FastQCFastQC Report
Thu 26 May 2016
SRR938443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094582
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.2340619524165389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22830.2085727702447144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.1441646217460181No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26550.036.5187841
GTATCAA39700.032.323911
TATCAAC51400.024.9649932
TCAACGC51050.024.9396954
ATCAACG51250.024.8423713
CAACGCA52150.024.4136415
TAAGGTG4800.023.7531975
AACGCAG53600.023.7423486
GTACATG51050.023.1821781
GTAAGGT4100.023.1738514
TACATGG52050.022.6444472
TGTACCG1503.0298881E-522.1696535
ACATGGG52100.021.5192133
ACGCAGA61650.020.4881277
CGCAGAG62000.020.2958778
AGTACTT30350.019.94826712-13
TAGACTC4050.019.9409565
GAGTACT28750.019.819712-13
GGACCGA2902.4483597E-919.648846
GTACTTT29950.019.10486214-15
Produced by FastQC (version 0.11.5)