FastQCFastQC Report
Thu 26 May 2016
SRR938437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607326
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58350.2237924985214737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49310.18912096147547336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34460.13216605825278466No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47400.042.2514881
GTATCAA76550.036.7265851
ATCAACG97050.028.8018153
TCAACGC97750.028.5955584
TATCAAC100300.028.0301112
CAACGCA100700.027.7578545
AACGCAG104500.026.9061686
ACGCAGA119100.023.4084577
TACATGG88300.022.8963622
GTACATG89950.022.740791
CGCAGAG122300.022.6794668
ACATGGG90600.021.092883
GGACCGA9750.020.4593166
AGAGTAC113600.020.40271410-11
GAGTACT70250.020.28249512-13
GCAGAGT134650.020.2465939
AGTACTT75100.019.79478812-13
TAAGGTG12550.019.696895
GTACTTT74100.019.10048914-15
GTAAGGT11500.019.0150764
Produced by FastQC (version 0.11.5)