FastQCFastQC Report
Thu 26 May 2016
SRR938441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103915
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27500.2491133828238587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.20218948016831007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.13388711993224117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.037.7844961
GTATCAA40600.033.7021371
ATCAACG50600.027.052193
TCAACGC51350.026.6570784
TATCAAC51600.026.5279252
CAACGCA52600.025.9332315
AACGCAG54700.025.1034366
GTACATG53650.024.6156671
TACATGG54450.024.1792262
TACCTGG10350.023.8794042
GTACCTG11000.023.4046781
ACATGGG55250.022.9688633
ACGCAGA61700.022.021387
CGCAGAG61500.021.8612488
TATAGGG2405.8844307E-921.7842182
GAGTACT29250.021.27356112-13
CATGGGG28750.020.9955084
ACCGAGT3650.020.8252758
GTACTTT30650.020.30184714-15
CAGCGTA1656.314588E-520.1547649
Produced by FastQC (version 0.11.5)