FastQCFastQC Report
Thu 26 May 2016
SRR938438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2598795
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56150.21606167473771498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45910.1766587976350578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30820.11859342502967721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44900.044.309271
GTATCAA72650.036.62191
ATCAACG92150.028.5738013
TATCAAC93700.028.4049262
TCAACGC94000.028.1631364
CAACGCA95150.027.771735
AACGCAG98100.027.1241136
GTACATG89350.024.2904191
TACATGG87100.024.0638832
ACGCAGA113600.023.2932347
CGCAGAG116650.022.562028
ACATGGG92200.021.963
GAGTACT64750.020.3932712-13
TAAGGTG12700.020.2081685
AGAGTAC107350.019.8684410-11
GCAGAGT127900.019.7595629
GTACTTT67800.019.54875414-15
TAGCCCT16100.019.1885034
AGTACTT69450.018.73959412-13
GAGTACA68500.018.4128061
Produced by FastQC (version 0.11.5)