##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938440_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1107211 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7734840062102 31.0 31.0 34.0 30.0 34.0 2 32.0277228098348 31.0 31.0 34.0 30.0 34.0 3 32.08708909141979 33.0 31.0 34.0 30.0 34.0 4 35.74659120980554 37.0 35.0 37.0 35.0 37.0 5 35.64111899177302 37.0 35.0 37.0 33.0 37.0 6 35.672753431821036 37.0 35.0 37.0 33.0 37.0 7 35.58588200442373 37.0 35.0 37.0 33.0 37.0 8 35.55638085243012 37.0 35.0 37.0 33.0 37.0 9 37.09619304721503 39.0 37.0 39.0 33.0 39.0 10-11 37.0181582372285 39.0 37.0 39.0 33.0 39.0 12-13 36.89444469030745 39.0 37.0 39.0 33.0 39.0 14-15 37.90145916180385 40.0 37.0 41.0 33.0 41.0 16-17 37.992446335883585 40.0 37.0 41.0 33.0 41.0 18-19 38.11870637123367 40.0 37.0 41.0 33.0 41.0 20-21 38.06257389061344 40.0 37.0 41.0 33.0 41.0 22-23 37.93973506404832 40.0 37.0 41.0 33.0 41.0 24-25 37.818219381852245 40.0 37.0 41.0 32.0 41.0 26-27 37.60337189569107 40.0 37.0 41.0 32.0 41.0 28-29 37.466364134749384 39.0 36.5 41.0 31.5 41.0 30-31 37.21543093412186 39.0 36.0 41.0 31.0 41.0 32-33 37.145397309094655 39.0 36.0 41.0 31.0 41.0 34-35 37.028727135116974 39.0 36.0 41.0 31.0 41.0 36-37 36.91708716766723 39.0 36.0 41.0 30.0 41.0 38-39 36.7194762335273 39.0 35.0 40.0 30.0 41.0 40-41 36.49943777653943 39.0 35.0 40.0 30.0 41.0 42-43 36.252893983170324 38.5 35.0 40.0 30.0 41.0 44-45 35.97474419961507 38.0 35.0 40.0 29.0 41.0 46-47 35.58202727393424 38.0 34.5 40.0 27.5 41.0 48-49 35.185503034200345 38.0 34.0 40.0 27.0 41.0 50-51 34.79048076653862 38.0 33.5 40.0 26.0 41.0 52-53 34.1788769258976 37.5 33.0 40.0 24.0 41.0 54-55 33.73914908721102 37.0 32.5 40.0 23.0 41.0 56-57 33.33969180219488 37.0 32.0 39.0 22.5 40.5 58-59 32.785985236779624 36.0 31.0 39.0 21.0 40.0 60-61 32.270728433875746 35.5 31.0 39.0 19.0 40.0 62-63 31.730937012005842 35.0 30.0 38.5 17.0 40.0 64-65 31.052809717389007 34.5 29.0 38.0 11.5 40.0 66-67 30.383253959724026 34.0 28.5 37.5 8.0 40.0 68-69 29.635084008377806 34.0 27.5 37.0 6.5 39.0 70-71 28.839376595788877 33.5 26.0 36.0 2.0 39.0 72-73 28.243683001704284 33.0 26.0 36.0 2.0 38.5 74-75 27.5097370781179 32.0 25.0 35.0 2.0 37.5 76-77 26.372783958974395 30.5 24.0 34.0 2.0 36.0 78-79 26.872480042196113 32.0 25.0 35.0 2.0 36.0 80-81 26.47971163581287 32.0 24.0 35.0 2.0 36.0 82-83 25.78565693440546 31.5 22.0 34.0 2.0 36.0 84-85 24.998037411116762 31.0 19.0 34.0 2.0 35.0 86-87 24.08375955441194 30.5 14.5 34.0 2.0 35.0 88-89 23.108622927337247 29.5 6.5 34.0 2.0 35.0 90-91 22.24427367502671 29.0 2.0 34.0 2.0 35.0 92-93 21.15032952165396 29.0 2.0 33.5 2.0 35.0 94-95 19.948316987457673 26.5 2.0 33.0 2.0 34.5 96-97 18.553250464455285 25.0 2.0 32.5 2.0 34.0 98-99 16.786748415613644 12.5 2.0 32.0 2.0 34.0 100-101 12.61315232597942 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1975574621135543 1101 2 0.21318185297638337 1101 3 0.19040184476850897 1101 4 0.08735155346518297 1101 5 0.07022228855033319 1101 6 0.10310321058921801 1101 7 0.09840253840084046 1101 8 0.09466106264446239 1101 9 0.2825028932602578 1101 10-11 0.32452733098324416 1101 12-13 0.22089950116871648 1101 14-15 0.37773069072229504 1101 16-17 0.2205224625414104 1101 18-19 0.260451985991196 1101 20-21 0.3205766210291614 1101 22-23 0.24668308992119847 1101 24-25 0.15111082388407482 1101 26-27 0.3184566307561312 1101 28-29 0.17300549871677617 1101 30-31 0.37880743793758853 1101 32-33 0.2878118728593648 1101 34-35 0.2801946219664302 1101 36-37 0.22044165939043125 1101 38-39 0.347805773043504 1101 40-41 0.07493474000347788 1101 42-43 0.3298352202453998 1101 44-45 0.5635342525886031 1101 46-47 0.5963326097514141 1101 48-49 0.6256144788051259 1101 50-51 0.5911524093127696 1101 52-53 0.8016529719397809 1101 54-55 0.7472390845072212 1101 56-57 0.9729833520947935 1101 58-59 0.8906451670606401 1101 60-61 0.8041986297202186 1101 62-63 0.9795002886719715 1101 64-65 1.136792071808788 1101 66-67 1.0629704505921502 1101 68-69 1.0894615669096979 1101 70-71 1.2590376613407877 1101 72-73 1.4620223774153907 1101 74-75 1.645179031950704 1101 76-77 1.6003146548991154 1101 78-79 1.5649071053598504 1101 80-81 1.4909059853304178 1101 82-83 1.6614011309215932 1101 84-85 1.7086366697575421 1101 86-87 1.447656745032429 1101 88-89 1.2731020195123328 1101 90-91 1.8130483002385311 1101 92-93 2.176730275446655 1101 94-95 1.928670966628811 1101 96-97 1.8174338717247451 1101 98-99 2.014417344960261 1101 100-101 1.9816955149255087 1102 1 0.4246429758002357 1102 2 0.30542883609793847 1102 3 0.36211414588832014 1102 4 0.2650501231533866 1102 5 0.3554992642016117 1102 6 0.3708204651065472 1102 7 0.29380420743279245 1102 8 0.32642508950824123 1102 9 0.526370963389553 1102 10-11 0.40796112038713517 1102 12-13 0.5637460243841161 1102 14-15 0.724455731498324 1102 16-17 0.5195486779228702 1102 18-19 0.5610571881544644 1102 20-21 0.6302096276063054 1102 22-23 0.5752675908280409 1102 24-25 0.5161038010915107 1102 26-27 0.7671176426592652 1102 28-29 0.6245788234291538 1102 30-31 0.7408365044449923 1102 32-33 0.6701787274702085 1102 34-35 0.8452107040700909 1102 36-37 0.8805042526547595 1102 38-39 0.8090836107905162 1102 40-41 0.9707713838209528 1102 42-43 0.9857699822467083 1102 44-45 0.9296975430180581 1102 46-47 0.9136011909281336 1102 48-49 0.9123667888381348 1102 50-51 1.0340782928239065 1102 52-53 1.0371914073456097 1102 54-55 1.0043591368174418 1102 56-57 1.1875164231464552 1102 58-59 1.3206336785391173 1102 60-61 1.2622627864462999 1102 62-63 1.3485179013608501 1102 64-65 1.3589191651763066 1102 66-67 1.5214396279299933 1102 68-69 1.841406305112045 1102 70-71 1.9099190263744 1102 72-73 1.9162116695288596 1102 74-75 1.6948741934384053 1102 76-77 1.637407602335017 1102 78-79 2.056551900000244 1102 80-81 2.1334010949462545 1102 82-83 2.1457509883652968 1102 84-85 2.1732099232374296 1102 86-87 2.054620542491076 1102 88-89 1.8347756541773492 1102 90-91 2.024699195302123 1102 92-93 2.2978401141142015 1102 94-95 2.161873798394115 1102 96-97 2.203934950257647 1102 98-99 2.6643653817746724 1102 100-101 2.3818303087074604 1103 1 0.2654346204162401 1103 2 0.3765149737563078 1103 3 0.28050854657733737 1103 4 0.15850911930360212 1103 5 0.33532420790757556 1103 6 0.19082222915930203 1103 7 0.23796156093858656 1103 8 0.20233176074069092 1103 9 0.346966798149694 1103 10-11 0.3693504156999481 1103 12-13 0.46422620992095887 1103 14-15 0.5683733813649852 1103 16-17 0.5448187336461885 1103 18-19 0.399271499810709 1103 20-21 0.4512827942885309 1103 22-23 0.41685954467211417 1103 24-25 0.5003635215410327 1103 26-27 0.6282425894782548 1103 28-29 0.5312669181484111 1103 30-31 0.3393770102092901 1103 32-33 0.5348901491929681 1103 34-35 0.41535644595803944 1103 36-37 0.6099971393714441 1103 38-39 0.5537071411789185 1103 40-41 0.6744247889616162 1103 42-43 0.542154815543725 1103 44-45 0.6048705758398185 1103 46-47 0.8703352029029432 1103 48-49 0.8226621120229751 1103 50-51 0.7775953989472484 1103 52-53 1.0090794107491945 1103 54-55 1.192910434433891 1103 56-57 1.4270877583268557 1103 58-59 1.4343541176844852 1103 60-61 1.2346644405051777 1103 62-63 1.1744746405612148 1103 64-65 1.358495014256409 1103 66-67 1.1258676597659552 1103 68-69 1.19991760023807 1103 70-71 1.4751708189633028 1103 72-73 1.481883721744822 1103 74-75 1.687463874767463 1103 76-77 1.4803264870386066 1103 78-79 1.8409989213452818 1103 80-81 2.128500660159137 1103 82-83 2.2651643889401747 1103 84-85 2.499260053466031 1103 86-87 2.2753281870655755 1103 88-89 2.005871909197115 1103 90-91 2.2060710576229923 1103 92-93 2.766356960818019 1103 94-95 2.3937546418188695 1103 96-97 2.0294364081953624 1103 98-99 1.7935238767263684 1103 100-101 1.4955079436315533 1104 1 0.18589893614185016 1104 2 0.15296954959087117 1104 3 0.19111454819307738 1104 4 0.22189060053273835 1104 5 -0.0041695578483711415 1104 6 -0.01651789159977568 1104 7 0.04272087012659398 1104 8 0.08143236194050019 1104 9 0.03092972037656949 1104 10-11 0.133344209033865 1104 12-13 0.1534796417563271 1104 14-15 0.29056586263629924 1104 16-17 0.22826497444406613 1104 18-19 0.3329014585735237 1104 20-21 0.32945628893678247 1104 22-23 0.2638573579011094 1104 24-25 0.24934354211095666 1104 26-27 0.30047757327213986 1104 28-29 0.3201457762256723 1104 30-31 0.38551901405723044 1104 32-33 0.580478565795552 1104 34-35 0.40998888024946467 1104 36-37 0.4575753067016066 1104 38-39 0.3946386115390865 1104 40-41 0.36388586670879164 1104 42-43 0.6519013577526209 1104 44-45 0.5952564078996971 1104 46-47 0.6211795755365088 1104 48-49 0.8128143424320697 1104 50-51 0.6962209127897694 1104 52-53 0.6986005111427502 1104 54-55 0.9882510548409442 1104 56-57 0.9967424172197781 1104 58-59 1.2505741023057126 1104 60-61 0.9837750508868268 1104 62-63 0.9792351664244876 1104 64-65 1.2025882180706056 1104 66-67 1.033450223349405 1104 68-69 1.2184925815012697 1104 70-71 1.2652172797360741 1104 72-73 1.6367332271257702 1104 74-75 1.593082724473998 1104 76-77 1.9226082236073196 1104 78-79 1.8551435824119977 1104 80-81 2.1293726107653654 1104 82-83 2.057256486390994 1104 84-85 2.384841883799858 1104 86-87 2.233080760739316 1104 88-89 2.240849619706271 1104 90-91 2.503416353307525 1104 92-93 2.9173593390126022 1104 94-95 2.5524573577625063 1104 96-97 2.628025227111909 1104 98-99 2.5889265475719085 1104 100-101 2.2803867789975456 1105 1 0.1318121025831971 1105 2 0.18357107554925278 1105 3 0.0690472009054659 1105 4 0.06869805317674604 1105 5 0.10367778415588447 1105 6 0.022728918960730482 1105 7 0.18842425613812708 1105 8 0.13306243357662595 1105 9 0.2515262737618045 1105 10-11 0.2336072115356913 1105 12-13 0.27712383860806256 1105 14-15 0.43965254080203664 1105 16-17 0.36165891101268244 1105 18-19 0.35215852506615164 1105 20-21 0.3414553410400316 1105 22-23 0.22534710973862815 1105 24-25 0.2737922857592707 1105 26-27 0.3916318318148697 1105 28-29 0.4628796037084655 1105 30-31 0.5730429057172941 1105 32-33 0.6400472727711346 1105 34-35 0.606468842333598 1105 36-37 0.5855176714709245 1105 38-39 0.5868332256207935 1105 40-41 0.6812591826381151 1105 42-43 0.6244422649364338 1105 44-45 0.8247375509699495 1105 46-47 0.8003911119062224 1105 48-49 0.8205125072417943 1105 50-51 0.730159501511352 1105 52-53 0.8488856571343888 1105 54-55 0.8636690617714606 1105 56-57 0.950077926053865 1105 58-59 1.131274302762563 1105 60-61 0.929989291093186 1105 62-63 1.1504723270852182 1105 64-65 1.2796922380852074 1105 66-67 1.1696019956055537 1105 68-69 1.4141213789155316 1105 70-71 1.3737056174981035 1105 72-73 1.6019193878331208 1105 74-75 1.7444765988854485 1105 76-77 1.5226245398629743 1105 78-79 1.5286121852743335 1105 80-81 1.6057707584818672 1105 82-83 1.9206540746930152 1105 84-85 2.0373456520779456 1105 86-87 1.8896958369595431 1105 88-89 1.6503340260276538 1105 90-91 1.8394574081146047 1105 92-93 1.6609781291233965 1105 94-95 1.5817920430141648 1105 96-97 2.158566926799569 1105 98-99 1.9047249563485025 1105 100-101 1.916931695318464 1106 1 -0.05243351145188768 1106 2 0.06298628022761932 1106 3 0.02413492020370711 1106 4 0.01910740990189197 1106 5 0.05766608824945507 1106 6 -0.017727221390146042 1106 7 -0.03416053918351025 1106 8 -0.06825920385027473 1106 9 -0.27435676717387025 1106 10-11 0.025004872354408292 1106 12-13 -0.02545510876036161 1106 14-15 -0.13944102644942546 1106 16-17 -0.018504831677375932 1106 18-19 0.10031057184977499 1106 20-21 0.04285300185873808 1106 22-23 0.20241143722400778 1106 24-25 0.06776889394640762 1106 26-27 -0.17537401613834191 1106 28-29 0.08209597797746682 1106 30-31 0.15169202852431596 1106 32-33 0.20264961195242392 1106 34-35 0.0991647487663414 1106 36-37 2.48665622969213E-4 1106 38-39 -0.011576131104355625 1106 40-41 0.08301941655624745 1106 42-43 0.18323173862064834 1106 44-45 0.12487790184714243 1106 46-47 -0.23039251382478199 1106 48-49 -0.06133544597458496 1106 50-51 0.13127061262246542 1106 52-53 0.0212833179530989 1106 54-55 -0.061155499632043586 1106 56-57 -0.06384020260110645 1106 58-59 0.27962517995555203 1106 60-61 0.5126851975259328 1106 62-63 0.38664191772849676 1106 64-65 0.755873524634918 1106 66-67 0.6312803581786497 1106 68-69 0.4191740104944799 1106 70-71 0.2908167286092116 1106 72-73 0.7370363468974475 1106 74-75 0.7133771836807732 1106 76-77 0.6831274638395826 1106 78-79 0.967869495215858 1106 80-81 0.8745543842128605 1106 82-83 1.2940166477924322 1106 84-85 1.4852930204990002 1106 86-87 1.4008654276028132 1106 88-89 1.7127082950335044 1106 90-91 1.9590246595765954 1106 92-93 1.7069430414040987 1106 94-95 1.5951429202071488 1106 96-97 2.0669528917118463 1106 98-99 1.6435495177520068 1106 100-101 1.5189609350845448 1107 1 -0.04467822271546851 1107 2 0.00536097052683715 1107 3 -0.004955988887196838 1107 4 0.03939709924818402 1107 5 -0.06225462835050877 1107 6 -0.11807484860570128 1107 7 -0.12812204099407154 1107 8 -0.004349922356084335 1107 9 0.11745417241065326 1107 10-11 0.03238470845122521 1107 12-13 0.1180291679691976 1107 14-15 0.04839964441011091 1107 16-17 0.052298675088394475 1107 18-19 -0.139633777302123 1107 20-21 0.0768537641991216 1107 22-23 0.03866148487028198 1107 24-25 0.1394689130049116 1107 26-27 0.015812566670881267 1107 28-29 -0.14901742015479869 1107 30-31 0.03336384098857792 1107 32-33 0.1549061394919775 1107 34-35 0.20556230927711283 1107 36-37 0.13429516838645128 1107 38-39 0.05398437966370295 1107 40-41 0.013020941237009254 1107 42-43 0.11060052078187965 1107 44-45 -0.09813867905623397 1107 46-47 0.12111272726536981 1107 48-49 0.24355725461622768 1107 50-51 0.08784961014485759 1107 52-53 0.138529363312351 1107 54-55 0.03414467189453774 1107 56-57 -0.01220879417724774 1107 58-59 -0.0386092397128337 1107 60-61 0.32985462767650375 1107 62-63 0.5545340465640152 1107 64-65 0.5355640144386165 1107 66-67 0.43917934807764425 1107 68-69 0.7800568006602902 1107 70-71 0.46953180389032667 1107 72-73 0.7174327638976372 1107 74-75 0.7602660725696602 1107 76-77 0.8321036407025524 1107 78-79 1.165560366189748 1107 80-81 1.030274986461766 1107 82-83 1.1995931677085743 1107 84-85 1.3480111451416548 1107 86-87 1.335881627335997 1107 88-89 1.4377584189138126 1107 90-91 1.5258872477221992 1107 92-93 1.7230150825704804 1107 94-95 1.440796436502172 1107 96-97 1.7096367110371773 1107 98-99 1.7202127538869405 1107 100-101 1.9790040073162949 1108 1 -0.034761307686661524 1108 2 0.04880392511503118 1108 3 0.07357013615055052 1108 4 0.061971935557117774 1108 5 0.0829602927529578 1108 6 0.15001807207607243 1108 7 0.04435478012250371 1108 8 0.04015866252107969 1108 9 0.12697361304022792 1108 10-11 0.09132417837138718 1108 12-13 0.08685164649988053 1108 14-15 0.28191371699834633 1108 16-17 0.0782862351369431 1108 18-19 -0.07400135136911956 1108 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3208.0 13 4512.0 14 6113.0 15 8115.0 16 10190.0 17 12792.0 18 13330.0 19 13066.0 20 14115.0 21 15453.0 22 17099.0 23 18878.0 24 21132.0 25 24545.0 26 27974.0 27 32612.0 28 38362.0 29 44808.0 30 51396.0 31 59591.0 32 70111.0 33 83086.0 34 99426.0 35 119040.0 36 136040.0 37 118503.0 38 37751.0 39 721.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54120937442693 19.21246213760277 14.80339557837663 23.442932909593676 2 16.169227253471448 20.27580089657421 39.11749855252854 24.437473297425804 3 19.866057921143067 24.04743518531063 29.360862359385653 26.725644534160647 4 13.429508919257485 16.549329802539894 35.71315675151349 34.30800452668913 5 14.072488507148599 37.402570424761336 33.66660344469432 14.858337623395743 6 33.40962111106194 35.30293683859716 16.897140653407526 14.390301396933374 7 29.839389240171926 31.82076406394084 19.874983178454695 18.46486351743254 8 27.12427893147738 35.08120855013181 18.95230448396918 18.842208034421624 9 26.658110618681746 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27.836892804033198 23.33774833240834 34-35 23.23155910399416 26.22946081216449 27.67911580890821 22.859864274933138 36-37 23.230435679897184 25.92236004228683 27.719414866593723 23.127789411222267 38-39 22.845569103432826 26.241044422554666 27.701216237760956 23.212170236251552 40-41 23.22619136872475 25.96324341706509 27.839597784054625 22.970967430155536 42-43 22.96108527152788 25.939494278786597 27.701990197366843 23.397430252318678 44-45 22.97169266006987 26.150917084837122 27.637680138438807 23.2397101166542 46-47 23.15563443249248 26.379187326704056 27.15641115958129 23.30876708122217 48-49 23.075592637717655 26.444101440466177 27.135116974090757 23.34518894772541 50-51 23.15601994561109 26.422967257370093 27.167585943419997 23.253426853598818 52-53 23.35941387865547 26.357939001689836 27.05902488324267 23.22362223641203 54-55 23.020408937411208 26.426534779730332 27.17494678069492 23.378109502163543 56-57 23.029395481078133 26.60143369240371 26.9985576371622 23.370613189355957 58-59 23.042085022637963 26.228966294590645 27.10192546858729 23.627023214184106 60-61 22.924447101771932 26.411858263691386 27.286217351525586 23.377477283011096 62-63 22.956599961524947 26.42504454887099 27.295429687746957 23.322925801857096 64-65 22.770973889325422 26.457176145446663 27.123038989893516 23.6488109753344 66-67 22.74222027537809 26.55226448579983 26.9755826608442 23.72993257797788 68-69 22.96354267098447 26.2348621317677 27.16509279952962 23.636502397718203 70-71 22.809032248158772 26.317850128769553 26.96411386994614 23.909003753125535 72-73 22.88306392433216 26.31372168270175 26.858335045598952 23.944879347367138 74-75 22.752935077415234 26.605633433916392 26.720561844129076 23.920869644539298 76-77 22.67684298656715 26.354552113373153 27.066611513072036 23.901993386987666 78-79 22.87088910785749 26.392711055074418 26.959495525243156 23.776904311824936 80-81 22.823649374237384 26.530402119202428 26.805372788696623 23.840575717863565 82-83 22.819464784801653 26.524217180372645 26.859883878199003 23.7964341566267 84-85 22.66318684073022 26.430268524360212 26.826016681667735 24.080527953241834 86-87 22.57295770144572 26.354415848398453 26.911017368225437 24.161609081930386 88-89 22.684790884483625 26.443740172379066 26.58431861677675 24.287150326360557 90-91 22.543620424072213 26.40550183865783 26.84815255869909 24.202725178570862 92-93 22.569276824151594 26.14882589652427 26.819884095694885 24.46201318362925 94-95 22.415305136333668 26.106203881829103 26.807877457754177 24.670613524083056 96-97 22.505205649693533 25.95653671998233 26.774989940937154 24.76326768938698 98-99 22.23812805327982 26.057680062788393 26.621484071238456 25.082707812693332 100-101 22.585949103625733 25.696513896860623 26.731366799718753 24.98617019979489 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 130.5 2 134.0 3 180.5 4 144.5 5 133.0 6 218.0 7 479.0 8 752.0 9 886.5 10 827.0 11 665.0 12 643.0 13 673.0 14 733.0 15 821.0 16 841.5 17 883.5 18 951.5 19 1023.5 20 1200.0 21 1378.0 22 1529.0 23 1811.0 24 2173.0 25 2628.5 26 3333.0 27 4178.0 28 5019.0 29 6086.5 30 7608.0 31 9687.5 32 12488.5 33 14697.0 34 16656.5 35 19315.0 36 22263.0 37 25200.5 38 27786.0 39 29669.0 40 31538.5 41 33928.5 42 36231.5 43 38457.0 44 40318.5 45 42256.0 46 43441.0 47 44650.5 48 46331.0 49 46291.0 50 44963.5 51 43510.0 52 42141.0 53 41576.5 54 41498.5 55 39264.0 56 35668.5 57 32747.5 58 30047.5 59 26551.5 60 22204.0 61 18343.5 62 14902.5 63 11343.5 64 8495.5 65 6604.0 66 4865.5 67 3493.0 68 2565.0 69 1872.5 70 1395.5 71 1005.0 72 752.5 73 604.0 74 453.5 75 340.0 76 258.0 77 157.0 78 91.0 79 56.5 80 35.5 81 19.0 82 11.0 83 6.5 84 4.0 85 2.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02185671927031072 2 0.009754238352039493 3 0.0014450723484502955 4 0.0 5 9.031702177814347E-5 6 0.0 7 0.0 8 0.0 9 1.8063404355628693E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002167608522675443 26-27 0.00939297026492692 28-29 2.7095106533443037E-4 30-31 0.0 32-33 4.515851088907173E-5 34-35 0.010838042613377214 36-37 9.483287286705063E-4 38-39 0.013502394755832448 40-41 0.013005651136052657 42-43 0.005373862795799537 44-45 6.322191524470043E-4 46-47 9.031702177814347E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 9.031702177814347E-5 66-67 5.419021306688607E-4 68-69 0.0011289627722267934 70-71 4.515851088907173E-5 72-73 3.1610957622350215E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8063404355628693E-4 82-83 0.0010386457504486498 84-85 0.006096398970024684 86-87 0.004109424490905528 88-89 0.0 90-91 0.0015805478811175104 92-93 4.0642659800164553E-4 94-95 9.031702177814347E-5 96-97 4.515851088907173E-5 98-99 0.0 100-101 4.515851088907173E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1107211.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.49150078751227 #Duplication Level Percentage of deduplicated Percentage of total 1 82.38368599865304 53.130475504602956 2 10.608564072244755 13.683244364390939 3 2.855153652010798 5.523994319913687 4 1.3292646557926135 3.429050903834462 5 0.7058377097817451 2.2760266608122635 6 0.4307483128399206 1.6667763094041197 7 0.29601496817511935 1.3363314687226793 8 0.2326100891632429 1.2001098998763664 9 0.1529815342981014 0.8879407865694756 >10 0.9275623382739953 10.78818528565044 >50 0.05402467153637194 2.3979606381677994 >100 0.021729522943458247 2.5650476960224173 >500 0.0014019032976194754 0.5240212728196765 >1k 4.2057098928584264E-4 0.5908348892126614 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2557 0.23094062468671284 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2354 0.21260626926574971 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1624 0.14667484336770498 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.031702177814347E-5 7 0.0 0.0 0.0 0.0 9.031702177814347E-5 8 0.0 0.0 0.0 0.0 9.031702177814347E-5 9 0.0 0.0 0.0 9.031702177814347E-5 9.031702177814347E-5 10-11 0.0 0.0 0.0 1.354755326672152E-4 9.031702177814347E-5 12-13 0.0 0.0 0.0 1.8063404355628693E-4 9.031702177814347E-5 14-15 0.0 0.0 0.0 2.7095106533443037E-4 9.031702177814347E-5 16-17 0.0 0.0 0.0 2.7095106533443037E-4 9.031702177814347E-5 18-19 0.0 0.0 0.0 2.7095106533443037E-4 9.031702177814347E-5 20-21 0.0 0.0 0.0 3.1610957622350215E-4 9.031702177814347E-5 22-23 1.354755326672152E-4 0.0 0.0 4.96743619779789E-4 9.031702177814347E-5 24-25 1.8063404355628693E-4 0.0 0.0 8.580117068923629E-4 9.031702177814347E-5 26-27 1.8063404355628693E-4 0.0 0.0 0.001535389370228439 9.031702177814347E-5 28-29 2.709510653344304E-4 0.0 0.0 0.003702997892903882 9.031702177814347E-5 30-31 3.6126808711257387E-4 0.0 0.0 0.012237956450938439 9.031702177814347E-5 32-33 3.6126808711257387E-4 0.0 0.0 0.029307873567007554 9.031702177814347E-5 34-35 4.064265980016456E-4 0.0 0.0 0.0586157471340151 9.031702177814347E-5 36-37 4.515851088907173E-4 0.0 0.0 0.10512901334975899 9.031702177814347E-5 38-39 5.419021306688607E-4 0.0 0.0 0.17860191056627872 9.031702177814347E-5 40-41 5.870606415579325E-4 0.0 0.0 0.25315861204413614 9.031702177814347E-5 42-43 6.322191524470042E-4 0.0 0.0 0.330515141197116 9.031702177814347E-5 44-45 9.031702177814346E-4 0.0 0.0 0.43361202155686673 9.031702177814347E-5 46-47 9.93487239559578E-4 0.0 0.0 0.5526498562604598 1.354755326672152E-4 48-49 9.93487239559578E-4 0.0 0.0 0.6815774048487597 1.8063404355628693E-4 50-51 9.93487239559578E-4 0.0 0.0 0.8172787300704202 1.8063404355628693E-4 52-53 9.93487239559578E-4 0.0 0.0 0.9513995074109631 2.2579255444535865E-4 54-55 9.93487239559578E-4 0.0 0.0 1.099293630572673 2.7095106533443037E-4 56-57 9.93487239559578E-4 0.0 0.0 1.2706250208858112 3.1610957622350215E-4 58-59 9.93487239559578E-4 0.0 0.0 1.4600649740654672 3.6126808711257387E-4 60-61 9.93487239559578E-4 0.0 0.0 1.6640008092405152 3.6126808711257387E-4 62-63 9.93487239559578E-4 0.0 0.0 1.865317450783997 3.6126808711257387E-4 64-65 9.93487239559578E-4 0.0 0.0 2.0818525104970957 3.6126808711257387E-4 66-67 9.93487239559578E-4 0.0 0.0 2.335507866161012 3.6126808711257387E-4 68-69 9.93487239559578E-4 0.0 0.0 2.6201419602948306 4.515851088907173E-4 70-71 9.93487239559578E-4 0.0 0.0 2.923832946023838 4.515851088907173E-4 72-73 9.93487239559578E-4 0.0 0.0 3.2279303583508474 4.96743619779789E-4 74-75 9.93487239559578E-4 0.0 0.0 3.534737281331201 5.419021306688607E-4 76-77 9.93487239559578E-4 0.0 0.0 3.8697682736172236 5.419021306688607E-4 78-79 9.93487239559578E-4 0.0 0.0 4.257002504491014 5.419021306688607E-4 80-81 0.0012644383048940084 0.0 0.0 4.667628844005343 5.419021306688607E-4 82-83 0.0012644383048940084 0.0 0.0 5.096092795320856 6.322191524470042E-4 84-85 0.0012644383048940084 0.0 0.0 5.51475734977344 6.322191524470042E-4 86-87 0.0012644383048940084 0.0 0.0 5.958530036280347 6.77377663336076E-4 88-89 0.0012644383048940084 0.0 0.0 6.423165954818007 7.225361742251477E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2400 0.0 40.585728 1 GTATCAA 3820 0.0 36.569138 1 TACCTGG 1060 0.0 27.784262 2 TATCAAC 5045 0.0 27.39964 2 ATCAACG 5050 0.0 27.370039 3 TCAACGC 5070 0.0 27.26207 4 CAACGCA 5125 0.0 26.969501 5 AACGCAG 5295 0.0 26.103626 6 GTACCTG 1260 0.0 24.134604 1 TACATGG 4990 0.0 23.989046 2 GTACATG 5110 0.0 23.80399 1 ACGCAGA 5935 0.0 23.288744 7 CGCAGAG 6000 0.0 23.03645 8 ACATGGG 5120 0.0 22.913994 3 TAACGCA 110 0.002878062 21.589924 4 GAGTACT 3125 0.0 21.43102 12-13 AGTACTT 3265 0.0 21.166721 12-13 GTACTTT 3215 0.0 20.53561 14-15 GCAGAGT 6780 0.0 20.03596 9 AGAGTAC 5865 0.0 19.67941 10-11 >>END_MODULE