##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938440_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1107211 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.84263613710485 31.0 31.0 34.0 27.0 34.0 2 31.008025570555205 31.0 31.0 34.0 27.0 34.0 3 31.061648592725327 31.0 31.0 34.0 27.0 34.0 4 34.727549672104054 37.0 35.0 37.0 32.0 37.0 5 34.71547247995188 37.0 35.0 37.0 32.0 37.0 6 34.677573651273335 37.0 35.0 37.0 30.0 37.0 7 34.57650529122272 37.0 35.0 37.0 30.0 37.0 8 34.52953321453634 37.0 35.0 37.0 30.0 37.0 9 35.965989319109006 39.0 35.0 39.0 30.0 39.0 10-11 35.77850969688704 38.0 35.0 39.0 30.0 39.0 12-13 35.58024577067967 38.0 35.0 39.0 30.0 39.0 14-15 36.68713912704986 39.0 36.0 41.0 30.0 41.0 16-17 36.612208061516725 39.0 36.0 41.0 30.0 41.0 18-19 36.59002710413824 39.0 36.0 41.0 30.0 41.0 20-21 36.50110412559124 39.0 36.0 41.0 29.5 41.0 22-23 36.33277171198624 39.0 35.5 41.0 29.0 41.0 24-25 36.176846147662914 39.0 35.0 41.0 28.0 41.0 26-27 35.947270213175265 39.0 35.0 40.0 27.0 41.0 28-29 35.69591794156669 38.0 34.5 40.0 27.0 41.0 30-31 35.418089686608965 38.0 34.0 40.0 26.5 41.0 32-33 35.12902283304628 38.0 34.0 40.0 25.5 41.0 34-35 34.826930458602746 38.0 34.0 40.0 25.0 41.0 36-37 34.6069696742536 38.0 33.5 40.0 24.5 41.0 38-39 34.358883717737626 38.0 33.0 40.0 24.0 41.0 40-41 34.05649374870734 38.0 33.0 40.0 23.5 41.0 42-43 33.6365412735242 38.0 32.5 40.0 21.0 41.0 44-45 33.16074668694585 37.0 31.5 40.0 18.5 41.0 46-47 32.66153244503532 37.0 31.0 40.0 15.0 41.0 48-49 32.173657956794145 37.0 30.5 39.5 11.0 41.0 50-51 31.45938759640213 35.5 30.0 38.5 9.0 40.0 52-53 31.479394623066426 36.0 30.0 39.0 8.0 40.0 54-55 31.426463429283125 36.0 30.0 39.0 6.5 40.0 56-57 30.983055623544203 36.0 29.0 39.0 2.0 40.0 58-59 30.5085584409837 35.0 28.0 39.0 2.0 40.0 60-61 29.934226177304957 35.0 27.5 38.5 2.0 40.0 62-63 29.18333271616702 34.0 26.0 38.0 2.0 40.0 64-65 28.48564636731391 34.0 26.0 38.0 2.0 40.0 66-67 27.767658558305506 33.5 24.0 37.0 2.0 39.5 68-69 26.901687663868948 32.5 22.0 36.5 2.0 39.0 70-71 26.040202815904106 32.0 19.0 36.0 2.0 39.0 72-73 25.131991553552126 31.0 15.0 35.0 2.0 38.0 74-75 24.170926318470464 30.5 7.5 35.0 2.0 37.0 76-77 23.213457055610903 30.0 2.0 34.0 2.0 36.5 78-79 22.221902148732266 29.0 2.0 34.0 2.0 36.0 80-81 21.256510276722324 28.5 2.0 34.0 2.0 35.5 82-83 20.253780896324187 26.5 2.0 33.5 2.0 35.0 84-85 19.278723748228657 25.5 2.0 33.0 2.0 35.0 86-87 18.29767316256793 24.5 2.0 32.0 2.0 34.5 88-89 17.229803081797417 20.0 2.0 31.5 2.0 34.0 90-91 16.09275603295126 12.5 2.0 31.0 2.0 34.0 92-93 14.953634402114863 2.0 2.0 31.0 2.0 34.0 94-95 13.72986720688288 2.0 2.0 30.0 2.0 34.0 96-97 12.360831404312275 2.0 2.0 29.0 2.0 34.0 98-99 10.746797132615193 2.0 2.0 26.5 2.0 33.5 100-101 7.653210183063572 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5802675777055661 1101 2 0.5658212522278134 1101 3 0.4424702017164286 1101 4 0.40707707686607364 1101 5 0.3505334089636065 1101 6 0.45897111218414466 1101 7 0.3489619758842579 1101 8 0.4145710580251034 1101 9 0.3719798105831771 1101 10-11 0.4870862173268904 1101 12-13 0.37866178089870317 1101 14-15 0.38471149717371844 1101 16-17 0.4659982920645831 1101 18-19 0.5308664249250015 1101 20-21 0.6402980727118077 1101 22-23 0.8259999020511515 1101 24-25 0.8883630580012465 1101 26-27 0.8691784572721772 1101 28-29 0.7926020346546778 1101 30-31 0.736291517970038 1101 32-33 0.7513790658380088 1101 34-35 0.7283365904483361 1101 36-37 0.9844666824043387 1101 38-39 0.8565813774713718 1101 40-41 0.7679264726636745 1101 42-43 0.7885787683228997 1101 44-45 0.7365905109857565 1101 46-47 0.9612049833067005 1101 48-49 0.6704012411005422 1101 50-51 0.7591464690127445 1101 52-53 0.6766464739516671 1101 54-55 0.7842006684236473 1101 56-57 0.9956752718052329 1101 58-59 1.0650694396588918 1101 60-61 1.0772840620542858 1101 62-63 0.8396606364669132 1101 64-65 0.9726906698254751 1101 66-67 1.32696412836912 1101 68-69 1.6781600465517315 1101 70-71 1.7489115275915914 1101 72-73 1.9293520197996727 1101 74-75 2.0996638617874055 1101 76-77 2.550351140184482 1101 78-79 2.587364437905542 1101 80-81 3.0455911417897923 1101 82-83 2.8076944380323425 1101 84-85 3.0451321081312024 1101 86-87 3.2747829588255186 1101 88-89 3.431833380751872 1101 90-91 3.4121421770194615 1101 92-93 3.43163935108681 1101 94-95 3.480351657138355 1101 96-97 3.2965291996708252 1101 98-99 2.491163782438637 1101 100-101 2.301880475599325 1102 1 0.8325282959116755 1102 2 0.5284926896445938 1102 3 0.5370514186230011 1102 4 0.2945260954102693 1102 5 0.4458771787694289 1102 6 0.6622311856449343 1102 7 0.7975503854454828 1102 8 0.875877206119263 1102 9 0.818373711621156 1102 10-11 0.9408239137413403 1102 12-13 0.9875360701719984 1102 14-15 1.1405457117807671 1102 16-17 1.1211330584494235 1102 18-19 1.2202777158220428 1102 20-21 1.0735947852412338 1102 22-23 0.921554172986653 1102 24-25 1.0342220761253458 1102 26-27 1.0641979366174965 1102 28-29 0.955502968530844 1102 30-31 1.0277168617581154 1102 32-33 1.2513315775137457 1102 34-35 1.288573703304337 1102 36-37 1.3503486958362245 1102 38-39 1.6819106450735575 1102 40-41 1.688150197465177 1102 42-43 1.9631600034942167 1102 44-45 2.018294446854597 1102 46-47 2.095775022192342 1102 48-49 1.9931661391924251 1102 50-51 2.2392589067528057 1102 52-53 2.2538711649579177 1102 54-55 2.273462371099246 1102 56-57 2.541868625996898 1102 58-59 2.4890511347363606 1102 60-61 2.3882627233584266 1102 62-63 2.3224196350477406 1102 64-65 2.793065626675027 1102 66-67 3.0081197567401077 1102 68-69 2.8776037025682264 1102 70-71 3.2281262533556507 1102 72-73 3.3911622747206494 1102 74-75 3.238306481097254 1102 76-77 3.386703635056655 1102 78-79 3.4024558620621193 1102 80-81 3.59595844571324 1102 82-83 3.8093432874450386 1102 84-85 4.057343913363322 1102 86-87 4.028165132073884 1102 88-89 4.2467631619585475 1102 90-91 4.419327069157038 1102 92-93 4.2792077662370485 1102 94-95 4.017650539518906 1102 96-97 3.7952442752046593 1102 98-99 3.178825311233913 1102 100-101 2.2968955714070196 1103 1 0.597496802381599 1103 2 0.5633379199572488 1103 3 0.45915033009868367 1103 4 0.5101867331672238 1103 5 0.4039108825538449 1103 6 0.4098004247155842 1103 7 0.40918898291977257 1103 8 0.6101148759093462 1103 9 0.6089910263177956 1103 10-11 0.6820853671132738 1103 12-13 0.5332872972263516 1103 14-15 0.6158048718841158 1103 16-17 0.690235933855412 1103 18-19 0.7059953739901204 1103 20-21 0.8418164125280185 1103 22-23 1.0601257053258948 1103 24-25 0.9826287214158356 1103 26-27 1.0315772287134948 1103 28-29 1.0247705753338536 1103 30-31 0.8947137694773986 1103 32-33 1.1898802809349647 1103 34-35 1.1465557826036985 1103 36-37 1.063943057093475 1103 38-39 1.1961372117080558 1103 40-41 1.3106364222885105 1103 42-43 1.4145175458574144 1103 44-45 1.7019868168075973 1103 46-47 1.6120497059599401 1103 48-49 1.380872507422879 1103 50-51 1.407130577103235 1103 52-53 1.6511994032918302 1103 54-55 1.447842877278621 1103 56-57 1.8707957187341933 1103 58-59 1.9461794643859385 1103 60-61 2.0587467483042055 1103 62-63 2.3603208273398693 1103 64-65 2.382143805342441 1103 66-67 2.688372223819769 1103 68-69 2.589194048011265 1103 70-71 2.597568190078043 1103 72-73 2.801890828508693 1103 74-75 2.7651110032983794 1103 76-77 3.0210816508086076 1103 78-79 3.466193941735046 1103 80-81 3.231531088368037 1103 82-83 3.4371917138062145 1103 84-85 3.831252016591538 1103 86-87 3.7758173853918606 1103 88-89 3.8972589126667643 1103 90-91 3.487007028480054 1103 92-93 3.5277692849827016 1103 94-95 3.329013633459903 1103 96-97 3.270616282800461 1103 98-99 2.9812021129024995 1103 100-101 2.519675977969295 1104 1 0.577386782493015 1104 2 0.551456680440932 1104 3 0.5533769626335534 1104 4 0.4255645351703521 1104 5 0.5111024067072805 1104 6 0.7058791614497721 1104 7 0.4648251094150666 1104 8 0.495871501922835 1104 9 0.6745292672324723 1104 10-11 0.771702017070929 1104 12-13 0.7436147613181987 1104 14-15 0.9330091467943262 1104 16-17 0.8761783867376352 1104 18-19 0.9502011608221252 1104 20-21 0.7622401724114738 1104 22-23 0.815397153688572 1104 24-25 0.9491831295114395 1104 26-27 0.9983169770077254 1104 28-29 1.0955656645080438 1104 30-31 1.0717688151108362 1104 32-33 0.9721132531590868 1104 34-35 1.0090329496645651 1104 36-37 1.2464528634961098 1104 38-39 1.298733758945879 1104 40-41 1.1683377333777827 1104 42-43 1.343981608514575 1104 44-45 1.0391883663652095 1104 46-47 1.1330710156216384 1104 48-49 1.2151049637766747 1104 50-51 1.4383922369078554 1104 52-53 1.6070872193435015 1104 54-55 1.747430911034062 1104 56-57 2.1081564905280885 1104 58-59 2.265855212316776 1104 60-61 2.2719167713723714 1104 62-63 2.25101472157672 1104 64-65 2.2452795992798293 1104 66-67 2.517138158210148 1104 68-69 2.787727062089008 1104 70-71 2.896702403959818 1104 72-73 3.175956827463974 1104 74-75 2.8729372263814668 1104 76-77 3.020191450073412 1104 78-79 2.888596421157011 1104 80-81 3.3043937143750384 1104 82-83 3.4047141898539337 1104 84-85 3.3030973398540446 1104 86-87 3.1540217024801436 1104 88-89 3.265615865722566 1104 90-91 3.139700585292303 1104 92-93 3.29814494563737 1104 94-95 3.3821215087555583 1104 96-97 3.356076770054573 1104 98-99 3.1391598180258384 1104 100-101 2.2758749384419508 1105 1 0.6635754605655215 1105 2 0.5608991306763542 1105 3 0.4955972165455691 1105 4 0.443452590117289 1105 5 0.4570147961840689 1105 6 0.6424011573163213 1105 7 0.6873073557749905 1105 8 0.5886573860308033 1105 9 0.6885938796048592 1105 10-11 0.7866255637587329 1105 12-13 0.7184714113578252 1105 14-15 0.7643926875771925 1105 16-17 0.9055771715650778 1105 18-19 0.9116971283760904 1105 20-21 0.9087819031777187 1105 22-23 1.011398117124621 1105 24-25 0.8256352762644994 1105 26-27 0.8122211461995263 1105 28-29 1.0027347164558975 1105 30-31 1.2942269755695506 1105 32-33 1.1671744779660074 1105 34-35 1.2111210419057983 1105 36-37 1.3769129819055053 1105 38-39 1.271301082924559 1105 40-41 1.2795683675362497 1105 42-43 1.4610328726359043 1105 44-45 1.642376251933868 1105 46-47 1.8221181462915368 1105 48-49 1.6025468856761975 1105 50-51 1.689779507120587 1105 52-53 1.7394633323452275 1105 54-55 2.0459053411305597 1105 56-57 2.0385661062414187 1105 58-59 2.1455480782282343 1105 60-61 2.068848926827439 1105 62-63 2.066875675999981 1105 64-65 2.4350019121479143 1105 66-67 2.6773832128307546 1105 68-69 2.8908038417263207 1105 70-71 3.0903771355185654 1105 72-73 2.8460203831645643 1105 74-75 2.8521266192613624 1105 76-77 3.046751594899604 1105 78-79 3.3269178669326536 1105 80-81 3.530904521951996 1105 82-83 3.4650835588033218 1105 84-85 3.846371546184752 1105 86-87 3.9729640983280454 1105 88-89 4.145504526701853 1105 90-91 3.852897716739168 1105 92-93 3.6833216273771487 1105 94-95 3.5059463498663046 1105 96-97 3.4138541852488906 1105 98-99 2.8100675457153006 1105 100-101 1.9875620392764093 1106 1 0.27136493424973196 1106 2 0.18191667453600147 1106 3 0.06937499432334704 1106 4 0.237639724620216 1106 5 0.1661843868273465 1106 6 -0.13626550935034487 1106 7 0.2400558937866819 1106 8 -0.1227227309282597 1106 9 0.29278101410778135 1106 10-11 0.08341503744294698 1106 12-13 0.28168778562682917 1106 14-15 0.34673771681696053 1106 16-17 -0.02293744831796829 1106 18-19 0.3929953739901251 1106 20-21 0.3311912599028659 1106 22-23 0.35990104110122445 1106 24-25 0.17006217684929936 1106 26-27 0.35998138011765235 1106 28-29 0.48064114920443046 1106 30-31 0.35751352527716307 1106 32-33 0.1813674604221447 1106 34-35 0.3368695799174901 1106 36-37 0.49433988249030136 1106 38-39 0.4917513753222167 1106 40-41 0.8001590108111003 1106 42-43 0.39983989017976285 1106 44-45 0.7806803455011249 1106 46-47 1.0238140527242905 1106 48-49 0.8693889909393562 1106 50-51 0.7495982205708778 1106 52-53 0.9056621627545844 1106 54-55 0.564881949317698 1106 56-57 1.2448701998086733 1106 58-59 1.3018229320294026 1106 60-61 1.4458957104531684 1106 62-63 1.5879984830175253 1106 64-65 1.5944405086391455 1106 66-67 1.7780498794974235 1106 68-69 1.9532599820771956 1106 70-71 1.7342250887349415 1106 72-73 1.7334121960300628 1106 74-75 1.9810096601970386 1106 76-77 2.3204650451920017 1106 78-79 2.9253038318449356 1106 80-81 2.7284834693204196 1106 82-83 2.6886508102653117 1106 84-85 3.0308686222081427 1106 86-87 2.4813851509596248 1106 88-89 2.516703357111208 1106 90-91 2.5347164301894587 1106 92-93 2.960859639073055 1106 94-95 2.6514434258896973 1106 96-97 2.1736553548395356 1106 98-99 2.2431728088731955 1106 100-101 1.5587199340132516 1107 1 0.18376744997300065 1107 2 0.12008934463320031 1107 3 0.04471099584802829 1107 4 0.07444323138598463 1107 5 0.02572774493674501 1107 6 -0.08715859111135416 1107 7 0.061527210729700244 1107 8 0.24651149815502293 1107 9 0.30794930074776516 1107 10-11 0.31789531188548636 1107 12-13 0.15628850985019938 1107 14-15 -0.04650451684036483 1107 16-17 0.04989121568051047 1107 18-19 0.08456884454472657 1107 20-21 0.1251070212903258 1107 22-23 0.06776115164058893 1107 24-25 0.01863202628707228 1107 26-27 0.1442687824427864 1107 28-29 0.2948008595150853 1107 30-31 0.22729549592705212 1107 32-33 0.3110484209710265 1107 34-35 0.28804244059978146 1107 36-37 0.43195947463819095 1107 38-39 0.5230351565305327 1107 40-41 0.5427887039690944 1107 42-43 0.7860697358058388 1107 44-45 0.5239431623488429 1107 46-47 0.6103053811024068 1107 48-49 0.4003381047186565 1107 50-51 0.5860232253355058 1107 52-53 0.5983646594193495 1107 54-55 0.8324653302972997 1107 56-57 1.200521810252738 1107 58-59 0.9777642318197586 1107 60-61 1.2940279765099625 1107 62-63 1.128491336076415 1107 64-65 1.043345025505925 1107 66-67 1.2757251224934194 1107 68-69 1.357022131877084 1107 70-71 1.4517234687616245 1107 72-73 1.4705366480979087 1107 74-75 1.4481912877937546 1107 76-77 1.5360815879783551 1107 78-79 1.4664381943377869 1107 80-81 1.7006767226080122 1107 82-83 2.055258395552144 1107 84-85 1.6776728913142982 1107 86-87 1.5609677695988466 1107 88-89 1.6054493071779135 1107 90-91 1.6703535561662086 1107 92-93 1.6487025969537488 1107 94-95 1.787776568637934 1107 96-97 1.6312250136922124 1107 98-99 1.167881785431229 1107 100-101 0.9111830557971272 1108 1 0.3578256208499546 1108 2 0.2595002847242647 1108 3 0.26613985220448555 1108 4 0.2785869376139374 1108 5 0.2950430062441072 1108 6 0.35890171102617785 1108 7 0.35226335041274126 1108 8 0.3176921823238601 1108 9 0.37843476087780914 1108 10-11 0.2722655357818198 1108 12-13 0.27644046189114135 1108 14-15 0.2479285621473437 1108 16-17 0.478005334996908 1108 18-19 0.4379998036689656 1108 20-21 0.5557933270863273 1108 22-23 0.4531638687128918 1108 24-25 0.5516188398245632 1108 26-27 0.6888414760940265 1108 28-29 0.6817899125857494 1108 30-31 0.7484278848194208 1108 32-33 0.6799986896580279 1108 34-35 0.6675222192678092 1108 36-37 0.9414347514456409 1108 38-39 0.8788082228674412 1108 40-41 0.8195170765180109 1108 42-43 0.8983747739006915 1108 44-45 1.0406655799049673 1108 46-47 0.9890835248542231 1108 48-49 0.9643336199537487 1108 50-51 0.7850482021138809 1108 52-53 0.8970039863057089 1108 54-55 0.8625822077156222 1108 56-57 1.0681400410785216 1108 58-59 1.347608461745164 1108 60-61 1.5184963010770183 1108 62-63 1.6088833113674674 1108 64-65 1.7107173635671593 1108 66-67 1.8429424597853554 1108 68-69 1.9670584316896012 1108 70-71 2.039875882385733 1108 72-73 2.328926777056271 1108 74-75 2.060621694157909 1108 76-77 2.176216244896686 1108 78-79 2.297195304713153 1108 80-81 2.5395321957877464 1108 82-83 2.8261941842040343 1108 84-85 2.6755637126474205 1108 86-87 2.799413943872139 1108 88-89 2.780564560507294 1108 90-91 2.624965968764581 1108 92-93 2.8195211377810647 1108 94-95 2.5448007533167907 1108 96-97 2.137598138154411 1108 98-99 1.813662288385931 1108 100-101 1.2045405320198954 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-1.3058541859255524 2315 98-99 -1.2867411872922379 2315 100-101 -0.5901181925274228 2316 1 -0.9693210961790371 2316 2 -0.8859560778844688 2316 3 -0.8909864625692308 2316 4 -0.6367650702161143 2316 5 -0.6435675726009578 2316 6 -0.6062848731124504 2316 7 -0.6576296756001341 2316 8 -0.7020938697018906 2316 9 -0.9662434211158271 2316 10-11 -1.012418987453323 2316 12-13 -0.980294694778884 2316 14-15 -1.2847901762212999 2316 16-17 -1.2395054520063056 2316 18-19 -0.8611899648757131 2316 20-21 -1.060024968773945 2316 22-23 -1.2848274051873858 2316 24-25 -1.23072528280953 2316 26-27 -1.2677217088634478 2316 28-29 -1.4210222901685512 2316 30-31 -1.098184031200482 2316 32-33 -1.4152798424824198 2316 34-35 -1.2312226284734749 2316 36-37 -1.4776840916913514 2316 38-39 -1.536744705821171 2316 40-41 -1.2247575405950784 2316 42-43 -1.1381518110650504 2316 44-45 -1.5673602261908997 2316 46-47 -1.3338438541452398 2316 48-49 -0.9457424060177679 2316 50-51 -1.1313404608491346 2316 52-53 -0.943152959421532 2316 54-55 -1.0102586684785244 2316 56-57 -1.1289850330175142 2316 58-59 -0.8738211435814769 2316 60-61 -1.2976266500816358 2316 62-63 -0.8788839512839992 2316 64-65 -0.7728236684009673 2316 66-67 -0.7343042007238765 2316 68-69 -0.37058510782598475 2316 70-71 -0.3850796599971922 2316 72-73 -0.36811661909211324 2316 74-75 -0.2333269004299794 2316 76-77 -0.3522965961173554 2316 78-79 -0.47789395515368227 2316 80-81 -0.41213617106685874 2316 82-83 -0.3833731639163389 2316 84-85 -0.2061235400786252 2316 86-87 0.026309079037080352 2316 88-89 -0.44267884666933455 2316 90-91 -0.4461613936925133 2316 92-93 -0.45789370348111724 2316 94-95 -0.4562651955948578 2316 96-97 -0.613833810674528 2316 98-99 -0.43119510260674865 2316 100-101 0.04366599210453703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4776.0 3 1966.0 4 2087.0 5 2341.0 6 2852.0 7 3418.0 8 4458.0 9 5640.0 10 6963.0 11 8554.0 12 10028.0 13 11557.0 14 13169.0 15 14789.0 16 16842.0 17 18941.0 18 19428.0 19 19845.0 20 21952.0 21 24249.0 22 26353.0 23 29066.0 24 32097.0 25 35460.0 26 38885.0 27 42180.0 28 46004.0 29 50599.0 30 55492.0 31 61324.0 32 67931.0 33 76115.0 34 83952.0 35 89400.0 36 85753.0 37 59023.0 38 13566.0 39 156.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54768559609148 18.27317010372366 14.118602717793852 23.060541582391004 2 16.009081193203016 19.684109628395625 40.12474444463517 24.182064733766193 3 19.99136274397312 24.034791156389446 28.791787017553688 27.182059082083747 4 13.147724983035747 15.710840586624307 36.09604432481371 35.04539010552624 5 14.192320483530466 37.310126811070305 33.5978845542092 14.89966815119003 6 33.60289898291606 34.81373040724023 17.246146964336546 14.337223645507164 7 30.149289063128233 31.790176720013662 19.88734878417462 18.17318543268349 8 27.47869723097885 34.59103472141282 19.142540394170123 18.787727653438214 9 27.207798309366428 16.483948377490503 19.397562712097173 36.91069060104589 10-11 25.84940650305501 26.213704075985678 26.816484645878276 21.120404775081035 12-13 26.726643436058158 23.343689836141994 27.163233722387304 22.766433005412544 14-15 23.418582044921123 24.82270592762969 25.3624929759928 26.396219051456388 16-17 23.149279875262092 28.076824748116692 26.023635882384294 22.750259494236925 18-19 23.099731006118425 26.643539065093808 28.052244890584312 22.204485038203455 20-21 23.809612007804386 25.78085648575844 28.135950636670042 22.27358086976713 22-23 23.615893938018175 25.75507679853491 27.899725746945432 22.72930351650149 24-25 23.306929592685442 25.97561543094058 28.09488720996056 22.62256776641341 26-27 22.95909793185699 26.26107933754371 27.965603230965225 22.814219499634078 28-29 22.987159888837088 26.503102514898213 27.960760504642028 22.548977091622668 30-31 23.01895316032824 26.088863691292296 27.926446107385434 22.965737040994032 32-33 22.799503105590063 26.282597402597403 27.787916431394695 23.129983060417842 34-35 23.450652245206978 26.308939901650515 27.68782936372778 22.55257848941474 36-37 23.461547322567196 25.952976709706828 27.65043012302201 22.935045844703975 38-39 22.968413654926838 26.260965159900962 27.74909239053358 23.021528794638623 40-41 23.325063715799757 26.133662492113153 27.836950782903795 22.704323009183295 42-43 23.105498442269113 26.023093620123227 27.631454150140144 23.239953787467517 44-45 23.05217694881665 26.31853731657406 27.609942655894265 23.019343078715025 46-47 23.39646145847966 26.432736229105906 27.11919756563231 23.05160474678213 48-49 23.201193844330504 26.482007421780434 27.167049679678268 23.149749054210794 50-51 23.33647822460124 26.6331040980086 27.02119482082023 23.009222856569924 52-53 23.459924758717555 26.47878927473004 27.053712159189956 23.007573807362444 54-55 23.193233590698718 26.488868375129616 27.26701628374985 23.050881750421816 56-57 23.156985781939152 26.768179941884885 27.020186737939127 23.05464753823684 58-59 23.379369956700103 26.24593329612107 27.074538324111558 23.300158423067266 60-61 23.07808338764039 26.426243338070805 27.34338905847143 23.152284215817375 62-63 23.071760493948712 26.432497785839036 27.45116990348923 23.04457181672302 64-65 23.012907739088533 26.4213258376527 27.202130921805235 23.363635501453537 66-67 22.927571875591315 26.503795475596192 27.075928027540396 23.492704621272097 68-69 23.1641469657912 26.250517097174527 27.270034015510202 23.315301921524068 70-71 23.10004394528383 26.164877465824976 27.121421316037047 23.613657272854145 72-73 23.07931674715571 26.260323831967963 26.989173836010107 23.671185584866222 74-75 22.90821340694519 26.53043896129235 26.921742837838323 23.639604793924132 76-77 23.08231461271907 26.159227962644575 27.059874985153304 23.69858243948305 78-79 23.224600471314233 26.180837255683002 26.890969969750262 23.703592303252503 80-81 23.22146753796342 26.383689425974776 26.76202515523869 23.632817880823115 82-83 23.160842811926408 26.346679842754117 26.806638507898427 23.685838837421052 84-85 23.069995858607346 26.179156555932963 26.76545491414401 23.985392671315683 86-87 23.133066921607846 26.05358551676393 26.7745590500581 24.038788511570125 88-89 23.238469043016387 26.025365395385812 26.60502971942693 24.131135842170874 90-91 23.143747778170763 26.033808392608464 26.62197486797761 24.200468961243168 92-93 23.241026567457403 25.712330777829212 26.70371583095284 24.342926823760543 94-95 23.12462114813232 25.6639110792321 26.642534574326334 24.56893319830925 96-97 23.241510514576955 25.415158443595093 26.65424255208056 24.689088489747395 98-99 23.07170490994771 25.437299415850177 26.304807515994792 25.18618815820732 100-101 24.075912828568097 24.57305477107054 26.391387032801873 24.95964536755949 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 204.0 1 149.0 2 136.0 3 184.0 4 168.5 5 153.5 6 225.0 7 436.5 8 625.5 9 752.5 10 745.5 11 638.5 12 641.5 13 676.0 14 738.5 15 807.5 16 885.0 17 971.0 18 1051.0 19 1160.5 20 1276.0 21 1434.0 22 1652.0 23 1970.0 24 2381.5 25 2861.0 26 3553.0 27 4422.0 28 5266.5 29 6244.0 30 7706.0 31 9923.5 32 12594.0 33 14726.5 34 16687.0 35 19156.5 36 22073.5 37 24984.5 38 27435.5 39 29332.5 40 31121.0 41 33456.0 42 35724.0 43 37962.5 44 40000.0 45 41592.0 46 42892.0 47 43903.0 48 45128.5 49 45500.0 50 44519.5 51 43299.5 52 42056.5 53 41271.5 54 40756.0 55 38656.0 56 35527.5 57 32767.0 58 30108.5 59 26751.5 60 22609.0 61 18847.5 62 15522.5 63 12235.0 64 9355.5 65 7237.5 66 5553.0 67 4137.5 68 3065.0 69 2253.5 70 1740.0 71 1292.0 72 913.5 73 716.0 74 568.5 75 415.0 76 295.5 77 202.5 78 133.5 79 82.0 80 45.5 81 23.0 82 13.0 83 10.0 84 5.5 85 4.5 86 3.5 87 2.5 88 1.5 89 2.0 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37788641911975224 2 0.027817642707668185 3 0.034139834232138226 4 0.042629634279283714 5 0.06168652587447199 6 0.05563528541533637 7 0.03901695340815797 8 0.03883631936460169 9 0.036759027863704385 10-11 0.03824925872304376 12-13 0.030527153361012492 14-15 0.026462887380996034 16-17 0.022579255444535866 18-19 0.02786280121855726 20-21 0.03590101615681202 22-23 0.03490752891725245 24-25 0.04393923109506679 26-27 0.0380234661685984 28-29 0.03314634699257865 30-31 0.036442918287480884 32-33 0.030346519317456203 34-35 0.030888421448125064 36-37 0.03400435869947101 38-39 0.01643769796362211 40-41 0.013231443690498017 42-43 0.013728187310277806 44-45 0.011063835167822575 46-47 0.020727756498083925 48-49 0.017973087333850547 50-51 0.01124446921137886 52-53 0.009573604308483207 54-55 0.008580117068923629 56-57 0.008851068134258058 58-59 0.004425534067129029 60-61 0.007270520253140548 62-63 0.011967005385604008 64-65 0.007631788340253123 66-67 0.0033868883166803796 68-69 0.0062318745026918995 70-71 0.013773345821166877 72-73 0.01124446921137886 74-75 0.012915334114274514 76-77 0.0056448138611339665 78-79 0.008489800047145486 80-81 0.011334786233157004 82-83 0.013999138375612237 84-85 0.008625275579812701 86-87 0.0024837180988989452 88-89 0.017250551159625403 90-91 0.026778996957219536 92-93 0.011154152189600718 94-95 0.022398621400979576 96-97 0.020231012878304137 98-99 0.011921846874714937 100-101 0.12554066027161942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1107211.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.55479539203017 #Duplication Level Percentage of deduplicated Percentage of total 1 84.35868671913019 56.98833820851213 2 9.390251045076695 12.687129760599072 3 2.6599488332128574 5.39076897542892 4 1.1259429382982542 3.0425137927935926 5 0.6259785244620811 2.1143925569920423 6 0.39014953115573237 1.58138830497132 7 0.2655851859603505 1.2559087027694034 8 0.17637041092734287 0.9531733618723945 9 0.15349547214285691 0.9332419692792391 >10 0.7891743135391048 9.60871661141321 >50 0.04358684076129353 2.043143042116055 >100 0.019217528294040657 2.367690692131108 >500 0.0012094927794341016 0.47135881929406925 >1k 4.031642598113672E-4 0.5622352018274597 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2529 0.22841174807692483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2199 0.19860713089013748 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1465 0.13231443690498015 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.031702177814347E-5 0.0 0.0 0.0 0.0 8 9.031702177814347E-5 0.0 0.0 0.0 0.0 9 9.031702177814347E-5 0.0 0.0 0.0 0.0 10-11 9.031702177814347E-5 0.0 0.0 9.031702177814347E-5 0.0 12-13 1.8063404355628693E-4 0.0 0.0 9.031702177814347E-5 0.0 14-15 1.8063404355628693E-4 0.0 0.0 1.8063404355628693E-4 4.515851088907173E-5 16-17 2.2579255444535865E-4 0.0 0.0 2.7095106533443037E-4 9.031702177814347E-5 18-19 2.7095106533443037E-4 0.0 0.0 2.7095106533443037E-4 9.031702177814347E-5 20-21 2.7095106533443037E-4 0.0 0.0 2.7095106533443037E-4 1.8063404355628693E-4 22-23 2.7095106533443037E-4 0.0 0.0 4.96743619779789E-4 1.8063404355628693E-4 24-25 4.515851088907173E-4 0.0 0.0 8.580117068923629E-4 1.8063404355628693E-4 26-27 4.515851088907173E-4 0.0 0.0 0.001535389370228439 1.8063404355628693E-4 28-29 4.515851088907173E-4 0.0 0.0 0.0037933149146820253 1.8063404355628693E-4 30-31 4.515851088907173E-4 0.0 0.0 0.012328273472716582 1.8063404355628693E-4 32-33 4.515851088907173E-4 0.0 0.0 0.029127239523451268 1.8063404355628693E-4 34-35 5.419021306688607E-4 0.0 0.0 0.05834479606868068 1.8063404355628693E-4 36-37 6.322191524470042E-4 0.0 0.0 0.10458711121909013 1.8063404355628693E-4 38-39 6.322191524470042E-4 0.0 0.0 0.1777890573702754 2.7095106533443037E-4 40-41 6.322191524470042E-4 0.0 0.0 0.2522554418263547 2.7095106533443037E-4 42-43 6.77377663336076E-4 0.0 0.0 0.3295216539575564 2.7095106533443037E-4 44-45 7.225361742251477E-4 0.0 0.0 0.43279916836086346 2.7095106533443037E-4 46-47 8.580117068923629E-4 0.0 0.0 0.5514305764664549 2.7095106533443037E-4 48-49 9.93487239559578E-4 0.0 0.0 0.6808548686745344 2.7095106533443037E-4 50-51 9.93487239559578E-4 0.0 0.0 0.8170529375159747 2.7095106533443037E-4 52-53 9.93487239559578E-4 0.0 0.0 0.9511285563456288 2.7095106533443037E-4 54-55 9.93487239559578E-4 0.0 0.0 1.0996548986597858 2.7095106533443037E-4 56-57 9.93487239559578E-4 0.0 0.0 1.2709411304620348 2.7095106533443037E-4 58-59 9.93487239559578E-4 0.0 0.0 1.4616003634356955 2.7095106533443037E-4 60-61 9.93487239559578E-4 0.0 0.0 1.6657168326543 2.7095106533443037E-4 62-63 9.93487239559578E-4 0.0 0.0 1.8670334741977817 2.7095106533443037E-4 64-65 9.93487239559578E-4 0.0 0.0 2.0840652775306605 2.7095106533443037E-4 66-67 9.93487239559578E-4 0.0 0.0 2.336501353400571 2.7095106533443037E-4 68-69 9.93487239559578E-4 0.0 0.0 2.6205483868928328 2.7095106533443037E-4 70-71 9.93487239559578E-4 0.0 0.0 2.9214395449467174 2.7095106533443037E-4 72-73 9.93487239559578E-4 0.0 0.0 3.2212468987392646 2.7095106533443037E-4 74-75 9.93487239559578E-4 0.0 0.0 3.5215961546624808 2.7095106533443037E-4 76-77 9.93487239559578E-4 0.0 0.0 3.846421323487574 2.7095106533443037E-4 78-79 9.93487239559578E-4 0.0 0.0 4.2211466468450904 2.7095106533443037E-4 80-81 9.93487239559578E-4 0.0 0.0 4.614567593710683 3.1610957622350215E-4 82-83 9.93487239559578E-4 0.0 0.0 5.020136180005437 4.515851088907173E-4 84-85 9.93487239559578E-4 0.0 0.0 5.416582747100598 4.515851088907173E-4 86-87 9.93487239559578E-4 0.0 0.0 5.8361956302818525 4.515851088907173E-4 88-89 9.93487239559578E-4 0.0 0.0 6.269220591197161 4.96743619779789E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2670 0.0 43.777008 1 GTATCAA 4310 0.0 37.4137 1 ATCAACG 5285 0.0 30.131268 3 TATCAAC 5305 0.0 30.107277 2 TCAACGC 5320 0.0 29.843685 4 CAACGCA 5330 0.0 29.69716 5 AACGCAG 5495 0.0 28.70985 6 GTACATG 5165 0.0 26.324842 1 TACATGG 5180 0.0 25.511303 2 ACGCAGA 6260 0.0 25.26929 7 CGCAGAG 6360 0.0 24.797281 8 ACATGGG 5385 0.0 24.187021 3 TACCTGG 985 0.0 23.647074 2 GAGTACT 2905 0.0 23.381577 12-13 GCAGAGT 6670 0.0 22.646692 9 GTACTTT 3195 0.0 20.963867 14-15 AGTACTT 3120 0.0 20.476303 12-13 CAGAGTA 6345 0.0 20.287634 10-11 CATGGGG 2835 0.0 19.953152 4 AGAGTAC 5640 0.0 19.83378 10-11 >>END_MODULE