FastQCFastQC Report
Thu 26 May 2016
SRR938439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579575
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57490.22286616981479507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47390.18371243324966322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33020.1280055823149162No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46050.045.4094771
GTATCAA72650.035.324951
ATCAACG91900.027.5501863
TCAACGC92900.027.3047624
TATCAAC95250.026.8435972
CAACGCA96000.026.4725275
AACGCAG100400.025.3956766
GTACATG91300.022.4351981
ACGCAGA115950.021.9489237
TACATGG90650.021.8620912
CGCAGAG117550.021.5693868
ACATGGG89700.020.9708273
CTATGCG1656.324463E-520.1524074
AGAGTAC110950.019.12931410-11
GGACCGT2502.1838423E-718.992376
GCAGAGT131200.018.9272299
GAGTACT67750.018.4317112-13
GAGTACA71050.018.3946721
AGTACTT71750.017.93356312-13
GTACTTT70600.017.58677314-15
Produced by FastQC (version 0.11.5)