##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938442_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1100531 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.750351421268462 31.0 31.0 34.0 30.0 34.0 2 31.980110510290032 31.0 31.0 34.0 30.0 34.0 3 32.04365165542815 33.0 31.0 34.0 30.0 34.0 4 35.72162528815635 37.0 35.0 37.0 33.0 37.0 5 35.591878829401445 37.0 35.0 37.0 33.0 37.0 6 35.61763548686952 37.0 35.0 37.0 33.0 37.0 7 35.54843979860631 37.0 35.0 37.0 33.0 37.0 8 35.538958012086894 37.0 35.0 37.0 33.0 37.0 9 37.10684206078702 39.0 37.0 39.0 33.0 39.0 10-11 37.017935887312575 39.0 37.0 39.0 33.0 39.0 12-13 36.90596766470004 39.0 37.0 39.0 33.0 39.0 14-15 37.955488305190855 40.0 37.0 41.0 33.0 41.0 16-17 37.87176871891842 40.0 37.0 41.0 33.0 41.0 18-19 37.969467466159514 40.0 37.0 41.0 33.0 41.0 20-21 38.0198245210721 40.0 37.0 41.0 33.0 41.0 22-23 37.92232158839687 40.0 37.0 41.0 32.5 41.0 24-25 37.834794294754076 40.0 37.0 41.0 32.0 41.0 26-27 37.647414293645525 40.0 37.0 41.0 32.0 41.0 28-29 37.532186735312315 39.5 36.5 41.0 32.0 41.0 30-31 37.307404789142694 39.0 36.0 41.0 31.0 41.0 32-33 37.11955365182807 39.0 36.0 41.0 31.0 41.0 34-35 36.99046051406094 39.0 36.0 40.5 30.5 41.0 36-37 37.01232223353999 39.0 36.0 41.0 31.0 41.0 38-39 36.90998027315905 39.0 35.5 41.0 30.0 41.0 40-41 36.748428713048526 39.0 35.0 41.0 30.0 41.0 42-43 36.55850675719266 39.0 35.0 40.0 30.0 41.0 44-45 36.320155452231695 39.0 35.0 40.0 30.0 41.0 46-47 35.98876360593205 38.0 35.0 40.0 29.0 41.0 48-49 35.6578351722941 38.0 34.5 40.0 28.0 41.0 50-51 35.29969214860826 38.0 34.0 40.0 27.0 41.0 52-53 34.80694637406852 38.0 33.5 40.0 26.0 41.0 54-55 34.49824448379918 38.0 33.0 40.0 26.0 41.0 56-57 34.16388952242145 37.0 33.0 40.0 25.0 41.0 58-59 33.71693573375035 37.0 32.0 39.5 23.5 41.0 60-61 33.344127062299926 36.0 32.0 39.0 23.0 40.5 62-63 32.874064883224555 36.0 31.0 39.0 22.0 40.0 64-65 32.36031970021744 35.0 31.0 38.5 20.5 40.0 66-67 31.85224950501167 35.0 30.5 38.0 19.5 40.0 68-69 31.314922069437387 34.5 30.0 37.5 18.0 39.5 70-71 30.732460966569775 34.0 29.0 37.0 15.0 39.0 72-73 30.281958890753646 34.0 29.0 36.0 12.5 39.0 74-75 29.749676746952154 34.0 28.5 36.0 8.0 38.5 76-77 28.493101057580386 31.5 26.5 34.5 7.5 36.5 78-79 29.191535267975187 33.5 28.5 35.0 7.0 37.0 80-81 28.91474978896551 33.0 28.0 35.0 4.0 37.0 82-83 28.44125381293212 33.0 27.0 35.0 2.0 36.0 84-85 27.91828126604339 33.0 26.0 35.0 2.0 36.0 86-87 27.34616244340232 32.5 26.0 35.0 2.0 35.0 88-89 26.71353146799136 32.0 25.0 34.0 2.0 35.0 90-91 26.173058278231146 32.0 24.5 34.0 2.0 35.0 92-93 25.417016422072617 31.0 21.5 34.0 2.0 35.0 94-95 24.649364715759937 31.0 19.0 34.0 2.0 35.0 96-97 23.64144671981071 31.0 6.0 34.0 2.0 35.0 98-99 22.398479915604376 30.0 2.0 34.0 2.0 35.0 100-101 18.741230824029493 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2748978181527022 1101 2 0.3690925420550073 1101 3 0.35177503783899766 1101 4 0.09930463424637992 1101 5 0.021178930850922484 1101 6 0.1343518634181109 1101 7 0.2992656545284831 1101 8 0.3032428469545394 1101 9 0.31748112169650966 1101 10-11 0.3646230736570928 1101 12-13 0.4380482876890426 1101 14-15 0.5877540253998603 1101 16-17 0.512576129234894 1101 18-19 0.5190402558558915 1101 20-21 0.45399052735906054 1101 22-23 0.4523154647907006 1101 24-25 0.3278496680116447 1101 26-27 0.44851694260292163 1101 28-29 0.39537074961844354 1101 30-31 0.31476708690567534 1101 32-33 0.17412832034121095 1101 34-35 7.293220389428257E-4 1101 36-37 0.3144294252002311 1101 38-39 0.36780339760809255 1101 40-41 0.35365644314363465 1101 42-43 0.2515801286433472 1101 44-45 0.41356228332129774 1101 46-47 0.3239049191598937 1101 48-49 0.3987010636116537 1101 50-51 0.4376062115866688 1101 52-53 0.5209175962276404 1101 54-55 0.40492162000753495 1101 56-57 0.42577552998137236 1101 58-59 0.4884038426000643 1101 60-61 0.5018348074921661 1101 62-63 0.5453035998697615 1101 64-65 0.38788362587249736 1101 66-67 0.268737183134629 1101 68-69 0.17045405383020196 1101 70-71 -0.20559047787616436 1101 72-73 -0.0594533353368476 1101 74-75 0.22355156327533265 1101 76-77 0.19536109961446257 1101 78-79 0.2920858260854047 1101 80-81 0.2607101434462855 1101 82-83 0.20979410107862506 1101 84-85 0.5387924862527775 1101 86-87 -0.00513165032438323 1101 88-89 0.09489684746623084 1101 90-91 -0.1679625373560576 1101 92-93 0.04816819324553734 1101 94-95 0.07818808760099571 1101 96-97 0.2900997123432454 1101 98-99 0.32625974505189603 1101 100-101 0.08398511410847931 1102 1 0.2369372873676241 1102 2 0.19393516337245842 1102 3 0.31684319752741175 1102 4 0.10301831826621566 1102 5 -0.07001861902340067 1102 6 0.0919501967891847 1102 7 0.17976636027940884 1102 8 0.3233114595312472 1102 9 0.275667266333933 1102 10-11 0.33759649288823823 1102 12-13 0.30703666733020185 1102 14-15 0.4189583119875735 1102 16-17 0.5000113095976602 1102 18-19 0.5059824484390916 1102 20-21 0.4318523096254623 1102 22-23 0.35306829722957644 1102 24-25 0.4156444228103737 1102 26-27 0.4431884287558603 1102 28-29 0.22565227630488494 1102 30-31 0.22350584961484543 1102 32-33 0.2831073969019755 1102 34-35 0.3325902966545584 1102 36-37 0.24110586670592937 1102 38-39 0.22727876425891225 1102 40-41 0.2915404546771896 1102 42-43 0.17051642613056828 1102 44-45 0.3255967518946292 1102 46-47 0.20940390770937256 1102 48-49 0.49227580527662695 1102 50-51 0.354974855110747 1102 52-53 0.5056392208889235 1102 54-55 0.3838538226297601 1102 56-57 0.13202557715722918 1102 58-59 0.3215291303728307 1102 60-61 0.4710006627339638 1102 62-63 0.4660750384694339 1102 64-65 0.3748451605599712 1102 66-67 0.4429232107759056 1102 68-69 0.31089660117590157 1102 70-71 0.35605705426003453 1102 72-73 0.2681608679949008 1102 74-75 0.2713727931689789 1102 76-77 0.4029830209175742 1102 78-79 0.3379030252732278 1102 80-81 0.3312904254447311 1102 82-83 0.08771711761659517 1102 84-85 -0.004391356202479102 1102 86-87 0.11057470445321016 1102 88-89 0.2708481959411273 1102 90-91 0.2663214839900796 1102 92-93 0.24049192534701547 1102 94-95 -0.033127609507314304 1102 96-97 0.16072273567453976 1102 98-99 0.30113010577742116 1102 100-101 0.11159573207456219 1103 1 0.15215769694762926 1103 2 0.16119913421591292 1103 3 0.20023871991546827 1103 4 0.19677916519436423 1103 5 0.1274822487308498 1103 6 0.02630458762569532 1103 7 0.25270833644508883 1103 8 0.15435970666765542 1103 9 0.36751182262226223 1103 10-11 0.28950844475679816 1103 12-13 0.23577596502775577 1103 14-15 0.38087474725310244 1103 16-17 0.42330183372123287 1103 18-19 0.3606625363715139 1103 20-21 0.31675193938365 1103 22-23 0.32329709510068483 1103 24-25 0.48357511817077636 1103 26-27 0.2960064606892203 1103 28-29 0.3796569621778474 1103 30-31 0.5072723369456327 1103 32-33 0.5763649463639666 1103 34-35 0.4884389603130117 1103 36-37 0.433338743022027 1103 38-39 0.49169071612747217 1103 40-41 0.5374873191918255 1103 42-43 0.4643416314990745 1103 44-45 0.5273459998409393 1103 46-47 0.29126929009974134 1103 48-49 0.6530128178879906 1103 50-51 0.521113637940779 1103 52-53 0.643673612882445 1103 54-55 0.645893912876204 1103 56-57 0.5672404909603088 1103 58-59 0.5873475672581705 1103 60-61 0.6073501652215327 1103 62-63 0.6200471835579506 1103 64-65 0.5468376689482639 1103 66-67 0.512990750981853 1103 68-69 0.5099413483701589 1103 70-71 0.38823627443820996 1103 72-73 0.21748763532105997 1103 74-75 0.19571908496580548 1103 76-77 0.07958908941231257 1103 78-79 0.23709529069032698 1103 80-81 0.41654004941788614 1103 82-83 0.7253248637695542 1103 84-85 0.3577369557262493 1103 86-87 0.4642532593531321 1103 88-89 0.5452103965659099 1103 90-91 0.26686021475486044 1103 92-93 0.04798426018445667 1103 94-95 0.28257223429692147 1103 96-97 -0.027207525808741195 1103 98-99 0.2596514525320224 1103 100-101 0.17582039365040458 1104 1 0.34549089434860036 1104 2 0.2716186894452335 1104 3 0.252407860551088 1104 4 0.21936092850894084 1104 5 0.241356185060134 1104 6 0.27791198778337645 1104 7 0.30528527416088025 1104 8 0.3843844463571884 1104 9 0.34611776830369223 1104 10-11 0.40664645688197254 1104 12-13 0.43877990026958 1104 14-15 0.5701767006011167 1104 16-17 0.49488041621858514 1104 18-19 0.6423058906384327 1104 20-21 0.6221851558151528 1104 22-23 0.6906508998474408 1104 24-25 0.5976852369756571 1104 26-27 0.5859612291964069 1104 28-29 0.6954414278277454 1104 30-31 0.5809978011546519 1104 32-33 0.6237849501700765 1104 34-35 0.5642297468176452 1104 36-37 0.728170084946278 1104 38-39 0.6286374714220457 1104 40-41 0.644573982867982 1104 42-43 0.5156603453070829 1104 44-45 0.5245075622474857 1104 46-47 0.5619344071375068 1104 48-49 0.5584293826053823 1104 50-51 0.6064468761214599 1104 52-53 0.8313953525447886 1104 54-55 0.6377668568992547 1104 56-57 0.5945102215423717 1104 58-59 0.5519295412679028 1104 60-61 0.522457857633789 1104 62-63 0.6945321698287756 1104 64-65 0.5539392851840219 1104 66-67 0.5191850187142286 1104 68-69 0.6996901793518653 1104 70-71 0.7822124319022556 1104 72-73 0.4256009281465474 1104 74-75 0.8331976756191004 1104 76-77 0.6033526146821373 1104 78-79 0.7719797821673673 1104 80-81 0.5530705118597758 1104 82-83 0.5299535305449687 1104 84-85 0.4423140701541115 1104 86-87 0.68594959325738 1104 88-89 0.3765557191333997 1104 90-91 0.42855090746616753 1104 92-93 0.5242054425241278 1104 94-95 0.7894223756665788 1104 96-97 0.38823999750366056 1104 98-99 0.5895932734819205 1104 100-101 0.32853615228890476 1105 1 0.21558550400399312 1105 2 0.2205628614020867 1105 3 0.1772430178328932 1105 4 0.14092132501520638 1105 5 -0.005409559076476 1105 6 0.18947626294183806 1105 7 0.05635435835709757 1105 8 0.006051383056600912 1105 9 0.06859283937139793 1105 10-11 0.04114363135749244 1105 12-13 0.10803151678380374 1105 14-15 0.12963054709486954 1105 16-17 0.08505539743902801 1105 18-19 0.11734995507315915 1105 20-21 0.260942710594982 1105 22-23 0.27704419517980483 1105 24-25 0.22954959216200876 1105 26-27 0.027507731290391746 1105 28-29 0.12226132580445892 1105 30-31 0.14745040549775013 1105 32-33 0.22949417890745138 1105 34-35 0.274462515046082 1105 36-37 0.2998539600635084 1105 38-39 0.16504647775062864 1105 40-41 0.3555579331033982 1105 42-43 0.3194029991889309 1105 44-45 0.1493903434865942 1105 46-47 -0.2642700616589835 1105 48-49 -0.23419283176134087 1105 50-51 -0.31660729244770636 1105 52-53 -0.18069720486172258 1105 54-55 -0.2950440858096215 1105 56-57 -0.27923719473519526 1105 58-59 -0.19209291563732478 1105 60-61 -0.11884659692461241 1105 62-63 -0.062008759771231325 1105 64-65 -0.21322866045593258 1105 66-67 -0.26471145564002185 1105 68-69 -0.30851145164593063 1105 70-71 -0.11667012349127859 1105 72-73 -0.17591051878710218 1105 74-75 -0.12600283106052856 1105 76-77 -0.32011764980266477 1105 78-79 -0.026176876103733093 1105 80-81 0.057680707314386126 1105 82-83 -0.13199860983278455 1105 84-85 0.14604273142994018 1105 86-87 0.326435357621893 1105 88-89 0.14970979981918475 1105 90-91 0.156399630211272 1105 92-93 0.02042621147919732 1105 94-95 -0.0028524783929988473 1105 96-97 -0.34403603488063084 1105 98-99 -0.5027260132388527 1105 100-101 -1.0939128974413066 1106 1 0.12702837963527358 1106 2 0.19350488883631556 1106 3 0.254117565758456 1106 4 0.14938302316085128 1106 5 0.04240896892051893 1106 6 0.05322548853630593 1106 7 0.19092901105040028 1106 8 0.08710123368958733 1106 9 0.19757068894048757 1106 10-11 0.03236568383218952 1106 12-13 0.15501157847675984 1106 14-15 0.30553899371034987 1106 16-17 0.2225367062897945 1106 18-19 0.2490893104433951 1106 20-21 0.3100233979751721 1106 22-23 0.2545231848684537 1106 24-25 0.2452574555812035 1106 26-27 0.13436150406439396 1106 28-29 0.2470663053536697 1106 30-31 0.3891877822821357 1106 32-33 0.29746387410791897 1106 34-35 0.26217744298987355 1106 36-37 0.08243251154403453 1106 38-39 0.29907864112343674 1106 40-41 0.19771786771275401 1106 42-43 0.35414745347224397 1106 44-45 0.3578376076861858 1106 46-47 0.36815247346091695 1106 48-49 0.6383400176293534 1106 50-51 0.5647144515622031 1106 52-53 0.2992298793838657 1106 54-55 0.12214195964595831 1106 56-57 0.4031859891013738 1106 58-59 0.48869428583137875 1106 60-61 0.3664579431190518 1106 62-63 0.4269148084299772 1106 64-65 -0.045854892612162246 1106 66-67 0.5318343713277791 1106 68-69 0.03866225197488049 1106 70-71 0.038857266948586044 1106 72-73 0.43087635507966837 1106 74-75 0.592319117295137 1106 76-77 0.5762332378039261 1106 78-79 0.6381961716645179 1106 80-81 -0.2959781358291629 1106 82-83 0.11373648346889098 1106 84-85 0.30182068868599643 1106 86-87 0.5193495064569582 1106 88-89 0.2590891077033639 1106 90-91 0.5241258944789813 1106 92-93 0.6150861379795991 1106 94-95 0.7362924643739746 1106 96-97 0.3489660961536458 1106 98-99 -0.041273974484518305 1106 100-101 -0.15382593697086122 1107 1 0.10519589361438264 1107 2 0.06720992503028356 1107 3 0.03103702257029539 1107 4 0.13802170890804177 1107 5 0.06352779287974641 1107 6 0.013865709972797902 1107 7 -0.007069639029168684 1107 8 0.03202540903063067 1107 9 0.08607221534133913 1107 10-11 0.15443285785426752 1107 12-13 0.16586048027313893 1107 14-15 0.1645280395395261 1107 16-17 0.24441176434256562 1107 18-19 0.0920906232100549 1107 20-21 0.06956804752220336 1107 22-23 0.1474599429540433 1107 24-25 0.16712616653103396 1107 26-27 0.19605302247719436 1107 28-29 0.24935435855786636 1107 30-31 0.2912264407832197 1107 32-33 0.30651778423293763 1107 34-35 0.4187898857885486 1107 36-37 0.21672482504757085 1107 38-39 0.3006545803398595 1107 40-41 0.34269343910682437 1107 42-43 0.3672875046205988 1107 44-45 0.5571335436826175 1107 46-47 0.31207968921906826 1107 48-49 0.2319102475492869 1107 50-51 0.29655941505861705 1107 52-53 0.46677481996879777 1107 54-55 0.43637386730645034 1107 56-57 0.31208029280870875 1107 58-59 0.2754803915586237 1107 60-61 0.31648379595295495 1107 62-63 0.2893552849890355 1107 64-65 0.2180994454004761 1107 66-67 0.46807462620266804 1107 68-69 0.4459604915052573 1107 70-71 0.2827826250180756 1107 72-73 0.1618334982021956 1107 74-75 0.1132359176121831 1107 76-77 0.09560970460393392 1107 78-79 0.10124287395752418 1107 80-81 0.14383197742791864 1107 82-83 0.2142196373288563 1107 84-85 0.3168137842764658 1107 86-87 0.4583066310026034 1107 88-89 0.5148572310043527 1107 90-91 0.5366985765479519 1107 92-93 0.3576801525067239 1107 94-95 0.050055305646861115 1107 96-97 -0.18843691701736276 1107 98-99 -0.035540682845976335 1107 100-101 -0.21726910098619356 1108 1 0.08108671450007421 1108 2 0.16932744220143547 1108 3 0.03243853833845378 1108 4 0.061322389538560174 1108 5 0.02181826923231256 1108 6 0.11258747431391214 1108 7 0.11105653290884732 1108 8 0.13392907295099832 1108 9 0.23136366994303614 1108 10-11 0.09246277363012467 1108 12-13 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-0.39707843331122916 2316 62-63 -0.3468782528194865 2316 64-65 -0.41003527565077746 2316 66-67 -0.019097220179286722 2316 68-69 -0.024407563848406255 2316 70-71 -0.338319505981719 2316 72-73 -0.4174241405453678 2316 74-75 -0.26970590870988076 2316 76-77 -0.1980845129150346 2316 78-79 0.026721840146436193 2316 80-81 -0.43804507580346197 2316 82-83 -0.20723426853502147 2316 84-85 -0.22138649481764006 2316 86-87 0.211405738482906 2316 88-89 0.148119579464975 2316 90-91 0.042835739672643314 2316 92-93 -0.07189146711126781 2316 94-95 0.11322142478515573 2316 96-97 0.2570307109734493 2316 98-99 0.3183157581227114 2316 100-101 0.4158675097647446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 16.0 5 39.0 6 65.0 7 193.0 8 456.0 9 784.0 10 1197.0 11 1882.0 12 2648.0 13 3881.0 14 4941.0 15 6291.0 16 7747.0 17 9925.0 18 9791.0 19 8885.0 20 9083.0 21 9891.0 22 10931.0 23 12133.0 24 14371.0 25 16736.0 26 20460.0 27 24512.0 28 29305.0 29 34794.0 30 41528.0 31 49533.0 32 59953.0 33 73060.0 34 91927.0 35 119868.0 36 156634.0 37 176797.0 38 86734.0 39 3532.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50780309397788 19.238272642602393 14.848458690170602 23.40546557324913 2 16.188923808112555 20.307291188446534 39.036217305652094 24.467567697788816 3 19.77470691181094 24.21823461957144 29.305002535160586 26.702055933457032 4 13.396623993326859 16.448514398958324 35.708853271738825 34.446008335975996 5 14.0357403030168 37.43191489555206 33.71327906230134 14.819065739129803 6 33.45212447445824 35.29196360665897 16.899115063546596 14.356796855336196 7 29.77762552804056 31.828726314842566 19.91593149125286 18.477716665864026 8 27.130812307876834 35.24516801434944 18.815190121859356 18.80882955591437 9 26.685936152639044 16.406262068038068 19.265881651675418 37.641920127647474 10-11 25.70736308200314 26.36886194028156 26.78606963365866 21.13770534405664 12-13 26.428469529708842 23.35990535477874 27.205594390344295 23.006030725168124 14-15 23.029701117006248 24.916108678447042 25.259670104704 26.79452009984271 16-17 22.923888559250035 28.09525583559209 26.04142909195652 22.939426513201354 18-19 22.927341437905884 26.754448534389308 27.98544520781332 22.332764819891487 20-21 23.591111926878934 25.7978194162636 28.02347230564155 22.587596351215915 22-23 23.27939876296079 25.827714076205034 27.92252103757186 22.970366123262316 24-25 23.14624628758585 25.996916941420523 28.031481181121286 22.82535558987234 26-27 22.767149628509532 26.306986710673623 27.932621007022696 22.993242653794148 28-29 22.74969991758524 26.508658093229542 27.88249490473235 22.859147084452868 30-31 22.836975968873208 26.14792313892112 27.90707394884833 23.108026943357345 32-33 22.66455919915023 26.15737312261081 27.810484211712343 23.36758346652661 34-35 23.225663690677685 26.278456787122355 27.695872070489546 22.800007451710417 36-37 23.25659159078663 25.862651756288557 27.682000779623657 23.19875587330116 38-39 22.924938538825607 26.22946201765302 27.64042248395376 23.205176959567613 40-41 23.209481762573372 26.021006469505544 27.798700646677943 22.97081112124314 42-43 22.973360992758938 25.963286746646386 27.621489301962963 23.441862958631713 44-45 22.965822861873043 26.149740443476833 27.636386435275334 23.248050259374793 46-47 23.111888715538225 26.378266491357355 27.184468224884167 23.32537656822025 48-49 23.081494296843978 26.377221541237823 27.145441609550296 23.395842552367903 50-51 23.14569058027443 26.501616038076165 27.14789497070051 23.204798410948897 52-53 23.334417658384908 26.344237463551686 27.098736882468554 23.222607995594853 54-55 23.04051407911272 26.373178038601363 27.174518482441655 23.411789399844256 56-57 22.969184875301103 26.60583845434613 27.016503851322682 23.408472819030084 58-59 23.063821010039696 26.271318118253824 27.069478279121622 23.59538259258485 60-61 22.87223167725398 26.437556052487388 27.37823832313674 23.31197394712189 62-63 22.922434715605466 26.43855556999303 27.351569378781697 23.287440335619806 64-65 22.712536039420968 26.407297931634822 27.250027486731405 23.630138542212805 66-67 22.702879521685006 26.579057363270426 27.04396972367859 23.67409339136598 68-69 22.93094220604391 26.1980094737636 27.260604827021577 23.610443493170912 70-71 22.77150352239696 26.283632689370297 27.061576750817107 23.88328703741564 72-73 22.813759903174013 26.328018020391973 26.91269032857775 23.945531747856265 74-75 22.75765062501647 26.60302163228478 26.79879076554863 23.84053697715012 76-77 22.75106289600202 26.26550274367555 27.108777490138852 23.874656870183575 78-79 22.894675388516998 26.358503304314006 27.011279100725016 23.73554220644398 80-81 22.758454622072087 26.579555341512894 26.796091803099937 23.865898233315082 82-83 22.75701486569985 26.536591792970594 26.959619089157854 23.7467742521717 84-85 22.70192391573009 26.3924580904055 26.889112229023393 24.01650576484101 86-87 22.690283258253654 26.391291211474808 26.884295511802613 24.034130018468918 88-89 22.601690730061545 26.408400312394797 26.760912123743957 24.228996833799698 90-91 22.52917681556872 26.406545820333427 26.931539947941303 24.13273741615655 92-93 22.516043588584463 26.22666154786271 26.922561431326624 24.3347334322262 94-95 22.371937897223205 26.10468315053513 27.031327164490214 24.492051787751453 96-97 22.548069977129224 25.929937457463716 26.9618484168097 24.56014414859736 98-99 22.269749784422245 26.069960773481167 26.799699417826485 24.860590024270103 100-101 22.359433764246532 25.85097557451812 26.98824476548139 24.80134589575396 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 182.0 1 134.5 2 131.0 3 184.0 4 159.0 5 135.0 6 233.0 7 521.0 8 797.0 9 876.5 10 824.0 11 707.5 12 643.0 13 668.5 14 774.5 15 837.0 16 839.5 17 872.0 18 933.0 19 1010.5 20 1113.5 21 1303.0 22 1554.0 23 1822.0 24 2109.0 25 2519.0 26 3221.5 27 4156.0 28 4961.5 29 5981.0 30 7454.0 31 9466.0 32 12246.0 33 14456.5 34 16417.0 35 19047.0 36 22016.5 37 25120.5 38 27674.0 39 29744.0 40 31583.0 41 33739.0 42 35955.5 43 38090.0 44 40597.5 45 42634.0 46 43527.5 47 44578.5 48 46228.5 49 46368.0 50 45114.5 51 43756.5 52 42332.0 53 41670.0 54 41600.5 55 39256.0 56 35359.0 57 32188.0 58 29684.5 59 26489.5 60 21955.0 61 17995.0 62 14573.5 63 10889.0 64 7898.5 65 6036.5 66 4504.5 67 3342.5 68 2451.5 69 1713.0 70 1269.0 71 888.0 72 706.5 73 560.5 74 386.0 75 304.5 76 226.5 77 128.5 78 79.5 79 51.0 80 25.0 81 15.5 82 10.0 83 5.5 84 3.0 85 3.5 86 1.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.451913667129777E-4 2 0.04834030118188402 3 8.177870500694664E-4 4 0.0 5 0.08977484505207031 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.543261389274814E-5 26-27 0.008995657550764131 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01022233812586833 36-37 0.0 38-39 0.0035891764975271028 40-41 0.005497346281022525 42-43 1.8173045557099256E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.086522778549628E-5 68-69 0.0016355741001389328 70-71 1.8173045557099256E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0021353328529591623 82-83 9.99517505640459E-4 84-85 0.00608797026162825 86-87 0.003043985130814125 88-89 4.543261389274814E-5 90-91 0.0038617721808835914 92-93 0.0012266805751041997 94-95 4.543261389274814E-5 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1100531.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.51155716732075 #Duplication Level Percentage of deduplicated Percentage of total 1 82.50822790765892 53.22734261439268 2 10.417093397044761 13.44045832401545 3 2.973287558685153 5.754342309510023 4 1.2771238568611887 3.2955699480659892 5 0.6945776034078595 2.2404141384693386 6 0.433745929748222 1.6788975205827068 7 0.29976287245760797 1.353671877823248 8 0.22476338538497168 1.1599868788306515 9 0.17001879096106493 0.9871359257343146 >10 0.9258102141959366 10.838023815014065 >50 0.05059597288439521 2.2337831994332626 >100 0.023298105329041287 2.70994774671156 >500 0.0012708040356546831 0.4840554908801101 >1k 4.236013452182277E-4 0.5963702105367108 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2614 0.23752170543128726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2252 0.20462849297293761 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1681 0.15274444790741923 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 2 9.086522778549628E-5 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 3 9.086522778549628E-5 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 4 9.086522778549628E-5 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 5 9.086522778549628E-5 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 6 9.086522778549628E-5 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 7 9.086522778549628E-5 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 8 9.086522778549628E-5 0.0 0.0 9.086522778549628E-5 9.086522778549628E-5 9 9.086522778549628E-5 0.0 0.0 1.8173045557099256E-4 9.086522778549628E-5 10-11 9.086522778549628E-5 0.0 0.0 1.8173045557099256E-4 9.086522778549628E-5 12-13 9.086522778549628E-5 0.0 0.0 1.8173045557099256E-4 3.18028297249237E-4 14-15 9.086522778549628E-5 0.0 0.0 1.8173045557099256E-4 4.0889352503473326E-4 16-17 9.086522778549628E-5 0.0 0.0 1.8173045557099256E-4 4.5432613892748135E-4 18-19 2.7259568335648884E-4 0.0 0.0 1.8173045557099256E-4 4.5432613892748135E-4 20-21 4.5432613892748135E-4 0.0 0.0 2.271630694637407E-4 4.5432613892748135E-4 22-23 4.5432613892748135E-4 0.0 0.0 7.269218222839702E-4 4.997587528202295E-4 24-25 5.451913667129777E-4 0.0 0.0 0.0012266805751042 5.451913667129777E-4 26-27 5.451913667129777E-4 0.0 0.0 0.0017264393279244291 5.451913667129777E-4 28-29 6.814892083912221E-4 0.0 0.0 0.003861772180883592 5.451913667129777E-4 30-31 7.269218222839702E-4 0.0 0.0 0.011903344839900012 5.451913667129777E-4 32-33 7.269218222839702E-4 0.0 0.0 0.02907687289135881 5.451913667129777E-4 34-35 7.269218222839702E-4 0.0 0.0 0.057835717485468376 5.451913667129777E-4 36-37 8.177870500694664E-4 0.0 0.0 0.10858394720366804 5.451913667129777E-4 38-39 9.086522778549627E-4 0.0 0.0 0.1830025687599895 5.451913667129777E-4 40-41 9.086522778549627E-4 0.0 0.0 0.2643269476280087 5.451913667129777E-4 42-43 9.99517505640459E-4 0.0 0.0 0.34169868908735873 5.451913667129777E-4 44-45 9.99517505640459E-4 0.0 0.0 0.44196846794865385 5.906239806057258E-4 46-47 9.99517505640459E-4 0.0 0.0 0.5626374904477929 6.36056594498474E-4 48-49 9.99517505640459E-4 0.0 0.0 0.6964365383619362 6.36056594498474E-4 50-51 9.99517505640459E-4 0.0 0.0 0.835551202101531 6.36056594498474E-4 52-53 9.99517505640459E-4 0.0 0.0 0.9740752418605201 6.36056594498474E-4 54-55 0.0010903827334259554 0.0 0.0 1.1262290657873337 6.36056594498474E-4 56-57 0.0010903827334259554 0.0 0.0 1.3055061602081177 6.36056594498474E-4 58-59 0.0010903827334259554 0.0 0.0 1.4910529553461012 6.36056594498474E-4 60-61 0.0010903827334259554 0.0 0.0 1.7061763821282634 6.36056594498474E-4 62-63 0.0011358153473187034 0.0 0.0 1.9126676122708037 6.36056594498474E-4 64-65 0.0011812479612114516 0.0 0.0 2.1311984850949224 6.36056594498474E-4 66-67 0.0011812479612114516 0.0 0.0 2.3839401161802805 6.814892083912221E-4 68-69 0.001272113188996948 0.0 0.0 2.656535799536769 7.269218222839702E-4 70-71 0.001272113188996948 0.0 0.0 2.9588444123791153 7.269218222839702E-4 72-73 0.001272113188996948 0.0 0.0 3.270421278455582 7.269218222839702E-4 74-75 0.001272113188996948 0.0 0.0 3.5803625704319098 7.269218222839702E-4 76-77 0.001272113188996948 0.0 0.0 3.924287457600013 7.269218222839702E-4 78-79 0.001272113188996948 0.0 0.0 4.305512520774062 7.269218222839702E-4 80-81 0.001272113188996948 0.0 0.0 4.717858924464645 7.269218222839702E-4 82-83 0.001272113188996948 0.0 0.0 5.134703156930609 8.177870500694664E-4 84-85 0.0014084110306751925 0.0 0.0 5.56008872080841 8.177870500694664E-4 86-87 0.0014538436445679405 0.0 0.0 6.006509584918553 8.632196639622146E-4 88-89 0.0014538436445679405 0.0 0.0 6.483006839425695 9.086522778549627E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2425 0.0 37.26878 1 GTATCAA 3760 0.0 36.687103 1 TATCAAC 4825 0.0 28.392162 2 ATCAACG 4845 0.0 28.160126 3 TCAACGC 4895 0.0 27.871216 4 CAACGCA 4985 0.0 27.272665 5 AACGCAG 5215 0.0 26.229488 6 GTACATG 5265 0.0 25.748373 1 TACATGG 5225 0.0 25.672379 2 ACATGGG 5195 0.0 25.347822 3 TAAGGTG 585 0.0 23.565077 5 GTGTAGG 495 0.0 23.062656 1 ACGCAGA 5960 0.0 22.711731 7 CGCAGAG 6015 0.0 22.583023 8 GGGCGTA 175 4.0523773E-6 21.712189 9 GTACACG 200 5.3134136E-7 21.405027 1 CATGGGA 2650 0.0 20.98779 4 CATGGGG 2540 0.0 20.960953 4 CTATTAG 300 1.6370905E-10 20.61225 1 AGGTAAG 545 0.0 20.074032 2 >>END_MODULE