##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938437_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2607326 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.781397109529074 31.0 31.0 34.0 27.0 34.0 2 30.933016047858995 31.0 31.0 34.0 27.0 34.0 3 30.98669786593621 31.0 31.0 34.0 27.0 34.0 4 34.64583101614451 37.0 35.0 37.0 32.0 37.0 5 34.639014837423474 37.0 35.0 37.0 31.0 37.0 6 34.62757169605949 37.0 35.0 37.0 30.0 37.0 7 34.520944446532575 37.0 35.0 37.0 30.0 37.0 8 34.47779372429838 37.0 35.0 37.0 30.0 37.0 9 35.9112953270899 39.0 35.0 39.0 30.0 39.0 10-11 35.75248089421883 38.0 35.0 39.0 30.0 39.0 12-13 35.56173201970141 38.0 35.0 39.0 30.0 39.0 14-15 36.48581957146901 39.0 36.0 41.0 29.5 41.0 16-17 36.28461860925715 39.0 35.5 40.0 29.0 41.0 18-19 36.122302312790964 38.5 35.0 40.0 27.5 41.0 20-21 36.083752281072634 39.0 35.0 40.0 27.0 41.0 22-23 36.17666222022102 39.0 35.0 40.0 27.0 41.0 24-25 36.07763298490484 39.0 35.0 41.0 27.0 41.0 26-27 35.86199424237705 39.0 35.0 40.0 27.0 41.0 28-29 35.65606774910387 38.0 34.0 40.0 27.0 41.0 30-31 35.382019356229335 38.0 34.0 40.0 26.5 41.0 32-33 35.075080369696764 38.0 34.0 40.0 25.0 41.0 34-35 34.78662219453954 38.0 33.5 40.0 25.0 41.0 36-37 34.47555503224376 38.0 33.0 40.0 24.0 41.0 38-39 34.13458980580104 38.0 33.0 40.0 24.0 41.0 40-41 34.02098241646806 38.0 33.0 40.0 23.0 41.0 42-43 33.77549623637397 38.0 33.0 40.0 22.0 41.0 44-45 33.455997638960376 38.0 32.0 40.0 19.5 41.0 46-47 33.05454668882986 37.0 31.0 40.0 17.0 41.0 48-49 32.651044595113916 37.0 31.0 40.0 14.5 41.0 50-51 31.878049772065324 36.0 30.5 39.0 13.0 40.5 52-53 31.90361159287331 36.5 30.5 39.0 10.0 40.0 54-55 31.943377237829104 36.5 30.5 39.0 8.5 41.0 56-57 31.577040807325204 36.0 30.0 39.0 7.5 41.0 58-59 31.097566625730728 36.0 29.0 39.0 2.0 40.5 60-61 30.561750045832397 35.0 28.0 39.0 2.0 40.0 62-63 29.935409304398455 35.0 28.0 38.5 2.0 40.0 64-65 29.39510671086009 34.0 26.5 38.0 2.0 40.0 66-67 28.798734795725583 34.0 26.0 38.0 2.0 40.0 68-69 28.1358031178303 34.0 25.5 37.0 2.0 39.5 70-71 27.461634256705914 33.0 23.5 36.0 2.0 39.0 72-73 26.77899426462207 32.5 22.0 36.0 2.0 39.0 74-75 26.046404438877225 32.0 20.5 35.0 2.0 38.0 76-77 25.26399134592299 31.5 17.5 35.0 2.0 37.0 78-79 24.435642109962465 31.0 10.0 35.0 2.0 36.5 80-81 23.647760195694744 30.5 4.5 34.0 2.0 36.0 82-83 22.890807286852507 30.0 2.0 34.0 2.0 36.0 84-85 22.137475137362955 29.0 2.0 34.0 2.0 35.0 86-87 21.39153063330017 29.0 2.0 34.0 2.0 35.0 88-89 20.593262599306723 28.0 2.0 34.0 2.0 35.0 90-91 19.751538165921716 26.5 2.0 34.0 2.0 35.0 92-93 18.873951512008855 25.0 2.0 33.0 2.0 35.0 94-95 17.971583338638894 23.5 2.0 33.0 2.0 35.0 96-97 16.905793521792056 18.5 2.0 32.0 2.0 34.5 98-99 15.677784634525947 2.0 2.0 32.0 2.0 34.0 100-101 13.092446053926514 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7555058119231965 1101 2 0.7643258963946842 1101 3 0.6623865905072677 1101 4 0.6118406925534643 1101 5 0.5760959270614165 1101 6 0.5237708553553588 1101 7 0.5582086210666262 1101 8 0.5098016722097256 1101 9 0.7710336977331025 1101 10-11 0.902619323315335 1101 12-13 0.9303316507952175 1101 14-15 0.9715955726732375 1101 16-17 1.234230869440161 1101 18-19 1.2033889933411999 1101 20-21 1.2524490680705611 1101 22-23 1.0481308935103542 1101 24-25 1.1965612946589417 1101 26-27 1.2534980438560055 1101 28-29 1.4098503025753004 1101 30-31 1.3638749345609185 1101 32-33 1.3730629086668884 1101 34-35 1.436198949262753 1101 36-37 1.5433241607316646 1101 38-39 1.6721994233329553 1101 40-41 1.7022469668179596 1101 42-43 2.018324792492457 1101 44-45 2.0305666522786012 1101 46-47 2.1580826110802107 1101 48-49 2.1870057096194415 1101 50-51 2.1768173040940404 1101 52-53 2.357028476838668 1101 54-55 2.5800122520659237 1101 56-57 2.6957277923832024 1101 58-59 2.8081649990225124 1101 60-61 3.0157226979587826 1101 62-63 2.884321075804497 1101 64-65 2.937152000285476 1101 66-67 3.17238502552728 1101 68-69 3.3633059551064157 1101 70-71 3.506769977474409 1101 72-73 3.6455983000981753 1101 74-75 3.8427685905852513 1101 76-77 4.136267968056124 1101 78-79 4.338277454831616 1101 80-81 4.891091383616629 1101 82-83 5.075904828479288 1101 84-85 5.232013591748448 1101 86-87 5.439582667377188 1101 88-89 5.753965614126095 1101 90-91 5.931851716237649 1101 92-93 5.877030740050955 1101 94-95 6.076090646413597 1101 96-97 6.2815029805711795 1101 98-99 6.391388470781889 1101 100-101 6.152803944248751 1102 1 0.7737625104744055 1102 2 0.7239418035059764 1102 3 0.6656060105122208 1102 4 0.7270355958210288 1102 5 0.5753927090398534 1102 6 0.5797745792144298 1102 7 0.624663722993283 1102 8 0.7627653765704778 1102 9 0.8167948113747343 1102 10-11 0.7580176204089639 1102 12-13 0.7788785666248117 1102 14-15 1.1006340818510338 1102 16-17 1.1908111521657645 1102 18-19 1.2140061870218304 1102 20-21 1.299304964313933 1102 22-23 1.1670271808388435 1102 24-25 1.0991241447696183 1102 26-27 1.0495718098282438 1102 28-29 1.121934464986694 1102 30-31 1.3362985994455698 1102 32-33 1.2910411168993434 1102 34-35 1.395167076705036 1102 36-37 1.463597316981911 1102 38-39 1.4273736695711463 1102 40-41 1.720850779378388 1102 42-43 1.8259706902541453 1102 44-45 2.1410048450082897 1102 46-47 2.1102537967217785 1102 48-49 1.9986401720971685 1102 50-51 2.316227779645228 1102 52-53 2.4030632861307346 1102 54-55 2.5671850159524787 1102 56-57 2.661929376221977 1102 58-59 2.8043392532518148 1102 60-61 2.7628019458351467 1102 62-63 3.0888346567025415 1102 64-65 3.1241645423387077 1102 66-67 3.1115966222317475 1102 68-69 3.3199536628554007 1102 70-71 3.4399323009706784 1102 72-73 3.76037067321926 1102 74-75 3.8109644567820418 1102 76-77 3.9149365885501375 1102 78-79 4.30751915805763 1102 80-81 4.876271363479024 1102 82-83 4.993681760530848 1102 84-85 4.985366862910915 1102 86-87 5.23387591328774 1102 88-89 5.523274642486559 1102 90-91 5.793852171731139 1102 92-93 5.651623393020852 1102 94-95 5.888461789702262 1102 96-97 5.997987250063506 1102 98-99 6.042423020362756 1102 100-101 5.924080284957887 1103 1 0.6714095060961007 1103 2 0.6872643669453851 1103 3 0.6442772716514504 1103 4 0.7180455483797772 1103 5 0.651928985803103 1103 6 0.5839613435008459 1103 7 0.7310058730580664 1103 8 0.6119059595005751 1103 9 0.8884516994381855 1103 10-11 0.7630532173386513 1103 12-13 0.9047544199342568 1103 14-15 0.9864143683404407 1103 16-17 1.0886770311720184 1103 18-19 1.2334554706703926 1103 20-21 1.220759884889162 1103 22-23 1.221301786273159 1103 24-25 1.2228648857945998 1103 26-27 1.2231602438319413 1103 28-29 1.2591962514332593 1103 30-31 1.2209990186016526 1103 32-33 1.3816471664587553 1103 34-35 1.3631395930101675 1103 36-37 1.537907186783876 1103 38-39 1.5045435729618646 1103 40-41 1.5422571267087477 1103 42-43 1.7424667589870495 1103 44-45 1.6402660136568983 1103 46-47 1.9212819698433492 1103 48-49 1.945450710220932 1103 50-51 2.0167019363766023 1103 52-53 2.1471868508377163 1103 54-55 2.3268090097924095 1103 56-57 2.4948470069987962 1103 58-59 2.7820232208193367 1103 60-61 3.0159005287371023 1103 62-63 3.052304474778314 1103 64-65 3.253241305333251 1103 66-67 3.4666609665920696 1103 68-69 3.6608224044534126 1103 70-71 3.7184755576996267 1103 72-73 3.707585402136427 1103 74-75 4.00112199526011 1103 76-77 4.1561297700049415 1103 78-79 4.245384736478957 1103 80-81 4.744825895340387 1103 82-83 5.045897519111268 1103 84-85 5.37783548376332 1103 86-87 5.444005881093172 1103 88-89 5.682186377961592 1103 90-91 5.8190730057200035 1103 92-93 6.002867590772475 1103 94-95 6.031823531508763 1103 96-97 6.1948078609799815 1103 98-99 6.416102045546657 1103 100-101 6.213783556473052 1104 1 0.5864806425709155 1104 2 0.5840106821173201 1104 3 0.5219714542096732 1104 4 0.5281266136718514 1104 5 0.46351664655648506 1104 6 0.49700368856471755 1104 7 0.5374625653399931 1104 8 0.5824296483199092 1104 9 0.6348041931166222 1104 10-11 0.7202393954626842 1104 12-13 0.7447312033429156 1104 14-15 0.9293744081667512 1104 16-17 1.1031981963012996 1104 18-19 1.2949858379225034 1104 20-21 1.1643890943036155 1104 22-23 1.1319264689612396 1104 24-25 1.142655449130828 1104 26-27 1.3001386508906307 1104 28-29 1.043184792116115 1104 30-31 1.2515170999063017 1104 32-33 1.346998940845431 1104 34-35 1.4607677962798036 1104 36-37 1.6639060831706942 1104 38-39 1.6600110694255577 1104 40-41 1.713932129592699 1104 42-43 1.8304850762514633 1104 44-45 1.9609297206991059 1104 46-47 2.0807692647202742 1104 48-49 2.246272744730895 1104 50-51 2.17344430963227 1104 52-53 2.2954954356404293 1104 54-55 2.5010619889002506 1104 56-57 2.645714752932122 1104 58-59 2.791797276224546 1104 60-61 2.9901294029344534 1104 62-63 3.066516733089184 1104 64-65 3.180465989625244 1104 66-67 3.3143239469398615 1104 68-69 3.692743752428381 1104 70-71 3.825892236138575 1104 72-73 3.950565367454967 1104 74-75 3.744906305816489 1104 76-77 3.9981462174809117 1104 78-79 4.343184511808236 1104 80-81 4.614124690742596 1104 82-83 4.718880402311488 1104 84-85 4.813651957025222 1104 86-87 4.9541654145775595 1104 88-89 5.388143251917121 1104 90-91 5.801013831943184 1104 92-93 5.718822373476655 1104 94-95 6.0916866810073245 1104 96-97 6.132082272638183 1104 98-99 6.186287941752729 1104 100-101 5.98536454557942 1105 1 0.6589967472497094 1105 2 0.5615621820504835 1105 3 0.5577128288799713 1105 4 0.5043271991466298 1105 5 0.4868696821978986 1105 6 0.5352783222896491 1105 7 0.5333631524047817 1105 8 0.5095613872129974 1105 9 0.6665702634925523 1105 10-11 0.6486546208692943 1105 12-13 0.7543765647419818 1105 14-15 0.7872482990653893 1105 16-17 0.9592568327021453 1105 18-19 0.9781065180437523 1105 20-21 0.9447850376735687 1105 22-23 0.9233515188255339 1105 24-25 0.9938603040606395 1105 26-27 1.0356993540021477 1105 28-29 1.0393314617350882 1105 30-31 1.0583297809532795 1105 32-33 1.0658263983290226 1105 34-35 1.214117257356797 1105 36-37 1.331720767033751 1105 38-39 1.4121542622359087 1105 40-41 1.558490694443364 1105 42-43 1.6127330797659596 1105 44-45 1.5205963852065096 1105 46-47 1.5659848044765567 1105 48-49 1.568054650368282 1105 50-51 1.628069719426339 1105 52-53 1.8603157004173063 1105 54-55 1.9300928255544356 1105 56-57 2.1421155287362623 1105 58-59 2.155305669973121 1105 60-61 2.6498329319776452 1105 62-63 2.8295473306538845 1105 64-65 2.984686301748905 1105 66-67 3.0195693846591105 1105 68-69 3.1265999565943083 1105 70-71 3.397450289832424 1105 72-73 3.615266219991522 1105 74-75 3.726318055592266 1105 76-77 3.7928067781398553 1105 78-79 3.9074473918707078 1105 80-81 4.155356510961436 1105 82-83 4.512506724296706 1105 84-85 4.597754817913426 1105 86-87 4.916875111069366 1105 88-89 5.108475545073805 1105 90-91 5.345717970487886 1105 92-93 5.47107267071906 1105 94-95 5.612169906760208 1105 96-97 5.8659959230575645 1105 98-99 6.107587237027653 1105 100-101 5.420545090526771 1106 1 0.41286973562674234 1106 2 0.4339152495898233 1106 3 0.33783004119674587 1106 4 0.3809708910718044 1106 5 0.3624032964690471 1106 6 0.21664947101148613 1106 7 0.3591580891252093 1106 8 0.3955090495939899 1106 9 0.4557063568419153 1106 10-11 0.31475177523121545 1106 12-13 0.1968544888796302 1106 14-15 0.7643228816579111 1106 16-17 0.4750970066581104 1106 18-19 0.6525841016737886 1106 20-21 0.7759146888018194 1106 22-23 0.6454370365113604 1106 24-25 0.7531754152855044 1106 26-27 0.7895997658106779 1106 28-29 0.649471961115772 1106 30-31 0.9111233716402793 1106 32-33 0.9932794242761602 1106 34-35 1.037535782341827 1106 36-37 1.1467205842108754 1106 38-39 1.0060718022626958 1106 40-41 1.1467015897726967 1106 42-43 0.7323900811965558 1106 44-45 1.107837747968233 1106 46-47 1.6175108613070464 1106 48-49 1.512181693985248 1106 50-51 0.8865552718485183 1106 52-53 1.5422950208646746 1106 54-55 1.3420476134570514 1106 56-57 1.377755077437996 1106 58-59 0.9028027105278049 1106 60-61 2.141147702694905 1106 62-63 2.3685423075953516 1106 64-65 2.5082218905071265 1106 66-67 2.548119747791052 1106 68-69 1.8706724961411858 1106 70-71 2.2503170096700735 1106 72-73 2.7983476195920574 1106 74-75 3.134291304034843 1106 76-77 3.2841583990931866 1106 78-79 3.590598778353698 1106 80-81 3.947862544116365 1106 82-83 4.161156291692077 1106 84-85 4.388283221732593 1106 86-87 4.681043312686434 1106 88-89 4.609425367050029 1106 90-91 4.880823105068377 1106 92-93 5.221831099996315 1106 94-95 5.080080207001078 1106 96-97 5.349481304083287 1106 98-99 5.265899279113478 1106 100-101 4.620486758602697 1107 1 0.5365331616461191 1107 2 0.40869048345579984 1107 3 0.44583677287490175 1107 4 0.4479642145442142 1107 5 0.45293722564910155 1107 6 0.3854723869543051 1107 7 0.49904171039965206 1107 8 0.4375455008094562 1107 9 0.5000017752637476 1107 10-11 0.486086179037045 1107 12-13 0.5821190433898948 1107 14-15 0.6999337679385391 1107 16-17 0.838387369427501 1107 18-19 1.0412756434519963 1107 20-21 0.8679298932615112 1107 22-23 0.7375476514512016 1107 24-25 0.7987443292274534 1107 26-27 0.7371586087076736 1107 28-29 0.944903205455951 1107 30-31 0.9886652750407521 1107 32-33 1.0662447268487156 1107 34-35 1.1894425718177786 1107 36-37 1.1293525677892333 1107 38-39 1.4233715284952808 1107 40-41 1.373782117320964 1107 42-43 1.4626249453993125 1107 44-45 1.5561419507723073 1107 46-47 1.4503308118038944 1107 48-49 1.66385595488493 1107 50-51 1.819848248754763 1107 52-53 1.9243644273011888 1107 54-55 2.1073332823617186 1107 56-57 2.1821378791750163 1107 58-59 2.286453490522266 1107 60-61 2.3229931840435363 1107 62-63 2.5168489370243528 1107 64-65 2.5508247380411753 1107 66-67 2.730515838608593 1107 68-69 2.882020552469765 1107 70-71 2.850457960730566 1107 72-73 3.222646366350581 1107 74-75 3.4201160090620384 1107 76-77 3.39958970685651 1107 78-79 3.479377182274238 1107 80-81 3.978724318596875 1107 82-83 4.091895659490099 1107 84-85 4.242317182348472 1107 86-87 4.497301616982995 1107 88-89 4.874305886105098 1107 90-91 4.948177595813558 1107 92-93 5.126082753686049 1107 94-95 5.092729885638725 1107 96-97 5.097748611158334 1107 98-99 5.194615103686145 1107 100-101 5.009704092094863 1108 1 0.32229844058758417 1108 2 0.35744305631524753 1108 3 0.21112167348237776 1108 4 0.2644679384118902 1108 5 0.26068816212822554 1108 6 0.3500770713958943 1108 7 0.2894988202715254 1108 8 0.27804192115301163 1108 9 0.35032601216585135 1108 10-11 0.4494122302991741 1108 12-13 0.3846825469838535 1108 14-15 0.34144352047128024 1108 16-17 0.651745063732271 1108 18-19 0.6936832757618276 1108 20-21 0.45502612664692776 1108 22-23 0.5723193229626489 1108 24-25 0.633169774603779 1108 26-27 0.6640178907264414 1108 28-29 0.7431926139555074 1108 30-31 0.6854887743997509 1108 32-33 0.8762819873756342 1108 34-35 0.9789054982092651 1108 36-37 1.1362188895407783 1108 38-39 1.1948330795168616 1108 40-41 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58-59 0.8349677510337266 2311 60-61 0.785074848381857 2311 62-63 1.116424361455877 2311 64-65 1.1755401773384726 2311 66-67 1.1575353160226136 2311 68-69 0.9400673346159572 2311 70-71 1.2326650203502894 2311 72-73 1.3877854671649494 2311 74-75 1.4771336321826674 2311 76-77 1.6163603370491195 2311 78-79 1.8882420011427747 2311 80-81 2.0325140176303904 2311 82-83 2.1358804548080634 2311 84-85 2.580893870737608 2311 86-87 2.5655933046676047 2311 88-89 2.5025015957730403 2311 90-91 2.868494968080764 2311 92-93 3.1033537130943145 2311 94-95 3.1872965571768432 2311 96-97 3.2609723945140097 2311 98-99 3.507899413483047 2311 100-101 3.2489307915102383 2312 1 -0.39764108935615994 2312 2 -0.3630935905381705 2312 3 -0.3017711109017327 2312 4 -0.3833495207656554 2312 5 -0.3430878075739301 2312 6 -0.3337512484917724 2312 7 -0.34961084356199024 2312 8 -0.4350079750696807 2312 9 -0.42903182096237913 2312 10-11 -0.5271569634474531 2312 12-13 -0.4937522636680356 2312 14-15 -0.40543751078294576 2312 16-17 -0.37987359802876597 2312 18-19 -0.32400731809564576 2312 20-21 -0.3849607130342676 2312 22-23 -0.30176790972794976 2312 24-25 -0.4360689760238827 2312 26-27 -0.1504434458579098 2312 28-29 -0.30254179196678166 2312 30-31 -0.17056028034388504 2312 32-33 -0.286959970600968 2312 34-35 -0.28933979389351094 2312 36-37 -0.24025700229488223 2312 38-39 -0.022054796175588365 2312 40-41 -0.14480543245576172 2312 42-43 -0.02900462365929002 2312 44-45 0.09583224694788584 2312 46-47 -0.007239702566153028 2312 48-49 0.41604277647290644 2312 50-51 0.4862217199844103 2312 52-53 0.5888975718791194 2312 54-55 0.4892433744917888 2312 56-57 0.2740923342228143 2312 58-59 0.43054468683289926 2312 60-61 0.5938049518019071 2312 62-63 0.5801161172938656 2312 64-65 0.6055487700046847 2312 66-67 0.7925778526241452 2312 68-69 1.0118872401092567 2312 70-71 1.20887598532968 2312 72-73 1.3216205485743409 2312 74-75 1.2685736776937482 2312 76-77 1.359774499015451 2312 78-79 1.3736142052437224 2312 80-81 1.6296197309075389 2312 82-83 1.8847734199318857 2312 84-85 1.9618330494069909 2312 86-87 2.3098641451025763 2312 88-89 2.133289020331553 2312 90-91 2.2082051303541412 2312 92-93 2.3163858688056465 2312 94-95 2.401391622920965 2312 96-97 2.4140027801815513 2312 98-99 2.343413233712246 2312 100-101 2.3513151364450593 2313 1 -0.6772980153345287 2313 2 -0.6523548341783716 2313 3 -0.6627181855953417 2313 4 -0.720758973663159 2313 5 -0.633599261207415 2313 6 -0.5866519056707418 2313 7 -0.5979171477602563 2313 8 -0.5321486165691525 2313 9 -0.7250518483214989 2313 10-11 -0.5693899869907 2313 12-13 -0.6047120879536578 2313 14-15 -0.6319206383228604 2313 16-17 -0.4599102243567259 2313 18-19 -0.4317956188700194 2313 20-21 -0.4528546654277932 2313 22-23 -0.44577298906541074 2313 24-25 -0.47625481507703427 2313 26-27 -0.27196524466035044 2313 28-29 -0.3093766982497854 2313 30-31 -0.2005518206145993 2313 32-33 -0.10409144739370646 2313 34-35 -0.2201061343706172 2313 36-37 -0.05512611398330591 2313 38-39 -0.05683480821519282 2313 40-41 -0.11496619981120659 2313 42-43 0.03003592454315651 2313 44-45 0.07373932892127044 2313 46-47 -0.07536358510191121 2313 48-49 -0.040225997926306434 2313 50-51 0.2536730365422777 2313 52-53 0.41159174566555023 2313 54-55 0.39771355344606363 2313 56-57 0.5239910874627043 2313 58-59 0.593516971719783 2313 60-61 0.5690676808945767 2313 62-63 0.711338543176236 2313 64-65 0.7003667983277779 2313 66-67 0.8508401757298039 2313 68-69 1.0653697161700215 2313 70-71 1.1054816064498034 2313 72-73 1.2210476419073544 2313 74-75 1.098997729124104 2313 76-77 1.2398099628806065 2313 78-79 1.435270357246857 2313 80-81 1.554116084524221 2313 82-83 1.6511197718486237 2313 84-85 1.9682186575675296 2313 86-87 1.8739944679322456 2313 88-89 1.856753431970379 2313 90-91 2.0448341082268477 2313 92-93 2.0004966022621744 2313 94-95 2.111508836670126 2313 96-97 2.5146391848166054 2313 98-99 2.58523012057187 2313 100-101 2.2510344704263705 2314 1 -0.562083218843 2314 2 -0.47924841350778635 2314 3 -0.6545370695393444 2314 4 -0.5714789226780255 2314 5 -0.4082357760708746 2314 6 -0.4389327343952516 2314 7 -0.38414954060505835 2314 8 -0.42568203775822866 2314 9 -0.5824392921514345 2314 10-11 -0.42868802319878796 2314 12-13 -0.48536555105425094 2314 14-15 -0.6115090305627504 2314 16-17 -0.5924844066501933 2314 18-19 -0.4486439380888285 2314 20-21 -0.44252936513397856 2314 22-23 -0.6338529179886692 2314 24-25 -0.5662038839501733 2314 26-27 -0.4254357715787762 2314 28-29 -0.3890423243299779 2314 30-31 -0.42826202628345555 2314 32-33 -0.5095704592326413 2314 34-35 -0.523339423776072 2314 36-37 -0.421108523031009 2314 38-39 -0.23840589575408444 2314 40-41 -0.2270234554714179 2314 42-43 -0.06445468317373582 2314 44-45 -0.040951573688481346 2314 46-47 -0.07808634802101722 2314 48-49 -0.13873290036323027 2314 50-51 -0.12888966869449447 2314 52-53 0.0977361027343342 2314 54-55 0.18559864245400703 2314 56-57 0.11380648238147728 2314 58-59 0.6153098770084107 2314 60-61 0.7325651046329078 2314 62-63 0.694127008869291 2314 64-65 0.8035799691279841 2314 66-67 0.9225886580578369 2314 68-69 1.128519857675382 2314 70-71 1.2152326488366008 2314 72-73 1.1383034641506988 2314 74-75 1.296183014727287 2314 76-77 1.2342321133299663 2314 78-79 1.4472618561234576 2314 80-81 1.8221762459051405 2314 82-83 1.9028359293840573 2314 84-85 2.1153822042751003 2314 86-87 2.1851806825018976 2314 88-89 2.2960634313222634 2314 90-91 2.229674131559026 2314 92-93 2.2772057385393936 2314 94-95 2.40944715221627 2314 96-97 2.7041875018750225 2314 98-99 2.5872676356945785 2314 100-101 2.4022629205646364 2315 1 -0.5729743872661075 2315 2 -0.6589920112288183 2315 3 -0.5525310298869499 2315 4 -0.4009931120885213 2315 5 -0.3129605902132724 2315 6 -0.3298702670843099 2315 7 -0.5247969597891213 2315 8 -0.5958501137342864 2315 9 -0.6804741390888509 2315 10-11 -0.588774762053518 2315 12-13 -0.571162532109966 2315 14-15 -0.6026133188041456 2315 16-17 -0.4308768532385585 2315 18-19 -0.39042978789907323 2315 20-21 -0.533955724074147 2315 22-23 -0.3754014712427818 2315 24-25 -0.5773106930004914 2315 26-27 -0.5147581492331881 2315 28-29 -0.7355566016870654 2315 30-31 -0.6878482657534377 2315 32-33 -0.5524922531568706 2315 34-35 -0.5367348652030373 2315 36-37 -0.33019685986791814 2315 38-39 -0.3067092223154475 2315 40-41 -0.2223671857737699 2315 42-43 -0.23767357488502938 2315 44-45 -0.3060608712108106 2315 46-47 -0.11770604149163688 2315 48-49 -0.13936763466041668 2315 50-51 0.07833674820712133 2315 52-53 0.2771176863265943 2315 54-55 0.4002751031206877 2315 56-57 0.5316696962830179 2315 58-59 0.6676413435386088 2315 60-61 0.7324223445357738 2315 62-63 0.8691511941813275 2315 64-65 0.7642184197197643 2315 66-67 1.0735242358338546 2315 68-69 1.17595565115327 2315 70-71 1.264354187348264 2315 72-73 1.386290246684755 2315 74-75 1.4437828690152195 2315 76-77 1.5518994807112882 2315 78-79 1.8702669445318243 2315 80-81 1.9904879506652549 2315 82-83 2.0203098510155577 2315 84-85 2.1544362468417404 2315 86-87 2.35522529028583 2315 88-89 2.5245539371327403 2315 90-91 2.576015149235804 2315 92-93 2.58114363893829 2315 94-95 2.6231295591992776 2315 96-97 2.724335171302659 2315 98-99 2.6866474040104347 2315 100-101 2.7935346012974467 2316 1 -0.9800356488439697 2316 2 -0.882412756419857 2316 3 -0.7315727901375269 2316 4 -0.734195022029148 2316 5 -0.7376034709460342 2316 6 -0.7236674994912775 2316 7 -0.7037568566974173 2316 8 -0.7989515357846955 2316 9 -0.9066241724079944 2316 10-11 -0.7173123869301747 2316 12-13 -0.9323805132783392 2316 14-15 -1.2103118138192812 2316 16-17 -1.0411439062526 2316 18-19 -0.9469792505720704 2316 20-21 -0.8037551129808662 2316 22-23 -0.9912192924078482 2316 24-25 -1.0524723731015015 2316 26-27 -0.8509218034903654 2316 28-29 -1.0010134718194124 2316 30-31 -0.7694272540602398 2316 32-33 -0.6471631918488825 2316 34-35 -0.7148001309439351 2316 36-37 -0.5787115247548442 2316 38-39 -0.5476656182269721 2316 40-41 -0.7247379073621616 2316 42-43 -0.7063129015756147 2316 44-45 -0.5862727799553724 2316 46-47 -0.376392417926823 2316 48-49 -0.25152653421307747 2316 50-51 -0.1421789959497879 2316 52-53 -0.14211615694820523 2316 54-55 -0.3295241608233006 2316 56-57 0.02457557460654769 2316 58-59 0.15510254452229688 2316 60-61 0.06789736486771858 2316 62-63 0.2532990720208197 2316 64-65 0.3738102914294501 2316 66-67 0.6279882234865184 2316 68-69 0.7185891107159676 2316 70-71 0.45617600120020896 2316 72-73 1.0877778005239271 2316 74-75 1.2863915400518415 2316 76-77 1.3638644840049459 2316 78-79 1.2951279999976855 2316 80-81 1.4634760442669332 2316 82-83 1.7939701421175265 2316 84-85 1.782227091867803 2316 86-87 2.212651353788832 2316 88-89 2.30666215537072 2316 90-91 2.528057257086804 2316 92-93 2.717260444921404 2316 94-95 2.7276857514980577 2316 96-97 2.5611968954466526 2316 98-99 2.5097002997157727 2316 100-101 2.487174049125379 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11762.0 3 4729.0 4 5314.0 5 5718.0 6 6594.0 7 7800.0 8 10082.0 9 12639.0 10 15165.0 11 18205.0 12 21322.0 13 24742.0 14 27827.0 15 31393.0 16 34743.0 17 39121.0 18 39403.0 19 39579.0 20 42485.0 21 46489.0 22 51543.0 23 56541.0 24 62281.0 25 68831.0 26 75789.0 27 84048.0 28 91845.0 29 101411.0 30 112829.0 31 126707.0 32 142854.0 33 162220.0 34 187875.0 35 217368.0 36 246393.0 37 239802.0 38 125070.0 39 8807.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.435583435729626 18.462955368553555 14.15657899697543 23.94488219874139 2 15.639392214066367 19.48367200676477 39.9992480176056 24.877687761563262 3 19.6335232210544 23.27649747129718 28.937599495372133 28.152379812276283 4 13.024705308608201 15.738156803715448 36.28024085632689 34.95689703134946 5 14.170408543490515 36.966583482579885 33.90249770997522 14.960510263954378 6 33.59165917719989 35.02468645932548 16.97601810058936 14.407636262885273 7 29.711482685278114 31.665584784283396 20.03941581420473 18.58351671623376 8 26.725414916387525 35.05002323048361 19.221796063593594 19.00276578953527 9 26.4305822163919 16.574307092164023 19.64655628536864 37.348554406075436 10-11 25.356244222451007 26.15776854584835 26.992248517043805 21.493738714656843 12-13 26.16786878956455 23.26936504891617 27.318012660656056 23.244753500863226 14-15 22.92144974134232 24.865185149947415 25.41349838421671 26.799866724493555 16-17 22.846894311413195 28.093326597055906 26.163475182610274 22.89630390892062 18-19 22.877888469943645 26.86286398571976 27.968754106039544 22.29049343829705 20-21 23.30553256143427 26.05284992283248 28.272454598733667 22.36916291699959 22-23 23.021299549516712 25.96718477487729 28.04164457474946 22.96987110085654 24-25 22.884596493328484 26.11780334949601 28.136986742921344 22.86061341425416 26-27 22.56506743008256 26.4478368923724 27.987983183055974 22.99911249448906 28-29 22.63705628891527 26.727053021936335 27.98176093426006 22.654129754888334 30-31 22.688592330557775 26.3059240153738 27.994241060166726 23.011242593901702 32-33 22.521536944331107 26.31310187052838 27.936959370376357 23.22840181476415 34-35 23.154595118436582 26.430762224673764 27.758547167172804 22.65609548971685 36-37 23.182813140513925 26.023867952632397 27.75581280923305 23.03750609762063 38-39 22.75162202373246 26.4758847981943 27.741319365525808 23.031173812547433 40-41 23.103049394837434 26.224254721625982 27.7883961230775 22.884299760459083 42-43 22.843678835096036 26.20408643947091 27.692899625849904 23.259335099583154 44-45 22.763151308442346 26.487881021300197 27.774584560516452 22.974383109741 46-47 23.102605019774522 26.651910897659665 27.22698311014274 23.018500972423077 48-49 22.97386611225335 26.579157332678676 27.331048424218245 23.115928130849724 50-51 23.05724949725123 26.79281764459277 27.195515285365303 22.954417572790696 52-53 23.175763024524525 26.65678763841683 27.19299372719422 22.974455609864428 54-55 22.843344019948177 26.699078746933203 27.380779550740257 23.076797682378363 56-57 22.88782776409021 26.807140940740254 27.26782823586223 23.037203059307306 58-59 22.94751381109513 26.525990327342996 27.254910835347353 23.27158502621452 60-61 22.76758642618597 26.719999140829877 27.381521782072266 23.130892650911893 62-63 22.8103534310392 26.67965755003523 27.50762979472717 23.002359224198408 64-65 22.60226092609942 26.646996155716035 27.430064045828388 23.320678872356158 66-67 22.61780452111772 26.690346757612943 27.297230591501748 23.39461812976759 68-69 22.854849706143796 26.43240636060361 27.458338007991507 23.254405925261086 70-71 22.752801069276874 26.46364368484717 27.22273590422385 23.560819341652106 72-73 22.76210482869781 26.550233382489708 27.182361703829304 23.50530008498318 74-75 22.629615757050612 26.828020742893866 27.120668231263434 23.421695268792085 76-77 22.83220426456399 26.467637570555212 27.167747789249276 23.532410375631525 78-79 22.895556644857614 26.523124271721027 27.165870018648548 23.415449064772815 80-81 22.85898723282169 26.634099865217014 27.009106481627093 23.497806420334204 82-83 22.86512900681182 26.586743797068944 27.065203152956443 23.482924043162797 84-85 22.92771573942832 26.448946355946685 27.00732026740426 23.616017637220736 86-87 22.967028625869293 26.439999815900944 26.99034093611302 23.60263062211674 88-89 22.93729980627961 26.475190363849087 26.89972572261542 23.687784107255883 90-91 22.906531742577453 26.397533339868023 26.939656867380762 23.75627805017376 92-93 22.955837693714262 26.16899827656112 27.070610361270077 23.80455366845455 94-95 22.83221333619766 26.050554755824002 27.143801110758396 23.973430797219937 96-97 22.97103578541639 25.85002255576799 27.063680150003528 24.115261508812093 98-99 22.879797223094872 25.8900162370354 26.868170078822313 24.362016461047418 100-101 23.336027890296272 25.457765939046702 27.03948559365454 24.166720577002483 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 322.0 1 232.0 2 185.5 3 265.5 4 246.0 5 253.0 6 423.0 7 893.0 8 1484.0 9 1712.5 10 1573.0 11 1424.0 12 1461.0 13 1561.5 14 1688.0 15 1834.5 16 2017.0 17 2220.0 18 2434.5 19 2702.5 20 3086.0 21 3637.0 22 4378.5 23 5466.0 24 6868.5 25 8733.5 26 11265.0 27 14309.5 28 17398.5 29 20920.0 30 25186.5 31 30443.5 32 36772.5 33 40848.0 34 43805.5 35 48262.0 36 54003.0 37 59418.0 38 63180.5 39 67246.5 40 72090.5 41 77145.0 42 81329.0 43 85266.5 44 89405.0 45 93275.0 46 96478.5 47 99763.5 48 102383.5 49 102729.5 50 101662.0 51 99009.5 52 96027.0 53 94370.5 54 93603.0 55 89086.0 56 81983.0 57 75857.5 58 69905.5 59 62266.0 60 52306.0 61 43669.5 62 36052.0 63 28210.5 64 21966.5 65 17369.0 66 13281.5 67 10228.5 68 7854.0 69 5809.5 70 4384.0 71 3327.0 72 2499.5 73 1911.0 74 1479.0 75 1094.5 76 797.5 77 552.0 78 334.0 79 195.0 80 133.5 81 79.5 82 43.0 83 30.5 84 19.5 85 15.0 86 14.0 87 9.5 88 6.0 89 5.0 90 3.5 91 1.0 92 1.5 93 2.5 94 2.5 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30483338101948126 2 0.03382776070196055 3 0.040232790222626554 4 0.043147653956582335 5 0.055957712997914334 6 0.05538241094515991 7 0.029071930399190586 8 0.03210185454369726 9 0.039389013878586725 10-11 0.030395125120525782 12-13 0.031679966371677346 14-15 0.026674838512713787 16-17 0.020902641250077666 18-19 0.024833871943899615 20-21 0.03772063792559887 22-23 0.030375948385433962 24-25 0.050703287582757196 26-27 0.04391472336025491 28-29 0.03614814564807009 30-31 0.036186499118253716 32-33 0.03131560840493287 34-35 0.030855366762729323 36-37 0.029781469597587724 38-39 0.01819872160213184 40-41 0.01781518690029555 42-43 0.018582256303968127 44-45 0.009108949168611827 46-47 0.016722113000062135 48-49 0.014037370087208121 50-51 0.00609820175919697 52-53 0.004583239686943635 54-55 0.006539266666308701 56-57 0.005158541739698066 58-59 0.007018685043604061 60-61 0.00609820175919697 62-63 0.004333942130750048 64-65 0.007996698533286594 66-67 0.002780626588313084 68-69 0.007037861778695875 70-71 0.012675821895689299 72-73 0.013021003127341958 74-75 0.010144492863569802 76-77 0.009070595698428198 78-79 0.005829727467911569 80-81 0.0065967968715841445 82-83 0.011985459432383983 84-85 0.0043147653956582335 86-87 0.0013040179862433773 88-89 0.018256251807407285 90-91 0.027787089148039026 92-93 0.012618291690413857 94-95 0.021036878395720365 96-97 0.017412475463367452 98-99 0.012388170869312085 100-101 0.10326671846942038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2607326.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.1462427832233 #Duplication Level Percentage of deduplicated Percentage of total 1 77.58053310109848 45.88597046049463 2 12.862382001974742 15.2152313731872 3 4.264028266003896 7.566037531667792 4 1.8030730306382925 4.265799809040587 5 0.9858371035844686 2.9154280336658323 6 0.5532207982260293 1.963255898676319 7 0.38336030468483523 1.5872025158037806 8 0.26919638959861214 1.2737564012453346 9 0.19324854503390965 1.0286932825872255 >10 1.0114050406243813 10.545026115309073 >50 0.06113513009092826 2.4674154440202067 >100 0.029447629214144472 3.2104890607367444 >500 0.0020231757509321185 0.8301155050344119 >1k 0.0010442197424065285 1.0205730684698717 >5k 6.526373390040803E-5 0.22500550006092826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5829 0.22356237770037196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4573 0.17539041914973424 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3122 0.11973953391328895 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.670694036725749E-5 2 0.0 0.0 0.0 0.0 7.670694036725749E-5 3 0.0 0.0 0.0 0.0 7.670694036725749E-5 4 0.0 0.0 0.0 0.0 1.1506041055088623E-4 5 0.0 0.0 0.0 0.0 1.1506041055088623E-4 6 0.0 0.0 0.0 0.0 1.1506041055088623E-4 7 0.0 0.0 0.0 0.0 1.1506041055088623E-4 8 0.0 0.0 0.0 0.0 1.1506041055088623E-4 9 0.0 0.0 0.0 0.0 1.1506041055088623E-4 10-11 0.0 0.0 0.0 0.0 1.1506041055088623E-4 12-13 0.0 0.0 0.0 0.0 1.5341388073451497E-4 14-15 0.0 0.0 0.0 1.917673509181437E-5 1.7259061582632936E-4 16-17 0.0 0.0 0.0 3.835347018362874E-5 1.9176735091814371E-4 18-19 0.0 0.0 0.0 3.835347018362874E-5 1.9176735091814371E-4 20-21 0.0 0.0 0.0 1.3423714564270059E-4 1.9176735091814371E-4 22-23 0.0 0.0 0.0 1.9176735091814371E-4 2.3012082110177246E-4 24-25 0.0 0.0 0.0 3.8353470183628743E-4 2.3012082110177246E-4 26-27 0.0 0.0 0.0 7.478926685807605E-4 2.876510263772156E-4 28-29 0.0 0.0 0.0 0.0026847429128540124 3.0682776146902994E-4 30-31 0.0 0.0 0.0 0.01022119980393706 3.0682776146902994E-4 32-33 0.0 0.0 0.0 0.02719261036019278 3.0682776146902994E-4 34-35 0.0 0.0 0.0 0.05252507741647956 3.0682776146902994E-4 36-37 0.0 0.0 0.0 0.09745616773660065 3.0682776146902994E-4 38-39 0.0 0.0 0.0 0.16735536714626403 3.2600449656084433E-4 40-41 0.0 0.0 0.0 0.24379383322223613 3.451812316526587E-4 42-43 0.0 0.0 0.0 0.32270609812505224 3.451812316526587E-4 44-45 0.0 0.0 0.0 0.4217731116093653 3.6435796674447304E-4 46-47 0.0 0.0 0.0 0.5389046095501675 4.410649071117306E-4 48-49 0.0 0.0 0.0 0.665298470540316 4.794183772953593E-4 50-51 0.0 0.0 0.0 0.798672663103885 4.985951123871737E-4 52-53 0.0 0.0 0.0 0.9295730568406099 5.17771847478988E-4 54-55 0.0 0.0 0.0 1.0766969684650096 5.369485825708025E-4 56-57 0.0 0.0 0.0 1.2529503406938756 5.369485825708025E-4 58-59 0.0 0.0 0.0 1.4435479107714186 5.369485825708025E-4 60-61 0.0 0.0 0.0 1.6473007211219464 5.561253176626168E-4 62-63 0.0 0.0 0.0 1.8470839473084686 6.136555229380599E-4 64-65 0.0 0.0 0.0 2.0588526329273744 6.136555229380599E-4 66-67 0.0 0.0 0.0 2.303145061261998 6.136555229380599E-4 68-69 0.0 0.0 0.0 2.580632418040552 6.136555229380599E-4 70-71 0.0 0.0 0.0 2.8705654758936934 7.095391983971318E-4 72-73 0.0 0.0 0.0 3.167095330618419 7.67069403672575E-4 74-75 0.0 0.0 0.0 3.4671920580702222 8.054228738562036E-4 76-77 0.0 0.0 0.0 3.7866764646998496 8.24599608948018E-4 78-79 0.0 0.0 0.0 4.148311335061285 8.437763440398324E-4 80-81 0.0 0.0 0.0 4.531059790758809 8.821298142234612E-4 82-83 0.0 0.0 0.0 4.925160873630685 9.971902247743474E-4 84-85 0.0 0.0 0.0 5.323384954547302 9.971902247743474E-4 86-87 0.0 0.0 0.0 5.7317918817976725 9.971902247743474E-4 88-89 0.0 0.0 0.0 6.171936305624996 0.0010163669598661617 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4540 0.0 45.148605 1 GTATCAA 7595 0.0 39.415188 1 ATCAACG 9405 0.0 31.38125 3 TCAACGC 9545 0.0 30.970762 4 TATCAAC 9705 0.0 30.558111 2 CAACGCA 9820 0.0 30.055056 5 AACGCAG 10225 0.0 28.860735 6 GTACATG 9120 0.0 26.499939 1 TACATGG 9005 0.0 25.861267 2 ACGCAGA 11820 0.0 24.87772 7 CGCAGAG 11965 0.0 24.615936 8 ACATGGG 9445 0.0 24.052673 3 GGACCGA 975 0.0 23.394604 6 ACCGAGT 995 0.0 22.916882 8 AGGACCG 1145 0.0 22.414328 5 GCAGAGT 12880 0.0 21.760733 9 GAGTACT 6875 0.0 21.280308 12-13 GTACTTT 7300 0.0 19.947035 14-15 AGAGTAC 11145 0.0 19.818779 10-11 GTGTAGC 1675 0.0 19.636587 1 >>END_MODULE