FastQCFastQC Report
Thu 26 May 2016
SRR938440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107211
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25570.23094062468671284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23540.21260626926574971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.14667484336770498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.040.5857281
GTATCAA38200.036.5691381
TACCTGG10600.027.7842622
TATCAAC50450.027.399642
ATCAACG50500.027.3700393
TCAACGC50700.027.262074
CAACGCA51250.026.9695015
AACGCAG52950.026.1036266
GTACCTG12600.024.1346041
TACATGG49900.023.9890462
GTACATG51100.023.803991
ACGCAGA59350.023.2887447
CGCAGAG60000.023.036458
ACATGGG51200.022.9139943
TAACGCA1100.00287806221.5899244
GAGTACT31250.021.4310212-13
AGTACTT32650.021.16672112-13
GTACTTT32150.020.5356114-15
GCAGAGT67800.020.035969
AGAGTAC58650.019.6794110-11
Produced by FastQC (version 0.11.5)