##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938439_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2579575 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.794091662386247 31.0 31.0 34.0 27.0 34.0 2 30.843404049116618 31.0 31.0 34.0 27.0 34.0 3 30.877987265344096 31.0 31.0 34.0 27.0 34.0 4 34.52322456218563 37.0 35.0 37.0 30.0 37.0 5 34.48639446420437 37.0 35.0 37.0 30.0 37.0 6 34.48568543267786 37.0 35.0 37.0 30.0 37.0 7 34.37421435701618 37.0 35.0 37.0 30.0 37.0 8 34.339333804987255 37.0 35.0 37.0 30.0 37.0 9 35.78342013703808 38.0 35.0 39.0 30.0 39.0 10-11 35.60202068170145 38.0 35.0 39.0 30.0 39.0 12-13 35.44381729548472 38.0 35.0 39.0 30.0 39.0 14-15 36.34034307977089 39.0 36.0 40.5 29.0 41.0 16-17 36.14562980335908 38.5 35.0 40.0 27.5 41.0 18-19 36.28812265586386 39.0 36.0 40.5 29.0 41.0 20-21 36.25004932983147 39.0 35.0 41.0 27.0 41.0 22-23 36.14657492028725 39.0 35.0 41.0 27.0 41.0 24-25 35.99190234825504 39.0 35.0 41.0 27.0 41.0 26-27 35.76541910973707 39.0 34.5 40.0 27.0 41.0 28-29 35.5361224232674 38.0 34.0 40.0 27.0 41.0 30-31 35.27648953800529 38.0 34.0 40.0 25.5 41.0 32-33 35.00473857127628 38.0 34.0 40.0 25.0 41.0 34-35 34.73489974123645 38.0 33.5 40.0 24.5 41.0 36-37 34.438894585348365 38.0 33.0 40.0 24.0 41.0 38-39 34.294880939689676 38.0 33.0 40.0 24.0 41.0 40-41 34.18888305244081 38.0 33.0 40.0 23.5 41.0 42-43 33.88548869484315 38.0 33.0 40.0 22.0 41.0 44-45 33.52649700047489 38.0 32.5 40.0 19.5 41.0 46-47 33.138842444976405 37.5 31.5 40.0 17.5 41.0 48-49 32.77266022503707 37.0 31.0 40.0 15.0 41.0 50-51 32.1009643061357 36.5 30.5 39.5 13.5 40.5 52-53 32.13918649389919 36.5 31.0 39.0 12.5 40.0 54-55 32.2249667096324 37.0 31.0 39.5 9.0 41.0 56-57 31.875262204045242 36.0 30.5 39.0 8.0 41.0 58-59 31.46557010360234 36.0 30.0 39.0 6.5 40.5 60-61 30.96484808544043 35.5 29.0 39.0 2.0 40.0 62-63 30.367271352839133 35.0 28.0 38.5 2.0 40.0 64-65 29.812096566294834 34.5 28.0 38.0 2.0 40.0 66-67 29.239528410687804 34.0 26.5 38.0 2.0 40.0 68-69 28.609358130699825 34.0 26.0 37.0 2.0 39.5 70-71 27.939637149530448 33.5 25.5 36.5 2.0 39.0 72-73 27.263089462411443 33.0 23.5 36.0 2.0 39.0 74-75 26.55406220016863 32.0 22.0 35.5 2.0 38.0 76-77 25.76783985734084 31.5 19.5 35.0 2.0 37.5 78-79 24.950783753137628 31.0 17.0 35.0 2.0 37.0 80-81 24.224123159822838 31.0 10.0 34.5 2.0 36.0 82-83 23.4634476609519 30.0 6.0 34.0 2.0 36.0 84-85 22.73228865220046 29.5 2.0 34.0 2.0 35.0 86-87 21.9373354137794 29.0 2.0 34.0 2.0 35.0 88-89 21.079860442127092 28.5 2.0 34.0 2.0 35.0 90-91 20.138774604343737 26.5 2.0 33.5 2.0 35.0 92-93 19.163770000872237 25.5 2.0 33.0 2.0 35.0 94-95 18.13072133975558 24.5 2.0 32.5 2.0 34.0 96-97 16.883183470145276 19.0 2.0 32.0 2.0 34.0 98-99 15.38422317629842 2.0 2.0 31.5 2.0 34.0 100-101 12.147329501952841 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6755191398439919 1101 2 0.505268883337866 1101 3 0.5275432105590561 1101 4 0.5122067684341829 1101 5 0.5890915768563474 1101 6 0.67261728425256 1101 7 0.7023701692645048 1101 8 0.7712572024534126 1101 9 0.9819256787167561 1101 10-11 0.91574666235487 1101 12-13 0.8197092581688423 1101 14-15 0.9741462294838783 1101 16-17 0.9348726971642378 1101 18-19 1.0002359222733261 1101 20-21 1.087379838557986 1101 22-23 1.130833329888553 1101 24-25 1.0797185182763656 1101 26-27 0.8842023280102609 1101 28-29 1.1127955433123944 1101 30-31 1.278478346758284 1101 32-33 1.3532160107882731 1101 34-35 1.215024855042408 1101 36-37 1.2499984814724385 1101 38-39 1.322945666783049 1101 40-41 1.3176910255823273 1101 42-43 1.561604949180655 1101 44-45 1.506910063872013 1101 46-47 1.3784371870904835 1101 48-49 1.4129670435443415 1101 50-51 1.4286907838990146 1101 52-53 1.2762257157902468 1101 54-55 1.4654195712348752 1101 56-57 1.331731986391791 1101 58-59 1.4703121567837663 1101 60-61 1.4693234276480105 1101 62-63 1.2737423244875714 1101 64-65 1.2501126481644143 1101 66-67 1.4575069306031843 1101 68-69 1.7431878287450893 1101 70-71 1.6987432673827847 1101 72-73 1.7393528126895 1101 74-75 1.774344175546812 1101 76-77 1.7697084938838046 1101 78-79 1.8592297306936274 1101 80-81 1.9121454186077251 1101 82-83 1.8103276097561114 1101 84-85 1.8254020813525216 1101 86-87 2.041099982247502 1101 88-89 1.819714120221569 1101 90-91 1.7793401389497134 1101 92-93 1.6490718517672427 1101 94-95 1.611612479573445 1101 96-97 1.3673920032246478 1101 98-99 1.4845151557635123 1101 100-101 1.2797150750787516 1102 1 0.575793736167018 1102 2 0.6052677657564729 1102 3 0.588676556426833 1102 4 0.5155963270684225 1102 5 0.5368380725812685 1102 6 0.6134505529414795 1102 7 0.5695886800938723 1102 8 0.6876157672685324 1102 9 0.8941196093294153 1102 10-11 0.8854595491202062 1102 12-13 0.9054280799487984 1102 14-15 1.0388057090064962 1102 16-17 1.2296017559897763 1102 18-19 1.1240537512465565 1102 20-21 1.1490369685300763 1102 22-23 1.124535265671021 1102 24-25 1.1212672362790244 1102 26-27 1.078240754403069 1102 28-29 0.9217693274824512 1102 30-31 1.1228631905138258 1102 32-33 1.3205333289610692 1102 34-35 1.119795415581791 1102 36-37 1.2389012269097535 1102 38-39 1.0532928335827307 1102 40-41 1.328832983400865 1102 42-43 1.3179245432750974 1102 44-45 1.403061442244308 1102 46-47 1.3668866546615561 1102 48-49 1.487765615932723 1102 50-51 1.4554106759274958 1102 52-53 1.4159687509572478 1102 54-55 1.3074803387371858 1102 56-57 1.3700351165400626 1102 58-59 1.4031349964660542 1102 60-61 1.2921852972170491 1102 62-63 1.527975985776262 1102 64-65 1.4697727785138532 1102 66-67 1.0649965666792376 1102 68-69 1.3746854141118732 1102 70-71 1.589093576558394 1102 72-73 1.582537242545488 1102 74-75 1.648393738309526 1102 76-77 1.7334807781281434 1102 78-79 1.8553012677361913 1102 80-81 1.9860921638820024 1102 82-83 1.9197751498279771 1102 84-85 2.027604604193911 1102 86-87 1.9766866993324328 1102 88-89 1.9228502837092663 1102 90-91 1.8651981653535614 1102 92-93 1.6716308896479966 1102 94-95 1.7170253864552478 1102 96-97 2.1808510688477156 1102 98-99 2.392292832009387 1102 100-101 1.8705059085446614 1103 1 0.7066414106091159 1103 2 0.6792673818676782 1103 3 0.6940578186127766 1103 4 0.6592746004780636 1103 5 0.5909338755655043 1103 6 0.6754918437170332 1103 7 0.6954982060922319 1103 8 0.5951042506047273 1103 9 0.784941131347459 1103 10-11 0.9441670687945063 1103 12-13 0.9575558214704145 1103 14-15 1.223606964474385 1103 16-17 1.1414721278567868 1103 18-19 1.0664469127223057 1103 20-21 1.0387413931009704 1103 22-23 1.0860208963598907 1103 24-25 1.1503130326967437 1103 26-27 1.138082103519622 1103 28-29 1.1175922322578202 1103 30-31 1.1919238689362501 1103 32-33 1.2489312814699787 1103 34-35 1.246647615870721 1103 36-37 1.3328755303536397 1103 38-39 1.3176054769096268 1103 40-41 1.280609795002384 1103 42-43 1.3581081658720038 1103 44-45 1.3871870408077598 1103 46-47 1.1690410726659408 1103 48-49 1.1944073340371162 1103 50-51 1.4452590903657594 1103 52-53 1.506932084526298 1103 54-55 1.4953531780999256 1103 56-57 1.6791727747679097 1103 58-59 1.5817634085302679 1103 60-61 1.7586936834192848 1103 62-63 1.7211465536286035 1103 64-65 1.853685091494345 1103 66-67 1.8926735911875063 1103 68-69 1.9309986799952696 1103 70-71 2.2001114690594292 1103 72-73 2.1328432486371973 1103 74-75 2.2384884287739304 1103 76-77 2.2257461766148126 1103 78-79 2.197981998539227 1103 80-81 2.2937638783495586 1103 82-83 2.115147237829433 1103 84-85 2.265650540781543 1103 86-87 2.239123307169823 1103 88-89 2.1795400826222533 1103 90-91 2.197589192710687 1103 92-93 2.0461390321313537 1103 94-95 2.14936135607752 1103 96-97 2.215568538060843 1103 98-99 2.2973452315669025 1103 100-101 1.9580114016902268 1104 1 0.6087942098269821 1104 2 0.4168235548977641 1104 3 0.4697889868647813 1104 4 0.38368202707945187 1104 5 0.49895176394962704 1104 6 0.567163809148262 1104 7 0.4803697432117602 1104 8 0.5780370218402169 1104 9 0.6941539983405036 1104 10-11 0.7859412115585442 1104 12-13 0.7792090543906269 1104 14-15 0.8775093514299925 1104 16-17 1.058668364692572 1104 18-19 0.9419185180825664 1104 20-21 0.9429218886242836 1104 22-23 0.9745061755956002 1104 24-25 0.9526637545538676 1104 26-27 0.929567218585504 1104 28-29 1.1291410121330472 1104 30-31 1.1133970204331334 1104 32-33 1.1925678174623755 1104 34-35 0.9134997392970163 1104 36-37 1.1950509121097141 1104 38-39 1.1300014159119698 1104 40-41 1.170711120857419 1104 42-43 1.2505783151440184 1104 44-45 1.2237324821550288 1104 46-47 1.259632411728063 1104 48-49 1.191546460604691 1104 50-51 1.1590222790826346 1104 52-53 1.3216379632758688 1104 54-55 1.3777663947501821 1104 56-57 1.4636278381815622 1104 58-59 1.5567370831652916 1104 60-61 1.4802093781761378 1104 62-63 1.4794561190931574 1104 64-65 1.6358998188744387 1104 66-67 1.7002216200470919 1104 68-69 1.8560093694104864 1104 70-71 1.9064711657373188 1104 72-73 1.757956345100439 1104 74-75 1.7356596389014953 1104 76-77 1.7224719689179437 1104 78-79 1.7135239181989306 1104 80-81 1.8983179980318035 1104 82-83 1.8333067931252565 1104 84-85 2.0534390784205385 1104 86-87 2.021030139574492 1104 88-89 1.9274733347282798 1104 90-91 2.015022327202523 1104 92-93 2.121552703311462 1104 94-95 2.342386849567088 1104 96-97 2.2931933714407293 1104 98-99 2.22771816351729 1104 100-101 1.9636141560676794 1105 1 0.4212517700684977 1105 2 0.4609486688351332 1105 3 0.24397757363939476 1105 4 0.3619476272136879 1105 5 0.4955670703155306 1105 6 0.4701394206522025 1105 7 0.5338388138586154 1105 8 0.4073280364530376 1105 9 0.6760389397310291 1105 10-11 0.6056818057719582 1105 12-13 0.6334771504414576 1105 14-15 0.7513713978543848 1105 16-17 0.7413443788116325 1105 18-19 0.7306825674450863 1105 20-21 0.6819609607305779 1105 22-23 0.619826026085299 1105 24-25 0.6533320414689143 1105 26-27 0.8074184930672885 1105 28-29 0.7081453808610405 1105 30-31 0.7949334423758714 1105 32-33 0.8111531326286539 1105 34-35 0.7977439598430607 1105 36-37 0.888948937836183 1105 38-39 0.7472308436077313 1105 40-41 0.8828885022249082 1105 42-43 1.0692378698466243 1105 44-45 0.9643714839981286 1105 46-47 0.9169011571017478 1105 48-49 0.8921079107532179 1105 50-51 0.9954645841699943 1105 52-53 0.9480538235324971 1105 54-55 0.9506889330694079 1105 56-57 0.9253398569463833 1105 58-59 1.006494308083564 1105 60-61 0.8545809201824319 1105 62-63 1.023699867722197 1105 64-65 1.211968033603668 1105 66-67 1.2364320740574684 1105 68-69 1.166629293571674 1105 70-71 1.1937226774010945 1105 72-73 1.3311928393163974 1105 74-75 1.3283209798973594 1105 76-77 1.587009284881237 1105 78-79 1.7244634350433081 1105 80-81 1.5863675300911346 1105 82-83 1.7317133303992733 1105 84-85 1.727953113144892 1105 86-87 1.8771559718744157 1105 88-89 1.8413740433903207 1105 90-91 1.6265061653559663 1105 92-93 1.850918132244697 1105 94-95 1.915426274737218 1105 96-97 2.02624739982042 1105 98-99 2.133619441975414 1105 100-101 1.600044706925912 1106 1 0.4468266933922109 1106 2 0.4207180701051598 1106 3 0.3044703318481119 1106 4 0.3485819392931333 1106 5 0.5090861391657029 1106 6 0.2586923268664236 1106 7 0.5024223735776587 1106 8 0.32256423457923944 1106 9 0.2630229376489055 1106 10-11 0.31039388907056775 1106 12-13 0.3575977333683795 1106 14-15 0.5802573930131132 1106 16-17 0.35666259746082574 1106 18-19 0.25068502834428585 1106 20-21 0.3484028010068769 1106 22-23 0.34346860485816677 1106 24-25 0.39470202518955944 1106 26-27 0.39796881023410435 1106 28-29 0.5655140161903631 1106 30-31 0.41709554078575906 1106 32-33 0.5998633889647564 1106 34-35 0.158758723537197 1106 36-37 0.1918182952749916 1106 38-39 0.4127158250347449 1106 40-41 0.534844208307355 1106 42-43 0.019806116421925424 1106 44-45 0.25016509280474963 1106 46-47 0.49912722755037464 1106 48-49 0.6125816565309847 1106 50-51 0.4775187318908536 1106 52-53 0.24847518164266802 1106 54-55 0.3910333909145933 1106 56-57 -0.42046978172670535 1106 58-59 0.05316151909659439 1106 60-61 0.6994490097478199 1106 62-63 0.5872109131337204 1106 64-65 0.60290781358567 1106 66-67 0.07060375749964365 1106 68-69 0.08170176251699601 1106 70-71 0.20803374186474954 1106 72-73 0.7043720432438576 1106 74-75 1.2315167750484832 1106 76-77 1.057625212796168 1106 78-79 1.1443150949878635 1106 80-81 1.2266643009264193 1106 82-83 0.8379311834858569 1106 84-85 0.982072284173416 1106 86-87 0.9002682949122303 1106 88-89 1.0451347963574342 1106 90-91 1.0911539874318166 1106 92-93 0.9306905330900186 1106 94-95 1.0249809427057812 1106 96-97 0.9502443140569525 1106 98-99 1.2680365632563504 1106 100-101 0.6261004695409991 1107 1 0.17227550349790022 1107 2 -0.06383823927508203 1107 3 0.09202518151286299 1107 4 0.14772431506560224 1107 5 0.06849065689137035 1107 6 0.08190400988639368 1107 7 0.1107113619385558 1107 8 0.09100955482846729 1107 9 0.2890230854961331 1107 10-11 0.3835337172367801 1107 12-13 0.3685260153284773 1107 14-15 0.4008137564568912 1107 16-17 0.5545730693472279 1107 18-19 0.5097452220241578 1107 20-21 0.5600254132466418 1107 22-23 0.6169427994493901 1107 24-25 0.599516217640307 1107 26-27 0.366924341320086 1107 28-29 0.5525111839230306 1107 30-31 0.6082435193059865 1107 32-33 0.764160132684836 1107 34-35 0.5655097421935338 1107 36-37 0.5330438935873261 1107 38-39 0.5994830020685598 1107 40-41 0.3878763452183023 1107 42-43 0.6428285218962415 1107 44-45 0.5582842223597382 1107 46-47 0.5753133705142304 1107 48-49 0.600639959090401 1107 50-51 0.5904951116419568 1107 52-53 0.583160609092161 1107 54-55 0.6584222655213949 1107 56-57 0.652611983304336 1107 58-59 0.5951424070846691 1107 60-61 0.6109212095048662 1107 62-63 0.6249704319020459 1107 64-65 0.7072087886050049 1107 66-67 0.8383736972905176 1107 68-69 1.0133009067684426 1107 70-71 1.0993196927375415 1107 72-73 1.1329037713682588 1107 74-75 1.2151217316818013 1107 76-77 1.0145270576867773 1107 78-79 1.0859031672476434 1107 80-81 1.161795366386329 1107 82-83 1.0191619509343113 1107 84-85 1.215966591062326 1107 86-87 1.251134028670279 1107 88-89 1.3545697937811383 1107 90-91 1.0078226309114768 1107 92-93 1.0899389561449162 1107 94-95 1.191314033672647 1107 96-97 1.4039653748938754 1107 98-99 1.490868256846289 1107 100-101 1.3874488472487254 1108 1 0.31588418575467614 1108 2 0.29659524646942614 1108 3 0.2373863196617343 1108 4 0.3359474842910899 1108 5 0.25713602416414005 1108 6 0.2996436894139407 1108 7 0.31058362555808827 1108 8 0.4410342296255436 1108 9 0.43971684263614463 1108 10-11 0.6539352075400018 1108 12-13 0.5196472871847604 1108 14-15 0.6478506677721256 1108 16-17 0.6845498804909695 1108 18-19 0.6306512505814865 1108 20-21 0.46642191133400956 1108 22-23 0.3998744516247257 1108 24-25 0.6829325728047024 1108 26-27 0.7323109858475974 1108 28-29 0.6046238100576034 1108 30-31 0.7744593597077625 1108 32-33 0.8195889288546496 1108 34-35 0.8549510420781985 1108 36-37 0.7975604447868463 1108 38-39 0.9102361695763435 1108 40-41 0.9929590807671858 1108 42-43 1.0738170678827075 1108 44-45 1.0370066404353224 1108 46-47 1.0770356304529969 1108 48-49 1.0262598963264438 1108 50-51 1.1613830089948038 1108 52-53 1.0800283412738239 1108 54-55 1.1720852868616873 1108 56-57 1.071804204063337 1108 58-59 1.2528556668571191 1108 60-61 1.344100700249772 1108 62-63 1.3892422800034474 1108 64-65 1.3120180565133204 1108 66-67 1.4329800729308992 1108 68-69 1.3441187740480274 1108 70-71 1.6731451860289965 1108 72-73 1.708169059518088 1108 74-75 1.6079574520631859 1108 76-77 1.839253590046205 1108 78-79 1.972207359781951 1108 80-81 2.0529982559277578 1108 82-83 1.9996501165744505 1108 84-85 2.1486075305041012 1108 86-87 1.9965058001571165 1108 88-89 2.1430418240125384 1108 90-91 1.88183822920033 1108 92-93 1.9518649944631044 1108 94-95 1.982375213536919 1108 96-97 2.194751042244892 1108 98-99 1.876583866859077 1108 100-101 1.7332752620961376 1109 1 0.4183395275023827 1109 2 0.2600466051838204 1109 3 0.37555102067559787 1109 4 0.3284611921330054 1109 5 0.34549513513731256 1109 6 0.33318738208240006 1109 7 0.2933845945904352 1109 8 0.350656876014952 1109 9 0.47334459282581065 1109 10-11 0.5093457099884446 1109 12-13 0.4850259339879912 1109 14-15 0.4500583631304238 1109 16-17 0.37913377420777294 1109 18-19 0.5138337114408529 1109 20-21 0.45478296209612523 1109 22-23 0.40407840534567185 1109 24-25 0.35658549714430876 1109 26-27 0.5621595960965777 1109 28-29 0.46133979047004203 1109 30-31 0.2936218881580004 1109 32-33 0.6463810783132331 1109 34-35 0.42706398952284985 1109 36-37 0.41661175867056244 1109 38-39 0.3638407285114411 1109 40-41 0.48871115849667746 1109 42-43 0.46886521775510204 1109 44-45 0.4219092630299883 1109 46-47 0.4322413774010556 1109 48-49 0.7798454884362442 1109 50-51 0.7312412750821196 1109 52-53 0.7585290137339911 1109 54-55 0.9927893009316193 1109 56-57 0.7413699930832713 1109 58-59 0.9189572083427358 1109 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-1.7854195842740133 2314 82-83 -1.8096675605307837 2314 84-85 -1.7251203522482612 2314 86-87 -1.619014416733343 2314 88-89 -1.7903173605437601 2314 90-91 -1.8003897045399953 2314 92-93 -1.762539657717781 2314 94-95 -2.0160299998363485 2314 96-97 -2.1303319836992127 2314 98-99 -1.8198855575744837 2314 100-101 -1.8259028638720647 2315 1 -0.6936901090923691 2315 2 -0.6799700893433354 2315 3 -0.5928969759811444 2315 4 -0.6154755602046578 2315 5 -0.45317855324312717 2315 6 -0.669833739650521 2315 7 -0.6890540470366204 2315 8 -0.8300276431304283 2315 9 -1.0709889538143855 2315 10-11 -1.0388390594660137 2315 12-13 -1.0061784226959176 2315 14-15 -1.3761577580801116 2315 16-17 -1.300326134984445 2315 18-19 -1.4392224535628912 2315 20-21 -1.2611915999718164 2315 22-23 -1.2051420560066006 2315 24-25 -1.2461810711400076 2315 26-27 -1.5161936748989149 2315 28-29 -1.522305232079546 2315 30-31 -1.4584905027943051 2315 32-33 -1.5211978561101418 2315 34-35 -1.5424981546029386 2315 36-37 -1.6821123180154345 2315 38-39 -1.7271248550787277 2315 40-41 -1.797973541306554 2315 42-43 -1.922462280111823 2315 44-45 -1.727388946498305 2315 46-47 -1.6811755700470954 2315 48-49 -1.569584895167857 2315 50-51 -1.667984290398735 2315 52-53 -1.5893804701504592 2315 54-55 -1.585101442421042 2315 56-57 -1.7024260964359605 2315 58-59 -1.744400481152116 2315 60-61 -1.9451214711932074 2315 62-63 -1.8667602860993036 2315 64-65 -1.8578171692223044 2315 66-67 -2.049420651461258 2315 68-69 -1.923657659830834 2315 70-71 -2.0333329101696513 2315 72-73 -2.174478360064665 2315 74-75 -2.1919127982171958 2315 76-77 -2.273852116577597 2315 78-79 -2.6667532766745694 2315 80-81 -2.703819185691433 2315 82-83 -2.588155182677255 2315 84-85 -2.5183397133649628 2315 86-87 -2.67102269093769 2315 88-89 -2.9145343514174904 2315 90-91 -2.7471897904354776 2315 92-93 -2.9846283617734173 2315 94-95 -3.2564654569592015 2315 96-97 -3.4600211340571345 2315 98-99 -3.198297086459915 2315 100-101 -2.5677140138531502 2316 1 -1.0093575824863947 2316 2 -0.9490320283739777 2316 3 -0.7864334069338312 2316 4 -0.8959837784057143 2316 5 -0.8915936345035433 2316 6 -0.8412479433945919 2316 7 -0.9878303324578255 2316 8 -0.9882530566449788 2316 9 -1.094306746960946 2316 10-11 -1.0434062014874215 2316 12-13 -1.275785303385753 2316 14-15 -1.4763826967741736 2316 16-17 -1.3829115926552973 2316 18-19 -1.3053323414491587 2316 20-21 -1.2156233580497755 2316 22-23 -1.3467480010796962 2316 24-25 -1.670545623085772 2316 26-27 -1.5635550152951083 2316 28-29 -1.7029967169654228 2316 30-31 -1.72836885258274 2316 32-33 -1.7536062077417611 2316 34-35 -1.5755730580360705 2316 36-37 -1.5242092114472783 2316 38-39 -2.0630628559442243 2316 40-41 -2.121185551660446 2316 42-43 -2.0475664601349024 2316 44-45 -1.8216197773740603 2316 46-47 -1.931644881016453 2316 48-49 -2.0094099127983256 2316 50-51 -2.037270667257946 2316 52-53 -2.2039604643804864 2316 54-55 -2.2448474835801626 2316 56-57 -2.178615451641754 2316 58-59 -2.355815255882849 2316 60-61 -2.4096436655450475 2316 62-63 -2.4381443878270908 2316 64-65 -2.351291893545934 2316 66-67 -2.5679784693706047 2316 68-69 -2.6840603857412084 2316 70-71 -2.8797935942317103 2316 72-73 -2.7499490233708563 2316 74-75 -2.818154443780731 2316 76-77 -3.121737011676327 2316 78-79 -3.2221129381696336 2316 80-81 -3.3194319645733437 2316 82-83 -3.3743364539139513 2316 84-85 -3.331643852918109 2316 86-87 -3.3028632614435267 2316 88-89 -3.753388391198232 2316 90-91 -3.3954842933364873 2316 92-93 -3.4276792036281414 2316 94-95 -3.9225334224034896 2316 96-97 -3.8177222415709196 2316 98-99 -3.676430300654042 2316 100-101 -3.1169310609104652 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13621.0 3 5098.0 4 5419.0 5 5906.0 6 6850.0 7 8257.0 8 10204.0 9 12487.0 10 15220.0 11 17970.0 12 21242.0 13 23721.0 14 26558.0 15 29325.0 16 32881.0 17 36555.0 18 36359.0 19 36399.0 20 39337.0 21 42648.0 22 47160.0 23 51077.0 24 57143.0 25 63045.0 26 69906.0 27 77793.0 28 86984.0 29 97942.0 30 111183.0 31 127178.0 32 147037.0 33 171204.0 34 200870.0 35 237533.0 36 265003.0 37 238344.0 38 99665.0 39 4451.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45633466348608 18.40747645869994 14.158508771215663 23.97768010659831 2 15.62948457944977 19.47396974487812 40.02862369542153 24.867921980250582 3 19.6308206503853 23.28679296833765 28.935478412597877 28.146907968679173 4 13.099678256920969 15.783574414245185 36.14797076756488 34.96877656126897 5 14.213480397676312 36.929726405343764 33.88204205528736 14.974751141692572 6 33.53868587308522 35.103837739805385 16.929652948128858 14.427823438980536 7 29.73708782558657 31.637754935855977 20.05693160297245 18.568225635585005 8 26.80112871185004 35.01957641120972 19.211445017176136 18.967849859764105 9 26.4676652796826 16.546405803541482 19.674739247383748 37.311189669392164 10-11 25.428704242215737 26.14280890995044 26.967957309250973 21.460529538582847 12-13 26.172010905295444 23.27756567865062 27.291785693645597 23.258637722408334 14-15 22.93095746438174 24.829033911699447 25.415219975849922 26.824788648068886 16-17 22.812192018296983 28.083820852878226 26.120900710819107 22.983086418005687 18-19 22.853014978669904 26.852982403276776 28.014896261915467 22.279106356137852 20-21 23.327171179739523 26.003460684076625 28.27260974445164 22.396758391732217 22-23 22.989532031046565 25.94444680344885 28.06376989973714 23.00225126576744 24-25 22.91531566193285 26.110545283610154 28.140814512350637 22.833324542106357 26-27 22.577848698791612 26.445925011112653 27.998554773166102 22.977671516929636 28-29 22.673044171018166 26.7117854930652 27.963303492436086 22.651866843480544 30-31 22.704244518528117 26.321015134223423 27.9500757603382 23.02466458691026 32-33 22.537099998371023 26.315458778684576 27.941179892813178 23.206261330131227 34-35 23.135189251608693 26.453196887692627 27.793991752594916 22.61762210810377 36-37 23.14071848623138 26.082864605271627 27.74540444812652 23.031012460370466 38-39 22.69825527793693 26.483828092962653 27.76160409341248 23.056312535687937 40-41 23.117330193199294 26.232225011570936 27.767306139994886 22.883138655234884 42-43 22.817741806952306 26.202318830800813 27.72692962674064 23.253009735506243 44-45 22.792894430261477 26.480730871893883 27.771271058798902 22.955103639045742 46-47 23.094948786882245 26.659705911317445 27.220010530307203 23.025334771493107 48-49 22.941456181742513 26.544139269654675 27.344091505525732 23.17031304307708 50-51 23.035862058537948 26.81234697083919 27.192226627476213 22.95956434314665 52-53 23.149893867581632 26.66926424549058 27.14411716243591 23.036724724491872 54-55 22.803685343841618 26.730955477032897 27.384040153561163 23.08131902556432 56-57 22.873974709683225 26.799442542159735 27.282885427835712 23.043697320321332 58-59 22.92022532705749 26.493454779010705 27.27299163602123 23.313328257910573 60-61 22.745569426376186 26.680515945411997 27.39999340818277 23.173921220029044 62-63 22.784117905109326 26.69129097123264 27.481158122889802 23.043433000768232 64-65 22.554623901246153 26.62372290612821 27.42534192337776 23.396311269247878 66-67 22.584951752731442 26.698753972466356 27.31705718994602 23.399237084856182 68-69 22.805965982886764 26.413644840246736 27.458254015639966 23.32213516122654 70-71 22.713369176808637 26.471621545638385 27.217451494005946 23.59755778354703 72-73 22.771283242418455 26.46986915818995 27.203579852086808 23.555267747304786 74-75 22.592542828741454 26.864317050278096 27.09306273172909 23.450077389251355 76-77 22.79691469860925 26.468877017373966 27.13341689277153 23.60079139124526 78-79 22.833707605989268 26.510909797543853 27.1960759038515 23.459306692615375 80-81 22.810799117564333 26.613797545718537 27.004965978232192 23.570437358484934 82-83 22.79997014812539 26.508561575791294 27.10286587688428 23.58860239919904 84-85 22.827865229871243 26.445834838825732 27.032647345707062 23.693652585595963 86-87 22.800849932548577 26.471200015043056 26.980453916399007 23.747496136009367 88-89 22.8125195718897 26.471936988355658 26.92944657337052 23.786096866384128 90-91 22.775282434799806 26.437293318222316 26.885175335950294 23.902248911027584 92-93 22.833641286539958 26.119382439105728 27.058074222862473 23.988902051491845 94-95 22.703778585083224 26.03047472481955 27.155552728650605 24.11019396144662 96-97 22.900779345284807 25.801407440458355 27.02579648211632 24.272016732140514 98-99 22.729132196715156 25.872888713450543 26.823105799017195 24.57487329081711 100-101 23.36649638042323 25.348093672757514 26.984094655797364 24.301315291021886 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 262.0 1 192.5 2 168.5 3 222.5 4 216.5 5 240.0 6 408.0 7 808.0 8 1297.0 9 1535.0 10 1426.0 11 1305.0 12 1391.0 13 1499.5 14 1608.5 15 1782.5 16 1934.5 17 2109.5 18 2364.0 19 2633.5 20 2956.0 21 3493.5 22 4214.0 23 5253.0 24 6610.5 25 8350.5 26 10825.0 27 13994.0 28 17053.5 29 20515.0 30 24722.5 31 29699.0 32 36171.0 33 40508.0 34 43345.5 35 47745.0 36 53290.0 37 58780.5 38 63030.5 39 67052.0 40 71291.5 41 76241.5 42 80269.0 43 84411.0 44 88979.0 45 92986.0 46 96080.0 47 98943.0 48 101996.5 49 102335.0 50 100788.5 51 98416.0 52 95792.0 53 94533.5 54 93450.5 55 88824.5 56 81870.5 57 75304.5 58 68863.0 59 61253.0 60 51647.0 61 42691.0 62 35117.5 63 27639.0 64 21390.0 65 16692.5 66 12670.0 67 9561.0 68 7358.5 69 5513.5 70 4212.0 71 3228.0 72 2294.5 73 1745.5 74 1376.5 75 1000.5 76 716.0 77 460.0 78 275.5 79 170.0 80 110.0 81 66.5 82 39.0 83 21.0 84 13.0 85 14.5 86 10.0 87 7.0 88 4.5 89 4.5 90 6.0 91 4.0 92 1.5 93 1.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006667765038814533 2 0.10424197784518767 3 0.1119176608549858 4 0.1157167362840778 5 0.15556826221373676 6 0.09230202649661282 7 0.02678735838267932 8 0.027368849519785236 9 0.02318211333262262 10-11 0.05084170842096082 12-13 0.05341965246213039 14-15 0.02717501914074993 16-17 0.030605816849674852 18-19 0.03649826037234816 20-21 0.05706366358799415 22-23 0.031148541910973705 24-25 0.04834129653140537 26-27 0.05555178663151876 28-29 0.05217913803630443 30-31 0.06625122355426766 32-33 0.04934921450238896 34-35 0.06016494965255905 36-37 0.0512099861411279 38-39 0.030915945456131338 40-41 0.035529108477171625 42-43 0.03173003304807963 44-45 0.007365554403341635 46-47 0.013684424759892618 48-49 0.009051878700948798 50-51 0.007966428578351085 52-53 0.00969151895176531 54-55 0.010738202998555962 56-57 0.014207766783287943 58-59 0.018588333349485866 60-61 0.02403496700037797 62-63 0.010873884263880678 64-65 0.009013112625141739 66-67 0.010001647558221801 68-69 0.011048331605012455 70-71 0.02984987837143716 72-73 0.027039337875425215 74-75 0.028241086225444117 76-77 0.02219357839954256 78-79 0.004942674665400308 80-81 0.0047876103621720635 82-83 0.006687148076718064 84-85 0.005078355930725023 86-87 0.012075632613899576 88-89 0.0364594942965411 90-91 0.03421106189973155 92-93 0.027911574581084096 94-95 0.04558890514910402 96-97 0.04126648769661669 98-99 0.02473275636490507 100-101 0.07196921973580919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2579575.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.559378770151106 #Duplication Level Percentage of deduplicated Percentage of total 1 78.09554221608362 46.5132197910805 2 12.50962269718534 14.901307129866822 3 4.107441673478278 7.3391002322098835 4 1.8348558918461308 4.371315082044284 5 0.9796204991385684 2.917279417959924 6 0.5774408354315461 2.0635210460891966 7 0.3716868650326181 1.5496207144857417 8 0.2574736621249486 1.226797709267018 9 0.19326795082509293 1.0359827178590384 >10 0.9819703866432099 10.431007067573363 >50 0.0592262664661826 2.429587672668692 >100 0.028712124830602422 3.1666893675571934 >500 0.001961831821208834 0.7899533723614652 >1k 0.0011117046986741563 1.04658500485972 >5k 6.539439403965626E-5 0.21803367411725327 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5598 0.21701249236792883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4318 0.16739191533489045 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2974 0.11529030945020013 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.876607580706124E-5 2 0.0 0.0 0.0 0.0 3.876607580706124E-5 3 0.0 0.0 0.0 0.0 3.876607580706124E-5 4 0.0 0.0 0.0 0.0 3.876607580706124E-5 5 0.0 0.0 0.0 0.0 3.876607580706124E-5 6 0.0 0.0 0.0 0.0 7.753215161412248E-5 7 0.0 0.0 0.0 0.0 7.753215161412248E-5 8 0.0 0.0 0.0 0.0 7.753215161412248E-5 9 0.0 0.0 0.0 0.0 1.1629822742118372E-4 10-11 0.0 0.0 0.0 0.0 1.1629822742118372E-4 12-13 0.0 0.0 0.0 0.0 2.1321341693883683E-4 14-15 0.0 0.0 0.0 5.814911371059186E-5 2.907455685529593E-4 16-17 0.0 0.0 0.0 1.1629822742118372E-4 3.101286064564899E-4 18-19 0.0 0.0 0.0 1.1629822742118372E-4 3.101286064564899E-4 20-21 0.0 0.0 0.0 1.1629822742118372E-4 3.295116443600205E-4 22-23 0.0 0.0 0.0 2.907455685529593E-4 3.4889468226355114E-4 24-25 0.0 0.0 0.0 4.0704379597414304E-4 3.4889468226355114E-4 26-27 0.0 0.0 0.0 8.334706298518166E-4 4.2642683387767366E-4 28-29 0.0 0.0 0.0 0.0030237539129507767 4.2642683387767366E-4 30-31 0.0 0.0 0.0 0.010718819960652434 4.2642683387767366E-4 32-33 0.0 0.0 0.0 0.026012036866538094 4.651929096847349E-4 34-35 0.0 0.0 0.0 0.051946541581462065 5.039589854917961E-4 36-37 0.0 0.0 0.0 0.09724470116201311 5.039589854917961E-4 38-39 0.0 0.0 0.0 0.16772142697925047 5.039589854917961E-4 40-41 0.0 0.0 0.0 0.24180339784654448 5.039589854917961E-4 42-43 0.0 0.0 0.0 0.31578845352432083 5.039589854917961E-4 44-45 0.0 0.0 0.0 0.4080517139451266 5.62108099202388E-4 46-47 0.0 0.0 0.0 0.5205120998614113 6.202572129129798E-4 48-49 0.0 0.0 0.0 0.6476260624327651 7.17172402430633E-4 50-51 0.0 0.0 0.0 0.779818380934844 7.365554403341636E-4 52-53 0.0 0.0 0.0 0.9101305447602803 7.365554403341636E-4 54-55 0.0 0.0 0.0 1.0521888295552562 7.365554403341636E-4 56-57 0.0 0.0 0.0 1.225550720564434 7.365554403341636E-4 58-59 0.0 1.938303790353062E-5 0.0 1.4133917408875494 7.365554403341636E-4 60-61 0.0 3.876607580706124E-5 0.0 1.6151110163495925 7.559384782376943E-4 62-63 0.0 3.876607580706124E-5 0.0 1.814097283467238 8.528536677553473E-4 64-65 0.0 3.876607580706124E-5 0.0 2.027795276353663 8.916197435624085E-4 66-67 0.0 3.876607580706124E-5 0.0 2.2698894197687602 8.916197435624085E-4 68-69 0.0 3.876607580706124E-5 0.0 2.5334793522188734 8.916197435624085E-4 70-71 0.0 3.876607580706124E-5 0.0 2.8237015787484374 8.916197435624085E-4 72-73 0.0 3.876607580706124E-5 0.0 3.119913163990192 0.0010273010088871228 74-75 0.0 7.753215161412248E-5 0.0 3.4197106112441 0.0010466840467906536 76-77 0.0 7.753215161412248E-5 0.0 3.7426126396790167 0.0010854501225977147 78-79 0.0 7.753215161412248E-5 0.0 4.112130874271925 0.0010854501225977147 80-81 0.0 7.753215161412248E-5 0.0 4.504327263211963 0.0011629822742118372 82-83 0.0 7.753215161412248E-5 0.0 4.905362317436012 0.001279280501633021 84-85 0.0 7.753215161412248E-5 0.0 5.3044203017939004 0.001279280501633021 86-87 0.0 7.753215161412248E-5 0.0 5.717123944835874 0.001279280501633021 88-89 0.0 7.753215161412248E-5 0.0 6.15624666854036 0.001279280501633021 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4380 0.0 43.328392 1 GTATCAA 7185 0.0 39.25561 1 ATCAACG 9035 0.0 30.743267 3 TCAACGC 9155 0.0 30.236393 4 TATCAAC 9360 0.0 29.828238 2 CAACGCA 9275 0.0 29.793913 5 AACGCAG 9720 0.0 28.601738 6 GTACATG 8725 0.0 27.093477 1 TACATGG 8620 0.0 26.31943 2 ACGCAGA 11080 0.0 24.703602 7 ACATGGG 9100 0.0 24.251688 3 CGCAGAG 11285 0.0 24.001722 8 GCAGAGT 12050 0.0 21.45182 9 GAGTACT 6460 0.0 20.814003 12-13 AGAGTAC 10135 0.0 20.604702 10-11 GAGTACA 6510 0.0 19.580671 1 AGTACTT 6780 0.0 19.306059 12-13 GTACTTT 6960 0.0 19.282368 14-15 GTAAGGT 980 0.0 18.927965 4 CATGGGA 4225 0.0 18.912457 4 >>END_MODULE