##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938442_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1100531 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.830966142707474 31.0 31.0 34.0 27.0 34.0 2 30.91961516758728 31.0 31.0 34.0 27.0 34.0 3 30.977878860295622 31.0 31.0 34.0 27.0 34.0 4 34.60085540525437 37.0 35.0 37.0 31.0 37.0 5 34.62262217057039 37.0 35.0 37.0 30.0 37.0 6 34.644702420922265 37.0 35.0 37.0 30.0 37.0 7 34.550724150432835 37.0 35.0 37.0 30.0 37.0 8 34.50596848248709 37.0 35.0 37.0 30.0 37.0 9 35.971157559396325 39.0 35.0 39.0 30.0 39.0 10-11 35.80493098331624 38.0 35.0 39.0 30.0 39.0 12-13 35.64626348553561 38.0 35.0 39.0 30.0 39.0 14-15 36.58858814517719 39.0 36.0 41.0 30.0 41.0 16-17 36.425028463532605 39.0 36.0 40.5 29.5 41.0 18-19 36.40487864494503 39.0 36.0 40.5 29.5 41.0 20-21 36.485039494571254 39.0 36.0 41.0 29.0 41.0 22-23 36.39364543116005 39.0 36.0 41.0 29.0 41.0 24-25 36.26532510215523 39.0 35.5 41.0 28.5 41.0 26-27 36.08119671322298 39.0 35.0 40.5 27.0 41.0 28-29 35.888659201785316 39.0 35.0 40.0 27.0 41.0 30-31 35.670032920472025 38.0 34.5 40.0 27.0 41.0 32-33 35.398170065177624 38.0 34.0 40.0 26.5 41.0 34-35 35.153609030549795 38.0 34.0 40.0 25.0 41.0 36-37 34.86409106149668 38.0 34.0 40.0 25.0 41.0 38-39 34.61578955976705 38.0 33.0 40.0 24.0 41.0 40-41 34.5576517153992 38.0 33.0 40.0 24.0 41.0 42-43 34.384235428170584 38.0 33.0 40.0 24.0 41.0 44-45 34.0924362875739 38.0 33.0 40.0 23.0 41.0 46-47 33.73324240752873 38.0 33.0 40.0 21.5 41.0 48-49 33.370340771863766 38.0 32.0 40.0 18.5 41.0 50-51 32.653589494525825 36.5 31.0 39.5 17.5 40.5 52-53 32.72930567153492 37.0 31.0 39.0 18.0 40.0 54-55 32.84853448017366 37.0 31.5 40.0 16.0 41.0 56-57 32.54037914424946 37.0 31.0 40.0 13.5 41.0 58-59 32.200003452878654 36.0 31.0 39.5 10.5 41.0 60-61 31.74640832470871 36.0 30.5 39.0 8.0 41.0 62-63 31.200901201329177 35.0 29.5 39.0 6.5 40.0 64-65 30.68869073201936 35.0 29.0 38.5 2.0 40.0 66-67 30.14333081030884 34.5 28.0 38.0 2.0 40.0 68-69 29.5688699364216 34.0 28.0 37.5 2.0 40.0 70-71 28.92348920657392 34.0 26.5 37.0 2.0 39.0 72-73 28.369086831720324 34.0 26.0 36.0 2.0 39.0 74-75 27.744389753673453 33.0 25.5 36.0 2.0 38.5 76-77 27.00751546299014 32.5 24.5 35.0 2.0 37.5 78-79 26.22102103439158 32.0 22.0 35.0 2.0 37.0 80-81 25.560611650194314 31.5 20.0 35.0 2.0 36.0 82-83 24.897305936861386 31.0 18.0 34.5 2.0 36.0 84-85 24.242288040954776 31.0 13.0 34.0 2.0 35.0 86-87 23.568008079736053 30.5 7.0 34.0 2.0 35.0 88-89 22.772719260066275 29.5 2.0 34.0 2.0 35.0 90-91 21.952836403517935 29.0 2.0 34.0 2.0 35.0 92-93 21.104912537674995 29.0 2.0 34.0 2.0 35.0 94-95 20.159142722921935 27.5 2.0 34.0 2.0 35.0 96-97 18.945082419304864 25.5 2.0 33.0 2.0 35.0 98-99 17.507232872131723 22.0 2.0 32.5 2.0 34.0 100-101 14.257335777002194 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7413207161221855 1101 2 0.7120102621583158 1101 3 0.8752443869477524 1101 4 0.7815991906699509 1101 5 0.7511933292946793 1101 6 0.6287894486114638 1101 7 0.8075534064015031 1101 8 0.7827854966052215 1101 9 0.6449471635887036 1101 10-11 0.7501624946853624 1101 12-13 0.6843002968475105 1101 14-15 0.9177788923775694 1101 16-17 0.8993639721759834 1101 18-19 1.0153516888687761 1101 20-21 0.7584047155074884 1101 22-23 0.986701241554222 1101 24-25 1.0484042142127663 1101 26-27 1.1363805779428162 1101 28-29 1.1795917076080045 1101 30-31 1.0868288817119094 1101 32-33 1.081042450492113 1101 34-35 1.3009272513941426 1101 36-37 1.257863589031217 1101 38-39 0.9528218878125969 1101 40-41 1.261781636385301 1101 42-43 1.3022211299714357 1101 44-45 1.0463770309968368 1101 46-47 1.038791563354927 1101 48-49 1.0273917206717869 1101 50-51 0.8687810748184432 1101 52-53 0.8728563953920414 1101 54-55 0.9289992066076636 1101 56-57 0.8352762793796344 1101 58-59 0.9936585988011117 1101 60-61 1.027973881944888 1101 62-63 0.8337237577950134 1101 64-65 0.8323268709704159 1101 66-67 0.9882190888820546 1101 68-69 1.0407388965196667 1101 70-71 1.2713194216811026 1101 72-73 1.5072980094833355 1101 74-75 1.2995974673615258 1101 76-77 1.5429936096505728 1101 78-79 1.5374408309924554 1101 80-81 1.502478591160962 1101 82-83 1.2646222446183337 1101 84-85 1.6474429259680754 1101 86-87 1.8901761895096065 1101 88-89 2.0752416817554717 1101 90-91 1.8818534021861595 1101 92-93 1.8038111303973565 1101 94-95 1.7056347731865138 1101 96-97 1.624341143660203 1101 98-99 1.812852262490086 1101 100-101 1.5437548075559242 1102 1 0.6048399978413492 1102 2 0.7002323359338192 1102 3 0.637518603262297 1102 4 0.7399410156588146 1102 5 0.734828022047715 1102 6 0.6681237405583502 1102 7 0.6207162272917301 1102 8 0.48188556982216113 1102 9 0.6680388055352182 1102 10-11 0.6582672137814001 1102 12-13 0.754768300289463 1102 14-15 0.9156262576999126 1102 16-17 0.8468077008053214 1102 18-19 0.9129734592091197 1102 20-21 0.7653697600792455 1102 22-23 0.9019158351247185 1102 24-25 0.8073742330076357 1102 26-27 0.8707653669346058 1102 28-29 0.574175352302035 1102 30-31 0.7395942365121471 1102 32-33 0.7118233808756287 1102 34-35 0.8994592328559179 1102 36-37 0.898621742907558 1102 38-39 0.8409278146107582 1102 40-41 0.8245260073612002 1102 42-43 0.8351663946469117 1102 44-45 1.040739515240098 1102 46-47 0.9025943720171696 1102 48-49 0.9691262262309905 1102 50-51 0.982867410423772 1102 52-53 1.0403325998862059 1102 54-55 0.7675303766445722 1102 56-57 0.9781781045194649 1102 58-59 1.0938874960935934 1102 60-61 1.2057348965656303 1102 62-63 1.027629296618862 1102 64-65 0.8886638160879023 1102 66-67 1.0644590592933554 1102 68-69 0.9077567403184936 1102 70-71 0.8699533030092717 1102 72-73 0.7280999236911931 1102 74-75 0.6741375026868148 1102 76-77 0.9935523605846583 1102 78-79 0.962718036369747 1102 80-81 1.1743624366354766 1102 82-83 1.4770914859549613 1102 84-85 1.2182666354251417 1102 86-87 1.2427973748692374 1102 88-89 1.4360794873607183 1102 90-91 1.2157033112310884 1102 92-93 1.5438372092150168 1102 94-95 1.4869350154817518 1102 96-97 1.271823273442891 1102 98-99 1.3307918283211713 1102 100-101 0.7185097761179264 1103 1 0.7174094181815072 1103 2 0.4616433043475645 1103 3 0.6774859178521382 1103 4 0.5717847041928934 1103 5 0.5338416747146084 1103 6 0.529290291524454 1103 7 0.6645088918757835 1103 8 0.42760031007438926 1103 9 0.48960683746857825 1103 10-11 0.6801780086442974 1103 12-13 0.4669559087587416 1103 14-15 0.7340161396853304 1103 16-17 0.7858913523152111 1103 18-19 0.8078992662205806 1103 20-21 0.6643667807896563 1103 22-23 0.7577023097284439 1103 24-25 0.7459914666070802 1103 26-27 0.7837368467448584 1103 28-29 0.878652241113798 1103 30-31 0.9001540826303938 1103 32-33 0.8172302129941116 1103 34-35 0.8010812101786087 1103 36-37 0.887865205825527 1103 38-39 0.913855328205571 1103 40-41 1.0488802535720083 1103 42-43 1.0051088336148624 1103 44-45 1.0217863161194174 1103 46-47 0.8923367936300295 1103 48-49 0.9605354591362314 1103 50-51 1.0018350721153055 1103 52-53 0.9055233611959324 1103 54-55 1.078511806703581 1103 56-57 0.92135104794189 1103 58-59 0.7832350895218028 1103 60-61 0.6250301354873002 1103 62-63 0.8463120456643338 1103 64-65 0.8498245820256578 1103 66-67 0.9043728623709875 1103 68-69 1.0909830506274147 1103 70-71 1.2891473039348753 1103 72-73 1.3014287736275598 1103 74-75 1.2541010570082314 1103 76-77 1.1589354740869098 1103 78-79 1.2216522895225879 1103 80-81 1.1153117597871578 1103 82-83 1.1651952982886407 1103 84-85 1.2344864086520282 1103 86-87 1.1342485492299907 1103 88-89 1.0845341616636226 1103 90-91 1.3737358230903993 1103 92-93 1.4608654401533485 1103 94-95 1.2585420963564466 1103 96-97 1.3102684024490223 1103 98-99 1.6343286500265997 1103 100-101 1.4874340500968692 1104 1 0.698112120516825 1104 2 0.6047257065442295 1104 3 0.6898812499348956 1104 4 0.8514757299384783 1104 5 0.8023556621544614 1104 6 0.7927424977066551 1104 7 0.8359807674704882 1104 8 0.7375141969242947 1104 9 0.6968544110759467 1104 10-11 0.7294246035386749 1104 12-13 0.9227031289419827 1104 14-15 1.0046226358928152 1104 16-17 0.9761575758423859 1104 18-19 1.063537910431215 1104 20-21 0.934059131875749 1104 22-23 1.1047529173460973 1104 24-25 0.9648434158771195 1104 26-27 0.9662243409677345 1104 28-29 1.0483408540599939 1104 30-31 0.890913230878219 1104 32-33 0.9212210171699482 1104 34-35 0.9435748553407706 1104 36-37 1.1331152194187624 1104 38-39 1.1197248875128594 1104 40-41 0.9755167092716519 1104 42-43 0.8762585495162298 1104 44-45 1.0581125401903222 1104 46-47 1.0532832566700208 1104 48-49 1.0995721744666795 1104 50-51 0.8162007437571006 1104 52-53 0.9034240558102908 1104 54-55 1.0417234126084836 1104 56-57 0.9028905838688033 1104 58-59 0.9680614699149217 1104 60-61 1.0457161521254221 1104 62-63 1.287703109470982 1104 64-65 1.4273943832925475 1104 66-67 1.6547500746701473 1104 68-69 1.5079759549582192 1104 70-71 1.370427209054089 1104 72-73 1.5129624644630013 1104 74-75 1.4797062958413854 1104 76-77 1.4542578716618806 1104 78-79 1.6472607567492936 1104 80-81 1.4954245156798258 1104 82-83 1.541042326807247 1104 84-85 1.4218317855924631 1104 86-87 1.2445950408157778 1104 88-89 1.3511629371849203 1104 90-91 1.5513161591151956 1104 92-93 1.711314016941536 1104 94-95 1.164911886748751 1104 96-97 0.7651991788946617 1104 98-99 0.616398322157746 1104 100-101 9.763114676140106E-4 1105 1 0.4187804270187243 1105 2 0.331720143869628 1105 3 0.5621017537669069 1105 4 0.5298029016154828 1105 5 0.39046489285571084 1105 6 0.28808811507516197 1105 7 0.3805680837868479 1105 8 0.35754375323593734 1105 9 0.5312776947723492 1105 10-11 0.5248453391078769 1105 12-13 0.6168566744719541 1105 14-15 0.37302585861091586 1105 16-17 0.6146426430314378 1105 18-19 0.45510503320774376 1105 20-21 0.7039863212276245 1105 22-23 0.6413509465980027 1105 24-25 0.6220982138680782 1105 26-27 0.5917478362716224 1105 28-29 0.612222010192923 1105 30-31 0.5350284808548977 1105 32-33 0.6407134537981776 1105 34-35 0.6187009115370472 1105 36-37 0.5052252553085737 1105 38-39 0.6139498689787928 1105 40-41 0.578437236179191 1105 42-43 0.5500123483860193 1105 44-45 0.38908308698209026 1105 46-47 0.34044958211349297 1105 48-49 0.5402975315236205 1105 50-51 0.20703632327192878 1105 52-53 0.3288937900489728 1105 54-55 0.3246539061792504 1105 56-57 0.2931959172610519 1105 58-59 0.3190880853244593 1105 60-61 0.667083597959305 1105 62-63 0.4485493430051193 1105 64-65 0.48533972866443165 1105 66-67 0.5020465558979623 1105 68-69 0.4646504280261361 1105 70-71 0.6484001468630929 1105 72-73 0.45412846586717137 1105 74-75 0.16872185463733658 1105 76-77 0.33769087162751177 1105 78-79 0.681412534792198 1105 80-81 0.5890976484033779 1105 82-83 0.6615800356530279 1105 84-85 0.6097512569174377 1105 86-87 0.6079363769378112 1105 88-89 0.8952015960550064 1105 90-91 0.6641260216930718 1105 92-93 1.0253375539777565 1105 94-95 1.0876579725013293 1105 96-97 1.121885435209748 1105 98-99 1.2138715520930852 1105 100-101 1.3398649548721853 1106 1 0.3648412571814532 1106 2 0.5954867226351865 1106 3 0.3804837895047086 1106 4 0.5060955506824669 1106 5 0.6179787510657064 1106 6 0.26976997792996826 1106 7 0.3261285912130347 1106 8 0.5095315833110945 1106 9 0.6229754187498955 1106 10-11 0.3291859023885664 1106 12-13 0.3320550520315422 1106 14-15 0.5413451310102957 1106 16-17 0.50165930856285 1106 18-19 0.13412407628577228 1106 20-21 0.47929316421548407 1106 22-23 0.4276973256236971 1106 24-25 0.5657908227146251 1106 26-27 0.20627106195041733 1106 28-29 0.30532023238487227 1106 30-31 0.4290602063439479 1106 32-33 0.7700748571020881 1106 34-35 0.5590473586779865 1106 36-37 0.44292777654592896 1106 38-39 0.5013869840059968 1106 40-41 0.7353244989516057 1106 42-43 0.4238746615150646 1106 44-45 0.7140212156290957 1106 46-47 0.6333771918195907 1106 48-49 0.7593359063586007 1106 50-51 0.6289263755260563 1106 52-53 0.910522620315497 1106 54-55 0.6528254820093622 1106 56-57 0.4058447571934565 1106 58-59 0.030902999969562472 1106 60-61 0.7643274440707373 1106 62-63 0.8162962851168913 1106 64-65 0.8609041121727543 1106 66-67 0.5114233500050211 1106 68-69 0.258294058763628 1106 70-71 0.30868465777937004 1106 72-73 0.7595134629966012 1106 74-75 0.7394080509195398 1106 76-77 0.4757746760913335 1106 78-79 0.9736812916239934 1106 80-81 0.9050263187612408 1106 82-83 0.9609631871835163 1106 84-85 0.928543200505036 1106 86-87 0.9059361401562249 1106 88-89 0.8538795601225893 1106 90-91 1.0660919575804044 1106 92-93 0.9990746647882958 1106 94-95 0.9826112811185617 1106 96-97 1.409147315644777 1106 98-99 0.8384125850446473 1106 100-101 0.2849312212806634 1107 1 0.41698348182555023 1107 2 0.4506860835902877 1107 3 0.26593251420143815 1107 4 0.5137120742039016 1107 5 0.3939771039150912 1107 6 0.43743876442581353 1107 7 0.6536220738219711 1107 8 0.33810407341890425 1107 9 0.42701403390491777 1107 10-11 0.4278307185083534 1107 12-13 0.4455342861684315 1107 14-15 0.5076946489400882 1107 16-17 0.4252487036375001 1107 18-19 0.505154040803923 1107 20-21 0.5191460043118354 1107 22-23 0.7169247438699173 1107 24-25 0.5287183233183725 1107 26-27 0.5133152625560342 1107 28-29 0.447127670570282 1107 30-31 0.5362945104228203 1107 32-33 0.6085317989750081 1107 34-35 0.5579110724453216 1107 36-37 0.6806724496236924 1107 38-39 0.8656038753497839 1107 40-41 0.8719110579548968 1107 42-43 1.048950517135502 1107 44-45 1.1306496964732276 1107 46-47 1.1183430722711378 1107 48-49 1.0782375788976282 1107 50-51 0.8106261296919186 1107 52-53 0.7673892568463216 1107 54-55 0.6905977108023009 1107 56-57 0.7899900036777865 1107 58-59 0.60037126755104 1107 60-61 0.9052400955497646 1107 62-63 0.8857583604028179 1107 64-65 0.8859155679195148 1107 66-67 0.8854697364909541 1107 68-69 0.6213522168033947 1107 70-71 0.4092839171808258 1107 72-73 0.44616473148834146 1107 74-75 0.6493434343155187 1107 76-77 0.8452666292032696 1107 78-79 0.9170573930785615 1107 80-81 0.7721001804625125 1107 82-83 1.0238482705627767 1107 84-85 0.8567087019880937 1107 86-87 0.8265919352160118 1107 88-89 1.0012213624521316 1107 90-91 1.049366567310976 1107 92-93 0.9506826491341727 1107 94-95 1.1051986079664964 1107 96-97 1.111307962368123 1107 98-99 1.0433659570591907 1107 100-101 0.9841923256146501 1108 1 0.28731737408791247 1108 2 0.20787976655765306 1108 3 0.40985057214752985 1108 4 0.4860265879578449 1108 5 0.31299701964655213 1108 6 0.4086470130934856 1108 7 0.37533461657412204 1108 8 0.30620368019530986 1108 9 0.3087324668711702 1108 10-11 0.44397737891975453 1108 12-13 0.5359712719771608 1108 14-15 0.6655372174286214 1108 16-17 0.3899721669649949 1108 18-19 0.6125265021290431 1108 20-21 0.670709500967881 1108 22-23 0.4769847263768625 1108 24-25 0.3447686387704181 1108 26-27 0.5984743649347593 1108 28-29 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92-93 0.4059140663690002 2312 94-95 0.4322526891212668 2312 96-97 0.7234467006564778 2312 98-99 0.854858382019902 2312 100-101 0.8861283924554293 2313 1 -0.5664724571076256 2313 2 -0.5780075441830306 2313 3 -0.7572627490904722 2313 4 -0.622357756424087 2313 5 -0.6053989086653928 2313 6 -0.5498394472206485 2313 7 -0.5230139344493594 2313 8 -0.44547162632842685 2313 9 -0.7825757853066193 2313 10-11 -0.5943481168457083 2313 12-13 -0.6254267943651186 2313 14-15 -0.6448772705067753 2313 16-17 -0.6285114501488138 2313 18-19 -0.9281576535038027 2313 20-21 -0.5884309657955313 2313 22-23 -0.8469381299978807 2313 24-25 -0.923348669985657 2313 26-27 -0.8504652057982938 2313 28-29 -0.6283440275429228 2313 30-31 -0.6477363083997716 2313 32-33 -0.6545663405351476 2313 34-35 -0.6310766688601319 2313 36-37 -0.8268475781339575 2313 38-39 -0.6225389646044377 2313 40-41 -0.6036855775497045 2313 42-43 -0.7249561757591536 2313 44-45 -0.65105613962794 2313 46-47 -0.7172516325100631 2313 48-49 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2314 70-71 -0.5887087812151819 2314 72-73 -0.5824014796215238 2314 74-75 -0.5173773525182881 2314 76-77 -0.26880530111743184 2314 78-79 -0.46719982006205285 2314 80-81 -0.42268410389360866 2314 82-83 -0.1838804445794544 2314 84-85 -0.0354547925499169 2314 86-87 -0.2506007131573291 2314 88-89 -0.6518948933512796 2314 90-91 -0.4527018415276878 2314 92-93 -0.6452784468738209 2314 94-95 -0.5463398732487157 2314 96-97 -0.2163746097064383 2314 98-99 -0.1830128992691762 2314 100-101 0.26786134609410084 2315 1 -0.6728548155128635 2315 2 -0.7061523805694989 2315 3 -0.8666690864282032 2315 4 -0.8892998357011521 2315 5 -0.8261073333622093 2315 6 -0.9061455281303878 2315 7 -0.7052662970176513 2315 8 -0.9917873215927102 2315 9 -0.918281247046508 2315 10-11 -1.050606193784951 2315 12-13 -0.9919682981369533 2315 14-15 -1.2715789366784875 2315 16-17 -1.346837304897484 2315 18-19 -1.2929047715705124 2315 20-21 -1.2658554714156551 2315 22-23 -1.0987921816550354 2315 24-25 -1.0175571401099646 2315 26-27 -1.3721351337516552 2315 28-29 -1.481835299363368 2315 30-31 -1.5581292358147891 2315 32-33 -1.5637618807764397 2315 34-35 -1.6009745180381643 2315 36-37 -1.3248155456539834 2315 38-39 -1.7834282042568006 2315 40-41 -1.8554443670902643 2315 42-43 -1.832622708177432 2315 44-45 -1.5714871854500387 2315 46-47 -1.5582605159566967 2315 48-49 -2.009994528084775 2315 50-51 -2.002585623657694 2315 52-53 -1.6765138498808518 2315 54-55 -1.603071703426565 2315 56-57 -1.4263954905626015 2315 58-59 -1.4476921787503017 2315 60-61 -1.8548875299262804 2315 62-63 -2.1830757721046794 2315 64-65 -1.7056461937711695 2315 66-67 -1.550331483728936 2315 68-69 -1.5677091492212227 2315 70-71 -1.6610049119185248 2315 72-73 -1.4607496577814807 2315 74-75 -1.12784131963722 2315 76-77 -1.236243788577319 2315 78-79 -1.3198410697409457 2315 80-81 -1.2653341068894335 2315 82-83 -1.4085758863120468 2315 84-85 -1.50162573635858 2315 86-87 -1.7618119835177417 2315 88-89 -1.890229256284048 2315 90-91 -2.336546152319336 2315 92-93 -2.4021046222528284 2315 94-95 -2.304236281083039 2315 96-97 -2.100600802027351 2315 98-99 -1.958070560280964 2315 100-101 -1.8844921666994487 2316 1 -0.7842317404322046 2316 2 -0.6621740495608606 2316 3 -0.671018382679101 2316 4 -0.7742752502720691 2316 5 -0.8223687983438239 2316 6 -0.9009177782959412 2316 7 -0.6361488364318291 2316 8 -0.7661797304011984 2316 9 -0.8779209894982856 2316 10-11 -0.9835489076218309 2316 12-13 -0.8583919283270163 2316 14-15 -1.4027960915314708 2316 16-17 -1.0618097607634596 2316 18-19 -1.1540742349177933 2316 20-21 -1.2573999153904651 2316 22-23 -1.0261422387157708 2316 24-25 -1.0014550929679302 2316 26-27 -1.2916837199133724 2316 28-29 -1.3796152139835982 2316 30-31 -1.3158764637900475 2316 32-33 -0.980434535298329 2316 34-35 -1.046817537607886 2316 36-37 -1.107026332246818 2316 38-39 -1.2086402447769728 2316 40-41 -1.3382039230027445 2316 42-43 -1.3593169189781094 2316 44-45 -1.0015282550923104 2316 46-47 -1.1248511016393863 2316 48-49 -1.1130131115695647 2316 50-51 -1.2660026460734102 2316 52-53 -0.9880210987079394 2316 54-55 -1.230370687604541 2316 56-57 -1.2269904015901147 2316 58-59 -1.2873173390134909 2316 60-61 -1.4063052427064804 2316 62-63 -1.1564611645557576 2316 64-65 -1.3456598601701444 2316 66-67 -1.132206950429417 2316 68-69 -1.3385578044193878 2316 70-71 -1.4142915399811393 2316 72-73 -1.2263902878447368 2316 74-75 -0.8550850042110945 2316 76-77 -1.1332903670464027 2316 78-79 -0.9861914367612492 2316 80-81 -1.1012041774756796 2316 82-83 -0.9660483774007105 2316 84-85 -0.8612383573484799 2316 86-87 -1.4877094573472895 2316 88-89 -1.6050386311519595 2316 90-91 -1.383559269244536 2316 92-93 -1.5463316253665127 2316 94-95 -1.4187409797882502 2316 96-97 -2.0527201505487014 2316 98-99 -1.9217094855666197 2316 100-101 -1.7647857829413276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4868.0 3 1774.0 4 1891.0 5 2106.0 6 2334.0 7 2918.0 8 3491.0 9 4420.0 10 5474.0 11 6495.0 12 7641.0 13 8860.0 14 9701.0 15 10944.0 16 12234.0 17 14202.0 18 13827.0 19 13530.0 20 14340.0 21 15635.0 22 17172.0 23 19032.0 24 21492.0 25 23820.0 26 26666.0 27 30084.0 28 34207.0 29 39084.0 30 44564.0 31 51352.0 32 60587.0 33 72494.0 34 87088.0 35 106341.0 36 125158.0 37 123943.0 38 57824.0 39 2938.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.52891881903368 18.328026376395236 14.054788185967423 23.08826661860366 2 16.00775637773887 19.69276307775105 40.1110729292235 24.18840761528657 3 19.983090985950064 24.018527188512778 28.757051104313163 27.241330721223996 4 13.212017404617352 15.768343306622738 36.01057514150798 35.009064147251934 5 14.20679622684396 37.37261052392317 33.59399931810433 14.826593931128537 6 33.608806660095794 34.788926026819325 17.261075609835935 14.341191703248942 7 30.14492938055961 31.828015438287753 19.83489103935745 18.192164141795182 8 27.37067339550112 34.7000975329526 19.18463178810716 18.744597283439123 9 27.26908287654422 16.439953821120472 19.32004326997373 36.970920032361576 10-11 25.809067335728685 26.205686572625275 26.829296007672397 21.155950083973647 12-13 26.769618989744913 23.316533169831335 27.193766683316845 22.720081157106904 14-15 23.446797061480932 24.810616045506688 25.36863289054362 26.373954002468764 16-17 23.20080715532286 28.070053446771382 25.924001963350783 22.80513743455497 18-19 23.154659530138137 26.66494253082537 28.014495467552912 22.16590247148358 20-21 23.800670661665873 25.849020667597916 28.074195526467587 22.276113144268624 22-23 23.56434824467183 25.75193446786963 27.90720190694463 22.77651538051391 24-25 23.225433352564654 26.022249227905693 28.057586664829913 22.694730754699737 26-27 22.927217541867495 26.251797275913844 27.958135460623122 22.86284972159554 28-29 22.911283258948558 26.47611619206317 27.939675834198084 22.672924714790184 30-31 23.0336823271426 26.121759321553125 27.891608874794986 22.952949476509286 32-33 22.796845694877163 26.163886406468617 27.946319719770862 23.092948178883358 34-35 23.445500232734044 26.316129196485715 27.668895822423924 22.569474748356317 36-37 23.40923791328996 25.927875421761552 27.65496711589414 23.00791954905434 38-39 22.979911936635098 26.329710001676922 27.693833246760562 22.996544814927418 40-41 23.31138239128785 26.09455158865228 27.832282744978105 22.761783275081765 42-43 23.118880089284577 26.018175010133582 27.645819056949822 23.217125843632022 44-45 23.124968479706386 26.294762792468568 27.646171244132685 22.934097483692366 46-47 23.31779352962559 26.49700109051254 27.147764449291166 23.037440930570703 48-49 23.200762265841576 26.472652985927436 27.18021162237307 23.146373125857924 50-51 23.271133518472745 26.59859945912151 27.10240160227983 23.027865420125913 52-53 23.454143540974222 26.440373767229257 27.10221347806628 23.003269213730242 54-55 23.17935771625175 26.505598183935593 27.22660774271936 23.088436357093297 56-57 23.11404126549955 26.72261423891461 27.06875008519358 23.094594410392254 58-59 23.282624500181754 26.25849691021447 27.140903307888042 23.31797528171574 60-61 23.028325518775613 26.487056793258105 27.414307569905038 23.070310118061236 62-63 23.09187097735643 26.445107583992204 27.44084675085348 23.02217468779788 64-65 22.87783813648499 26.467570775077288 27.335164086225916 23.319427002211807 66-67 22.88425295732976 26.569533824146784 27.110522078427167 23.435691140096285 68-69 23.065535894004828 26.28274011690911 27.355913951623346 23.295810037462715 70-71 22.994000166321225 26.31678921874922 27.166208980346372 23.52300163458318 72-73 23.03738842252639 26.341894782379903 27.06828725425463 23.55242954083907 74-75 22.874853506727444 26.62639909879656 27.06173234457853 23.43701504989746 76-77 22.97034984582865 26.29782469813222 27.123661508234743 23.608163947804385 78-79 23.129742829700213 26.38524021855977 27.044545229023413 23.440471722716605 80-81 23.055232836828615 26.605567946844022 26.866867885642314 23.472331330685044 82-83 23.046970839732115 26.53416267594755 27.014412023953398 23.40445446036693 84-85 23.003031873977996 26.406563714106962 26.924612973767225 23.665791438147817 86-87 22.981614526632217 26.285311068178558 27.01057037496115 23.722504030228073 88-89 23.07651755569563 26.29152830148378 26.85038199924832 23.781572143572273 90-91 22.93416281633963 26.344054255751402 26.873404155717136 23.848378772191836 92-93 23.05426787067034 26.07347173225566 26.977928669553524 23.894331727520477 94-95 22.931689947212583 25.957774611593237 27.05987951834607 24.05065592284811 96-97 23.106776436410176 25.751667316595174 26.97625988414002 24.165296362854633 98-99 22.91485748536409 25.74860911731947 26.837048942983692 24.499484454332745 100-101 23.436940610998928 25.265970634167157 27.040457741977892 24.256631012856026 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 210.0 1 139.5 2 125.0 3 188.0 4 175.5 5 172.0 6 253.0 7 488.5 8 753.0 9 862.5 10 803.5 11 713.0 12 724.0 13 765.5 14 806.5 15 842.5 16 931.5 17 1000.0 18 1023.5 19 1142.5 20 1287.5 21 1481.5 22 1715.5 23 1998.0 24 2285.5 25 2759.5 26 3537.0 27 4427.0 28 5178.0 29 6134.5 30 7626.0 31 9668.0 32 12493.5 33 14698.5 34 16417.0 35 18792.0 36 22040.0 37 25194.5 38 27526.0 39 29576.5 40 31495.0 41 33721.0 42 35755.5 43 37792.5 44 40112.0 45 42119.5 46 43167.0 47 44391.5 48 45935.0 49 46073.5 50 44803.0 51 43134.0 52 41714.0 53 41100.0 54 40877.5 55 38587.5 56 35131.0 57 32164.5 58 29653.0 59 26314.5 60 21772.0 61 17975.5 62 14766.0 63 11357.0 64 8478.0 65 6492.5 66 4814.5 67 3576.0 68 2709.5 69 2001.0 70 1518.5 71 1134.5 72 835.0 73 651.5 74 483.0 75 345.0 76 249.0 77 168.5 78 111.0 79 67.0 80 44.5 81 29.5 82 19.0 83 13.5 84 9.0 85 6.5 86 4.0 87 2.0 88 0.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 1.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17927709442078416 2 0.04843116640966951 3 0.04779510981517104 4 0.053883080076799296 5 0.05960758942728556 6 0.06605902060005579 7 0.03716387816426798 8 0.035437438836343545 9 0.04188887000911378 10-11 0.043933337634287445 12-13 0.0402078632950821 14-15 0.034983112697416066 16-17 0.03371099950841912 18-19 0.042070600464684776 20-21 0.05202034290719662 22-23 0.05011217312370119 24-25 0.06928473618644092 26-27 0.0547462997407615 28-29 0.04611410310113936 30-31 0.055155193265796244 32-33 0.052156640748874855 34-35 0.051157123243234404 36-37 0.04829486856799127 38-39 0.02730500094954163 40-41 0.024942505027118728 42-43 0.020808137162878645 44-45 0.004497828775382066 46-47 0.011903344839900012 48-49 0.011585316542650775 50-51 0.008995657550764131 52-53 0.010313203353653827 54-55 0.011767046998221767 56-57 0.008723061867407643 58-59 0.011903344839900012 60-61 0.011585316542650775 62-63 0.003816339566990844 64-65 0.006633161628341228 66-67 0.004088935250347332 68-69 0.009404551075798864 70-71 0.022761739560266815 72-73 0.02094443500455689 74-75 0.020399243637843914 76-77 0.011994210067685508 78-79 0.004906722300416799 80-81 0.006496863786662984 82-83 0.005542778894915273 84-85 0.00513388536988054 86-87 0.008813927095193139 88-89 0.03021268823867751 90-91 0.03707301293648248 92-93 0.02339779615476529 94-95 0.033029510300027896 96-97 0.03725474339205347 98-99 0.021853087282411855 100-101 0.12689329060244556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1100531.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.24337676401963 #Duplication Level Percentage of deduplicated Percentage of total 1 84.33304134464812 56.70838472793819 2 9.319020629574466 12.532848305322943 3 2.6702866762517834 5.386772791174211 4 1.1464392288992544 3.083617800236988 5 0.6440233800682518 2.1653153395383433 6 0.3858829386018191 1.5568843096325506 7 0.2649859251448634 1.2472983881174844 8 0.19596222340659397 1.0541729296035667 9 0.15105735453887142 0.914184594381008 >10 0.8235818843065401 9.816417007799132 >50 0.044006801733628594 2.052644676954891 >100 0.019811849820771277 2.3352994656335326 >500 0.0014926709325367618 0.5701142885592136 >1k 4.07092072510026E-4 0.5760453751079251 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2663 0.24197410159277655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2168 0.19699581383895592 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1482 0.13466226757810548 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.086522778549628E-5 2 0.0 0.0 0.0 0.0 9.086522778549628E-5 3 0.0 0.0 0.0 0.0 9.086522778549628E-5 4 0.0 0.0 0.0 0.0 9.086522778549628E-5 5 0.0 0.0 0.0 0.0 9.086522778549628E-5 6 0.0 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 7 0.0 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 8 0.0 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 9 0.0 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 10-11 0.0 0.0 0.0 1.8173045557099256E-4 9.086522778549628E-5 12-13 0.0 0.0 0.0 2.7259568335648884E-4 1.8173045557099256E-4 14-15 9.086522778549628E-5 0.0 0.0 2.7259568335648884E-4 1.8173045557099256E-4 16-17 1.8173045557099256E-4 0.0 0.0 2.7259568335648884E-4 1.8173045557099256E-4 18-19 2.7259568335648884E-4 0.0 0.0 2.7259568335648884E-4 1.8173045557099256E-4 20-21 3.634609111419851E-4 0.0 0.0 3.18028297249237E-4 1.8173045557099256E-4 22-23 3.634609111419851E-4 0.0 0.0 8.177870500694665E-4 1.8173045557099256E-4 24-25 7.269218222839702E-4 0.0 0.0 0.001317545802889696 1.8173045557099256E-4 26-27 7.269218222839702E-4 0.0 0.0 0.0018173045557099256 1.8173045557099256E-4 28-29 8.177870500694664E-4 0.0 0.0 0.0039980700225618355 1.8173045557099256E-4 30-31 8.177870500694664E-4 0.0 0.0 0.011948777453792761 1.8173045557099256E-4 32-33 8.177870500694664E-4 0.0 0.0 0.02907687289135881 1.8173045557099256E-4 34-35 9.086522778549628E-4 0.0 0.0 0.0573813913465409 1.8173045557099256E-4 36-37 9.99517505640459E-4 0.0 0.0 0.10785702538138407 1.8173045557099256E-4 38-39 0.0010903827334259554 0.0 0.0 0.18245737739327653 1.8173045557099256E-4 40-41 0.0010903827334259554 0.0 0.0 0.26350916057793916 1.8173045557099256E-4 42-43 0.0011358153473187034 0.0 0.0 0.3408354694233965 1.8173045557099256E-4 44-45 0.001272113188996948 0.0 0.0 0.44092351782912065 1.8173045557099256E-4 46-47 0.0014084110306751925 0.0 0.0 0.5614562424865814 2.271630694637407E-4 48-49 0.0014538436445679405 0.0 0.0 0.6938468793700496 2.7259568335648884E-4 50-51 0.0014538436445679405 0.0 0.0 0.8329161104957516 3.634609111419851E-4 52-53 0.0014538436445679405 0.0 0.0 0.9701680370657437 4.5432613892748135E-4 54-55 0.0014538436445679405 0.0 0.0 1.1211406130313457 4.5432613892748135E-4 56-57 0.0014538436445679405 0.0 0.0 1.3009174662049503 4.997587528202295E-4 58-59 0.0014538436445679405 9.086522778549628E-5 0.0 1.4869185874818611 5.451913667129777E-4 60-61 0.0014538436445679405 9.086522778549628E-5 0.0 1.7018148511945599 5.451913667129777E-4 62-63 0.0014538436445679405 9.086522778549628E-5 0.0 1.9080789182676363 5.451913667129777E-4 64-65 0.0014538436445679405 9.086522778549628E-5 0.0 2.1250650822194013 5.451913667129777E-4 66-67 0.0014538436445679405 9.086522778549628E-5 0.0 2.378215606829794 5.451913667129777E-4 68-69 0.0014538436445679405 9.086522778549628E-5 0.0 2.650356964047355 5.451913667129777E-4 70-71 0.0016355741001389328 9.086522778549628E-5 0.0 2.9505302440367425 5.451913667129777E-4 72-73 0.0017264393279244291 9.086522778549628E-5 0.0 3.2591994228240733 5.451913667129777E-4 74-75 0.0017718719418171771 9.086522778549628E-5 0.0 3.56482461648059 5.451913667129777E-4 76-77 0.0018173045557099254 9.086522778549628E-5 0.0 3.903888213962169 5.451913667129777E-4 78-79 0.0018173045557099254 9.086522778549628E-5 0.0 4.278389250280092 5.451913667129777E-4 80-81 0.001999035011280918 9.086522778549628E-5 0.0 4.682012592103266 5.451913667129777E-4 82-83 0.001999035011280918 9.086522778549628E-5 0.0 5.088361890760006 5.451913667129777E-4 84-85 0.001999035011280918 9.086522778549628E-5 0.0 5.504888094928721 5.451913667129777E-4 86-87 0.001999035011280918 9.086522778549628E-5 0.0 5.94181354273528 5.451913667129777E-4 88-89 0.001999035011280918 9.086522778549628E-5 0.0 6.404453850005134 5.451913667129777E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2540 0.0 39.743664 1 GTATCAA 4110 0.0 35.915806 1 AATACCG 65 0.005831976 29.243055 5 ATCAACG 4985 0.0 28.889004 3 TATCAAC 5055 0.0 28.771029 2 TCAACGC 5060 0.0 28.460808 4 CAACGCA 5155 0.0 28.023413 5 AACGCAG 5315 0.0 27.26426 6 TACCTGG 1045 0.0 25.924694 2 TACATGG 5095 0.0 25.466753 2 GTACATG 5125 0.0 25.36498 1 ACATGGG 5140 0.0 24.411581 3 ACGCAGA 6080 0.0 23.75458 7 GTACCTG 1245 0.0 23.713085 1 CGCAGAG 6180 0.0 23.370203 8 GGACCGA 350 0.0 23.07693 6 GAGTACT 2905 0.0 22.72727 12-13 GCAGAGT 6600 0.0 21.449156 9 GTACTTT 3195 0.0 20.813528 14-15 CATGGGA 2625 0.0 20.278873 4 >>END_MODULE