FastQCFastQC Report
Thu 26 May 2016
SRR938442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100531
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26630.24197410159277655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21680.19699581383895592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.13466226757810548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25400.039.7436641
GTATCAA41100.035.9158061
AATACCG650.00583197629.2430555
ATCAACG49850.028.8890043
TATCAAC50550.028.7710292
TCAACGC50600.028.4608084
CAACGCA51550.028.0234135
AACGCAG53150.027.264266
TACCTGG10450.025.9246942
TACATGG50950.025.4667532
GTACATG51250.025.364981
ACATGGG51400.024.4115813
ACGCAGA60800.023.754587
GTACCTG12450.023.7130851
CGCAGAG61800.023.3702038
GGACCGA3500.023.076936
GAGTACT29050.022.7272712-13
GCAGAGT66000.021.4491569
GTACTTT31950.020.81352814-15
CATGGGA26250.020.2788734
Produced by FastQC (version 0.11.5)