.0 35.0 88-89 23.357840109956097 30.0 7.5 34.0 2.0 35.0 90-91 22.512707935787773 29.0 2.0 34.0 2.0 35.0 92-93 21.447991299590335 29.0 2.0 34.0 2.0 35.0 94-95 20.291076208649702 27.5 2.0 33.0 2.0 35.0 96-97 18.943179652784266 25.5 2.0 33.0 2.0 34.0 98-99 17.20722240755815 21.0 2.0 32.0 2.0 34.0 100-101 12.947343572772521 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19061470375089584 1101 2 0.20117278018365425 1101 3 0.21340667005095781 1101 4 0.21848927000768015 1101 5 0.1767526994252293 1101 6 0.18116715401694705 1101 7 0.2533806840002697 1101 8 0.2654659836737494 1101 9 0.207351150242161 1101 10-11 0.21275938620128443 1101 12-13 0.26062611804904634 1101 14-15 0.3905703089670425 1101 16-17 0.3426597234537567 1101 18-19 0.32559025357105753 1101 20-21 0.3279172333581286 1101 22-23 0.33133373859222814 1101 24-25 0.3381616797364515 1101 26-27 0.4569735590067978 1101 28-29 0.4264019310100835 1101 30-31 0.502615257182164 1101 32-33 0.38143680147197756 1101 34-35 0.2832932179950234 1101 36-37 0.399148802557896 1101 38-39 0.6011141041157657 1101 40-41 0.730337719577463 1101 42-43 0.6004631952515922 1101 44-45 0.5852334807238506 1101 46-47 0.5203677092241463 1101 48-49 0.5528077080974967 1101 50-51 0.6547890524252509 1101 52-53 0.8057884403576452 1101 54-55 0.9217861812122976 1101 56-57 0.8809834584914498 1101 58-59 0.9595628152145395 1101 60-61 0.9321639247253657 1101 62-63 1.036624441496599 1101 64-65 1.0776468956779617 1101 66-67 1.138068587303163 1101 68-69 1.3689212855935473 1101 70-71 1.4673613166454835 1101 72-73 1.5163395006476783 1101 74-75 1.2630506928871839 1101 76-77 1.2955108757866647 1101 78-79 1.5543153691810936 1101 80-81 1.5960056999015926 1101 82-83 1.6523423857655715 1101 84-85 1.9559833041635137 1101 86-87 1.8119125397263502 1101 88-89 1.5553200097357234 1101 90-91 1.9577475944369311 1101 92-93 2.501379782791968 1101 94-95 2.3315639814968385 1101 96-97 2.425256453284362 1101 98-99 2.3996571215823614 1101 100-101 2.175279666040181 1102 1 0.27144979289623095 1102 2 0.21396099946178282 1102 3 0.12068085021789443 1102 4 0.19961359066504514 1102 5 0.04698232754959264 1102 6 0.2044862727852177 1102 7 0.2212150229466019 1102 8 0.16728189308760477 1102 9 0.2265660234340814 1102 10-11 0.19151591988460837 1102 12-13 0.2612194017052616 1102 14-15 0.43529561542563044 1102 16-17 0.22493149717784178 1102 18-19 0.23506965212840925 1102 20-21 0.3194383038661428 1102 22-23 0.3757417001747072 1102 24-25 0.22951513959010583 1102 26-27 0.22632311660329663 1102 28-29 0.25203902720515003 1102 30-31 0.18476556283861356 1102 32-33 0.24112682107494265 1102 34-35 0.27246773934208335 1102 36-37 0.2912714073833129 1102 38-39 0.30075260292323946 1102 40-41 0.20318861332269478 1102 42-43 0.04360177732963422 1102 44-45 0.14137204905078704 1102 46-47 0.08479330277518216 1102 48-49 0.24182806431215198 1102 50-51 0.310372229021489 1102 52-53 0.34888714423988887 1102 54-55 0.055250863034714826 1102 56-57 0.2745447341659002 1102 58-59 0.4187400715029952 1102 60-61 0.5128512659192381 1102 62-63 0.5518385079571999 1102 64-65 0.627073856699699 1102 66-67 0.758033201134019 1102 68-69 1.156516263691099 1102 70-71 1.4196274218597509 1102 72-73 1.2942818163026963 1102 74-75 0.9957372151265353 1102 76-77 0.9811612952716686 1102 78-79 1.16475092769738 1102 80-81 1.3824500163419522 1102 82-83 1.160775783830946 1102 84-85 1.388279753476187 1102 86-87 1.374747894726326 1102 88-89 1.0301773420524931 1102 90-91 1.2896422059912176 1102 92-93 1.4255120500583907 1102 94-95 1.1265983057753282 1102 96-97 1.377021865595875 1102 98-99 1.1161599718802258 1102 100-101 1.1332604236647867 1103 1 0.27839870925991406 1103 2 0.23072589114403996 1103 3 0.17014740655031346 1103 4 0.1643657745337066 1103 5 0.07774172189409256 1103 6 0.2036810978845267 1103 7 0.20243382891994344 1103 8 0.27910178109119954 1103 9 0.31374027863965637 1103 10-11 0.3745699250331995 1103 12-13 0.4621129867679201 1103 14-15 0.5375511452671233 1103 16-17 0.3659894517215605 1103 18-19 0.3142857690868013 1103 20-21 0.2980574227931001 1103 22-23 0.3305960757894084 1103 24-25 0.24538048081814878 1103 26-27 0.32709724087617076 1103 28-29 0.3728092969173318 1103 30-31 0.330649672106226 1103 32-33 0.4052137873062378 1103 34-35 0.5569624630297412 1103 36-37 0.5863770330534592 1103 38-39 0.4678103341471669 1103 40-41 0.599953382637942 1103 42-43 0.5602043797699352 1103 44-45 0.6401520499991378 1103 46-47 0.599576460792278 1103 48-49 0.907678186143805 1103 50-51 0.7340722707792295 1103 52-53 0.7183772256292471 1103 54-55 0.8826370578949962 1103 56-57 0.989332175028224 1103 58-59 1.0422954142759266 1103 60-61 1.0960430869085442 1103 62-63 1.2412022148209232 1103 64-65 1.3262962143754002 1103 66-67 1.169616920815681 1103 68-69 1.1652505146750052 1103 70-71 1.3808539063627308 1103 72-73 1.4792309804595654 1103 74-75 1.3088125886135842 1103 76-77 1.065886221889702 1103 78-79 1.2449332851283437 1103 80-81 1.5225700277832672 1103 82-83 1.5531185154602163 1103 84-85 1.7617943327401804 1103 86-87 1.5271137843115206 1103 88-89 1.240178856344194 1103 90-91 1.297555680281146 1103 92-93 1.6509599515407096 1103 94-95 1.421712650094669 1103 96-97 1.6042924236353109 1103 98-99 1.7658619317407833 1103 100-101 1.5928190458734797 1104 1 0.20060535864600837 1104 2 0.270448639950871 1104 3 0.26842773166892187 1104 4 0.21988342381311554 1104 5 0.2383053804046611 1104 6 0.18438758489888585 1104 7 0.2403227150733258 1104 8 0.2271686196257079 1104 9 0.2727384861026536 1104 10-11 0.34140564587575284 1104 12-13 0.21023252284199145 1104 14-15 0.3052616804373045 1104 16-17 0.37762262408998737 1104 18-19 0.42645063559613305 1104 20-21 0.4209120057205027 1104 22-23 0.5040644766095923 1104 24-25 0.39771564420397 1104 26-27 0.5907651889312859 1104 28-29 0.44312021240079247 1104 30-31 0.4244589128747762 1104 32-33 0.6303039417176421 1104 34-35 0.6448470407635867 1104 36-37 0.639994581392763 1104 38-39 0.7758306769215082 1104 40-41 0.7540766866740256 1104 42-43 0.6278036287567232 1104 44-45 0.546755626163197 1104 46-47 0.6020448627848225 1104 48-49 0.8555940834498372 1104 50-51 0.7266267649590574 1104 52-53 0.8218238440181622 1104 54-55 0.9363385764516821 1104 56-57 1.0862418658654178 1104 58-59 1.057933218634986 1104 60-61 1.2542853279432222 1104 62-63 1.2335950699958858 1104 64-65 1.3314743777811522 1104 66-67 1.2743434622040617 1104 68-69 1.2577725051999984 1104 70-71 1.3441419221567514 1104 72-73 1.5382213038079158 1104 74-75 1.4689662145442917 1104 76-77 1.5587453828792235 1104 78-79 1.7393360033600516 1104 80-81 1.7950322807525616 1104 82-83 1.7262023825100101 1104 84-85 2.201740240093333 1104 86-87 1.9193193258183072 1104 88-89 1.7107511785881861 1104 90-91 2.436419705388385 1104 92-93 2.415082628364697 1104 94-95 2.333313992914732 1104 96-97 2.487325427887459 1104 98-99 2.4678681897934283 1104 100-101 1.9129041356937684 1105 1 0.17367074437954955 1105 2 0.13844491343978405 1105 3 0.1803129234124583 1105 4 0.09245964990574151 1105 5 0.10868248042140038 1105 6 0.09665820184350338 1105 7 0.11086169367452214 1105 8 0.14957922300335014 1105 9 0.3299829381821908 1105 10-11 0.2239181336522691 1105 12-13 0.25669387901011476 1105 14-15 0.3002010475470982 1105 16-17 0.2834621462508409 1105 18-19 0.2749752279263973 1105 20-21 0.23215827260212762 1105 22-23 0.22439114098573043 1105 24-25 0.208124437184388 1105 26-27 0.2155812860496269 1105 28-29 0.3269387410015412 1105 30-31 0.4534483940704348 1105 32-33 0.5020504024130901 1105 34-35 0.5367358224675627 1105 36-37 0.41040725064948447 1105 38-39 0.3177919185632945 1105 40-41 0.41861415440843075 1105 42-43 0.22805712841755366 1105 44-45 0.408854894075553 1105 46-47 0.3969468987108584 1105 48-49 0.42687406953049134 1105 50-51 0.5191771565510592 1105 52-53 0.5489670283791526 1105 54-55 0.5595953533279285 1105 56-57 0.7673305929491363 1105 58-59 0.7277669792396892 1105 60-61 0.7719477130865116 1105 62-63 0.7901540158249247 1105 64-65 0.9453983587169184 1105 66-67 1.0637911809273568 1105 68-69 1.085335472665708 1105 70-71 1.1631512031955964 1105 72-73 1.3642144894903616 1105 74-75 1.5328636562019256 1105 76-77 1.43199390064402 1105 78-79 1.4879148855829207 1105 80-81 1.6124943549127728 1105 82-83 1.8138722999629415 1105 84-85 2.0160886292362328 1105 86-87 1.9788245720058981 1105 88-89 1.9283418746440724 1105 90-91 2.047054908144066 1105 92-93 1.8685183086071397 1105 94-95 1.8686976781963338 1105 96-97 1.7806956739429047 1105 98-99 2.0436807762387907 1105 100-101 1.9867840900594231 1106 1 0.037679922657268605 1106 2 0.13371373490099359 1106 3 0.07858415874571989 1106 4 0.07723706222633098 1106 5 -0.02053484082259871 1106 6 0.04002048166862693 1106 7 0.10881107939972168 1106 8 0.04847318596925021 1106 9 0.11799848069351526 1106 10-11 0.18622518973413094 1106 12-13 0.1625130119421314 1106 14-15 0.18557569525659545 1106 16-17 0.0934288378123469 1106 18-19 0.17332751414998882 1106 20-21 0.23472247667703527 1106 22-23 0.319813450898657 1106 24-25 0.02108345806822598 1106 26-27 -0.061345799178468496 1106 28-29 0.09764710973333735 1106 30-31 0.09238807722832831 1106 32-33 -0.08364192198735054 1106 34-35 0.005962029885090203 1106 36-37 -0.025003080851334403 1106 38-39 0.14109709897240208 1106 40-41 0.13206033661724348 1106 42-43 0.24654315128513105 1106 44-45 0.4336995331610254 1106 46-47 -0.05578705749277191 1106 48-49 0.06893073453512244 1106 50-51 0.4203072413575697 1106 52-53 0.2841317730073243 1106 54-55 0.2104096606557917 1106 56-57 0.22815719968272674 1106 58-59 0.5432603785407792 1106 60-61 0.5505418252074463 1106 62-63 0.4925853017895889 1106 64-65 0.7374474536218933 1106 66-67 0.5089071559240956 1106 68-69 0.5192097159232354 1106 70-71 0.6042308171044652 1106 72-73 0.7627667279929042 1106 74-75 0.8370001673008147 1106 76-77 0.9234457066611519 1106 78-79 0.9577862901868244 1106 80-81 1.1772311454901114 1106 82-83 1.2566482314329441 1106 84-85 1.6414409197397468 1106 86-87 1.5979075895171704 1106 88-89 1.454958799781938 1106 90-91 1.7874412828206658 1106 92-93 1.524046006077068 1106 94-95 1.2150956749821198 1106 96-97 1.621104330926979 1106 98-99 1.8344660924924128 1106 100-101 2.22900815323953 1107 1 0.09413074425323487 1107 2 0.056508126712742524 1107 3 0.08050944517721348 1107 4 0.10757824715928166 1107 5 0.033374487553864185 1107 6 -0.08217502517650388 1107 7 -0.009980244722690657 1107 8 0.053461975192249156 1107 9 0.08457783177412637 1107 10-11 0.1282479807505439 1107 12-13 0.05031418464514559 1107 14-15 0.1191845816126289 1107 16-17 0.1629230955762253 1107 18-19 0.1116487470659493 1107 20-21 0.09428620227112816 1107 22-23 0.18016006002128648 1107 24-25 0.13400812724629674 1107 26-27 0.17629114329503182 1107 28-29 0.09786036176401325 1107 30-31 0.22557025019586519 1107 32-33 0.2110920088179924 1107 34-35 0.2758933103994181 1107 36-37 0.10789780876287836 1107 38-39 0.31452712574796493 1107 40-41 0.25390783708104436 1107 42-43 0.22694136478259708 1107 44-45 0.12042131043222781 1107 46-47 0.30102885681287717 1107 48-49 0.33085721413134905 1107 50-51 0.5008211342694651 1107 52-53 0.340966055770096 1107 54-55 0.5800946154502853 1107 56-57 0.46832870141399496 1107 58-59 0.340602787252557 1107 60-61 0.42656886922815573 1107 62-63 0.75618940763691 1107 64-65 0.5971753701966485 1107 66-67 0.7509105318476479 1107 68-69 0.8924999383307117 1107 70-71 0.9270410131157867 1107 72-73 1.1708696248451211 1107 74-75 0.9101289984782106 1107 76-77 0.9079764305222788 1107 78-79 0.8125524992381834 1107 80-81 1.1745791013712754 1107 82-83 1.3176695804469745 1107 84-85 1.3374759234252132 1107 86-87 1.614993787034603 1107 88-89 1.5205303976466986 1107 90-91 1.6873249492589046 1107 92-93 1.7592259711665328 1107 94-95 1.4223910400342668 1107 96-97 1.392533347175739 1107 98-99 1.444276104557506 1107 100-101 1.3640460113622996 1108 1 -0.06194394723372909 1108 2 0.056423525674020425 1108 3 0.01944596270397625 1108 4 -0.05601071357810383 1108 5 0.043440763358738366 1108 6 0.06114612211340642 1108 7 0.04930936110384465 1108 8 0.011955308056407432 1108 9 0.06973272030415245 1108 10-11 0.03283934650330167 1108 12-13 0.02393201205553197 1108 14-15 0.14484851883516114 1108 16-17 0.13261783057158283 1108 18-19 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0.4856279768615295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 27.0 5 74.0 6 147.0 7 362.0 8 902.0 9 1591.0 10 2668.0 11 4074.0 12 6064.0 13 8381.0 14 11410.0 15 14966.0 16 18885.0 17 23418.0 18 23765.0 19 22024.0 20 22432.0 21 24702.0 22 27405.0 23 30387.0 24 34078.0 25 39170.0 26 45220.0 27 53305.0 28 62055.0 29 72461.0 30 83675.0 31 97882.0 32 114521.0 33 137113.0 34 165187.0 35 199833.0 36 233864.0 37 213307.0 38 76227.0 39 1884.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.795560913572785 19.22009526972266 14.66744758937276 23.316896227331796 2 16.12671958660524 20.323925541966723 39.332717654155566 24.21663721727247 3 19.602284555232323 23.969895113293656 29.683791934665994 26.744028396808027 4 13.406530644924539 16.487810085536236 36.12036456317805 33.985294706361174 5 13.787905638100037 37.39951789942502 33.970049128140964 14.842527334333974 6 33.0169871495483 35.88219752065013 16.798836386928034 14.301978942873536 7 29.41854041286913 32.28615273755988 19.969521877793937 18.325784971777047 8 26.76304727845314 35.46046784718237 19.161718197467273 18.61476667689721 9 26.58568312118655 16.487880491089516 19.753857420493663 37.17257896723027 10-11 25.427534394640976 26.523572505637922 26.847382537797408 21.201510561923698 12-13 26.210491199508933 23.626496216923098 27.500420341878062 22.662592241689907 14-15 22.742417165961648 25.203405436421622 25.732235551581955 26.321941846034775 16-17 22.49720439290623 28.434753599594337 26.54481645071325 22.523225556786187 18-19 22.45412935854495 26.738360533233696 28.959527082026714 21.84798302619464 20-21 22.928061490011874 25.95938029597406 29.214347669437807 21.898210544576255 22-23 22.699715768825314 25.909900052042328 28.961341891405006 22.429042287727352 24-25 22.620247982188925 26.146459909866394 29.049460324611236 22.18383178333345 26-27 22.160643526434328 26.532840656316676 28.91635789832184 22.390157918927155 28-29 22.254690232232416 26.785699035158327 28.863103079422757 22.0965076531865 30-31 22.322208729766878 26.368352927047333 28.884719571918495 22.424718771267298 32-33 22.017820975181568 26.441625724292372 28.741800293733565 22.798753006792488 34-35 22.656845340809028 26.518380554223214 28.7093882063482 22.115385898619554 36-37 22.77722547008798 26.136211086216495 28.55434018209696 22.53222326159857 38-39 22.26134667788084 26.502909822074184 28.646913383846943 22.588830116198032 40-41 22.688721234550435 26.304556428526627 28.585399836912927 22.42132250001001 42-43 22.36777663397075 26.29155371312768 28.526532482711005 22.81413717019057 44-45 22.39774684634818 26.56449580269266 28.534270492665186 22.50348685829397 46-47 22.70933570468552 26.879769882048006 27.877301739976108 22.533592673290368 48-49 22.51631326812474 26.785705707308612 27.90269419127557 22.795286833291076 50-51 22.744739054430937 26.948478095517686 27.811179759537758 22.49560309051362 52-53 23.016266563471625 26.847195719184935 27.57514778686665 22.56138993047679 54-55 22.50553116534782 26.925152457331965 27.80517487556546 22.76414150175476 56-57 22.52291130730629 27.05259665930868 27.65241800729073 22.772074026094298 58-59 22.640254073312963 26.602116387995572 27.631833891565137 23.125795647126328 60-61 22.384072378868147 26.94482178838789 27.82342972284124 22.84767610990272 62-63 22.409826659015998 26.97297802212466 27.861487343039006 22.755707975820332 64-65 22.121331248437063 26.86348279626971 27.764242320885607 23.25094363440762 66-67 22.091949820319808 26.943328347035646 27.631303985791057 23.333417846853493 68-69 22.578631458988873 26.64147257340048 27.704748712939463 23.075147254671183 70-71 22.34532682457114 26.866632025148995 27.46474531678974 23.323295833490125 72-73 22.488812880259985 26.880686468942816 27.28661738382463 23.343883266972572 74-75 22.26795126703052 27.17519582593843 27.216749623026725 23.340103284004325 76-77 22.277532393012983 26.97391211518702 27.523265589346003 23.225289902453998 78-79 22.42386474332457 27.031719131560333 27.41395001267698 23.130466112438118 80-81 22.326754816725312 27.193814479212925 27.225413563693508 23.25401714036826 82-83 22.40248033646916 27.115189018367396 27.1548216650996 23.327508980063843 84-85 22.326781825169746 27.040985181705597 27.20048469060939 23.431748302515267 86-87 22.380132503003246 27.144246143958696 26.965773037830054 23.509848315208007 88-89 22.240595684490053 27.300310919547897 26.777859325584807 23.68123407037724 90-91 22.171928794470094 27.148976487229444 27.020123301929594 23.658971416370868 92-93 22.224760603375575 26.91992356388714 27.116431629178102 23.73888420355919 94-95 22.03383769074868 26.74113798277317 27.17338333672276 24.05164098975539 96-97 22.08943273862742 26.725576802466005 27.0585409466366 24.12644951226998 98-99 21.879315176343425 26.852079691482405 26.808177317551607 24.46042781462256 100-101 22.124332156286172 26.63325635510724 26.995978325820996 24.246433162785596 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 281.0 1 207.0 2 184.0 3 257.5 4 251.0 5 236.5 6 419.5 7 902.5 8 1393.5 9 1580.0 10 1421.0 11 1247.0 12 1303.0 13 1435.5 14 1582.5 15 1745.0 16 1910.0 17 2048.5 18 2246.0 19 2545.0 20 2884.5 21 3314.5 22 3910.5 23 4730.5 24 5742.5 25 7156.5 26 9174.5 27 11689.5 28 14046.5 29 16852.0 30 20010.0 31 23647.5 32 29208.5 33 32541.0 34 34108.0 35 37212.5 36 41262.5 37 45329.0 38 48045.0 39 50325.0 40 53423.0 41 57180.5 42 60497.5 43 64070.0 44 66938.0 45 69321.0 46 70578.0 47 71731.0 48 73006.5 49 71505.0 50 69953.0 51 67723.0 52 64798.5 53 64154.0 54 64153.5 55 60737.5 56 55458.0 57 51037.0 58 47658.0 59 42667.5 60 34997.0 61 28857.5 62 24406.0 63 18664.5 64 13529.5 65 10429.5 66 7713.0 67 5957.5 68 4608.5 69 3376.5 70 2570.0 71 1912.0 72 1524.5 73 1260.0 74 955.5 75 732.5 76 513.5 77 302.5 78 144.0 79 78.0 80 43.5 81 23.0 82 16.0 83 7.5 84 4.0 85 5.0 86 3.0 87 1.0 88 2.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025460708042541264 2 0.010942233016186497 3 0.0013344186605105486 4 0.0 5 3.736372249429536E-4 6 0.0 7 0.0 8 0.0 9 4.2701397136337554E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001814809378294346 26-27 0.009180800384312574 28-29 3.736372249429536E-4 30-31 0.0 32-33 4.537023445735865E-4 34-35 0.010915544642976288 36-37 0.0012009767944594937 38-39 0.013370874978315697 40-41 0.012943861006952322 42-43 0.005417739761672828 44-45 6.405209570450633E-4 46-47 5.337674642042194E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.668837321021097E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.668837321021097E-5 66-67 4.0032559815316457E-4 68-69 0.0010675349284084389 70-71 2.668837321021097E-5 72-73 1.868186124714768E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6013023926126583E-4 82-83 0.0010408465551982279 84-85 0.006138325838348524 86-87 0.004456958326105233 88-89 0.0 90-91 0.0013344186605105486 92-93 8.006511963063291E-4 94-95 1.0675349284084389E-4 96-97 0.0 98-99 0.0 100-101 8.006511963063291E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1873475.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.98740306656526 #Duplication Level Percentage of deduplicated Percentage of total 1 79.1860872365734 45.917955578513805 2 12.092106592811795 14.023797178424974 3 3.6845444535643272 6.409714930365363 4 1.6159125265809422 3.7481028399664376 5 0.85528344932282 2.4797833056022305 6 0.5326850917157128 1.853341507252159 7 0.36941170544335106 1.4994857822735614 8 0.24796719892678534 1.1503179129163736 9 0.19598953777068504 1.0228431891184675 >10 1.0958716028521807 11.606653059581715 >50 0.07985237601483314 3.180864940830534 >100 0.03995252653157026 4.298287086608765 >500 0.002767469292548488 1.1017909087199378 >1k 0.0014759836226925266 1.4100163403868933 >5k 9.224897641828291E-5 0.2970454394388413 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5553 0.29640107287260303 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4726 0.25225850358291413 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3185 0.17000493734904387 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0675349284084389E-4 0.0 10-11 0.0 0.0 0.0 1.0675349284084389E-4 0.0 12-13 0.0 0.0 0.0 1.0675349284084389E-4 5.337674642042194E-5 14-15 0.0 0.0 0.0 1.0675349284084389E-4 1.0675349284084389E-4 16-17 0.0 0.0 0.0 1.3344186605105486E-4 1.0675349284084389E-4 18-19 0.0 0.0 0.0 1.6013023926126583E-4 1.0675349284084389E-4 20-21 0.0 0.0 0.0 1.6013023926126583E-4 1.6013023926126583E-4 22-23 0.0 0.0 0.0 2.668837321021097E-4 2.1350698568168777E-4 24-25 0.0 0.0 0.0 3.736372249429536E-4 2.1350698568168777E-4 26-27 0.0 0.0 0.0 0.0012543535408799157 2.1350698568168777E-4 28-29 0.0 0.0 0.0 0.003709683876219325 2.1350698568168777E-4 30-31 0.0 0.0 0.0 0.013370874978315697 2.1350698568168777E-4 32-33 0.0 0.0 0.0 0.03221286646472464 2.1350698568168777E-4 34-35 0.0 0.0 0.0 0.061596765369166924 2.668837321021097E-4 36-37 0.0 0.0 0.0 0.11625455370367899 2.668837321021097E-4 38-39 0.0 0.0 0.0 0.20096345027288862 3.736372249429536E-4 40-41 0.0 0.0 0.0 0.2893019655986869 3.736372249429536E-4 42-43 0.0 0.0 0.0 0.37686651810138916 3.736372249429536E-4 44-45 0.0 0.0 0.0 0.4905589879768879 3.736372249429536E-4 46-47 0.0 0.0 0.0 0.6258957285258677 3.736372249429536E-4 48-49 0.0 0.0 0.0 0.7718811299857218 3.736372249429536E-4 50-51 0.0 0.0 0.0 0.9282216202511375 3.736372249429536E-4 52-53 0.0 0.0 0.0 1.0757549473571837 4.2701397136337554E-4 54-55 0.0 0.0 0.0 1.2411427961408612 4.2701397136337554E-4 56-57 0.0 0.0 0.0 1.4330855762686987 4.2701397136337554E-4 58-59 0.0 0.0 0.0 1.6433365804187405 4.2701397136337554E-4 60-61 0.0 0.0 0.0 1.8735771761032307 4.2701397136337554E-4 62-63 0.0 0.0 0.0 2.098186525040366 4.803907177837975E-4 64-65 0.0 0.0 0.0 2.3399831863248775 4.803907177837975E-4 66-67 0.0 0.0 0.0 2.614953495509681 4.803907177837975E-4 68-69 5.337674642042194E-5 0.0 0.0 2.9249122619730716 4.803907177837975E-4 70-71 5.337674642042194E-5 0.0 0.0 3.256541987483153 4.803907177837975E-4 72-73 5.337674642042194E-5 0.0 0.0 3.586623787347042 5.604558374144304E-4 74-75 5.337674642042194E-5 0.0 0.0 3.91019362414764 6.405209570450633E-4 76-77 5.337674642042194E-5 0.0 0.0 4.267897890284098 6.405209570450633E-4 78-79 5.337674642042194E-5 2.668837321021097E-5 0.0 4.659362948531472 6.938977034654853E-4 80-81 5.337674642042194E-5 5.337674642042194E-5 0.0 5.084855682621866 7.472744498859072E-4 82-83 5.337674642042194E-5 5.337674642042194E-5 0.0 5.529857617528924 8.540279427267511E-4 84-85 5.337674642042194E-5 5.337674642042194E-5 0.0 5.962502835639654 8.540279427267511E-4 86-87 5.337674642042194E-5 5.337674642042194E-5 0.0 6.410520556719465 9.07404689147173E-4 88-89 5.337674642042194E-5 5.337674642042194E-5 0.0 6.8972631073272925 9.60781435567595E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3885 0.0 54.67063 1 GTATCAA 6690 0.0 38.70865 1 ATCAACG 8560 0.0 30.019178 3 TCAACGC 8695 0.0 29.49847 4 CAACGCA 8810 0.0 29.113417 5 TATCAAC 9040 0.0 28.638464 2 AACGCAG 9140 0.0 28.166208 6 ACGCAGA 10435 0.0 24.670738 7 CGCAGAG 10490 0.0 24.496109 8 GTACATG 7345 0.0 24.453308 1 TACATGG 7525 0.0 23.609499 2 GAGTACT 6540 0.0 23.095356 12-13 ACATGGG 7745 0.0 22.139158 3 GCAGAGT 11435 0.0 21.848663 9 TAAGGTG 1070 0.0 21.75142 5 TATACCG 110 0.0028787393 21.59 5 GTACTTT 6970 0.0 21.50017 14-15 GGACCGA 785 0.0 20.57238 6 GCACCGT 325 2.1827873E-11 20.460678 6 AGTACTT 6850 0.0 20.281992 12-13 >>END_MODULE