FastQCFastQC Report
Thu 26 May 2016
SRR938439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579575
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55980.21701249236792883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43180.16739191533489045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29740.11529030945020013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43800.043.3283921
GTATCAA71850.039.255611
ATCAACG90350.030.7432673
TCAACGC91550.030.2363934
TATCAAC93600.029.8282382
CAACGCA92750.029.7939135
AACGCAG97200.028.6017386
GTACATG87250.027.0934771
TACATGG86200.026.319432
ACGCAGA110800.024.7036027
ACATGGG91000.024.2516883
CGCAGAG112850.024.0017228
GCAGAGT120500.021.451829
GAGTACT64600.020.81400312-13
AGAGTAC101350.020.60470210-11
GAGTACA65100.019.5806711
AGTACTT67800.019.30605912-13
GTACTTT69600.019.28236814-15
GTAAGGT9800.018.9279654
CATGGGA42250.018.9124574
Produced by FastQC (version 0.11.5)