##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938443_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1094582 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.878170845126267 31.0 31.0 34.0 27.0 34.0 2 30.912221286299246 31.0 31.0 34.0 27.0 34.0 3 30.95047424496292 31.0 31.0 34.0 27.0 34.0 4 34.60522921078549 37.0 35.0 37.0 32.0 37.0 5 34.57478014438389 37.0 35.0 37.0 30.0 37.0 6 34.58348666431569 37.0 35.0 37.0 30.0 37.0 7 34.472417781399656 37.0 35.0 37.0 30.0 37.0 8 34.43141948250565 37.0 35.0 37.0 30.0 37.0 9 35.88368071099287 39.0 35.0 39.0 30.0 39.0 10-11 35.705184262120156 38.0 35.0 39.0 30.0 39.0 12-13 35.55041148127779 38.0 35.0 39.0 30.0 39.0 14-15 36.45869382102026 39.0 36.0 41.0 29.5 41.0 16-17 36.28106436977769 39.0 36.0 40.0 29.0 41.0 18-19 36.4173150115752 39.0 36.0 41.0 29.0 41.0 20-21 36.38623145639157 39.0 36.0 41.0 29.0 41.0 22-23 36.294014975579714 39.0 35.5 41.0 29.0 41.0 24-25 36.14564052761693 39.0 35.0 41.0 27.5 41.0 26-27 35.929509164228904 39.0 35.0 40.0 27.0 41.0 28-29 35.70706488869724 38.5 34.5 40.0 27.0 41.0 30-31 35.459331964165315 38.0 34.0 40.0 26.5 41.0 32-33 35.199146797590316 38.0 34.0 40.0 25.5 41.0 34-35 34.941990184380884 38.0 34.0 40.0 25.0 41.0 36-37 34.662818774655534 38.0 33.5 40.0 24.5 41.0 38-39 34.540171499257255 38.0 33.0 40.0 24.0 41.0 40-41 34.43198408159462 38.0 33.0 40.0 24.0 41.0 42-43 34.14272297552856 38.0 33.0 40.0 23.5 41.0 44-45 33.794676415289125 38.0 33.0 40.0 21.5 41.0 46-47 33.40354765563475 38.0 32.0 40.0 19.5 41.0 48-49 33.0442397189064 37.0 31.5 40.0 17.0 41.0 50-51 32.36625670804015 36.5 30.5 39.5 15.0 40.5 52-53 32.40163551017649 37.0 31.0 39.0 14.5 40.0 54-55 32.497681306654044 37.0 31.0 40.0 13.5 41.0 56-57 32.17006446296395 36.5 31.0 39.5 8.5 41.0 58-59 31.77894072805875 36.0 30.5 39.0 8.0 41.0 60-61 31.286586112324155 35.5 29.5 39.0 6.5 40.0 62-63 30.705759824298227 35.0 28.5 39.0 2.0 40.0 64-65 30.165997156905558 35.0 28.0 38.0 2.0 40.0 66-67 29.61535270998427 34.0 28.0 38.0 2.0 40.0 68-69 29.00384484670861 34.0 26.5 37.0 2.0 39.5 70-71 28.358949809150893 34.0 26.0 36.5 2.0 39.0 72-73 27.701031078530434 33.0 25.5 36.0 2.0 39.0 74-75 27.00685056030521 32.5 23.5 35.5 2.0 38.5 76-77 26.24484278016631 32.0 21.5 35.0 2.0 37.5 78-79 25.4460214036043 31.0 19.0 35.0 2.0 37.0 80-81 24.734138694040283 31.0 16.5 34.5 2.0 36.0 82-83 23.99851906937991 30.5 9.0 34.0 2.0 36.0 84-85 23.279314386679115 30.0 4.5 34.0 2.0 35.0 86-87 22.51632175570218 29.5 2.0 34.0 2.0 35.0 88-89 21.696047897736307 29.0 2.0 34.0 2.0 35.0 90-91 20.782583214414267 28.0 2.0 34.0 2.0 35.0 92-93 19.82292783912032 26.5 2.0 33.0 2.0 35.0 94-95 18.815265096630494 25.0 2.0 33.0 2.0 34.5 96-97 17.566946560422153 22.0 2.0 32.0 2.0 34.0 98-99 16.076966823865185 9.5 2.0 32.0 2.0 34.0 100-101 12.754053602197004 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4284356224242174 1101 2 0.5117208757698961 1101 3 0.5227337118516289 1101 4 0.4239507214493443 1101 5 0.6017498535570809 1101 6 0.5728028989725047 1101 7 0.48417033105690876 1101 8 0.5737003106825114 1101 9 0.7284046202458185 1101 10-11 0.7246585814100115 1101 12-13 0.7582918250793469 1101 14-15 0.9006422625819468 1101 16-17 0.7075102117560803 1101 18-19 1.0143895435408794 1101 20-21 0.9496995972463367 1101 22-23 1.088394671755033 1101 24-25 0.9152344465042646 1101 26-27 0.8937812479887342 1101 28-29 0.9200399084596995 1101 30-31 0.9839049011584393 1101 32-33 1.1117913364181433 1101 34-35 0.9355374929015383 1101 36-37 0.9364215919379575 1101 38-39 0.951134559058552 1101 40-41 1.0307398963121202 1101 42-43 1.0736987611326256 1101 44-45 1.0115958846176838 1101 46-47 1.0887140222455614 1101 48-49 1.1512924793619561 1101 50-51 1.0727865385122684 1101 52-53 1.1368412062352249 1101 54-55 1.0217928860920722 1101 56-57 1.1380300020571141 1101 58-59 1.1334446571278427 1101 60-61 1.2276155542030693 1101 62-63 1.3305379659696577 1101 64-65 1.3947488527847547 1101 66-67 1.6517476451815476 1101 68-69 1.314219299797955 1101 70-71 1.3246853617148098 1101 72-73 1.060623049935547 1101 74-75 1.114765618466265 1101 76-77 1.1953992604088342 1101 78-79 1.3326849197041426 1101 80-81 1.4501045786269238 1101 82-83 1.4211239688749764 1101 84-85 1.5599781133495902 1101 86-87 1.854813655976443 1101 88-89 1.7031302353491036 1101 90-91 1.4883311619171877 1101 92-93 1.514050479106416 1101 94-95 1.2986960490481927 1101 96-97 1.1866759464899452 1101 98-99 1.2405845205077846 1101 100-101 0.517323111724318 1102 1 0.5734085077363531 1102 2 0.5377181666446447 1102 3 0.559547818051211 1102 4 0.29710741481667213 1102 5 0.6284033339393602 1102 6 0.5653720215065192 1102 7 0.6739862699000483 1102 8 0.7501346706399588 1102 9 0.7437388316232045 1102 10-11 0.8426822323546901 1102 12-13 0.9612034316656164 1102 14-15 1.0208932081978972 1102 16-17 0.9257250950715274 1102 18-19 0.8913155769221746 1102 20-21 0.9069010427380775 1102 22-23 1.0097231742837067 1102 24-25 0.9683309579196191 1102 26-27 0.8098466419602275 1102 28-29 0.7935603182969189 1102 30-31 0.7694354538106154 1102 32-33 0.8939420443997506 1102 34-35 0.821128508729764 1102 36-37 1.2211750287305065 1102 38-39 0.9032772521540338 1102 40-41 1.176818460944439 1102 42-43 1.1790345192424283 1102 44-45 1.1664008322865769 1102 46-47 1.113992316902774 1102 48-49 1.4476384412186007 1102 50-51 1.289193619734469 1102 52-53 0.9517320209882456 1102 54-55 0.8578153465214982 1102 56-57 1.2485744987363248 1102 58-59 1.233849619795354 1102 60-61 1.0499259974590842 1102 62-63 1.176841041154919 1102 64-65 1.4109136125959587 1102 66-67 0.9250351077435894 1102 68-69 0.7554350314726896 1102 70-71 1.194065684466306 1102 72-73 1.2846194315191184 1102 74-75 1.4832352439226675 1102 76-77 1.3736325170304902 1102 78-79 1.6339157437375533 1102 80-81 1.7200792215895255 1102 82-83 1.6882020038686285 1102 84-85 1.648732900017695 1102 86-87 1.6281104926743133 1102 88-89 1.6939637366357019 1102 90-91 1.5150652598675372 1102 92-93 1.7809166586304883 1102 94-95 2.072102563135175 1102 96-97 1.7391167371045597 1102 98-99 2.1354273396853927 1102 100-101 2.205122673952175 1103 1 0.5338543416360402 1103 2 0.6131343147086916 1103 3 0.5312079061270367 1103 4 0.5256861282557423 1103 5 0.41046230405452633 1103 6 0.5162267052766367 1103 7 0.594135236559346 1103 8 0.475770564071226 1103 9 0.7274322772856436 1103 10-11 0.7124270221196269 1103 12-13 0.8117352714234087 1103 14-15 1.027665025710185 1103 16-17 0.9245777284179155 1103 18-19 0.9112761142611205 1103 20-21 0.8136649535037819 1103 22-23 1.0465259702514444 1103 24-25 0.9911512500072774 1103 26-27 0.8043514530822691 1103 28-29 1.1318335914501318 1103 30-31 1.250716453365527 1103 32-33 1.2457331890212515 1103 34-35 1.180281367462861 1103 36-37 1.2008538016034365 1103 38-39 1.3561379397211653 1103 40-41 1.2656605312327471 1103 42-43 1.4419849261733546 1103 44-45 1.3385800116018132 1103 46-47 1.1320219201173458 1103 48-49 1.3287418499147776 1103 50-51 1.419265819879989 1103 52-53 1.296173444877816 1103 54-55 1.3063384284469421 1103 56-57 1.3818884649825165 1103 58-59 1.403530622548498 1103 60-61 1.2853991274024636 1103 62-63 1.2926713250604251 1103 64-65 1.53707152898167 1103 66-67 1.507090701944481 1103 68-69 1.7243290908408078 1103 70-71 1.7555974966837375 1103 72-73 1.4475286167599748 1103 74-75 1.914398575097195 1103 76-77 1.8887828207295136 1103 78-79 1.5927544647635479 1103 80-81 1.4869763388252544 1103 82-83 1.5139537444633753 1103 84-85 1.6071238682032565 1103 86-87 1.817339815865683 1103 88-89 2.038343861029258 1103 90-91 1.745709055603399 1103 92-93 1.9541032818367015 1103 94-95 1.8930422932930178 1103 96-97 2.0057042474655056 1103 98-99 2.0025118201632814 1103 100-101 1.8819130856449195 1104 1 0.36013055849397446 1104 2 0.2729287957513371 1104 3 0.27001570995032154 1104 4 0.2582269746958872 1104 5 0.34487263328316686 1104 6 0.39656739187445567 1104 7 0.3354518924922587 1104 8 0.34741044809501176 1104 9 0.4972541487858635 1104 10-11 0.5749949486438908 1104 12-13 0.4760430998179217 1104 14-15 0.5879330849190936 1104 16-17 0.6980555405954192 1104 18-19 0.936933653361713 1104 20-21 0.929828685848058 1104 22-23 0.8471795650226923 1104 24-25 0.8306623083548956 1104 26-27 0.7571074400569273 1104 28-29 0.8140266902963305 1104 30-31 0.875366313309506 1104 32-33 0.6827040992690101 1104 34-35 0.7721389784666357 1104 36-37 0.9165374896677747 1104 38-39 1.037000312371056 1104 40-41 0.8843344089093961 1104 42-43 1.1721940920188274 1104 44-45 1.1464989317090968 1104 46-47 1.2697591941590147 1104 48-49 0.8541562631408226 1104 50-51 1.0903863456526253 1104 52-53 1.1271104364796258 1104 54-55 1.1850444815052867 1104 56-57 1.3121449627801596 1104 58-59 1.165396587771152 1104 60-61 1.3360818196852904 1104 62-63 1.5289987276742885 1104 64-65 1.3214253443767099 1104 66-67 1.289310699823595 1104 68-69 1.4810092328883968 1104 70-71 1.6280831483774705 1104 72-73 1.5817211672797207 1104 74-75 1.4822578517718519 1104 76-77 1.278860181313636 1104 78-79 1.5403479065724035 1104 80-81 1.5191142170605794 1104 82-83 1.6548762261060794 1104 84-85 1.8669219702695692 1104 86-87 1.985879069055418 1104 88-89 1.8192913277352396 1104 90-91 1.805675793914606 1104 92-93 1.6994509130406144 1104 94-95 2.0869338850174266 1104 96-97 2.2801364580228594 1104 98-99 2.3290893579189174 1104 100-101 1.724557622684113 1105 1 0.5941122249502264 1105 2 0.34166844507218386 1105 3 0.3171912896502427 1105 4 0.43128661688729863 1105 5 0.3969373230165303 1105 6 0.31393524356236924 1105 7 0.28974180805300165 1105 8 0.4605283635270254 1105 9 0.7603798620389384 1105 10-11 0.6432976724351605 1105 12-13 0.6502352826879658 1105 14-15 0.7314909707441402 1105 16-17 0.6410262381167087 1105 18-19 0.6312479278301595 1105 20-21 0.5296804034728666 1105 22-23 0.5938283585883752 1105 24-25 0.8452727019839585 1105 26-27 0.7050118065891766 1105 28-29 0.6463648731317164 1105 30-31 0.724785437527558 1105 32-33 0.7801013598820887 1105 34-35 0.9442913503657664 1105 36-37 0.8548678647800898 1105 38-39 0.8127919149841958 1105 40-41 0.6924659272070457 1105 42-43 0.8872629480165912 1105 44-45 1.0154386013290377 1105 46-47 0.9445133152455512 1105 48-49 0.9932594889632469 1105 50-51 0.9391890706816497 1105 52-53 1.0858240683085967 1105 54-55 0.9547339796969325 1105 56-57 0.7812371231231623 1105 58-59 0.9293602573514796 1105 60-61 1.0455040630317711 1105 62-63 1.1713758005950545 1105 64-65 1.3657763800016127 1105 66-67 1.207338271928684 1105 68-69 1.1264187917792725 1105 70-71 1.2136920839377616 1105 72-73 1.583205228781015 1105 74-75 1.471562459417676 1105 76-77 1.5266873122848494 1105 78-79 1.5583262162523432 1105 80-81 1.4558961677040827 1105 82-83 1.5054427439952711 1105 84-85 1.5914360917521932 1105 86-87 1.7124354770077126 1105 88-89 1.4834416499213994 1105 90-91 1.4747313168760918 1105 92-93 1.6916778469621256 1105 94-95 1.6061239513360377 1105 96-97 1.928260401294331 1105 98-99 1.9231855409473937 1105 100-101 1.5743493094642051 1106 1 0.2814904264814295 1106 2 0.25782713518810496 1106 3 0.1260242464974155 1106 4 0.4241701491747847 1106 5 0.4031889116922116 1106 6 0.016914040437299604 1106 7 0.688482218047227 1106 8 0.5212641341885984 1106 9 0.3960147967573562 1106 10-11 0.26662126988432533 1106 12-13 0.44336413048044676 1106 14-15 0.5317798499344519 1106 16-17 0.4938453281232711 1106 18-19 0.2941021725317725 1106 20-21 0.1933123998406998 1106 22-23 0.11342881964403517 1106 24-25 0.4575311233638999 1106 26-27 0.35676890187453836 1106 28-29 0.6301423961884893 1106 30-31 0.2584909406201774 1106 32-33 0.34313601419425055 1106 34-35 0.09580344987960387 1106 36-37 0.08789281518143355 1106 38-39 0.5854965883684642 1106 40-41 0.6416011281035807 1106 42-43 0.08851718248673279 1106 44-45 0.38735617084308416 1106 46-47 0.6755866924782978 1106 48-49 0.9548315225160735 1106 50-51 0.614756717491268 1106 52-53 0.6296203597200716 1106 54-55 0.6671029489241889 1106 56-57 -0.12713166065867654 1106 58-59 0.3807771562245783 1106 60-61 0.9259253438520716 1106 62-63 0.7092486298046232 1106 64-65 0.6077254912224994 1106 66-67 0.31987246342665543 1106 68-69 0.8027262219055196 1106 70-71 0.6725915453467337 1106 72-73 1.0476728485731535 1106 74-75 1.1566917356530055 1106 76-77 1.1986611548896526 1106 78-79 1.2323231723897088 1106 80-81 1.2190388119958016 1106 82-83 1.0032273907137643 1106 84-85 0.8864905343688285 1106 86-87 1.010043790717404 1106 88-89 1.021610632257726 1106 90-91 1.1037417737592357 1106 92-93 1.2771086318598321 1106 94-95 1.1606165263255264 1106 96-97 0.8303072550370523 1106 98-99 1.0596170116733958 1106 100-101 0.11983419326506883 1107 1 -0.04324029368644133 1107 2 0.12097863387095487 1107 3 0.15080629400539536 1107 4 0.14024013551265568 1107 5 -0.08179029318690567 1107 6 0.20389350195311096 1107 7 0.0912123796528661 1107 8 0.16468781873680172 1107 9 0.33908824769068246 1107 10-11 0.5227899858705314 1107 12-13 0.46593872495672173 1107 14-15 0.57814428914552 1107 16-17 0.6686085052856257 1107 18-19 0.5289787227306704 1107 20-21 0.7367877891359242 1107 22-23 0.5859981304235333 1107 24-25 0.7703154051207974 1107 26-27 0.5637860286002407 1107 28-29 0.8970181462847293 1107 30-31 0.8909781276554938 1107 32-33 1.0803536194719143 1107 34-35 0.9579835031694657 1107 36-37 0.8962644553163983 1107 38-39 0.8808658055510392 1107 40-41 0.7753450801578126 1107 42-43 0.9318642534316908 1107 44-45 0.9335368128877306 1107 46-47 1.0065872736849357 1107 48-49 0.89116901487359 1107 50-51 1.0845177434342261 1107 52-53 1.2055365150003823 1107 54-55 1.2865639363716284 1107 56-57 1.1738243110509003 1107 58-59 1.1191069796736208 1107 60-61 1.065313793690919 1107 62-63 1.0966413518897191 1107 64-65 1.2632945076246038 1107 66-67 1.1882050042054715 1107 68-69 1.1480452439687276 1107 70-71 1.3345583637309808 1107 72-73 1.1944822505341612 1107 74-75 1.1392257211454861 1107 76-77 1.4717456261048305 1107 78-79 1.653779887590904 1107 80-81 1.5818291322633051 1107 82-83 1.254781552379793 1107 84-85 1.6927211450886936 1107 86-87 1.6327542516822078 1107 88-89 1.621067921117426 1107 90-91 1.5545426312277435 1107 92-93 1.507486491660714 1107 94-95 1.8142268008513156 1107 96-97 1.9892450055408908 1107 98-99 1.9894257418500985 1107 100-101 1.7281563930944817 1108 1 0.45843286869904176 1108 2 0.42407898243085285 1108 3 0.3037024111754363 1108 4 0.3094141122029441 1108 5 0.30739499017244754 1108 6 0.455512564547881 1108 7 0.5174842863241764 1108 8 0.6511044657478351 1108 9 0.6475858459594903 1108 10-11 0.6353063187658279 1108 12-13 0.6398143291334506 1108 14-15 0.823075943700573 1108 16-17 0.8387955894753816 1108 18-19 0.7092706744040171 1108 20-21 0.7661270977817836 1108 22-23 0.7155617004855728 1108 24-25 0.9366308911390959 1108 26-27 0.9971010166758205 1108 28-29 1.0399707593607843 1108 30-31 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0.010236290902483347 2311 6 0.001666088469114868 2311 7 -0.1913069517609074 2311 8 -0.0017198884760318833 2311 9 0.038119413370409916 2311 10-11 -0.024329769295079018 2311 12-13 0.027462420652192066 2311 14-15 -0.09233433311775485 2311 16-17 -0.03732583128923039 2311 18-19 0.10140406816085346 2311 20-21 0.01185524535728888 2311 22-23 0.1912425120899215 2311 24-25 -0.019299053146887957 2311 26-27 0.19036468527070127 2311 28-29 0.22300825874194885 2311 30-31 0.22615315164999572 2311 32-33 -0.07633314824327186 2311 34-35 0.16964392221294133 2311 36-37 0.3544325435437088 2311 38-39 0.42982544047254123 2311 40-41 0.2481525266002862 2311 42-43 0.39103739408469806 2311 44-45 0.45494352410266714 2311 46-47 -0.1620460361066236 2311 48-49 -0.2507117913414447 2311 50-51 -0.14372332427552692 2311 52-53 -0.3762838069785843 2311 54-55 -0.12956760778729404 2311 56-57 -0.2998016645743178 2311 58-59 -0.36460392740882597 2311 60-61 -0.7491788811972526 2311 62-63 -0.7730223630013562 2311 64-65 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-1.7150337068858441 2312 86-87 -1.240484517039473 2312 88-89 -1.6644600934757072 2312 90-91 -1.3902146056303053 2312 92-93 -1.3831061986194157 2312 94-95 -1.3973788245662142 2312 96-97 -1.6269801634604697 2312 98-99 -1.6437040629632822 2312 100-101 -1.5394406896633992 2313 1 -0.24653827898836767 2313 2 -0.13318419061838682 2313 3 -0.17659751184589112 2313 4 -0.20438989773964522 2313 5 -0.17594808791683647 2313 6 -0.25626032485550354 2313 7 -0.42836106880606906 2313 8 -0.2483546268439909 2313 9 -0.341397682954522 2313 10-11 -0.4241233485805225 2313 12-13 -0.5098695294373385 2313 14-15 -0.4310455004428846 2313 16-17 -0.34838195214305046 2313 18-19 -0.3106585757296685 2313 20-21 -0.4046829264870624 2313 22-23 -0.4064076314453047 2313 24-25 -0.227109288203593 2313 26-27 -0.02978496086520721 2313 28-29 -0.11756815600381287 2313 30-31 -0.1057319206860825 2313 32-33 -0.20837063793999988 2313 34-35 -0.08158358823244072 2313 36-37 -0.15171211438768495 2313 38-39 -0.4489302796195531 2313 40-41 -0.2854734433283781 2313 42-43 -0.5230837723877428 2313 44-45 -0.2312849100843053 2313 46-47 -0.37648269585707084 2313 48-49 -0.526889917140835 2313 50-51 -0.4646453604861982 2313 52-53 -0.5373701270763576 2313 54-55 -0.5002636825961488 2313 56-57 -0.379763933283634 2313 58-59 -0.42609029866767045 2313 60-61 -0.6847759735429904 2313 62-63 -0.8492552027250859 2313 64-65 -0.735565064286984 2313 66-67 -0.8645798757788796 2313 68-69 -0.7278377582387776 2313 70-71 -0.7452600759258807 2313 72-73 -0.8430837650580933 2313 74-75 -0.8787961862510549 2313 76-77 -0.7988435543238204 2313 78-79 -0.7558667718105561 2313 80-81 -0.8667192232847967 2313 82-83 -0.6191995720508707 2313 84-85 -1.1668479721885276 2313 86-87 -1.0612585881758427 2313 88-89 -0.8765666948045556 2313 90-91 -0.8090422599338503 2313 92-93 -1.0546250991404165 2313 94-95 -1.0231146220117395 2313 96-97 -0.9496088380335941 2313 98-99 -0.9217682671475824 2313 100-101 -1.0049858792390456 2314 1 -0.5733761220948317 2314 2 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-1.0945966430507141 2314 62-63 -1.1598176563593476 2314 64-65 -1.3246627531749091 2314 66-67 -0.9836474303212341 2314 68-69 -0.91088089394351 2314 70-71 -0.9111306281993556 2314 72-73 -0.9491570675982608 2314 74-75 -1.0712431059415337 2314 76-77 -1.3136582538196855 2314 78-79 -1.1665095024750265 2314 80-81 -1.261292333593289 2314 82-83 -1.4527881989752167 2314 84-85 -1.4736253570796585 2314 86-87 -1.5676249086449978 2314 88-89 -1.6539123273550622 2314 90-91 -1.7867599690737954 2314 92-93 -1.66948995485836 2314 94-95 -1.872584698850254 2314 96-97 -2.0004327944322 2314 98-99 -2.2012298018602916 2314 100-101 -1.8268104691899332 2315 1 -0.6344660409102829 2315 2 -0.5664249287934524 2315 3 -0.5086820805510364 2315 4 -0.5957660788474612 2315 5 -0.5605396532137377 2315 6 -0.487489369716819 2315 7 -0.5329989085910469 2315 8 -0.7006406419732585 2315 9 -0.8267574878812312 2315 10-11 -0.8316474556491613 2315 12-13 -0.8791136204345946 2315 14-15 -1.1312249179939329 2315 16-17 -1.0299632511538306 2315 18-19 -1.1109273556903148 2315 20-21 -0.9732346319929022 2315 22-23 -0.935805093275782 2315 24-25 -1.079307672752897 2315 26-27 -1.2970776262203074 2315 28-29 -1.1997510926327024 2315 30-31 -1.133280827555211 2315 32-33 -0.9930605711606475 2315 34-35 -1.5085790023475454 2315 36-37 -1.5080308680245196 2315 38-39 -1.534631254986003 2315 40-41 -1.4934083403884202 2315 42-43 -1.4304523611544013 2315 44-45 -1.645726291114535 2315 46-47 -1.7394554853497155 2315 48-49 -1.7396715126408466 2315 50-51 -1.4567371336026582 2315 52-53 -1.250814666963855 2315 54-55 -1.587162737709896 2315 56-57 -1.623132064847269 2315 58-59 -1.7518038124807447 2315 60-61 -1.662559023779906 2315 62-63 -1.7670999753704884 2315 64-65 -1.635840425200282 2315 66-67 -1.6791095801209224 2315 68-69 -1.7869617295319706 2315 70-71 -2.2589183235913737 2315 72-73 -2.293685749042133 2315 74-75 -2.3820478695894707 2315 76-77 -2.2789808537779486 2315 78-79 -2.4815678164501946 2315 80-81 -2.658500652529167 2315 82-83 -2.4211395676173844 2315 84-85 -2.43173200887561 2315 86-87 -2.5086383299254074 2315 88-89 -2.445129137654174 2315 90-91 -2.31753688433464 2315 92-93 -2.2350024301831155 2315 94-95 -2.8877045940253705 2315 96-97 -2.9329254303470407 2315 98-99 -3.102177810221649 2315 100-101 -2.5346545455916747 2316 1 -0.9073433495616499 2316 2 -0.7654979462765112 2316 3 -0.8587278661616757 2316 4 -0.6621657801844023 2316 5 -0.7287024527572896 2316 6 -0.5262923225365057 2316 7 -0.7175247809830267 2316 8 -0.8048578489101317 2316 9 -0.8794771531531964 2316 10-11 -0.9862721545207194 2316 12-13 -1.0476145291028658 2316 14-15 -1.4245567720998409 2316 16-17 -1.3591170626298137 2316 18-19 -1.1388717226514231 2316 20-21 -1.2400161148831685 2316 22-23 -1.4310190881759652 2316 24-25 -1.381864623008596 2316 26-27 -1.176592439626333 2316 28-29 -1.215643278085551 2316 30-31 -1.256367693424501 2316 32-33 -1.4371707806474276 2316 34-35 -1.2854614953388293 2316 36-37 -1.2103768522742726 2316 38-39 -1.7444336020268523 2316 40-41 -1.6385001229279013 2316 42-43 -1.6246513242864253 2316 44-45 -1.3570068089850125 2316 46-47 -1.5600847205187023 2316 48-49 -1.5989878278149021 2316 50-51 -1.5554568974897904 2316 52-53 -1.7163926926883235 2316 54-55 -2.1968375894349563 2316 56-57 -2.1536242249419395 2316 58-59 -2.005574433615383 2316 60-61 -1.8315653351502306 2316 62-63 -2.240312575570538 2316 64-65 -2.3896430962623683 2316 66-67 -2.389136981047905 2316 68-69 -2.2975776516541693 2316 70-71 -2.822016882401229 2316 72-73 -2.644034776274008 2316 74-75 -2.6220761157892625 2316 76-77 -2.753115857933871 2316 78-79 -3.2451023951521343 2316 80-81 -3.230004392861357 2316 82-83 -2.920570065060435 2316 84-85 -2.786585635800364 2316 86-87 -3.062480799249048 2316 88-89 -3.7677634303721526 2316 90-91 -3.458454427971848 2316 92-93 -3.5467915205286893 2316 94-95 -3.9226354929730043 2316 96-97 -3.9246001975448195 2316 98-99 -3.7625017610267726 2316 100-101 -3.292902295052814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5267.0 3 1957.0 4 2072.0 5 2222.0 6 2580.0 7 3147.0 8 3875.0 9 4882.0 10 5849.0 11 7115.0 12 8272.0 13 9444.0 14 10594.0 15 11554.0 16 13314.0 17 15005.0 18 14945.0 19 14533.0 20 15875.0 21 17341.0 22 18898.0 23 20607.0 24 22928.0 25 25429.0 26 28758.0 27 31873.0 28 35976.0 29 41183.0 30 46465.0 31 53641.0 32 62128.0 33 72886.0 34 86796.0 35 103175.0 36 117279.0 37 109547.0 38 45341.0 39 1829.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.506918202217626 18.277046040946402 14.106572474837916 23.109463281998053 2 15.961087515593162 19.72128023061418 40.11064285897197 24.206989394820692 3 20.056413464598904 24.03197128840012 28.68864304032721 27.222972206673763 4 13.177424606615373 15.742427734667968 36.14144651395917 34.938701144757495 5 14.235781649024021 37.35754656643191 33.577332256078094 14.829339528465981 6 33.62650426131168 34.73856029847471 17.282727971030397 14.352207469183217 7 30.175456231894653 31.809940692138284 19.821710881027883 18.192892194939184 8 27.348126378737785 34.63334484756437 19.176494885305466 18.842033888392375 9 27.27193808083485 16.49283123006771 19.38382389224457 36.85140679685287 10-11 25.89862627293011 26.21287696600919 26.774469827637425 21.114026933423276 12-13 26.734244394078793 23.38817339496042 27.12917144843302 22.748410762527772 14-15 23.42273433837499 24.82712424482515 25.347190808958924 26.402950607840936 16-17 23.204646842102136 28.08210383655998 25.96133328032892 22.751916041008965 18-19 23.144145111577714 26.66468651539834 28.005172764390178 22.185995608633764 20-21 23.827246600436577 25.887506353042696 28.048043628492348 22.237203418028383 22-23 23.56810892639527 25.765288987323203 27.884652730402326 22.7819493558792 24-25 23.35878441711808 26.00091676915195 28.04669215888167 22.593606654848298 26-27 22.921963747286807 26.261346969426196 27.970916656421007 22.845772626865983 28-29 22.92679560409828 26.4735347375137 27.96075753711773 22.638912121270288 30-31 23.00746573273015 26.157124579872555 27.903090373624888 22.93231931377241 32-33 22.836210312337045 26.255497306588264 27.79019586246988 23.11809651860481 34-35 23.42540406161857 26.309152331035946 27.639089936362094 22.62635367098339 36-37 23.445293811704936 25.928527618857842 27.63805315639519 22.988125413042027 38-39 23.03180539784735 26.247529661200886 27.734553671180485 22.98611126977128 40-41 23.36034260897539 26.041864681883986 27.881806849254975 22.715985859885652 42-43 23.095366039556726 26.05193077115696 27.641090597372024 23.211612591914285 44-45 23.142340926585366 26.238542168702217 27.65882215892609 22.960294745786328 46-47 23.380948051728527 26.477267691509255 27.124719435293432 23.017064821468793 48-49 23.218553901593094 26.540143411093524 27.117680058767853 23.123622628545533 50-51 23.318378070446734 26.639250461289382 27.075208328289115 22.967163139974772 52-53 23.492982969081208 26.409893282654778 27.076602587530154 23.020521160733864 54-55 23.17082196174028 26.581249137708124 27.179485539536596 23.068443361014996 56-57 23.130677592253164 26.797230349203332 26.982988467098064 23.089103591445443 58-59 23.28283075185507 26.281780372480334 27.133871513793594 23.301517361871 60-61 23.050654472748274 26.51537581466414 27.35941903435571 23.07455067823188 62-63 23.0459946814124 26.48864032468876 27.450481445932184 23.01488354796665 64-65 22.854292192238972 26.54887268537659 27.269533564523474 23.32730155786096 66-67 22.899159183990463 26.50866056479263 27.168887295021115 23.423292956195795 68-69 23.094483642071754 26.255237771528083 27.38571013491659 23.264568451483573 70-71 23.024393364420003 26.32454575155186 27.138206218400235 23.512854665627906 72-73 23.006894497150405 26.32282366874612 27.090589826995597 23.57969200710788 74-75 22.86471998252718 26.635723691845985 26.999024921614485 23.50053140401235 76-77 22.996592890898036 26.346086804751216 27.04615017688914 23.61117012746161 78-79 23.135817448251668 26.349668532920127 27.06527379838615 23.449240220442054 80-81 23.077966727301465 26.537393641660216 26.893939047866162 23.490700583172153 82-83 23.117011027243535 26.48909358607202 26.943175365560958 23.450720021123484 84-85 23.009433716617565 26.39142866148667 26.894520623664096 23.704616998231664 86-87 22.99541953764102 26.296312878564294 26.96364594935633 23.74462163443835 88-89 23.072718980854 26.30324693306622 26.795217566541247 23.828816519538538 90-91 23.010865010571646 26.249622441225352 26.89772121288557 23.84179133531743 92-93 23.054779667274534 26.005480388443207 26.967724012134163 23.972015932148093 94-95 23.007152989521867 25.88379545338218 27.027670534728465 24.081381022367488 96-97 23.117035880693816 25.686164116381477 26.962936627042954 24.233863375881754 98-99 22.91797457203509 25.757123001132225 26.702927057946262 24.621975368886428 100-101 23.59661966371258 25.21904211615232 26.92287825020468 24.26145996993042 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 221.0 1 154.5 2 135.0 3 185.0 4 167.5 5 147.5 6 238.5 7 479.5 8 713.0 9 829.0 10 783.0 11 688.0 12 675.0 13 709.5 14 772.5 15 833.5 16 905.0 17 972.0 18 1027.0 19 1135.5 20 1273.0 21 1434.0 22 1685.5 23 1996.0 24 2337.0 25 2809.0 26 3502.0 27 4398.5 28 5183.0 29 6170.0 30 7691.0 31 9755.0 32 12360.5 33 14511.5 34 16415.5 35 18827.5 36 21879.0 37 25045.0 38 27320.5 39 29334.5 40 31411.0 41 33646.5 42 35766.0 43 37735.0 44 39953.5 45 41735.0 46 42745.5 47 43929.0 48 45391.5 49 45403.0 50 44158.0 51 42842.5 52 41536.5 53 40753.5 54 40312.5 55 38282.5 56 35153.0 57 32217.5 58 29662.0 59 26266.0 60 21752.0 61 17933.5 62 14639.0 63 11417.5 64 8730.5 65 6713.5 66 4898.5 67 3594.0 68 2718.5 69 1927.0 70 1463.0 71 1131.5 72 859.5 73 695.0 74 518.0 75 363.0 76 271.0 77 171.5 78 107.0 79 76.0 80 41.0 81 20.0 82 10.5 83 6.5 84 6.5 85 6.0 86 2.5 87 3.0 88 3.0 89 1.0 90 1.5 91 2.0 92 2.5 93 2.5 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007126007919004698 2 0.10542837357091567 3 0.11200622703461231 4 0.11419884485584451 5 0.1535746065621397 6 0.09336897555413848 7 0.026676850158325275 8 0.025945977551247876 9 0.023022487122938255 10-11 0.05033885081245626 12-13 0.05321666170282354 14-15 0.02731636368951801 16-17 0.030468251807539315 18-19 0.03558436005708115 20-21 0.05645990889672953 22-23 0.030925047186962693 24-25 0.04773511714974301 26-27 0.056871024738210575 28-29 0.05143515972307237 30-31 0.06555013694725474 32-33 0.04782647622562768 34-35 0.060159951470058884 36-37 0.05161787787484171 38-39 0.03192999702169413 40-41 0.03563003959502349 42-43 0.03252383101494452 44-45 0.0072630465328317115 46-47 0.012561872934142897 48-49 0.009684062043775615 50-51 0.00872479174698652 52-53 0.009318625740236912 54-55 0.010506293726737696 56-57 0.01352114323093199 58-59 0.019687880853147596 60-61 0.02430151418532371 62-63 0.010826050492334061 64-65 0.0091815871264099 66-67 0.010003818809371979 68-69 0.011693961713238478 70-71 0.030102815504000614 72-73 0.02772747953099905 74-75 0.028412672600134112 76-77 0.02306816666088059 78-79 0.004933390097772483 80-81 0.004659312870118456 82-83 0.005984019470446252 84-85 0.004613633332176118 86-87 0.013795220458586019 88-89 0.037548580188601674 90-91 0.034853487450003745 92-93 0.028915147517499833 94-95 0.045131383487029754 96-97 0.04165973860341208 98-99 0.025123745868285793 100-101 0.0717625541074127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1094582.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.49491150632386 #Duplication Level Percentage of deduplicated Percentage of total 1 84.42222047340071 56.98070300019543 2 9.358140081194685 12.632536732880354 3 2.6302378832377378 5.32583019509135 4 1.1077242988485174 2.9906301409674128 5 0.6207866726408856 2.094997076710091 6 0.3828045917045365 1.5502417224787275 7 0.2793404005079402 1.3197838928697139 8 0.1870745110631463 1.010126205543669 9 0.13942964938220218 0.8469712661768549 >10 0.8060618138418216 9.7295063249618 >50 0.04495011376162387 2.088388386262608 >100 0.01946038674980238 2.3315583761659036 >500 0.0013608643588051744 0.5214405083292906 >1k 4.082593076415523E-4 0.577286171366847 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2636 0.2408225240320049 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2185 0.1996195808080162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1464 0.1337496870951651 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.135907588467561E-5 0.0 0.0 0.0 0.0 8 9.135907588467561E-5 0.0 0.0 0.0 0.0 9 9.135907588467561E-5 0.0 0.0 1.8271815176935122E-4 0.0 10-11 9.135907588467561E-5 0.0 0.0 2.2839768971168902E-4 0.0 12-13 9.135907588467561E-5 0.0 0.0 2.740772276540268E-4 9.135907588467561E-5 14-15 1.8271815176935122E-4 0.0 0.0 2.740772276540268E-4 1.370386138270134E-4 16-17 1.8271815176935122E-4 0.0 0.0 2.740772276540268E-4 1.8271815176935122E-4 18-19 1.8271815176935122E-4 0.0 0.0 2.740772276540268E-4 1.8271815176935122E-4 20-21 1.8271815176935122E-4 0.0 0.0 3.6543630353870244E-4 1.8271815176935122E-4 22-23 1.8271815176935122E-4 0.0 0.0 9.592702967890939E-4 1.8271815176935122E-4 24-25 2.740772276540268E-4 0.0 0.0 0.0013247066003277963 1.8271815176935122E-4 26-27 3.1975676559636466E-4 0.0 0.0 0.002832131352424944 1.8271815176935122E-4 28-29 3.6543630353870244E-4 0.0 0.0 0.005481544553080537 1.8271815176935122E-4 30-31 3.6543630353870244E-4 0.0 0.0 0.012881629699739262 1.8271815176935122E-4 32-33 4.111158414810402E-4 0.0 0.0 0.03138184256638607 1.8271815176935122E-4 34-35 4.5679537942337804E-4 0.0 0.0 0.059474758400923824 1.8271815176935122E-4 36-37 4.5679537942337804E-4 0.0 0.0 0.10716419601272449 1.8271815176935122E-4 38-39 4.5679537942337804E-4 0.0 0.0 0.17595758015388524 1.8271815176935122E-4 40-41 5.024749173657159E-4 0.0 0.0 0.2511917791449156 1.8271815176935122E-4 42-43 5.938339932503914E-4 0.0 0.0 0.32692845305331164 1.8271815176935122E-4 44-45 6.395135311927293E-4 0.0 0.0 0.42317523949781743 1.8271815176935122E-4 46-47 6.851930691350671E-4 0.0 0.0 0.5426729107549731 2.2839768971168902E-4 48-49 7.308726070774049E-4 0.0 0.0 0.6732250301941746 2.740772276540268E-4 50-51 7.308726070774049E-4 0.0 0.0 0.8128216981459588 3.6543630353870244E-4 52-53 7.308726070774049E-4 0.0 0.0 0.9508195822697614 3.6543630353870244E-4 54-55 7.308726070774049E-4 0.0 0.0 1.097222501374954 4.111158414810402E-4 56-57 7.308726070774049E-4 0.0 0.0 1.2691146026519713 4.5679537942337804E-4 58-59 7.308726070774049E-4 0.0 0.0 1.46174521415481 4.5679537942337804E-4 60-61 7.308726070774049E-4 0.0 0.0 1.6699982276339278 4.5679537942337804E-4 62-63 7.308726070774049E-4 0.0 0.0 1.8777030866577378 4.5679537942337804E-4 64-65 8.222316829620805E-4 0.0 0.0 2.08974750178607 4.5679537942337804E-4 66-67 8.222316829620805E-4 0.0 0.0 2.3443195667387187 4.5679537942337804E-4 68-69 8.222316829620805E-4 0.0 0.0 2.6314154627063115 4.5679537942337804E-4 70-71 8.222316829620805E-4 0.0 0.0 2.929657165931835 4.5679537942337804E-4 72-73 9.135907588467562E-4 0.0 0.0 3.2397755490223665 5.024749173657159E-4 74-75 0.0010049498347314318 0.0 0.0 3.5463309281533952 5.481544553080537E-4 76-77 0.0010049498347314318 0.0 0.0 3.882989122788425 5.938339932503914E-4 78-79 0.0010049498347314318 0.0 0.0 4.25979963127477 6.395135311927293E-4 80-81 0.0010049498347314318 0.0 0.0 4.659130151966687 6.851930691350671E-4 82-83 0.0010049498347314318 0.0 0.0 5.061429842624856 7.308726070774049E-4 84-85 0.0010049498347314318 0.0 0.0 5.474281506547705 7.308726070774049E-4 86-87 0.0010049498347314318 0.0 0.0 5.900197518322063 7.765521450197428E-4 88-89 0.0010049498347314318 0.0 0.0 6.3659004076441965 8.222316829620805E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2650 0.0 39.11368 1 GTATCAA 3995 0.0 33.919243 1 TACCTGG 965 0.0 27.592983 2 ATCAACG 5035 0.0 26.819878 3 TCAACGC 5095 0.0 26.597363 4 TATCAAC 5125 0.0 26.441673 2 CAACGCA 5170 0.0 26.303492 5 AACGCAG 5350 0.0 25.57988 6 GTACATG 5090 0.0 25.127687 1 TACATGG 4970 0.0 25.065872 2 ACATGGG 5285 0.0 22.402283 3 ACGCAGA 6145 0.0 22.348871 7 GTACCTG 1245 0.0 22.15015 1 CGCAGAG 6225 0.0 22.061655 8 GAGTACT 2885 0.0 20.916924 12-13 AGTACTT 3045 0.0 20.28598 12-13 GTACTTT 2975 0.0 20.120285 14-15 GCAGAGT 6670 0.0 20.018902 9 GTATAGA 290 2.408342E-9 19.67439 1 GGCCGTT 170 7.9272715E-5 19.567204 7 >>END_MODULE