FastQCFastQC Report
Thu 26 May 2016
SRR938441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103915
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27620.2502004230398174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.21586806955245647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.15309149708084407No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26550.033.869941
GTATCAA38550.033.200531
GTACATG51000.026.5883771
TATCAAC49100.026.3575212
ATCAACG48550.026.3453753
TCAACGC48900.026.2540454
CAACGCA49050.026.0768185
TACATGG51700.025.8602622
ACATGGG51450.025.0452443
AACGCAG51250.024.9291636
ATTCGCG1153.6361598E-922.71507380-81
CGCGGAA1053.4535333E-822.6167482-83
TGTACCG1503.009023E-522.1895315
CATGGGG27100.021.9321924
GTATTAC2405.81349E-921.8071351
AGGACCG4850.021.5686175
ACGCAGA59400.021.428797
TACCTGG9550.021.4230962
CGCAGAG59300.021.3848348
GGACCGA4750.020.9978586
Produced by FastQC (version 0.11.5)