##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938438_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2598795 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74993756721865 31.0 31.0 34.0 30.0 34.0 2 31.97349617803636 31.0 31.0 34.0 30.0 34.0 3 32.04399038785283 33.0 31.0 34.0 30.0 34.0 4 35.724153309514605 37.0 35.0 37.0 33.0 37.0 5 35.584080314145595 37.0 35.0 37.0 33.0 37.0 6 35.61204058034589 37.0 35.0 37.0 33.0 37.0 7 35.54258146564081 37.0 35.0 37.0 33.0 37.0 8 35.53167448759906 37.0 35.0 37.0 33.0 37.0 9 37.10205960839543 39.0 37.0 39.0 33.0 39.0 10-11 37.00907920786365 39.0 37.0 39.0 33.0 39.0 12-13 36.90003347705379 39.0 37.0 39.0 33.0 39.0 14-15 37.95173820943938 40.0 37.0 41.0 33.0 41.0 16-17 37.87074105498895 40.0 37.0 41.0 33.0 41.0 18-19 37.967629420558374 40.0 37.0 41.0 33.0 41.0 20-21 38.017212785156204 40.0 37.0 41.0 33.0 41.0 22-23 37.91641183702447 40.0 37.0 41.0 32.5 41.0 24-25 37.832128928984396 40.0 37.0 41.0 32.0 41.0 26-27 37.64191808126459 40.0 37.0 41.0 32.0 41.0 28-29 37.52130679795828 39.5 36.5 41.0 32.0 41.0 30-31 37.296008149931026 39.0 36.0 41.0 31.0 41.0 32-33 37.09913786966652 39.0 36.0 41.0 31.0 41.0 34-35 36.9611389124575 39.0 36.0 40.5 30.5 41.0 36-37 36.97950954192231 39.0 36.0 41.0 30.5 41.0 38-39 36.86860641181779 39.0 35.5 41.0 30.0 41.0 40-41 36.7031449190875 39.0 35.0 41.0 30.0 41.0 42-43 36.501347932407135 39.0 35.0 40.0 30.0 41.0 44-45 36.262118597272966 39.0 35.0 40.0 30.0 41.0 46-47 35.927806925902196 38.0 35.0 40.0 28.5 41.0 48-49 35.60208385039989 38.0 34.5 40.0 28.0 41.0 50-51 35.247261519281054 38.0 34.0 40.0 27.0 41.0 52-53 34.7580082307377 38.0 33.0 40.0 26.0 41.0 54-55 34.44765901119557 38.0 33.0 40.0 25.0 41.0 56-57 34.11321804913431 37.0 33.0 40.0 24.0 41.0 58-59 33.66801690783613 37.0 32.0 39.5 23.0 41.0 60-61 33.295264536063826 36.0 32.0 39.0 22.5 40.5 62-63 32.83228746399774 36.0 31.0 39.0 22.0 40.0 64-65 32.3209853028038 35.0 31.0 38.5 20.5 40.0 66-67 31.826962111286193 35.0 30.0 38.0 18.5 40.0 68-69 31.279095311480898 34.5 29.5 37.5 17.5 40.0 70-71 30.70102643725265 34.0 29.0 37.0 14.0 39.0 72-73 30.250922061955634 34.0 29.0 36.0 12.0 39.0 74-75 29.71318110893703 34.0 28.5 36.0 8.0 39.0 76-77 28.464022556607965 31.5 26.5 34.5 7.0 36.5 78-79 29.166059077380094 33.5 28.5 35.0 7.0 37.0 80-81 28.887155008378883 33.0 28.0 35.0 2.0 37.0 82-83 28.402034404406656 33.0 27.0 35.0 2.0 36.0 84-85 27.866095632783654 33.0 26.0 35.0 2.0 36.0 86-87 27.283123524556572 32.5 25.5 35.0 2.0 35.5 88-89 26.626342016203665 32.0 25.0 34.0 2.0 35.0 90-91 26.07672652133008 32.0 24.0 34.0 2.0 35.0 92-93 25.31762355245412 31.0 21.0 34.0 2.0 35.0 94-95 24.527489471081793 31.0 18.5 34.0 2.0 35.0 96-97 23.50845103211296 31.0 4.5 34.0 2.0 35.0 98-99 22.244934479249036 30.0 2.0 34.0 2.0 35.0 100-101 18.5930206114757 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2391413030585845 1101 2 0.26197661200560773 1101 3 0.15004090813707194 1101 4 0.1975043140310575 1101 5 0.06533642635282888 1101 6 0.18664825734655466 1101 7 0.21450881196937388 1101 8 0.1949350978056401 1101 9 0.1967740188026994 1101 10-11 0.28799839670116256 1101 12-13 0.2829401054526173 1101 14-15 0.3952318485603499 1101 16-17 0.32510582952551204 1101 18-19 0.39273628438498065 1101 20-21 0.4176027056106122 1101 22-23 0.4226440742694635 1101 24-25 0.4935722036695793 1101 26-27 0.5346906754415457 1101 28-29 0.4939829955143509 1101 30-31 0.4443568753571512 1101 32-33 0.3587319280765229 1101 34-35 0.3751886938465461 1101 36-37 0.3227608696609039 1101 38-39 0.37671625280699317 1101 40-41 0.5333753397069074 1101 42-43 0.6768649220737117 1101 44-45 0.70675586970642 1101 46-47 0.6634707441080607 1101 48-49 0.6903438961410089 1101 50-51 0.8081069391669473 1101 52-53 0.6681138367683062 1101 54-55 0.712140213212983 1101 56-57 0.680438648909238 1101 58-59 0.6918534177601501 1101 60-61 0.6223189127521565 1101 62-63 0.6305023610050284 1101 64-65 0.5662768947783832 1101 66-67 0.44339130126105175 1101 68-69 0.5242804411109958 1101 70-71 0.44387758831670965 1101 72-73 0.27151348243558004 1101 74-75 0.3271475554903418 1101 76-77 0.4678504809306112 1101 78-79 0.505297793717201 1101 80-81 0.43588183471886666 1101 82-83 0.486116858101699 1101 84-85 0.6013449746365858 1101 86-87 0.5683957786837475 1101 88-89 0.5650865929529658 1101 90-91 0.605795004364964 1101 92-93 0.565237014579445 1101 94-95 0.5143734835349854 1101 96-97 0.7196453926823345 1101 98-99 0.49821887886674787 1101 100-101 0.33893428974574746 1102 1 0.3337124438230781 1102 2 0.22030210013424423 1102 3 0.2726873467279489 1102 4 0.23793110213252788 1102 5 0.15927277798297013 1102 6 0.15797717226060115 1102 7 0.17827169939920395 1102 8 0.20228627875550842 1102 9 0.24044704053346777 1102 10-11 0.2990688771235668 1102 12-13 0.3314387686297451 1102 14-15 0.2747125251487361 1102 16-17 0.2942477815558959 1102 18-19 0.33658131783244727 1102 20-21 0.25901452640641764 1102 22-23 0.248249544753115 1102 24-25 0.28230274644118225 1102 26-27 0.24342349780548744 1102 28-29 0.32765916429294606 1102 30-31 0.18306768183523303 1102 32-33 0.21507171644427814 1102 34-35 0.26754015722378455 1102 36-37 0.2896852625093018 1102 38-39 0.3830587645815271 1102 40-41 0.4246241595639759 1102 42-43 0.34262742889904274 1102 44-45 0.42270185345474687 1102 46-47 0.3674650067977936 1102 48-49 0.5092255779018515 1102 50-51 0.34291308640037244 1102 52-53 0.3745144478873357 1102 54-55 0.4423565122309441 1102 56-57 0.4379011196914533 1102 58-59 0.4241178195386439 1102 60-61 0.3982477625734262 1102 62-63 0.4201669314330019 1102 64-65 0.40513375655305595 1102 66-67 0.2950049702077564 1102 68-69 0.21142320411139437 1102 70-71 0.10095763899224863 1102 72-73 -0.14268373794115163 1102 74-75 -0.17209745563191703 1102 76-77 -0.09790230904832598 1102 78-79 -0.05557757646439043 1102 80-81 -0.09373723128037525 1102 82-83 -0.19237942461616697 1102 84-85 -0.1281208745149236 1102 86-87 -0.21880881573709843 1102 88-89 0.02802674255877946 1102 90-91 0.0010941352956024275 1102 92-93 0.07606575062282772 1102 94-95 -0.027647674309637438 1102 96-97 -0.12085122894837141 1102 98-99 -0.36719910019058943 1102 100-101 -0.5323153101488138 1103 1 0.33320653189919724 1103 2 0.26788667527082666 1103 3 0.31715437589001994 1103 4 0.1797646962231667 1103 5 0.18527049528785255 1103 6 0.10154971836335136 1103 7 0.24000898427442507 1103 8 0.18092897284871157 1103 9 0.32776974693783956 1103 10-11 0.2733193067319135 1103 12-13 0.24586623535112295 1103 14-15 0.448526613737819 1103 16-17 0.4790207303363161 1103 18-19 0.40214852909716825 1103 20-21 0.3882589927943698 1103 22-23 0.42166063506063267 1103 24-25 0.37330148967645727 1103 26-27 0.3685308998932513 1103 28-29 0.33760299460406173 1103 30-31 0.3992584664154961 1103 32-33 0.5027467658171787 1103 34-35 0.3497902790530176 1103 36-37 0.47767546487407486 1103 38-39 0.4467036387768104 1103 40-41 0.5418056081776825 1103 42-43 0.5147910519722174 1103 44-45 0.5833292098886105 1103 46-47 0.5113536677021528 1103 48-49 0.591680885809204 1103 50-51 0.5619408891139557 1103 52-53 0.5476940626504785 1103 54-55 0.4530216998479659 1103 56-57 0.6894669459501372 1103 58-59 0.6163217103796441 1103 60-61 0.5122029807118693 1103 62-63 0.6059552306968925 1103 64-65 0.41772929632067246 1103 66-67 0.29898942736234346 1103 68-69 0.3254438253122487 1103 70-71 0.21036734429374349 1103 72-73 0.32655438050251107 1103 74-75 0.2768557747663998 1103 76-77 0.17452388808419883 1103 78-79 0.33005266539869993 1103 80-81 0.29532090050038207 1103 82-83 0.2761857476118941 1103 84-85 0.24385263083273756 1103 86-87 0.3394797400093914 1103 88-89 0.352936973649598 1103 90-91 0.48441763679602445 1103 92-93 0.22419086283396084 1103 94-95 0.29522031409734595 1103 96-97 0.2490149512563029 1103 98-99 0.12001075387081173 1103 100-101 0.010065553684572848 1104 1 0.32534137365530924 1104 2 0.31072614675271737 1104 3 0.2834350793591085 1104 4 0.23338436110407912 1104 5 0.15715860973902096 1104 6 0.15763665629362578 1104 7 0.21788874212858644 1104 8 0.28901740128313236 1104 9 0.3925562810881047 1104 10-11 0.3755434565642375 1104 12-13 0.4104395785397017 1104 14-15 0.6316406983911307 1104 16-17 0.47497315637616566 1104 18-19 0.5374336185579551 1104 20-21 0.5167922206521425 1104 22-23 0.5395173634040376 1104 24-25 0.5450834667935496 1104 26-27 0.5579081562660733 1104 28-29 0.5074607553048409 1104 30-31 0.4818882899373662 1104 32-33 0.5762124322985542 1104 34-35 0.5391777673702762 1104 36-37 0.6216535837845427 1104 38-39 0.583797033522174 1104 40-41 0.6242860758539024 1104 42-43 0.634271299737982 1104 44-45 0.6654297211804021 1104 46-47 0.6125711163744398 1104 48-49 0.48879746278091574 1104 50-51 0.5195281049866054 1104 52-53 0.5941794660945163 1104 54-55 0.6160003868604207 1104 56-57 0.45337185300743243 1104 58-59 0.5456828094431998 1104 60-61 0.43857268682598516 1104 62-63 0.40738052528526225 1104 64-65 0.5937863936249386 1104 66-67 0.5284533040170132 1104 68-69 0.3095691925932371 1104 70-71 0.14642883980695842 1104 72-73 0.005827973533794051 1104 74-75 0.11071511149205904 1104 76-77 0.09559130896225199 1104 78-79 0.11341231671847041 1104 80-81 0.19011299349806876 1104 82-83 0.08730848207613917 1104 84-85 0.23985148241151322 1104 86-87 0.270189012289066 1104 88-89 0.24809482048197395 1104 90-91 0.1297343078405362 1104 92-93 0.16442332650292357 1104 94-95 0.33609097621105377 1104 96-97 0.2525423759217027 1104 98-99 -0.01925685270939681 1104 100-101 -0.12474123055273267 1105 1 0.2940360967995552 1105 2 0.3404343515835002 1105 3 0.25719101152714785 1105 4 0.20132385719062995 1105 5 0.17704364836365727 1105 6 0.20702624626935773 1105 7 0.23327196699158037 1105 8 0.2536263083852788 1105 9 0.34021733680688016 1105 10-11 0.29840354517522627 1105 12-13 0.3297689009906648 1105 14-15 0.5332756331611819 1105 16-17 0.40740423430273154 1105 18-19 0.4224264718561912 1105 20-21 0.4206328703501967 1105 22-23 0.44189550301356206 1105 24-25 0.44141834148148007 1105 26-27 0.48397064514914945 1105 28-29 0.4069033009791312 1105 30-31 0.4988979905638544 1105 32-33 0.45603515237902315 1105 34-35 0.5173765609768921 1105 36-37 0.4442867566478199 1105 38-39 0.45478994128249894 1105 40-41 0.5895983551448083 1105 42-43 0.5648064883361741 1105 44-45 0.5125812666003426 1105 46-47 0.37089657491343786 1105 48-49 0.4442690494993471 1105 50-51 0.38804892602010455 1105 52-53 0.4879365164736953 1105 54-55 0.4829335508591157 1105 56-57 0.5104140918399551 1105 58-59 0.4352087632396362 1105 60-61 0.39292693148015445 1105 62-63 0.4786829979224052 1105 64-65 0.48613047124849373 1105 66-67 0.5714628621150339 1105 68-69 0.5679301384028719 1105 70-71 0.2821115946157029 1105 72-73 0.1541662132840571 1105 74-75 0.3576322091721451 1105 76-77 0.28811641511988384 1105 78-79 0.32438428554065624 1105 80-81 0.25546215058124133 1105 82-83 0.3025729621861757 1105 84-85 0.4382684894180038 1105 86-87 0.5229616544713522 1105 88-89 0.48299216232607023 1105 90-91 0.4516500584528025 1105 92-93 0.39824909761958693 1105 94-95 0.384017042176243 1105 96-97 0.5068166366132196 1105 98-99 0.2963953222112714 1105 100-101 -0.02751552397944934 1106 1 0.08697099921728935 1106 2 0.1307697034465214 1106 3 0.15845026812036167 1106 4 0.052831174735644026 1106 5 -0.0225489715558993 1106 6 -0.015794461505734603 1106 7 0.1403635521522375 1106 8 0.08398489733405512 1106 9 -0.00612883953402843 1106 10-11 -0.05046668368644447 1106 12-13 0.029566785583547528 1106 14-15 0.15292876960538848 1106 16-17 0.03812802565934703 1106 18-19 0.24685698892071173 1106 20-21 0.2275860846262603 1106 22-23 0.18363767708700607 1106 24-25 0.251740145876056 1106 26-27 0.12308751216912839 1106 28-29 0.3569794176189518 1106 30-31 0.29333427753398666 1106 32-33 0.1644537314750849 1106 34-35 0.039776701119237146 1106 36-37 -0.31501468549117106 1106 38-39 -0.03460476412614355 1106 40-41 -0.04501253051883225 1106 42-43 0.18271599979500053 1106 44-45 0.18922642077941987 1106 46-47 -0.14134057383090948 1106 48-49 -0.1954354930922264 1106 50-51 -0.08607015110992222 1106 52-53 -0.18727178499206332 1106 54-55 -0.4043969511339185 1106 56-57 -0.2149180151541188 1106 58-59 -0.08920522017722732 1106 60-61 -0.41403148470886464 1106 62-63 -0.27191184785353073 1106 64-65 -0.7698943280982569 1106 66-67 -0.52387723614207 1106 68-69 -1.0933921485042788 1106 70-71 -0.8714402996679311 1106 72-73 -0.7949043876409263 1106 74-75 -0.47855434998457014 1106 76-77 -0.3801450830760338 1106 78-79 -0.4769767317173219 1106 80-81 -1.0789480255977075 1106 82-83 -0.7426828369626222 1106 84-85 -0.5062022145329763 1106 86-87 -0.33170420838260384 1106 88-89 -0.23453960589693423 1106 90-91 -0.3575621919678511 1106 92-93 -0.6360794507036509 1106 94-95 -0.678272772421959 1106 96-97 -0.7851691275787225 1106 98-99 -0.9911744396592148 1106 100-101 -1.0408786759900295 1107 1 0.13786262475342426 1107 2 0.17302027999241787 1107 3 0.15849014138802886 1107 4 0.24584163338598586 1107 5 0.06656724285451077 1107 6 0.13664050196173605 1107 7 0.10602323920096524 1107 8 0.14174482793301735 1107 9 0.1342288344535092 1107 10-11 0.1983323361855227 1107 12-13 0.21815575685324262 1107 14-15 0.346717632570666 1107 16-17 0.23299084006545456 1107 18-19 0.22199661271104532 1107 20-21 0.26018685892201887 1107 22-23 0.232806804401271 1107 24-25 0.22619438052485208 1107 26-27 0.2686129502362462 1107 28-29 0.3051135238111655 1107 30-31 0.24587855572007555 1107 32-33 0.21215825709096947 1107 34-35 0.17755076248096202 1107 36-37 0.14770913016501908 1107 38-39 0.12773486720778493 1107 40-41 0.23005374531796718 1107 42-43 0.20349381139920553 1107 44-45 0.17257744563016075 1107 46-47 -0.025188667457655356 1107 48-49 0.029770204474424133 1107 50-51 -0.034516508094213805 1107 52-53 0.24647985843316178 1107 54-55 0.17606622536456484 1107 56-57 0.07682168514977405 1107 58-59 -0.10807153965908611 1107 60-61 -0.2056184946448596 1107 62-63 -0.05571528611461929 1107 64-65 0.14424916447669034 1107 66-67 0.1231562466262126 1107 68-69 0.12182058076700741 1107 70-71 -0.1213107789014991 1107 72-73 -0.01380276738530739 1107 74-75 0.06754675838450552 1107 76-77 0.02797000248984105 1107 78-79 0.06953860063175199 1107 80-81 0.26580742734105556 1107 82-83 0.23124152775923434 1107 84-85 0.27315481555517707 1107 86-87 0.19388580821329882 1107 88-89 0.08154366726727957 1107 90-91 0.08114794823518068 1107 92-93 0.05770860401971234 1107 94-95 0.11860290111296834 1107 96-97 0.11048650478974764 1107 98-99 0.08752198737270689 1107 100-101 -0.29406900887792986 1108 1 0.11206832636867503 1108 2 0.13185117742286323 1108 3 0.11880907078990077 1108 4 0.14487719244450403 1108 5 0.11571285087092065 1108 6 0.10131364184000802 1108 7 0.10250232662856718 1108 8 0.0670940248863161 1108 9 0.13576396018403614 1108 10-11 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-0.5406927385129379 2316 62-63 -0.492310390039421 2316 64-65 -0.48322991858453435 2316 66-67 -0.560556374991144 2316 68-69 -0.38746608627035073 2316 70-71 -0.5829880582262774 2316 72-73 -0.5594731799591841 2316 74-75 -0.23043022163838955 2316 76-77 -0.07631232169826418 2316 78-79 -0.1798023024891542 2316 80-81 -0.2647901346555912 2316 82-83 -0.042783695918274134 2316 84-85 -0.15825596813258258 2316 86-87 -0.0011687761929621843 2316 88-89 0.21037839676752768 2316 90-91 0.13625975982646565 2316 92-93 -0.06523020430756432 2316 94-95 -0.023117787530626543 2316 96-97 0.26229289360354 2316 98-99 0.3805571948100699 2316 100-101 0.587778839925722 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 38.0 5 70.0 6 178.0 7 428.0 8 1074.0 9 1877.0 10 3029.0 11 4764.0 12 6753.0 13 9375.0 14 12216.0 15 15872.0 16 19444.0 17 23803.0 18 23378.0 19 21375.0 20 21651.0 21 23349.0 22 26167.0 23 29394.0 24 34200.0 25 40022.0 26 47472.0 27 57609.0 28 69645.0 29 82756.0 30 98090.0 31 117082.0 32 140566.0 33 171280.0 34 216315.0 35 279190.0 36 368047.0 37 415353.0 38 207581.0 39 9336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25582773455236 19.37501442984785 14.81452835561302 23.55462947998676 2 16.181769744156753 20.283400217437503 39.16481362279829 24.370016415607452 3 19.663109855816405 23.590775636166338 29.511653589967562 27.23446091804969 4 13.602650459155107 16.563599668307813 35.888940836041314 33.94480903649576 5 14.065300664514524 37.10470552077773 34.136188889428084 14.693804925279666 6 33.48740473950427 35.718130902976185 16.610852337333267 14.18361202018628 7 29.641545408545117 31.836832070247944 20.040826613872966 18.480795907333977 8 26.626301805259743 35.758072491289234 18.97821875138285 18.63740695206817 9 26.165434364772906 16.65691214582143 19.69008713653828 37.48756635286738 10-11 25.350518220944707 26.481273051548893 26.934560055718133 21.23364867178827 12-13 26.086897966172785 23.419450168251053 27.412396899332194 23.08125496624397 14-15 22.742809648317778 25.047396966671094 25.474210162786985 26.735583222224147 16-17 22.720299215597997 28.306368913284814 26.20660729299541 22.76672457812178 18-19 22.685436904411468 27.027911012603916 28.127208956458666 22.15944312652595 20-21 23.125987236392252 26.15945082240038 28.444952372157097 22.26960956905027 22-23 22.939246843248505 26.047168014406672 28.207784761783827 22.805800380560992 24-25 22.91045784052329 26.23181507372478 28.22951630895491 22.62821077679702 26-27 22.475995973946485 26.58826983794384 28.172426844025445 22.763307344084225 28-29 22.59930082980766 26.781008121071498 28.126266211840488 22.493424837280354 30-31 22.681261892530962 26.387210226277947 28.11385661431548 22.81767126687561 32-33 22.493597994455122 26.4134146787261 28.02822077154989 23.064766555268886 34-35 23.088166052893822 26.51927780367902 27.928758607459443 22.463797535967718 36-37 23.115739928397502 26.146532696729864 27.92816816428556 22.80955921058707 38-39 22.652196583285072 26.549575844799023 27.914838024469972 22.88338954744593 40-41 23.085659818144947 26.24080151985848 27.92353221637434 22.75000644562223 42-43 22.7644561729977 26.301550031437753 27.815083658292757 23.118910137271786 44-45 22.782067374328246 26.53773250207596 27.877738674169635 22.802461449426158 46-47 23.06667128419133 26.66936022271861 27.38667343903617 22.8772950540539 48-49 22.90176793475438 26.616720441589276 27.465306036066718 23.01620558758963 50-51 23.041024782639646 26.796496068370146 27.331936532123542 22.830542616866666 52-53 23.2260528437218 26.675247566660705 27.22910040999771 22.869599179619783 54-55 22.86248049576823 26.7738124784756 27.371858880750498 22.991848145005665 56-57 22.85151387469962 26.82650997866319 27.367414513264805 22.95456163337239 58-59 22.98245917819605 26.560078805754205 27.261827116028776 23.19563490002097 60-61 22.73293968935603 26.829973122158542 27.402565419742615 23.03452176874282 62-63 22.77942277093807 26.79584191904325 27.487931906903007 22.936803403115675 64-65 22.51074055475711 26.692043812613154 27.424248545960722 23.372967086669014 66-67 22.507010467361287 26.769682458295534 27.31826415537216 23.405042918971024 68-69 22.802284540104992 26.56577287931129 27.440878337753688 23.191064242830027 70-71 22.67281208839033 26.58561230972402 27.18743061677526 23.554144985110383 72-73 22.754911410865418 26.608466616258692 27.12724551186223 23.509376461013662 74-75 22.515415798475832 26.979484722727264 27.02685282986923 23.478246648927676 76-77 22.723781598779436 26.604772596530314 27.19187161742269 23.47957418726756 78-79 22.72955350460502 26.69454497180424 27.181405228192297 23.394496295398444 80-81 22.711398017284402 26.834115822205906 27.01627969349986 23.43820646700983 82-83 22.696080553415122 26.775679671845072 27.081535609600298 23.446704165139504 84-85 22.686560271986593 26.678813964518216 27.018739483752668 23.615886279742522 86-87 22.70898849922318 26.783236090601687 26.908200616645583 23.599574793529552 88-89 22.638404583048906 26.8904153234154 26.76156709612228 23.709612997413416 90-91 22.575109525706072 26.743127827129282 26.923756255519578 23.75800639164507 92-93 22.54965659762192 26.553718513752738 27.015705678082373 23.880919210542974 94-95 22.438268974326366 26.51442813196657 27.03176415064754 24.015538743059523 96-97 22.48855719670078 26.328028951879624 27.001052410828862 24.182361440590736 98-99 22.325941311985986 26.445449868458166 26.806232739583336 24.422376079972512 100-101 22.488076204548648 26.334012494252146 26.923343318730414 24.254567982468796 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 284.0 1 210.0 2 176.5 3 248.0 4 240.5 5 229.5 6 393.5 7 869.0 8 1428.5 9 1627.0 10 1450.5 11 1266.0 12 1309.0 13 1440.0 14 1581.5 15 1724.5 16 1919.5 17 2098.0 18 2314.5 19 2634.5 20 3039.5 21 3536.5 22 4118.0 23 5089.5 24 6447.0 25 8056.5 26 10576.0 27 14045.5 28 17322.5 29 20869.5 30 25193.5 31 30603.5 32 37733.5 33 41920.5 34 44233.5 35 48523.0 36 54337.5 37 59968.5 38 63860.0 39 68075.0 40 72680.5 41 77733.5 42 81925.0 43 86789.0 44 91860.5 45 95901.0 46 98545.5 47 101996.0 48 105203.5 49 104683.0 50 103599.0 51 100960.5 52 97513.0 53 96053.5 54 95748.5 55 90115.0 56 81876.5 57 74986.5 58 68339.0 59 60460.5 60 49592.5 61 40291.5 62 33064.0 63 25315.5 64 18770.0 65 14184.5 66 10302.5 67 7796.0 68 5902.0 69 4337.0 70 3218.5 71 2234.5 72 1646.5 73 1305.5 74 1001.0 75 785.0 76 543.5 77 283.0 78 164.0 79 109.0 80 63.0 81 29.5 82 12.0 83 7.0 84 7.0 85 4.5 86 1.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.77190582558455E-4 2 0.04875336454010416 3 9.619843042640916E-4 4 0.0 5 0.08900278783051375 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.0783497736450936E-4 26-27 0.008484701563609288 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009600603356555635 36-37 1.1543811651169099E-4 38-39 0.0038479372170563664 40-41 0.005079277126514404 42-43 3.46314349535073E-4 44-45 3.847937217056367E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.619843042640916E-5 68-69 8.657858738376825E-4 70-71 1.5391748868225468E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001385257398140292 82-83 8.657858738376825E-4 84-85 0.005290913673452504 86-87 0.0022125638998074107 88-89 3.847937217056367E-5 90-91 0.00346314349535073 92-93 8.273065016671189E-4 94-95 1.9239686085281835E-5 96-97 0.0 98-99 7.695874434112734E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2598795.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.105172773942755 #Duplication Level Percentage of deduplicated Percentage of total 1 75.47882371455962 42.34752445279336 2 13.877487260986854 15.57197640891714 3 4.60460090467133 7.750257879349142 4 2.0518340800365515 4.604740222556584 5 1.0965298575482612 3.0760498554766014 6 0.6579852666602535 2.2149826241209385 7 0.449807040260097 1.7665551196109994 8 0.30434197304425825 1.3660127184008588 9 0.21722741881773297 1.096882367760588 >10 1.1537908550640672 11.60570662231061 >50 0.07060960458764981 2.7259949536538315 >100 0.033116239652072874 3.4916191383259103 >500 0.002678313934627608 1.0537203055861806 >1k 0.001098795460343769 1.1070459199859033 >5k 6.867471627148556E-5 0.22093141115130063 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5734 0.22064072002601207 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4759 0.1831233321597125 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3474 0.13367733892053818 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.847937217056366E-5 3.847937217056366E-5 10-11 0.0 0.0 0.0 3.847937217056366E-5 3.847937217056366E-5 12-13 0.0 0.0 0.0 3.847937217056366E-5 7.695874434112733E-5 14-15 0.0 0.0 0.0 7.695874434112733E-5 1.5391748868225465E-4 16-17 0.0 0.0 0.0 7.695874434112733E-5 1.5391748868225465E-4 18-19 0.0 0.0 0.0 7.695874434112733E-5 1.9239686085281832E-4 20-21 0.0 0.0 0.0 7.695874434112733E-5 1.9239686085281832E-4 22-23 0.0 0.0 0.0 1.9239686085281834E-4 2.1163654693810013E-4 24-25 0.0 0.0 0.0 4.6175246604676396E-4 2.3087623302338198E-4 26-27 0.0 0.0 0.0 0.0010004636764346553 3.078349773645093E-4 28-29 0.0 0.0 0.0 0.002905192598877557 3.078349773645093E-4 30-31 0.0 0.0 0.0 0.01029323205562578 3.078349773645093E-4 32-33 0.0 0.0 0.0 0.027570470160208867 3.078349773645093E-4 34-35 0.0 0.0 0.0 0.05364024480576575 3.078349773645093E-4 36-37 0.0 0.0 0.0 0.10170098064679978 3.078349773645093E-4 38-39 0.0 0.0 0.0 0.17138712364769054 3.078349773645093E-4 40-41 0.0 0.0 0.0 0.24984656350346987 3.078349773645093E-4 42-43 0.0 0.0 0.0 0.3264205141228915 3.2707466344979115E-4 44-45 0.0 0.0 0.0 0.424408235355232 3.46314349535073E-4 46-47 0.0 0.0 0.0 0.5391922025400233 3.655540356203548E-4 48-49 0.0 0.0 0.0 0.6634998143370292 4.232730938762003E-4 50-51 0.0 0.0 0.0 0.7989664440634987 5.002318382173276E-4 52-53 0.0 0.0 0.0 0.9303927397120588 5.579508964731732E-4 54-55 0.0 0.0 0.0 1.0758640062028748 5.77190582558455E-4 56-57 0.0 0.0 0.0 1.25371566437522 5.77190582558455E-4 58-59 0.0 0.0 0.0 1.4473246254514112 5.77190582558455E-4 60-61 0.0 0.0 0.0 1.656767848175789 5.77190582558455E-4 62-63 0.0 0.0 0.0 1.8604199253884974 6.156699547290186E-4 64-65 0.0 0.0 0.0 2.075827450799313 6.349096408143004E-4 66-67 0.0 0.0 0.0 2.3262319651992556 6.541493268995823E-4 68-69 0.0 0.0 0.0 2.606804307380921 6.541493268995823E-4 70-71 0.0 0.0 0.0 2.905827508518371 6.92628699070146E-4 72-73 0.0 0.0 0.0 3.2080060181738075 7.311080712407097E-4 74-75 0.0 0.0 0.0 3.511762182088237 7.311080712407097E-4 76-77 0.0 0.0 0.0 3.845647694412218 7.695874434112733E-4 78-79 0.0 0.0 0.0 4.219782630026609 7.695874434112733E-4 80-81 0.0 0.0 0.0 4.624797261807876 8.08066815581837E-4 82-83 0.0 0.0 0.0 5.038931504793568 9.235049320935279E-4 84-85 0.0 0.0 0.0 5.453219665267941 9.427446181788097E-4 86-87 0.0 0.0 0.0 5.886035643442441 9.812239903493735E-4 88-89 0.0 0.0 0.0 6.362718105891384 0.0010004636764346553 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4765 0.0 41.624554 1 GTATCAA 7745 0.0 37.82994 1 ATCAACG 9785 0.0 29.781748 3 TCAACGC 9940 0.0 29.269522 4 TATCAAC 9970 0.0 29.148922 2 CAACGCA 10075 0.0 28.877321 5 TAGCGCG 170 3.614332E-9 27.96415 4 AACGCAG 10555 0.0 27.583637 6 ACGCAGA 12045 0.0 24.171463 7 CGCAGAG 12065 0.0 23.895197 8 CGCGCGT 205 2.6755515E-8 23.168365 7 GTACATG 8990 0.0 21.586235 1 TACATGG 8890 0.0 21.24052 2 GCAGAGT 13425 0.0 20.979235 9 AGAGTAC 11650 0.0 20.159958 10-11 ACATGGG 8915 0.0 20.156773 3 AGTACTT 7580 0.0 19.549456 12-13 GAGTACT 7135 0.0 19.437399 12-13 GTACTTT 7455 0.0 18.44379 14-15 GGACCGA 1060 0.0 18.370766 6 >>END_MODULE