##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938439_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2579575 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.754134305069634 31.0 31.0 34.0 30.0 34.0 2 31.977716484304587 31.0 31.0 34.0 30.0 34.0 3 32.04550517042536 33.0 31.0 34.0 30.0 34.0 4 35.72717947723947 37.0 35.0 37.0 33.0 37.0 5 35.61828983456577 37.0 35.0 37.0 33.0 37.0 6 35.61560644679841 37.0 35.0 37.0 33.0 37.0 7 35.546925753273314 37.0 35.0 37.0 33.0 37.0 8 35.52449066222149 37.0 35.0 37.0 33.0 37.0 9 37.09849684541058 39.0 37.0 39.0 33.0 39.0 10-11 36.993712142504094 39.0 37.0 39.0 33.0 39.0 12-13 36.886419274492894 39.0 37.0 39.0 33.0 39.0 14-15 37.92747332409408 40.0 37.0 41.0 33.0 41.0 16-17 37.83236579669131 40.0 37.0 41.0 32.5 41.0 18-19 38.065255904557915 40.0 37.0 41.0 33.0 41.0 20-21 38.02571411957396 40.0 37.0 41.0 33.0 41.0 22-23 37.917727726466566 40.0 37.0 41.0 32.5 41.0 24-25 37.80104997916323 40.0 37.0 41.0 32.0 41.0 26-27 37.61357413527422 40.0 37.0 41.0 32.0 41.0 28-29 37.49898801159106 39.0 36.5 41.0 32.0 41.0 30-31 37.25633912563116 39.0 36.0 41.0 31.0 41.0 32-33 37.071661998585036 39.0 36.0 41.0 31.0 41.0 34-35 36.996539546243085 39.0 36.0 41.0 30.5 41.0 36-37 36.966830194896446 39.0 36.0 41.0 31.0 41.0 38-39 36.8144665303393 39.0 35.0 41.0 30.0 41.0 40-41 36.65371059186106 39.0 35.0 40.5 30.0 41.0 42-43 36.455665177403255 39.0 35.0 40.0 30.0 41.0 44-45 36.18210189663026 38.5 35.0 40.0 30.0 41.0 46-47 35.831844005310955 38.0 35.0 40.0 28.5 41.0 48-49 35.505522033668335 38.0 34.0 40.0 27.5 41.0 50-51 35.14045937799831 38.0 34.0 40.0 26.5 41.0 52-53 34.647916226510176 38.0 33.0 40.0 26.0 41.0 54-55 34.302009633369835 37.5 33.0 40.0 24.5 41.0 56-57 33.969608753379916 37.0 33.0 40.0 24.0 41.0 58-59 33.489119719333615 36.5 32.0 39.0 23.0 41.0 60-61 33.08431718403225 36.0 31.5 39.0 22.0 40.0 62-63 32.589484120446194 35.5 31.0 39.0 20.5 40.0 64-65 32.05018249130186 35.0 30.5 38.5 19.0 40.0 66-67 31.53754106781156 35.0 30.0 38.0 18.0 40.0 68-69 31.022731651531743 34.0 29.0 37.5 15.0 39.5 70-71 30.430412955622536 34.0 29.0 37.0 10.5 39.0 72-73 29.914638264055124 34.0 28.5 36.0 8.0 39.0 74-75 29.304626343486817 33.5 28.0 36.0 7.0 38.5 76-77 28.099485380343662 31.5 26.0 34.5 6.0 36.5 78-79 28.782812284969424 33.0 28.0 35.0 2.0 37.0 80-81 28.39297190428656 33.0 27.0 35.0 2.0 37.0 82-83 27.932163437775603 33.0 26.0 35.0 2.0 36.0 84-85 27.35086380508417 32.5 26.0 35.0 2.0 36.0 86-87 26.717586230289875 32.0 25.0 34.5 2.0 35.0 88-89 25.949316457168333 31.5 23.5 34.0 2.0 35.0 90-91 25.3079049843482 31.0 21.0 34.0 2.0 35.0 92-93 24.437741682253858 31.0 18.0 34.0 2.0 35.0 94-95 23.58610236182317 30.5 6.0 34.0 2.0 35.0 96-97 22.480840642353876 30.0 2.0 34.0 2.0 35.0 98-99 21.075329075526007 29.0 2.0 34.0 2.0 35.0 100-101 17.168822189701793 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2504545191438403 1101 2 0.28218581635002593 1101 3 0.27830985337553926 1101 4 0.22865176236003038 1101 5 0.12671286308862761 1101 6 0.22338472343752613 1101 7 0.21786262098883924 1101 8 0.2539486158246831 1101 9 0.2522230224565547 1101 10-11 0.26217866373981025 1101 12-13 0.3232926121180597 1101 14-15 0.3508000326684808 1101 16-17 0.3698148502936007 1101 18-19 0.4253501307313101 1101 20-21 0.4086459347594982 1101 22-23 0.3427841093156303 1101 24-25 0.45067394030539987 1101 26-27 0.26799387093684146 1101 28-29 0.2734703422317537 1101 30-31 0.4552250983037851 1101 32-33 0.49080871745178456 1101 34-35 0.524750303628231 1101 36-37 0.542893357689735 1101 38-39 0.46596984610762604 1101 40-41 0.6122585014642112 1101 42-43 0.6616195151570423 1101 44-45 0.6917828576273521 1101 46-47 0.6364050616282526 1101 48-49 0.631587813282124 1101 50-51 0.7829023264961634 1101 52-53 0.7027467783925516 1101 54-55 0.7075206451002813 1101 56-57 0.6130182745784722 1101 58-59 0.7646750760842238 1101 60-61 0.6973574279426842 1101 62-63 0.8475154475808324 1101 64-65 0.785678822645913 1101 66-67 0.6348851850665511 1101 68-69 0.6298855372526155 1101 70-71 0.811365324863953 1101 72-73 0.788988320878417 1101 74-75 0.7466783070361878 1101 76-77 0.724623152853777 1101 78-79 0.9397087368717578 1101 80-81 0.7802420429532013 1101 82-83 0.7083984557811362 1101 84-85 1.0098426977411066 1101 86-87 1.1492275204803875 1101 88-89 1.3411734720841046 1101 90-91 1.1497033569418704 1101 92-93 1.2252385377582513 1101 94-95 1.073530910768703 1101 96-97 1.0725940994301517 1101 98-99 1.3024012134915566 1101 100-101 1.5187598437855172 1102 1 0.3072227235586844 1102 2 0.2859655423716596 1102 3 0.22901235189310398 1102 4 0.20817865728503193 1102 5 0.16413869184097507 1102 6 0.16525701412439275 1102 7 0.21605739833375992 1102 8 0.27393688779403647 1102 9 0.17750547471939626 1102 10-11 0.3897568254104513 1102 12-13 0.2695002968706959 1102 14-15 0.4860009212114278 1102 16-17 0.5042094367293117 1102 18-19 0.40150373769240133 1102 20-21 0.4287252567425881 1102 22-23 0.41687219576646584 1102 24-25 0.4559464592418223 1102 26-27 0.49221439078311136 1102 28-29 0.4635323154071713 1102 30-31 0.4849936866407063 1102 32-33 0.4689256519654563 1102 34-35 0.5143199821986784 1102 36-37 0.6613841034583743 1102 38-39 0.5862275209335124 1102 40-41 0.51636097841903 1102 42-43 0.4790916840932695 1102 44-45 0.6262529794174228 1102 46-47 0.6372190881951383 1102 48-49 0.5616252456848869 1102 50-51 0.6305784474631722 1102 52-53 0.5174872825663854 1102 54-55 0.5164779942498043 1102 56-57 0.6530365634693212 1102 58-59 0.794050374379843 1102 60-61 0.8364645921653562 1102 62-63 0.8066053944759872 1102 64-65 0.8084276850137897 1102 66-67 0.7743590145972661 1102 68-69 0.908629073359041 1102 70-71 1.0506279972508814 1102 72-73 1.0146321135026426 1102 74-75 0.9562189021942977 1102 76-77 0.8348848838427294 1102 78-79 1.1898400526858026 1102 80-81 1.1904540744400727 1102 82-83 1.2608897748032888 1102 84-85 1.2261191860636949 1102 86-87 1.0564722090104546 1102 88-89 1.114677030594752 1102 90-91 1.2435519259775027 1102 92-93 1.577365919685775 1102 94-95 1.5351769964069923 1102 96-97 1.590045625980597 1102 98-99 1.6675028936765734 1102 100-101 1.7838736530156858 1103 1 0.3022949931496406 1103 2 0.2662372573443328 1103 3 0.29304759136899605 1103 4 0.11818356954623965 1103 5 0.14109999736594148 1103 6 0.1391648883907095 1103 7 0.24966850383245998 1103 8 0.20446722258547112 1103 9 0.3768567784259389 1103 10-11 0.33501463072832394 1103 12-13 0.3820734756719588 1103 14-15 0.50441463967951 1103 16-17 0.50716515945048 1103 18-19 0.463051434612197 1103 20-21 0.5028618857948857 1103 22-23 0.45114216303489485 1103 24-25 0.5343333307502078 1103 26-27 0.5627118108988824 1103 28-29 0.4702486329539184 1103 30-31 0.46352195929267026 1103 32-33 0.356741570975025 1103 34-35 0.48910928533948805 1103 36-37 0.5157095136132384 1103 38-39 0.4318016240985756 1103 40-41 0.5218509670033882 1103 42-43 0.6349465880200498 1103 44-45 0.5220997825329832 1103 46-47 0.560105742503282 1103 48-49 0.8181464618546883 1103 50-51 0.7563313872431507 1103 52-53 0.6779018130097825 1103 54-55 0.6242112066918324 1103 56-57 0.6236559693003798 1103 58-59 0.6543134329127867 1103 60-61 0.832922460370277 1103 62-63 1.0367495674481972 1103 64-65 0.9748872011623746 1103 66-67 0.9694820168601836 1103 68-69 0.9949158142947176 1103 70-71 1.0229556450733241 1103 72-73 0.7830006871537272 1103 74-75 0.6977436731238136 1103 76-77 0.7454189059870551 1103 78-79 0.9992364254418185 1103 80-81 1.2733912593734473 1103 82-83 1.2135780065913018 1103 84-85 1.3383424136087285 1103 86-87 1.352705079235836 1103 88-89 1.2757168009686382 1103 90-91 1.324664060651756 1103 92-93 1.7900415740646345 1103 94-95 1.6153117300083721 1103 96-97 1.8744560788683486 1103 98-99 2.1398651104323108 1103 100-101 1.9836277873969692 1104 1 0.27427632067086805 1104 2 0.29581615564235975 1104 3 0.2973761919560829 1104 4 0.17613199204583196 1104 5 0.07225082164543295 1104 6 0.13337039837076503 1104 7 0.1972386067938885 1104 8 0.16489019876370037 1104 9 0.2506221356545595 1104 10-11 0.2651501902778577 1104 12-13 0.3935992074527803 1104 14-15 0.46574330642458506 1104 16-17 0.45064855195845155 1104 18-19 0.39591876763679323 1104 20-21 0.48262029888535096 1104 22-23 0.4715453163204941 1104 24-25 0.3533785301307759 1104 26-27 0.353233040873846 1104 28-29 0.3611033481805421 1104 30-31 0.3640173631504169 1104 32-33 0.4835966745735263 1104 34-35 0.6553133055397282 1104 36-37 0.5188448967414843 1104 38-39 0.4595878247679934 1104 40-41 0.5373928866630848 1104 42-43 0.565523813378654 1104 44-45 0.5996182577629483 1104 46-47 0.6689461951709177 1104 48-49 0.6745558382789127 1104 50-51 0.7339125594156286 1104 52-53 0.6637698088393762 1104 54-55 0.776823895793413 1104 56-57 0.6999937848372255 1104 58-59 0.6353618501442497 1104 60-61 0.7931657992412724 1104 62-63 0.6719037461825721 1104 64-65 0.5924322529126229 1104 66-67 0.7526587916093916 1104 68-69 0.746110716331895 1104 70-71 0.6787367962957767 1104 72-73 0.8786565578173189 1104 74-75 1.0816587679257559 1104 76-77 1.1045925988767813 1104 78-79 1.1206527147629401 1104 80-81 1.0040732042466942 1104 82-83 1.3025604967778683 1104 84-85 1.4211254258308017 1104 86-87 1.4248688159613572 1104 88-89 1.546395918813161 1104 90-91 1.4539391375910427 1104 92-93 1.66312054219609 1104 94-95 1.8799435945119214 1104 96-97 1.936334316321613 1104 98-99 2.1611966195204566 1104 100-101 2.157150687861055 1105 1 0.20749787958752464 1105 2 0.20185048847843134 1105 3 0.16269326337897638 1105 4 0.169544817176984 1105 5 0.15807198782226095 1105 6 0.18475123626814138 1105 7 0.15034272828979311 1105 8 0.22211843792774033 1105 9 0.2603759610225467 1105 10-11 0.17890080311453005 1105 12-13 0.2720982754167025 1105 14-15 0.36510656803527297 1105 16-17 0.2809170465169899 1105 18-19 0.238446617294521 1105 20-21 0.2736606923592575 1105 22-23 0.15431047031837863 1105 24-25 0.23347272126939345 1105 26-27 0.12134525924208361 1105 28-29 0.18428587781300365 1105 30-31 0.2735626711155916 1105 32-33 0.4455415931197777 1105 34-35 0.4068680098968329 1105 36-37 0.41661128733669983 1105 38-39 0.379714123741401 1105 40-41 0.4115754433870791 1105 42-43 0.5540999261633246 1105 44-45 0.6006785416647205 1105 46-47 0.44980432381024826 1105 48-49 0.5845887938164722 1105 50-51 0.6224267549413298 1105 52-53 0.6800197028765282 1105 54-55 0.7960479593658718 1105 56-57 0.6151634258919003 1105 58-59 0.7580397344529217 1105 60-61 0.8974625161845822 1105 62-63 0.8060116351492397 1105 64-65 0.6815904840628093 1105 66-67 0.5800431304318252 1105 68-69 0.6207365691689439 1105 70-71 0.7779645565514492 1105 72-73 0.9005893376094818 1105 74-75 0.8386551560128233 1105 76-77 0.8545196265250006 1105 78-79 1.0261902238626348 1105 80-81 0.8732318111826523 1105 82-83 0.9042848290808507 1105 84-85 0.9742628436932108 1105 86-87 0.9664396791063012 1105 88-89 1.0437162604461143 1105 90-91 1.227935680362016 1105 92-93 1.229580439300701 1105 94-95 1.2945355741950166 1105 96-97 1.2610802895149789 1105 98-99 1.2786427794706086 1105 100-101 1.316720028636695 1106 1 0.020936487240238222 1106 2 0.09662493345777179 1106 3 0.08162591355761606 1106 4 0.027334612156863614 1106 5 -0.03480268833417455 1106 6 0.03484987069590062 1106 7 0.02405550506298937 1106 8 -0.020535579490207567 1106 9 -0.15016298157329544 1106 10-11 0.03541237642862427 1106 12-13 0.045320318269226334 1106 14-15 -0.03498999674223313 1106 16-17 0.09157098228298821 1106 18-19 0.1034579323276077 1106 20-21 0.11360528243525891 1106 22-23 0.18512770432437975 1106 24-25 0.20716207980823498 1106 26-27 0.2761800472843774 1106 28-29 0.12181603658636675 1106 30-31 0.1153540696491433 1106 32-33 0.07780074962106909 1106 34-35 0.1655765594802645 1106 36-37 0.15889602811725467 1106 38-39 0.10401332032018473 1106 40-41 0.043353618840114905 1106 42-43 0.28640101434573495 1106 44-45 0.2307214713141903 1106 46-47 -0.5412849813146039 1106 48-49 -0.18128641591281536 1106 50-51 0.2851942572363697 1106 52-53 0.24851307687707447 1106 54-55 0.5115409358801699 1106 56-57 0.4389587902155583 1106 58-59 0.28799196805915983 1106 60-61 0.3791400159109841 1106 62-63 0.1796283007669075 1106 64-65 0.3111650490251634 1106 66-67 0.179495353548063 1106 68-69 0.05255704487096935 1106 70-71 0.07970170189703296 1106 72-73 0.4696141581027611 1106 74-75 0.6321804678354006 1106 76-77 0.31206925702155175 1106 78-79 0.5577375057314242 1106 80-81 0.05110095598615416 1106 82-83 0.45225762083491006 1106 84-85 0.5928837138195391 1106 86-87 0.8229781862250469 1106 88-89 1.1964928805428166 1106 90-91 1.1164816121384007 1106 92-93 0.9629129504442062 1106 94-95 1.2518299005748936 1106 96-97 1.3413552184522608 1106 98-99 1.3471886035117784 1106 100-101 1.145297419096913 1107 1 0.15093777814509934 1107 2 0.15187593721904946 1107 3 0.12511120220725047 1107 4 0.05163810278566672 1107 5 0.05721317545219051 1107 6 0.08317991424463145 1107 7 0.10845999520521588 1107 8 0.036382567211752814 1107 9 0.14417960030262122 1107 10-11 0.1384133440555999 1107 12-13 0.13934604920272875 1107 14-15 0.3273471368531844 1107 16-17 0.14592524850287703 1107 18-19 0.1491565159732815 1107 20-21 0.19901229260030107 1107 22-23 0.11483559020839351 1107 24-25 0.08260382855402071 1107 26-27 0.132423861698598 1107 28-29 0.19506799142455833 1107 30-31 0.12179990515696915 1107 32-33 0.0448123379746761 1107 34-35 0.14155648866806558 1107 36-37 0.11438270476544687 1107 38-39 0.15474477790623808 1107 40-41 0.21211474186114287 1107 42-43 0.14158121142387614 1107 44-45 0.16730452890509895 1107 46-47 0.148100001820886 1107 48-49 0.1130320304556065 1107 50-51 0.15455647851953813 1107 52-53 0.12137908426281285 1107 54-55 -0.04573083048591542 1107 56-57 0.01705632745595409 1107 58-59 0.1907356649679386 1107 60-61 0.09799928080135345 1107 62-63 0.32844770207391605 1107 64-65 0.22533808102967612 1107 66-67 0.1677976316089662 1107 68-69 0.3444907651815363 1107 70-71 0.4891680341053295 1107 72-73 0.5048903400254581 1107 74-75 0.27403243083828954 1107 76-77 0.3492197334978506 1107 78-79 0.6147004755187311 1107 80-81 0.632310456604575 1107 82-83 0.6160651722973256 1107 84-85 0.7903623669975346 1107 86-87 0.9316907182632086 1107 88-89 1.0276367325808486 1107 90-91 1.0217802249121846 1107 92-93 1.201892677721247 1107 94-95 1.136704633153471 1107 96-97 1.1130500055136423 1107 98-99 1.1751356731025844 1107 100-101 1.13925981845874 1108 1 0.1698457616007154 1108 2 0.1884662074421577 1108 3 0.20227651789469547 1108 4 0.14715178843693622 1108 5 0.08954438484443017 1108 6 0.1663743325601459 1108 7 0.13204569309299785 1108 8 0.12929735600366854 1108 9 0.20613010893062267 1108 10-11 0.24310878071771214 1108 12-13 0.3178110178071236 1108 14-15 0.39351621783287527 1108 16-17 0.43987051680380773 1108 18-19 0.40125101090335846 1108 20-21 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2316 70-71 -0.9912151870613108 2316 72-73 -1.085234173318078 2316 74-75 -1.1089261700867716 2316 76-77 -1.1485167420279048 2316 78-79 -1.3846571483498948 2316 80-81 -1.4684074136702812 2316 82-83 -1.4874522724650845 2316 84-85 -1.5241859055799978 2316 86-87 -1.6764052487273382 2316 88-89 -1.7314191850785434 2316 90-91 -1.5864945987268442 2316 92-93 -1.7145591703254723 2316 94-95 -1.4556548083978385 2316 96-97 -1.6364557990085196 2316 98-99 -1.6271151650279911 2316 100-101 -1.9795347917169543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 12.0 4 38.0 5 61.0 6 171.0 7 472.0 8 1060.0 9 1911.0 10 3136.0 11 4933.0 12 6727.0 13 9645.0 14 12618.0 15 16371.0 16 20523.0 17 24898.0 18 25010.0 19 23017.0 20 23551.0 21 25531.0 22 28129.0 23 31843.0 24 36965.0 25 43083.0 26 51237.0 27 60921.0 28 73159.0 29 86273.0 30 101853.0 31 120095.0 32 143545.0 33 174569.0 34 218402.0 35 279154.0 36 358653.0 37 387966.0 38 177154.0 39 6883.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22446721657829 19.341974580220082 14.835402544459027 23.598155658742595 2 16.180582974633616 20.326774215818677 39.06493984006479 24.427702969482922 3 19.676225734859422 23.610904897124527 29.485787387457236 27.227081980558815 4 13.624686237073934 16.547764651153777 35.84582731651532 33.981721795256966 5 14.08927790918783 37.13229262776024 34.13660879841051 14.641820664641415 6 33.521503938247164 35.67247925432649 16.62072884902701 14.185287958399332 7 29.643526549916167 31.888237403448244 20.00449686479362 18.46373918184197 8 26.640745083977013 35.732591609082895 18.971691073141894 18.654972233798205 9 26.21811732552843 16.64173361891009 19.626488859598965 37.51366019596251 10-11 25.39532674956146 26.448949923921578 26.934785768973573 21.220937557543394 12-13 26.100830563174167 23.37761065291763 27.414089530252078 23.107469253656127 14-15 22.756171074692535 25.02617679268872 25.461035248054426 26.756616884564316 16-17 22.714575075351558 28.292974617911863 26.19532287295388 22.797127433782695 18-19 22.681817741294594 27.039822451372803 28.121919308413208 22.156440498919398 20-21 23.114970489324794 26.180533615033486 28.414777628097653 22.289718267544075 22-23 22.981188761714623 26.034618105695706 28.210305961253308 22.773887171336362 24-25 22.90502121888864 26.19281013761408 28.268971729535693 22.63319691396159 26-27 22.507906952943234 26.562516051836504 28.179358787160545 22.750218208059714 28-29 22.574444462535286 26.74581151771572 28.150558968263752 22.529185051485246 30-31 22.671198491200933 26.392295510624976 28.07050495535048 22.866001042823612 32-33 22.49686139124539 26.371834182216276 28.05017819854615 23.081126227992186 34-35 23.087726576183197 26.489357062062563 27.949796310422336 22.473120051331904 36-37 23.118417886887837 26.1515626269263 27.908440711568584 22.82157877461728 38-39 22.728007548819992 26.501448356177765 27.92642769306634 22.844116401935896 40-41 23.08332997357681 26.21749823225118 27.94492997233628 22.75424182183573 42-43 22.81368946583807 26.2743766295264 27.82757660215724 23.08435730247829 44-45 22.77898887987293 26.46547984261316 27.92143219079646 22.83409908671745 46-47 23.083104775011385 26.675731467392882 27.36933409573282 22.871829661862904 48-49 22.956882432183594 26.603316437785296 27.445567583807414 22.9942335462237 50-51 23.055677669309752 26.839362651488898 27.27507615633972 22.829883522861632 52-53 23.250826201990638 26.666001182365314 27.22653925549751 22.856633360146535 54-55 22.927128811438326 26.748297563362122 27.336503668486372 22.98806995671318 56-57 22.926567360902474 26.8569822548288 27.326846476648285 22.889603907620444 58-59 23.017250738652667 26.601112050313684 27.181157959224823 23.200479251808826 60-61 22.754872411153 26.77326691412345 27.390151478441215 23.081709196282333 62-63 22.77287258747733 26.781996577437607 27.430298439066526 23.01483239601854 64-65 22.570187434015416 26.674578677660254 27.372023351417745 23.383210536906585 66-67 22.56510964876573 26.727812242916816 27.29342433060481 23.413653777712646 68-69 22.82632675382904 26.56896343825206 27.360186664629836 23.244523143289065 70-71 22.73024978741641 26.56173588385832 27.194437375015678 23.513576953709595 72-73 22.757588708981817 26.65416459510248 27.039053951769137 23.54919274414656 74-75 22.56291457235772 26.932066647201857 26.9891427456938 23.515876034746626 76-77 22.73200808445 26.575088925459305 27.153826790930243 23.53907619916045 78-79 22.76329871801832 26.65065805290792 27.171962370386453 23.414080858687306 80-81 22.700215904666923 26.806689322180326 27.040140120888 23.452954652264747 82-83 22.719404004918797 26.738651844607258 27.066339005358948 23.475605145115 84-85 22.686981447153798 26.720658133440452 26.988437766679475 23.60392265272628 86-87 22.730579595381382 26.765289991538545 26.868165045937094 23.635965367142983 88-89 22.687761527582975 26.845024355892843 26.735592582461816 23.73162153406236 90-91 22.598080965787094 26.72269973072411 26.89267177558649 23.786547527902304 92-93 22.56272782932959 26.567774051468053 26.988176237001056 23.881321882201306 94-95 22.443672026287036 26.496657120012536 26.98934512744512 24.070325726255316 96-97 22.527084296382245 26.293038591244294 26.97425673306921 24.205620379304246 98-99 22.374943275037527 26.398284869852574 26.727037541258923 24.499734313850976 100-101 22.50172726504349 26.337845288230326 26.93825890132761 24.222168545398574 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 268.0 1 198.5 2 175.0 3 233.0 4 201.0 5 199.0 6 384.0 7 854.0 8 1381.5 9 1592.5 10 1405.5 11 1223.0 12 1298.0 13 1414.0 14 1541.5 15 1736.0 16 1950.0 17 2108.0 18 2272.0 19 2558.5 20 2934.0 21 3444.0 22 4135.0 23 5078.5 24 6362.5 25 8144.5 26 10776.5 27 14028.0 28 17205.0 29 20782.0 30 24988.5 31 30041.5 32 36948.5 33 41560.0 34 43972.0 35 48055.0 36 53836.0 37 59232.5 38 63316.0 39 67752.5 40 72135.5 41 77260.0 42 81483.0 43 85608.0 44 90598.5 45 95033.0 46 97580.0 47 101137.5 48 104380.5 49 103585.5 50 102370.0 51 99988.5 52 96380.0 53 94869.0 54 94739.0 55 89585.0 56 81751.5 57 74804.5 58 68135.0 59 60441.0 60 49815.0 61 40350.5 62 33118.5 63 25473.0 64 18988.5 65 14329.5 66 10450.0 67 7954.0 68 5986.5 69 4315.0 70 3195.0 71 2254.0 72 1638.0 73 1332.5 74 1031.0 75 750.0 76 501.5 77 305.5 78 196.5 79 127.0 80 75.0 81 41.0 82 16.5 83 4.0 84 3.5 85 3.0 86 1.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.488946822635512E-4 2 0.04965934310884545 3 0.0 4 0.0 5 0.04388319781359332 6 3.876607580706124E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005873060484769778 26-27 0.0016087921459930416 28-29 3.876607580706124E-5 30-31 0.0015506430322824497 32-33 3.295116443600205E-4 34-35 0.0109514164154948 36-37 0.022038514096314316 38-39 0.003837841504899063 40-41 8.916197435624085E-4 42-43 0.0011435992363083066 44-45 0.007675683009798126 46-47 0.0 48-49 0.0 50-51 5.039589854917961E-4 52-53 0.0 54-55 0.001434344804861266 56-57 0.0 58-59 0.02151517207291899 60-61 0.0 62-63 0.008567302753360534 64-65 0.014498512351840905 66-67 0.006086273901708615 68-69 0.015661494626052742 70-71 5.814911371059186E-5 72-73 9.69151895176531E-5 74-75 0.021786534603568417 76-77 0.050085769942723124 78-79 0.029268387234331235 80-81 0.025546843956853357 82-83 0.03473440392312687 84-85 0.01558396247443862 86-87 0.009013112625141739 88-89 0.08412238450132288 90-91 0.02409311611408856 92-93 0.023879902697149725 94-95 0.02562437610846748 96-97 0.03717666669897173 98-99 0.052140371960497374 100-101 0.013684424759892618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2579575.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.154908844642435 #Duplication Level Percentage of deduplicated Percentage of total 1 75.54108653765128 42.420028285470536 2 13.786099751670719 15.483143497564338 3 4.64658998108808 7.827865104792899 4 2.0486713826593723 4.601718189834585 5 1.08482811006164 3.045921181630856 6 0.6504613829223889 2.191595979898008 7 0.4556317325840317 1.7910170886987962 8 0.3073293202841352 1.3806439972673223 9 0.2142271951791897 1.0826917755597738 >10 1.1550191841053452 11.53033237827049 >50 0.07268357045849706 2.7744253690445144 >100 0.03357413080182608 3.5044708486635803 >500 0.002623879641401028 1.0341202810317636 >1k 0.0011047914279465436 1.1091104806299859 >5k 6.904946424665897E-5 0.22291554164249017 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5749 0.22286616981479507 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4739 0.18371243324966322 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3302 0.1280055823149162 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.876607580706124E-5 2 0.0 0.0 0.0 0.0 3.876607580706124E-5 3 0.0 0.0 0.0 0.0 3.876607580706124E-5 4 0.0 0.0 0.0 0.0 3.876607580706124E-5 5 0.0 0.0 0.0 0.0 3.876607580706124E-5 6 0.0 0.0 0.0 0.0 3.876607580706124E-5 7 0.0 0.0 0.0 0.0 3.876607580706124E-5 8 0.0 0.0 0.0 0.0 7.753215161412248E-5 9 0.0 0.0 0.0 0.0 7.753215161412248E-5 10-11 0.0 0.0 0.0 0.0 7.753215161412248E-5 12-13 0.0 0.0 0.0 0.0 1.938303790353062E-4 14-15 0.0 0.0 0.0 0.0 2.1321341693883683E-4 16-17 0.0 0.0 0.0 3.876607580706124E-5 2.3259645484236744E-4 18-19 0.0 0.0 0.0 3.876607580706124E-5 2.3259645484236744E-4 20-21 0.0 0.0 0.0 3.876607580706124E-5 2.5197949274589806E-4 22-23 0.0 0.0 0.0 1.744473411317756E-4 2.713625306494287E-4 24-25 0.0 0.0 0.0 3.295116443600205E-4 3.295116443600205E-4 26-27 0.0 0.0 0.0 7.559384782376942E-4 3.4889468226355114E-4 28-29 0.0 0.0 0.0 0.0029462217613366545 3.4889468226355114E-4 30-31 0.0 0.0 0.0 0.010641287809038311 3.4889468226355114E-4 32-33 0.0 0.0 0.0 0.026128335093959277 3.876607580706124E-4 34-35 0.0 0.0 0.0 0.05262494790808564 3.876607580706124E-4 36-37 0.0 0.0 0.0 0.09865966292897085 3.876607580706124E-4 38-39 0.0 0.0 0.0 0.17031875405832358 4.0704379597414304E-4 40-41 0.0 0.0 0.0 0.24562185631354 4.2642683387767366E-4 42-43 0.0 0.0 0.0 0.32067297907601056 4.2642683387767366E-4 44-45 0.0 0.0 0.0 0.4136727949371505 4.2642683387767366E-4 46-47 0.0 0.0 0.0 0.5268115871800587 4.458098717812043E-4 48-49 0.0 0.0 0.0 0.6544488917748079 4.845759475882655E-4 50-51 0.0 0.0 0.0 0.7870288710349573 5.039589854917961E-4 52-53 1.938303790353062E-5 0.0 0.0 0.917864376883789 5.814911371059186E-4 54-55 3.876607580706124E-5 0.0 0.0 1.0610856439529768 6.202572129129798E-4 56-57 3.876607580706124E-5 0.0 0.0 1.2350096430613569 6.396402508165105E-4 58-59 3.876607580706124E-5 0.0 0.0 1.4238198152796488 6.59023288720041E-4 60-61 3.876607580706124E-5 0.0 0.0 1.6273223302288167 6.977893645271023E-4 62-63 3.876607580706124E-5 0.0 0.0 1.827587877848095 7.17172402430633E-4 64-65 3.876607580706124E-5 0.0 0.0 2.0415572332651695 7.365554403341636E-4 66-67 3.876607580706124E-5 0.0 0.0 2.2851632536367426 7.365554403341636E-4 68-69 3.876607580706124E-5 0.0 0.0 2.5512535979764106 7.365554403341636E-4 70-71 3.876607580706124E-5 0.0 0.0 2.843840555130206 7.753215161412249E-4 72-73 3.876607580706124E-5 0.0 0.0 3.143056511247008 8.528536677553473E-4 74-75 3.876607580706124E-5 0.0 0.0 3.447021311650175 9.110027814659392E-4 76-77 3.876607580706124E-5 0.0 0.0 3.7776571722086003 9.303858193694698E-4 78-79 3.876607580706124E-5 0.0 0.0 4.158146206254907 9.69151895176531E-4 80-81 3.876607580706124E-5 0.0 0.0 4.560712520473334 9.69151895176531E-4 82-83 3.876607580706124E-5 0.0 0.0 4.974365932372581 0.0010079179709835922 84-85 3.876607580706124E-5 0.0 0.0 5.3891047943944255 0.0010079179709835922 86-87 3.876607580706124E-5 0.0 0.0 5.820067259141525 0.0011048331605012455 88-89 3.876607580706124E-5 0.0 0.0 6.280026748592307 0.0011823653121153677 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4605 0.0 45.409477 1 GTATCAA 7265 0.0 35.32495 1 ATCAACG 9190 0.0 27.550186 3 TCAACGC 9290 0.0 27.304762 4 TATCAAC 9525 0.0 26.843597 2 CAACGCA 9600 0.0 26.472527 5 AACGCAG 10040 0.0 25.395676 6 GTACATG 9130 0.0 22.435198 1 ACGCAGA 11595 0.0 21.948923 7 TACATGG 9065 0.0 21.862091 2 CGCAGAG 11755 0.0 21.569386 8 ACATGGG 8970 0.0 20.970827 3 CTATGCG 165 6.324463E-5 20.152407 4 AGAGTAC 11095 0.0 19.129314 10-11 GGACCGT 250 2.1838423E-7 18.99237 6 GCAGAGT 13120 0.0 18.927229 9 GAGTACT 6775 0.0 18.43171 12-13 GAGTACA 7105 0.0 18.394672 1 AGTACTT 7175 0.0 17.933563 12-13 GTACTTT 7060 0.0 17.586773 14-15 >>END_MODULE