FastQCFastQC Report
Thu 26 May 2016
SRR938437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607326
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58290.22356237770037196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45730.17539041914973424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31220.11973953391328895No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45400.045.1486051
GTATCAA75950.039.4151881
ATCAACG94050.031.381253
TCAACGC95450.030.9707624
TATCAAC97050.030.5581112
CAACGCA98200.030.0550565
AACGCAG102250.028.8607356
GTACATG91200.026.4999391
TACATGG90050.025.8612672
ACGCAGA118200.024.877727
CGCAGAG119650.024.6159368
ACATGGG94450.024.0526733
GGACCGA9750.023.3946046
ACCGAGT9950.022.9168828
AGGACCG11450.022.4143285
GCAGAGT128800.021.7607339
GAGTACT68750.021.28030812-13
GTACTTT73000.019.94703514-15
AGAGTAC111450.019.81877910-11
GTGTAGC16750.019.6365871
Produced by FastQC (version 0.11.5)