FastQCFastQC Report
Thu 26 May 2016
SRR938442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100531
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26140.23752170543128726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22520.20462849297293761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16810.15274444790741923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.037.268781
GTATCAA37600.036.6871031
TATCAAC48250.028.3921622
ATCAACG48450.028.1601263
TCAACGC48950.027.8712164
CAACGCA49850.027.2726655
AACGCAG52150.026.2294886
GTACATG52650.025.7483731
TACATGG52250.025.6723792
ACATGGG51950.025.3478223
TAAGGTG5850.023.5650775
GTGTAGG4950.023.0626561
ACGCAGA59600.022.7117317
CGCAGAG60150.022.5830238
GGGCGTA1754.0523773E-621.7121899
GTACACG2005.3134136E-721.4050271
CATGGGA26500.020.987794
CATGGGG25400.020.9609534
CTATTAG3001.6370905E-1020.612251
AGGTAAG5450.020.0740322
Produced by FastQC (version 0.11.5)