FastQCFastQC Report
Thu 26 May 2016
SRR938443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094582
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26360.2408225240320049No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.1996195808080162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.1337496870951651No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.039.113681
GTATCAA39950.033.9192431
TACCTGG9650.027.5929832
ATCAACG50350.026.8198783
TCAACGC50950.026.5973634
TATCAAC51250.026.4416732
CAACGCA51700.026.3034925
AACGCAG53500.025.579886
GTACATG50900.025.1276871
TACATGG49700.025.0658722
ACATGGG52850.022.4022833
ACGCAGA61450.022.3488717
GTACCTG12450.022.150151
CGCAGAG62250.022.0616558
GAGTACT28850.020.91692412-13
AGTACTT30450.020.2859812-13
GTACTTT29750.020.12028514-15
GCAGAGT66700.020.0189029
GTATAGA2902.408342E-919.674391
GGCCGTT1707.9272715E-519.5672047
Produced by FastQC (version 0.11.5)