FastQCFastQC Report
Thu 26 May 2016
SRR938440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107211
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25290.22841174807692483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21990.19860713089013748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.13231443690498015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.043.7770081
GTATCAA43100.037.41371
ATCAACG52850.030.1312683
TATCAAC53050.030.1072772
TCAACGC53200.029.8436854
CAACGCA53300.029.697165
AACGCAG54950.028.709856
GTACATG51650.026.3248421
TACATGG51800.025.5113032
ACGCAGA62600.025.269297
CGCAGAG63600.024.7972818
ACATGGG53850.024.1870213
TACCTGG9850.023.6470742
GAGTACT29050.023.38157712-13
GCAGAGT66700.022.6466929
GTACTTT31950.020.96386714-15
AGTACTT31200.020.47630312-13
CAGAGTA63450.020.28763410-11
CATGGGG28350.019.9531524
AGAGTAC56400.019.8337810-11
Produced by FastQC (version 0.11.5)