##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938443_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1094582 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75838996073387 31.0 31.0 34.0 30.0 34.0 2 31.980335872506583 31.0 31.0 34.0 30.0 34.0 3 32.049859215664064 33.0 31.0 34.0 30.0 34.0 4 35.7278933876128 37.0 35.0 37.0 33.0 37.0 5 35.626287477776906 37.0 35.0 37.0 33.0 37.0 6 35.62455439610737 37.0 35.0 37.0 33.0 37.0 7 35.55723463386023 37.0 35.0 37.0 33.0 37.0 8 35.53286094600496 37.0 35.0 37.0 33.0 37.0 9 37.111723927490125 39.0 37.0 39.0 33.0 39.0 10-11 37.00392204512773 39.0 37.0 39.0 33.0 39.0 12-13 36.89760931570225 39.0 37.0 39.0 33.0 39.0 14-15 37.93068906669395 40.0 37.0 41.0 33.0 41.0 16-17 37.835677911750786 40.0 37.0 41.0 32.5 41.0 18-19 38.067314737497966 40.0 37.0 41.0 33.0 41.0 20-21 38.03000688847432 40.0 37.0 41.0 33.0 41.0 22-23 37.925474290642455 40.0 37.0 41.0 32.5 41.0 24-25 37.80368533376211 40.0 37.0 41.0 32.0 41.0 26-27 37.61664041615886 40.0 37.0 41.0 32.0 41.0 28-29 37.514850417785055 39.0 36.5 41.0 32.0 41.0 30-31 37.27189465933114 39.0 36.0 41.0 31.0 41.0 32-33 37.100263845011156 39.0 36.0 41.0 31.0 41.0 34-35 37.030743699421336 39.0 36.0 41.0 31.0 41.0 36-37 37.01045924380266 39.0 36.0 41.0 31.0 41.0 38-39 36.86394440982951 39.0 35.5 41.0 30.0 41.0 40-41 36.704246461206196 39.0 35.0 40.5 30.0 41.0 42-43 36.51437809136273 39.0 35.0 40.0 30.0 41.0 44-45 36.24206957541783 39.0 35.0 40.0 30.0 41.0 46-47 35.890794385436635 38.0 35.0 40.0 28.5 41.0 48-49 35.57248885876069 38.0 34.5 40.0 28.0 41.0 50-51 35.20874863646579 38.0 34.0 40.0 27.0 41.0 52-53 34.71896303794507 38.0 33.0 40.0 26.0 41.0 54-55 34.36702777864061 37.5 33.0 40.0 25.0 41.0 56-57 34.03764085285525 37.0 33.0 40.0 24.0 41.0 58-59 33.55625937572516 36.5 32.0 39.0 23.0 41.0 60-61 33.15035054477417 36.0 31.5 39.0 22.5 40.0 62-63 32.653095885004504 35.5 31.0 39.0 21.5 40.0 64-65 32.11291296586277 35.0 31.0 38.5 20.0 40.0 66-67 31.592311951046153 35.0 30.0 38.0 18.0 40.0 68-69 31.08126389799942 34.0 29.0 37.5 16.5 39.5 70-71 30.487336718491626 34.0 29.0 37.0 12.5 39.0 72-73 29.971285385654067 34.0 28.5 36.0 8.0 39.0 74-75 29.365672009954483 33.5 28.0 36.0 7.0 38.5 76-77 28.16166216875483 31.5 26.5 34.5 7.0 36.5 78-79 28.845643816543667 33.0 28.0 35.0 3.5 37.0 80-81 28.46116782479522 33.0 27.0 35.0 2.0 36.5 82-83 28.008819805185908 33.0 26.0 35.0 2.0 36.0 84-85 27.43615919136255 32.5 26.0 35.0 2.0 36.0 86-87 26.809109322097385 32.0 25.0 34.5 2.0 35.0 88-89 26.056138781744995 31.5 23.5 34.0 2.0 35.0 90-91 25.431065466086597 31.0 21.5 34.0 2.0 35.0 92-93 24.577530509363392 31.0 18.5 34.0 2.0 35.0 94-95 23.74490033638412 31.0 11.0 34.0 2.0 35.0 96-97 22.659471834910494 30.0 2.0 34.0 2.0 35.0 98-99 21.27507304158117 29.5 2.0 34.0 2.0 35.0 100-101 17.3525249821393 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14379843617104626 1101 2 0.24913090593534193 1101 3 0.3300099269356451 1101 4 0.21886197761784842 1101 5 0.12348034411688502 1101 6 0.25300186667887203 1101 7 0.2501875905246749 1101 8 0.22829918430124962 1101 9 0.2740878818628758 1101 10-11 0.2820380700505183 1101 12-13 0.3129975407830017 1101 14-15 0.29581523528273124 1101 16-17 0.3241085586147179 1101 18-19 0.40088566285467664 1101 20-21 0.23892967874565585 1101 22-23 0.24188425668533853 1101 24-25 0.4634106316642459 1101 26-27 0.18449807034551924 1101 28-29 0.17867776105240551 1101 30-31 0.21735605280337467 1101 32-33 0.3780529580493237 1101 34-35 0.348290740437335 1101 36-37 0.2207465817006522 1101 38-39 0.5168970347955408 1101 40-41 0.6165808676031759 1101 42-43 0.4943777559768989 1101 44-45 0.6077532478525356 1101 46-47 0.35076744700120344 1101 48-49 0.44834157928383434 1101 50-51 0.45955679079015965 1101 52-53 0.751775124640389 1101 54-55 0.843297313830341 1101 56-57 0.47819337010636076 1101 58-59 0.5776598252936438 1101 60-61 0.6683819616398594 1101 62-63 0.44938454604386635 1101 64-65 0.5117103503186371 1101 66-67 0.12084497626759472 1101 68-69 0.6295518304645569 1101 70-71 0.6331029046238825 1101 72-73 0.6660026601420981 1101 74-75 0.726847145179061 1101 76-77 0.8971622547603104 1101 78-79 0.8522479148308761 1101 80-81 0.8225525182901272 1101 82-83 0.8078375189297446 1101 84-85 1.0780295695556035 1101 86-87 0.9951240023352348 1101 88-89 0.9725509879809273 1101 90-91 1.0226696973716933 1101 92-93 1.2910377331669274 1101 94-95 1.2337853286794136 1101 96-97 1.1204283144298586 1101 98-99 1.1378414834539825 1101 100-101 1.4453197631432069 1102 1 0.27266655614123536 1102 2 0.2807573653711124 1102 3 0.2741672624312841 1102 4 0.2814437083645487 1102 5 0.18115752700158794 1102 6 0.19279647936301814 1102 7 0.16492045108908826 1102 8 0.2609343007514511 1102 9 0.3304854952078955 1102 10-11 0.44817306698049464 1102 12-13 0.4528966750815542 1102 14-15 0.5802911976549439 1102 16-17 0.4630295018402606 1102 18-19 0.4730899440225045 1102 20-21 0.46274373338749086 1102 22-23 0.6687477177100192 1102 24-25 0.6165793239254214 1102 26-27 0.521142504686722 1102 28-29 0.5080891121934741 1102 30-31 0.5836705644343354 1102 32-33 0.5756124017881064 1102 34-35 0.5010540013242206 1102 36-37 0.6457066244467029 1102 38-39 0.6189687188121766 1102 40-41 0.4601724740019648 1102 42-43 0.6391369109922991 1102 44-45 0.6139458019764561 1102 46-47 0.5758235746394362 1102 48-49 0.7036269514135967 1102 50-51 0.8226850336552758 1102 52-53 0.7311838978941054 1102 54-55 0.4637858937893711 1102 56-57 0.7405407474575085 1102 58-59 0.8126159777733335 1102 60-61 0.9335242375862904 1102 62-63 0.9711297289098226 1102 64-65 0.8750785710607971 1102 66-67 0.8526425136821096 1102 68-69 1.0595968731871785 1102 70-71 0.9221684438944777 1102 72-73 0.903307033645163 1102 74-75 1.0759671481787834 1102 76-77 1.0289513554525875 1102 78-79 1.1482971852887545 1102 80-81 0.9990459353032435 1102 82-83 1.1233709306933477 1102 84-85 0.7850025204866888 1102 86-87 0.7553336080122115 1102 88-89 0.8698896708211059 1102 90-91 0.9076870160876247 1102 92-93 1.3295096552866035 1102 94-95 1.4027231922201153 1102 96-97 1.2555935101383788 1102 98-99 1.5792728202526227 1102 100-101 1.7164141466297274 1103 1 0.23563011136986134 1103 2 0.21399777222208982 1103 3 0.2354286461474686 1103 4 0.1859568615484335 1103 5 0.06970527086919276 1103 6 0.19305144973048272 1103 7 0.1959682016196851 1103 8 0.21981211131113554 1103 9 0.41288179023081995 1103 10-11 0.3435081753340157 1103 12-13 0.49916580119061393 1103 14-15 0.5782905242488852 1103 16-17 0.43538387333830997 1103 18-19 0.560436839647231 1103 20-21 0.3465860746373508 1103 22-23 0.38249728350525913 1103 24-25 0.5095486270923004 1103 26-27 0.5307358758125318 1103 28-29 0.490687162514142 1103 30-31 0.4304955614804129 1103 32-33 0.4534642720001969 1103 34-35 0.4284607394714328 1103 36-37 0.488446765222335 1103 38-39 0.32583808247898816 1103 40-41 0.47586159138694484 1103 42-43 0.7023335819854708 1103 44-45 0.7661324616070075 1103 46-47 0.6461794048878104 1103 48-49 0.5666390131999322 1103 50-51 0.7745862508500494 1103 52-53 0.6389543641522835 1103 54-55 0.6986629083440121 1103 56-57 0.7210794900715953 1103 58-59 0.8131245859105292 1103 60-61 0.9668365158753431 1103 62-63 1.09332672061484 1103 64-65 0.7949969983111771 1103 66-67 0.9465186940305621 1103 68-69 1.055859824926717 1103 70-71 1.201295698983003 1103 72-73 0.9338412898468533 1103 74-75 0.8956355800932521 1103 76-77 0.910871646563006 1103 78-79 1.1962222861886147 1103 80-81 1.10846642408233 1103 82-83 1.0173259119881166 1103 84-85 1.2592620625528035 1103 86-87 1.0305243693786004 1103 88-89 0.8086274875462713 1103 90-91 1.2796097147579566 1103 92-93 1.468242408140366 1103 94-95 1.5717614386636356 1103 96-97 1.7598951356411021 1103 98-99 1.9239936454682152 1103 100-101 2.4433364410787455 1104 1 0.17195958314010085 1104 2 0.15628900080955432 1104 3 0.13253984519772644 1104 4 0.09268446487046589 1104 5 -0.0018383973829685374 1104 6 -0.0895332369604418 1104 7 0.13759212727489256 1104 8 0.06918269980507574 1104 9 0.09317643809919929 1104 10-11 0.10971160961631199 1104 12-13 0.18547910767664177 1104 14-15 0.23843676187598817 1104 16-17 0.18299852400004823 1104 18-19 0.10418656483739852 1104 20-21 0.19972640740942182 1104 22-23 0.18507390048630867 1104 24-25 0.19016399419943752 1104 26-27 0.06393031702391738 1104 28-29 0.0694741713158109 1104 30-31 0.21499033815377544 1104 32-33 0.19489861120217 1104 34-35 0.2583062510832761 1104 36-37 0.29577080287063495 1104 38-39 0.35358676274518075 1104 40-41 0.40789627044786414 1104 42-43 0.27532695292571674 1104 44-45 0.4228904556633566 1104 46-47 0.4079212207277152 1104 48-49 0.44015277696952637 1104 50-51 0.546302693659861 1104 52-53 0.4945776845792551 1104 54-55 0.7458590721280771 1104 56-57 0.6322777643951696 1104 58-59 0.6250550829477604 1104 60-61 0.38678732168243357 1104 62-63 0.6727887916851927 1104 64-65 0.7408885589982361 1104 66-67 0.9509492751272681 1104 68-69 0.7678112721722776 1104 70-71 0.8320599160271627 1104 72-73 0.8717497865788815 1104 74-75 0.6119632930805743 1104 76-77 0.8040747265022752 1104 78-79 0.8503511584207963 1104 80-81 0.8378298222578522 1104 82-83 0.97626221042254 1104 84-85 1.014264894710596 1104 86-87 1.0704461552674722 1104 88-89 0.813861159962606 1104 90-91 0.6569268465968428 1104 92-93 0.8340640830707251 1104 94-95 1.0170943271283477 1104 96-97 0.9685863683668821 1104 98-99 1.2775309976205236 1104 100-101 1.2909792839961547 1105 1 0.21612990735617288 1105 2 0.17825657672691264 1105 3 0.251769767046234 1105 4 0.12513825349752494 1105 5 0.11892138269372765 1105 6 0.09818383333629299 1105 7 0.18534697368257724 1105 8 0.2059366773126925 1105 9 0.36018868439152385 1105 10-11 0.15780064835539775 1105 12-13 0.1574403710721839 1105 14-15 0.32963413586691104 1105 16-17 0.14375444229869316 1105 18-19 0.2271247838150643 1105 20-21 0.2641945836352235 1105 22-23 0.2875570732835513 1105 24-25 0.15738220198785058 1105 26-27 -0.02485097018593052 1105 28-29 0.11160344681159273 1105 30-31 0.3334726593794457 1105 32-33 0.4328150829233124 1105 34-35 0.42657580319128385 1105 36-37 0.17685403161414115 1105 38-39 0.24782850510022314 1105 40-41 0.2516205097889781 1105 42-43 0.36292990273003056 1105 44-45 0.17638771652398333 1105 46-47 0.1961039323395397 1105 48-49 0.19538041145717955 1105 50-51 0.33363507145067217 1105 52-53 0.4379357976550864 1105 54-55 0.5274866630469646 1105 56-57 0.47247441801595613 1105 58-59 0.6583282616614383 1105 60-61 0.5765120214663213 1105 62-63 0.605149501559211 1105 64-65 0.46890427346430386 1105 66-67 0.22238399993844737 1105 68-69 0.4040936671678068 1105 70-71 0.4166402818563668 1105 72-73 0.5817587231293686 1105 74-75 0.5128220435973354 1105 76-77 0.2726730248444369 1105 78-79 0.6173002794949625 1105 80-81 0.7090939852050795 1105 82-83 0.6367289904670486 1105 84-85 0.5489602474567903 1105 86-87 0.4093815422745699 1105 88-89 0.40127473411245873 1105 90-91 0.6348713904738226 1105 92-93 0.9840953981299272 1105 94-95 0.8887248638907437 1105 96-97 1.0740599436554596 1105 98-99 0.8212445923170151 1105 100-101 0.9562844263497077 1106 1 -0.010187834174487875 1106 2 0.154260579789959 1106 3 0.10624387742781494 1106 4 0.0579262813974708 1106 5 0.09365844188118899 1106 6 0.03623547348906442 1106 7 0.04559547053560209 1106 8 0.0903258504979263 1106 9 -0.19753279897994247 1106 10-11 0.1279835039269912 1106 12-13 -0.03660986864798588 1106 14-15 -0.03999334266698895 1106 16-17 0.1361391903726954 1106 18-19 0.10082926376058765 1106 20-21 0.16293938996467006 1106 22-23 0.17102981040987686 1106 24-25 0.15869910421815803 1106 26-27 0.22882996138951484 1106 28-29 -0.04391051674838309 1106 30-31 -0.004092346431711746 1106 32-33 -0.137826030644419 1106 34-35 -0.09047438377285744 1106 36-37 -0.035397835229659336 1106 38-39 -0.0767457298175529 1106 40-41 0.16749823411797848 1106 42-43 0.11638662120950016 1106 44-45 -0.1953583959166849 1106 46-47 -0.6540277978501763 1106 48-49 -0.37729180897986225 1106 50-51 -0.1430763419151475 1106 52-53 0.02301687406978914 1106 54-55 -0.030329941031880026 1106 56-57 -0.1617169200846007 1106 58-59 -0.13532694735631878 1106 60-61 -0.10219181101891905 1106 62-63 -0.1759637624239332 1106 64-65 -0.022100025352202124 1106 66-67 0.07908585327350792 1106 68-69 -0.45997644398753224 1106 70-71 -0.2319534504084153 1106 72-73 0.12428407399688979 1106 74-75 0.19738699025779738 1106 76-77 0.08431603922764452 1106 78-79 0.4669671971524032 1106 80-81 0.007081982410610976 1106 82-83 0.28978540264186137 1106 84-85 0.45364929866370574 1106 86-87 0.8026664999338671 1106 88-89 0.908798861999454 1106 90-91 0.9443621989358881 1106 92-93 0.8114661019112539 1106 94-95 0.7729681586802073 1106 96-97 1.0107219422134612 1106 98-99 1.095870863857062 1106 100-101 1.494534141387252 1107 1 0.16341557154549236 1107 2 0.16611870498337922 1107 3 0.20794220853444756 1107 4 0.14322854659376105 1107 5 0.09791030815836876 1107 6 0.13366533241435974 1107 7 0.19799162702360462 1107 8 0.09512797605365364 1107 9 0.2168577265969276 1107 10-11 0.19547782395294888 1107 12-13 0.29041850480370357 1107 14-15 0.3001593561698428 1107 16-17 0.32891411243816293 1107 18-19 0.2959803397476932 1107 20-21 0.35948422967995697 1107 22-23 0.2696783162559129 1107 24-25 0.2644104030263037 1107 26-27 0.31893925843139925 1107 28-29 0.18127799800445388 1107 30-31 0.1446512659562913 1107 32-33 0.07920030480994456 1107 34-35 0.09417006117479332 1107 36-37 0.3360445385088582 1107 38-39 0.39819445118680363 1107 40-41 0.34083103908163537 1107 42-43 0.36920471862578097 1107 44-45 0.25225450536121485 1107 46-47 0.42734452916536725 1107 48-49 0.4918680077513429 1107 50-51 0.7400331891190319 1107 52-53 0.8113851930239235 1107 54-55 0.44493103584817817 1107 56-57 0.47644948617129756 1107 58-59 0.3087338000603239 1107 60-61 0.6572701576865043 1107 62-63 0.40496850813153884 1107 64-65 0.20073988618361938 1107 66-67 0.11072462956933649 1107 68-69 0.43954122997818246 1107 70-71 0.6102557386950878 1107 72-73 0.761623204300399 1107 74-75 0.25359986285277003 1107 76-77 0.6595804127699125 1107 78-79 0.9981251570415957 1107 80-81 0.7184287212374798 1107 82-83 0.989280432192821 1107 84-85 1.0150398166924326 1107 86-87 1.1564613264399064 1107 88-89 1.2053335117280959 1107 90-91 1.4279449982384413 1107 92-93 1.453268823769907 1107 94-95 1.4388985044604183 1107 96-97 1.047970872264866 1107 98-99 0.9983557109695198 1107 100-101 1.003651537581483 1108 1 0.11514133614962674 1108 2 0.14153980352736184 1108 3 0.15557061597888122 1108 4 0.1660822450351631 1108 5 0.08693535130716157 1108 6 0.22867729427120054 1108 7 0.178192975587244 1108 8 0.14650742711861398 1108 9 0.22732350894729336 1108 10-11 0.28307530653432167 1108 12-13 0.25423304547963 1108 14-15 0.21512496817138782 1108 16-17 0.21395037240170467 1108 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-1.0318433182107825 2316 78-79 -1.4782429544530977 2316 80-81 -1.4250727491627266 2316 82-83 -1.3397674303993483 2316 84-85 -1.4948299783039438 2316 86-87 -1.4587061713225289 2316 88-89 -1.5969797048256247 2316 90-91 -1.3038827731462952 2316 92-93 -1.7705601836034042 2316 94-95 -1.7074840979348416 2316 96-97 -2.026512356648741 2316 98-99 -2.0653040938295284 2316 100-101 -2.191997995726279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 14.0 5 37.0 6 75.0 7 206.0 8 447.0 9 787.0 10 1265.0 11 1917.0 12 2747.0 13 3913.0 14 5076.0 15 6579.0 16 8356.0 17 10281.0 18 10337.0 19 9577.0 20 9868.0 21 10693.0 22 11775.0 23 13235.0 24 15600.0 25 18349.0 26 21504.0 27 25614.0 28 30893.0 29 36424.0 30 42711.0 31 51086.0 32 60743.0 33 74490.0 34 93549.0 35 119726.0 36 153732.0 37 165697.0 38 74687.0 39 2584.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45125984395841 19.26729887262694 14.80513073507647 23.476310548338176 2 16.123851596727295 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23.550908017855217 25.854938232128795 28.01411863158722 22.58003511842877 22-23 23.266781291853878 25.818166204085212 27.935230069560802 22.97982243450011 24-25 23.141155806097206 25.982681866667274 28.042128152359375 22.834034174876145 26-27 22.724465473561693 26.333061384090694 27.937120340737483 23.005352801610137 28-29 22.725646948762563 26.507049961149498 27.97564911854359 22.79165397154435 30-31 22.91676088230605 26.10309954607476 27.899295015775692 23.080844555843495 32-33 22.678968515720214 26.233169312739708 27.819260025324827 23.268602146215255 34-35 23.23762952039365 26.248354798050748 27.703944510153537 22.81007117140206 36-37 23.260919526050284 25.851304284654937 27.745787283698647 23.141988905596136 38-39 22.83050810293255 26.19844647350216 27.791532896864624 23.179512526700666 40-41 23.118024401262772 26.005173699917183 27.904222142942302 22.97257975587774 42-43 22.99112526585831 25.967870429246968 27.65867447248595 23.382329832408768 44-45 22.897687248114888 26.212908760828036 27.65941797921785 23.22998601183923 46-47 23.13339704106225 26.400123517470597 27.19179558954925 23.274683851917903 48-49 23.045966405440616 26.414649610536262 27.19234374400456 23.34704024001856 50-51 23.156381529601042 26.496451127517307 27.171920008989808 23.175247333891846 52-53 23.340736463782523 26.389891300971513 27.071749763836788 23.197622471409176 54-55 22.985488749178327 26.42347709979631 27.143580848821607 23.44745330220375 56-57 22.993343577731043 26.602392511479266 26.996241487618107 23.408022423171584 58-59 23.05454897683831 26.234078706713753 27.134219752112827 23.577152564335115 60-61 22.88800656323601 26.42168425937938 27.321114361464012 23.3691948159206 62-63 22.85133970196765 26.403716473187917 27.38879749579366 23.356146329050777 64-65 22.69735414394703 26.440742701886265 27.240717482806936 23.62118567135976 66-67 22.69372719012489 26.57002977132679 27.012648131423255 23.723594907125065 68-69 22.889065037771754 26.30385073133513 27.225723625448587 23.581360605444523 70-71 22.834343536776384 26.294113320935903 27.032614748193716 23.838928394093998 72-73 22.895620742375733 26.312500542445257 26.873400357488553 23.918478357690457 74-75 22.71067476051535 26.59597799199327 26.80916799244103 23.884179255050356 76-77 22.70643008974695 26.351471476082942 27.04140370312988 23.900694731040232 78-79 22.85395473294466 26.388555704669773 26.96406956612332 23.793419996262244 80-81 22.74938555741217 26.581798426277626 26.813045419175722 23.855770597134473 82-83 22.74779879616953 26.549159457270967 26.89654479086507 23.80649695569443 84-85 22.69317169959846 26.430350275970994 26.808362690896498 24.068115333534053 86-87 22.64299373852756 26.39745593990156 26.86800289820379 24.09154742336709 88-89 22.668151030177235 26.395899864898535 26.66875452909939 24.26719457582484 90-91 22.513525578318134 26.433693025457465 26.86364215804309 24.189139238181312 92-93 22.560918216751425 26.15633809895869 26.843035927240827 24.43970775704906 94-95 22.333359834559243 26.12088762657762 26.946492197216642 24.599260341646495 96-97 22.45643490289392 25.946258174810595 26.949741737449145 24.647565184846343 98-99 22.28997271393509 26.027432321875015 26.74208041390721 24.94051455028269 100-101 22.35187911116959 25.89039744085261 26.986907304004742 24.770816143973057 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 139.0 2 130.0 3 186.0 4 173.5 5 146.0 6 246.0 7 507.0 8 749.5 9 864.5 10 786.0 11 659.0 12 627.0 13 649.5 14 695.0 15 740.0 16 820.5 17 891.5 18 940.0 19 1010.5 20 1106.5 21 1241.0 22 1486.0 23 1818.0 24 2178.0 25 2569.5 26 3269.5 27 4218.0 28 5010.5 29 6012.5 30 7555.0 31 9613.5 32 12303.5 33 14466.0 34 16409.5 35 19053.5 36 22015.0 37 24998.5 38 27550.5 39 29566.5 40 31421.0 41 33648.5 42 35939.0 43 37985.5 44 39970.0 45 42349.0 46 43439.5 47 44199.0 48 46120.0 49 46259.5 50 44785.0 51 43415.0 52 42045.5 53 41307.0 54 41001.0 55 38504.5 56 34936.5 57 32039.0 58 29596.0 59 26392.0 60 21689.5 61 17663.0 62 14432.5 63 10867.5 64 8041.0 65 6212.0 66 4502.0 67 3331.0 68 2408.0 69 1702.5 70 1308.0 71 932.5 72 708.0 73 573.0 74 441.0 75 354.5 76 240.0 77 133.5 78 85.0 79 52.0 80 27.0 81 14.0 82 7.0 83 5.0 84 3.5 85 1.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.8271815176935122E-4 2 0.05143515972307237 3 0.0 4 9.135907588467561E-5 5 0.04366963827287494 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005892660394561577 26-27 0.0013703861382701342 28-29 1.370386138270134E-4 30-31 0.0016901429038664987 32-33 3.6543630353870244E-4 34-35 0.010186536961141331 36-37 0.022520012205572537 38-39 0.0036543630353870244 40-41 6.851930691350671E-4 42-43 0.0012790270623854584 44-45 0.007765521450197427 46-47 0.0 48-49 0.0 50-51 8.222316829620805E-4 52-53 0.0 54-55 0.0015987838279818232 56-57 0.0 58-59 0.023159525736765267 60-61 0.0 62-63 0.009455664354063925 64-65 0.015759440590106543 66-67 0.0066235330016389815 68-69 0.016398954121299273 70-71 4.5679537942337804E-5 72-73 1.370386138270134E-4 74-75 0.023113846198822927 76-77 0.05129812110924536 78-79 0.0312904834905014 80-81 0.027179325075690996 82-83 0.03727450296094765 84-85 0.01585079966599122 86-87 0.010140857423198993 88-89 0.08765903331134625 90-91 0.02434719372326605 92-93 0.026220054778901897 94-95 0.026768209234209955 96-97 0.040517750154853635 98-99 0.056368549820844854 100-101 0.01416065676212472 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1094582.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.66910896067539 #Duplication Level Percentage of deduplicated Percentage of total 1 82.62224103575544 53.43106708116453 2 10.418700512147474 13.475361572974187 3 2.904569428513484 5.635077505691554 4 1.2637708488322772 3.2690773889783897 5 0.6926318348301659 2.239594179813226 6 0.43897165286055134 1.7032743389692055 7 0.29416654532410413 1.3316441860504962 8 0.20724854441494422 1.0722062960569119 9 0.16298751599673308 0.9486231688100297 >10 0.9189381951808495 10.853614993746854 >50 0.05133140057984312 2.2841225919130665 >100 0.02274705471401478 2.686687594354115 >500 0.0012715731374812165 0.4827902837794528 >1k 4.2385771249373884E-4 0.5868588176978474 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2562 0.2340619524165389 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2283 0.2085727702447144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1578 0.1441646217460181 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.135907588467561E-5 2 0.0 0.0 0.0 0.0 9.135907588467561E-5 3 0.0 0.0 0.0 0.0 9.135907588467561E-5 4 0.0 0.0 0.0 0.0 9.135907588467561E-5 5 0.0 0.0 0.0 0.0 9.135907588467561E-5 6 0.0 0.0 0.0 0.0 9.135907588467561E-5 7 0.0 0.0 0.0 0.0 9.135907588467561E-5 8 0.0 0.0 0.0 0.0 9.135907588467561E-5 9 0.0 0.0 0.0 9.135907588467561E-5 9.135907588467561E-5 10-11 0.0 0.0 0.0 9.135907588467561E-5 9.135907588467561E-5 12-13 0.0 0.0 0.0 9.135907588467561E-5 1.370386138270134E-4 14-15 9.135907588467561E-5 0.0 0.0 1.8271815176935122E-4 1.8271815176935122E-4 16-17 9.135907588467561E-5 0.0 0.0 1.8271815176935122E-4 1.8271815176935122E-4 18-19 1.370386138270134E-4 0.0 0.0 1.8271815176935122E-4 1.8271815176935122E-4 20-21 1.8271815176935122E-4 0.0 0.0 2.740772276540268E-4 1.8271815176935122E-4 22-23 1.8271815176935122E-4 0.0 0.0 8.679112209044183E-4 1.8271815176935122E-4 24-25 2.740772276540268E-4 0.0 0.0 0.0013247066003277963 1.8271815176935122E-4 26-27 2.740772276540268E-4 0.0 0.0 0.0030148495041942953 1.8271815176935122E-4 28-29 4.111158414810402E-4 0.0 0.0 0.005755621780734564 1.8271815176935122E-4 30-31 5.481544553080537E-4 0.0 0.0 0.013247066003277963 1.8271815176935122E-4 32-33 5.481544553080537E-4 0.0 0.0 0.03224975378729049 2.740772276540268E-4 34-35 5.938339932503914E-4 0.0 0.0 0.061073542228905646 2.740772276540268E-4 36-37 6.395135311927293E-4 0.0 0.0 0.10940249337189904 2.740772276540268E-4 38-39 8.222316829620805E-4 0.0 0.0 0.17842427520277146 2.740772276540268E-4 40-41 8.222316829620805E-4 0.0 0.0 0.25379551280762885 2.740772276540268E-4 42-43 8.222316829620805E-4 0.0 0.0 0.32976058440573663 2.740772276540268E-4 44-45 8.222316829620805E-4 0.0 0.0 0.42678392299526213 3.1975676559636466E-4 46-47 0.0010049498347314318 0.0 0.0 0.5470581463974376 3.6543630353870244E-4 48-49 0.0010963089106161073 0.0 0.0 0.6784324975196011 3.6543630353870244E-4 50-51 0.0010963089106161073 0.0 0.0 0.8185316403887511 3.6543630353870244E-4 52-53 0.0010963089106161073 0.0 0.0 0.9568949608160924 3.6543630353870244E-4 54-55 0.0010963089106161073 0.0 0.0 1.1046225865216128 3.6543630353870244E-4 56-57 0.0010963089106161073 0.0 0.0 1.2770171627159956 3.6543630353870244E-4 58-59 0.0010963089106161073 0.0 0.0 1.4698304923706036 3.6543630353870244E-4 60-61 0.0012333475244431207 0.0 0.0 1.680276123670954 3.6543630353870244E-4 62-63 0.0012790270623854586 0.0 0.0 1.889214330219207 3.6543630353870244E-4 64-65 0.0012790270623854586 0.0 0.0 2.101258745347539 3.6543630353870244E-4 66-67 0.0013703861382701342 0.0 0.0 2.3563332852175534 3.6543630353870244E-4 68-69 0.0013703861382701342 0.0 0.0 2.6444798105578204 3.6543630353870244E-4 70-71 0.0013703861382701342 0.0 0.0 2.9457820428254804 3.6543630353870244E-4 72-73 0.0013703861382701342 0.0 0.0 3.259463429875514 4.111158414810402E-4 74-75 0.0013703861382701342 0.0 0.0 3.5717744307872774 5.024749173657159E-4 76-77 0.0013703861382701342 0.0 0.0 3.912817861064772 5.481544553080537E-4 78-79 0.0013703861382701342 0.0 0.0 4.295703748097447 5.481544553080537E-4 80-81 0.0014617452141548097 0.0 0.0 4.7027997902395615 5.938339932503914E-4 82-83 0.0014617452141548097 0.0 0.0 5.1166107244592 6.395135311927293E-4 84-85 0.0015074247520971474 0.0 0.0 5.543120570226808 6.395135311927293E-4 86-87 0.0015531042900394853 0.0 0.0 5.981781173087079 6.851930691350671E-4 88-89 0.0015531042900394853 0.0 0.0 6.4632891825372605 7.308726070774049E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2655 0.0 36.518784 1 GTATCAA 3970 0.0 32.32391 1 TATCAAC 5140 0.0 24.964993 2 TCAACGC 5105 0.0 24.939695 4 ATCAACG 5125 0.0 24.842371 3 CAACGCA 5215 0.0 24.413641 5 TAAGGTG 480 0.0 23.753197 5 AACGCAG 5360 0.0 23.742348 6 GTACATG 5105 0.0 23.182178 1 GTAAGGT 410 0.0 23.173851 4 TACATGG 5205 0.0 22.644447 2 TGTACCG 150 3.0298881E-5 22.169653 5 ACATGGG 5210 0.0 21.519213 3 ACGCAGA 6165 0.0 20.488127 7 CGCAGAG 6200 0.0 20.295877 8 AGTACTT 3035 0.0 19.948267 12-13 TAGACTC 405 0.0 19.940956 5 GAGTACT 2875 0.0 19.8197 12-13 GGACCGA 290 2.4483597E-9 19.64884 6 GTACTTT 2995 0.0 19.104862 14-15 >>END_MODULE