FastQCFastQC Report
Thu 26 May 2016
SRR938438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2598795
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57340.22064072002601207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.1831233321597125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.13367733892053818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47650.041.6245541
GTATCAA77450.037.829941
ATCAACG97850.029.7817483
TCAACGC99400.029.2695224
TATCAAC99700.029.1489222
CAACGCA100750.028.8773215
TAGCGCG1703.614332E-927.964154
AACGCAG105550.027.5836376
ACGCAGA120450.024.1714637
CGCAGAG120650.023.8951978
CGCGCGT2052.6755515E-823.1683657
GTACATG89900.021.5862351
TACATGG88900.021.240522
GCAGAGT134250.020.9792359
AGAGTAC116500.020.15995810-11
ACATGGG89150.020.1567733
AGTACTT75800.019.54945612-13
GAGTACT71350.019.43739912-13
GTACTTT74550.018.4437914-15
GGACCGA10600.018.3707666
Produced by FastQC (version 0.11.5)