##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938437_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2607326 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75679450901038 31.0 31.0 34.0 30.0 34.0 2 31.986963655484583 31.0 31.0 34.0 30.0 34.0 3 32.06143688974835 33.0 31.0 34.0 30.0 34.0 4 35.739637084123736 37.0 35.0 37.0 33.0 37.0 5 35.60691259934508 37.0 35.0 37.0 33.0 37.0 6 35.64331924738218 37.0 35.0 37.0 33.0 37.0 7 35.56843639805686 37.0 35.0 37.0 33.0 37.0 8 35.55350270737146 37.0 35.0 37.0 33.0 37.0 9 37.112048129002666 39.0 37.0 39.0 33.0 39.0 10-11 37.04492437846284 39.0 37.0 39.0 33.0 39.0 12-13 36.93671389768675 39.0 37.0 39.0 33.0 39.0 14-15 37.98399164508005 40.0 37.0 41.0 33.0 41.0 16-17 37.8967390345511 40.0 37.0 41.0 33.0 41.0 18-19 37.88614082013527 40.0 37.0 41.0 33.0 41.0 20-21 37.868941973500824 40.0 37.0 41.0 32.5 41.0 22-23 37.89973041345808 40.0 37.0 41.0 32.5 41.0 24-25 37.83590659549285 40.0 37.0 41.0 32.0 41.0 26-27 37.664382014370275 40.0 37.0 41.0 32.0 41.0 28-29 37.53544186649464 39.5 36.5 41.0 32.0 41.0 30-31 37.30088642540289 39.0 36.0 41.0 31.0 41.0 32-33 37.096854401789415 39.0 36.0 41.0 31.0 41.0 34-35 36.93990356403457 39.0 36.0 40.5 30.5 41.0 36-37 36.96639334705365 39.0 36.0 41.0 31.0 41.0 38-39 36.82278568157568 39.0 35.0 41.0 30.0 41.0 40-41 36.65224409989392 39.0 35.0 40.0 30.0 41.0 42-43 36.43925826689873 39.0 35.0 40.0 30.0 41.0 44-45 36.17813556877813 38.5 35.0 40.0 30.0 41.0 46-47 35.849246124190074 38.0 35.0 40.0 28.5 41.0 48-49 35.47769764885557 38.0 34.0 40.0 27.5 41.0 50-51 35.11579756424781 38.0 34.0 40.0 26.5 41.0 52-53 34.59039030792467 38.0 33.0 40.0 25.5 41.0 54-55 34.26143144355558 37.5 33.0 40.0 24.0 41.0 56-57 33.897102625448454 37.0 33.0 40.0 23.5 41.0 58-59 33.434220346822755 36.5 32.0 39.0 22.5 41.0 60-61 33.01168553529555 36.0 31.0 39.0 22.0 40.0 62-63 32.52022340129312 35.5 31.0 39.0 20.5 40.0 64-65 32.00828550016377 35.0 30.5 38.5 18.5 40.0 66-67 31.505986593160962 35.0 30.0 38.0 17.5 40.0 68-69 30.9322447595736 34.0 29.0 37.5 13.5 39.5 70-71 30.360685046672337 34.0 29.0 37.0 9.5 39.0 72-73 29.75696153837303 34.0 28.0 36.0 7.5 39.0 74-75 29.131168676260657 33.5 27.5 36.0 6.5 38.5 76-77 27.864880341008373 31.5 26.0 34.5 2.0 36.5 78-79 28.48814091525187 33.0 27.0 35.0 2.0 37.0 80-81 28.17184694203947 33.0 26.0 35.0 2.0 37.0 82-83 27.660572555944288 33.0 26.0 35.0 2.0 36.0 84-85 27.053154841396896 32.0 25.5 35.0 2.0 36.0 86-87 26.395364829714428 32.0 24.5 34.5 2.0 35.0 88-89 25.629212649281293 31.5 22.0 34.0 2.0 35.0 90-91 25.0721210159374 31.0 19.5 34.0 2.0 35.0 92-93 24.27588475702693 31.0 16.5 34.0 2.0 35.0 94-95 23.459182702891773 30.5 4.5 34.0 2.0 35.0 96-97 22.449098233209043 30.0 2.0 34.0 2.0 35.0 98-99 21.268388763046893 29.5 2.0 34.0 2.0 35.0 100-101 18.147415781532498 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28717406387974975 1101 2 0.3307397276665398 1101 3 0.2759289564311871 1101 4 0.218617276410221 1101 5 0.1744864920520044 1101 6 0.26934532375085496 1101 7 0.25090013459423943 1101 8 0.286920494876199 1101 9 0.30531945944515115 1101 10-11 0.3028130800342339 1101 12-13 0.32102210586334223 1101 14-15 0.3686194783384096 1101 16-17 0.45162053948085656 1101 18-19 0.549039532819279 1101 20-21 0.6796741442752818 1101 22-23 0.5740211111795048 1101 24-25 0.5061539354458517 1101 26-27 0.5360603391538206 1101 28-29 0.5070461915271665 1101 30-31 0.5594143940338725 1101 32-33 0.43950795134468734 1101 34-35 0.3704084260772973 1101 36-37 0.5899330556130664 1101 38-39 0.7307959923458824 1101 40-41 0.7690825413613211 1101 42-43 0.734969136600661 1101 44-45 0.8211094399010719 1101 46-47 0.9370650918183543 1101 48-49 0.9613892675740203 1101 50-51 0.9463999822384963 1101 52-53 1.0460442780734596 1101 54-55 1.131808685803108 1101 56-57 1.2877865775934012 1101 58-59 1.313963765998139 1101 60-61 1.4676765575864508 1101 62-63 1.6404635210037313 1101 64-65 1.5026117360687294 1101 66-67 1.5267206608386026 1101 68-69 1.5859301052427028 1101 70-71 1.8523604452718594 1101 72-73 1.7464920649599947 1101 74-75 1.7561416435531036 1101 76-77 1.7915289629501885 1101 78-79 2.107287430973134 1101 80-81 2.3760316014981093 1101 82-83 2.4699589907767283 1101 84-85 2.594184695789835 1101 86-87 2.6901578059003235 1101 88-89 2.849741858635081 1101 90-91 3.1213590033041463 1101 92-93 3.3365534427210157 1101 94-95 3.2237134366993203 1101 96-97 3.618860677571906 1101 98-99 3.988130590098841 1101 100-101 4.36917822581659 1102 1 0.3136895786273506 1102 2 0.3423088627882791 1102 3 0.35883576406110507 1102 4 0.17497280905791257 1102 5 0.1554057098817836 1102 6 0.18076582415440612 1102 7 0.19059219702082686 1102 8 0.2937439072416552 1102 9 0.35616192262610014 1102 10-11 0.3266305949576349 1102 12-13 0.317882596997201 1102 14-15 0.5125424360094826 1102 16-17 0.5328187470740104 1102 18-19 0.5122249706124506 1102 20-21 0.5164206821251938 1102 22-23 0.5681301619150858 1102 24-25 0.5300613504004232 1102 26-27 0.4876187062496129 1102 28-29 0.43988387602200874 1102 30-31 0.42673393470465015 1102 32-33 0.4133330578262786 1102 34-35 0.6491155038068612 1102 36-37 0.7440097223596425 1102 38-39 0.8297339733110434 1102 40-41 0.6454245490487764 1102 42-43 0.6387391443520372 1102 44-45 0.7023844620764095 1102 46-47 0.6498005300110847 1102 48-49 0.9080640651990706 1102 50-51 1.0015831185908723 1102 52-53 1.0104648424059306 1102 54-55 0.9667117735695072 1102 56-57 1.1750009951610636 1102 58-59 1.1916867380532068 1102 60-61 1.2156408213251737 1102 62-63 1.2990591626502237 1102 64-65 1.230966805231823 1102 66-67 1.3007154786012443 1102 68-69 1.4825960127535538 1102 70-71 1.6571280437024036 1102 72-73 1.7012169109312758 1102 74-75 1.6214234501841318 1102 76-77 1.5659343241884898 1102 78-79 1.7834854987217952 1102 80-81 2.0775120552335586 1102 82-83 2.0969487781331893 1102 84-85 2.313984278653688 1102 86-87 2.3606432261130443 1102 88-89 2.635667908867223 1102 90-91 2.819164543543401 1102 92-93 2.926659245068805 1102 94-95 3.0913681047084935 1102 96-97 3.3771010143174465 1102 98-99 3.6170087775543927 1102 100-101 3.963090795039612 1103 1 0.24349372516442358 1103 2 0.25225489820102354 1103 3 0.29480190157038777 1103 4 0.1946173130224409 1103 5 0.13672883356032628 1103 6 0.177955994643618 1103 7 0.18501186985651685 1103 8 0.1655437969845437 1103 9 0.2682293201618151 1103 10-11 0.3740696113925992 1103 12-13 0.4652598656139588 1103 14-15 0.5974824597237145 1103 16-17 0.5953218648431857 1103 18-19 0.43439457981367013 1103 20-21 0.5110689913669688 1103 22-23 0.41323299393671675 1103 24-25 0.48981638647700976 1103 26-27 0.42811999876479234 1103 28-29 0.4301853754931102 1103 30-31 0.6243376652833419 1103 32-33 0.6057627305468571 1103 34-35 0.5169102593034083 1103 36-37 0.5969266432444158 1103 38-39 0.6956946811054081 1103 40-41 0.6821654601920315 1103 42-43 0.7343983782569978 1103 44-45 0.788276124873839 1103 46-47 0.7684089433191943 1103 48-49 0.8831185542110873 1103 50-51 0.9114127572877919 1103 52-53 1.09931374923611 1103 54-55 0.9004518238887016 1103 56-57 1.1040154667263735 1103 58-59 1.207839980085467 1103 60-61 1.3107500487707497 1103 62-63 1.4383372663814384 1103 64-65 1.3498367913793317 1103 66-67 1.318863613247938 1103 68-69 1.4358729640293717 1103 70-71 1.545406542670822 1103 72-73 1.3450629462161068 1103 74-75 1.5965025615783475 1103 76-77 1.6058569587450116 1103 78-79 1.8775366032754661 1103 80-81 2.142707486598507 1103 82-83 2.278603920245903 1103 84-85 2.339770308233806 1103 86-87 2.442067525398418 1103 88-89 2.6046537800966405 1103 90-91 2.8483518194637334 1103 92-93 2.9600216136268536 1103 94-95 3.0158557353190396 1103 96-97 3.3243542756638895 1103 98-99 3.7692761472060567 1103 100-101 4.101683469209391 1104 1 0.26881621894773033 1104 2 0.2783696307892001 1104 3 0.21176827313825441 1104 4 0.1528192748506214 1104 5 0.1419697623720353 1104 6 0.1681710665820475 1104 7 0.18926608582540894 1104 8 0.276891519031949 1104 9 0.3622316853115706 1104 10-11 0.3287121277046694 1104 12-13 0.34229744768323656 1104 14-15 0.41767270499233433 1104 16-17 0.4062889359623867 1104 18-19 0.36334369824943735 1104 20-21 0.4229551335264077 1104 22-23 0.45137519779486723 1104 24-25 0.5079572979541496 1104 26-27 0.48734875575336645 1104 28-29 0.5276689184553689 1104 30-31 0.559562773994486 1104 32-33 0.4660393802758236 1104 34-35 0.5098468882185685 1104 36-37 0.6518521416438929 1104 38-39 0.6308392858928258 1104 40-41 0.7017804262724354 1104 42-43 0.7074335735854262 1104 44-45 0.5986344043476848 1104 46-47 0.6841562664866174 1104 48-49 0.7019477896582345 1104 50-51 0.7423239815088039 1104 52-53 0.9363637060847694 1104 54-55 0.9415129118699284 1104 56-57 1.0664979393874248 1104 58-59 1.1030093745294067 1104 60-61 1.0509308834082844 1104 62-63 0.9597836196153864 1104 64-65 1.1018523968027623 1104 66-67 1.3600545562172321 1104 68-69 1.520776689356154 1104 70-71 1.5314223788104862 1104 72-73 1.7497227816673195 1104 74-75 1.7525908018982896 1104 76-77 1.5528865336040987 1104 78-79 1.769882962616606 1104 80-81 2.0326997230591957 1104 82-83 2.2395205761684345 1104 84-85 2.358779567110364 1104 86-87 2.508876837203996 1104 88-89 2.610057798753811 1104 90-91 2.733036040509621 1104 92-93 3.0372614271045073 1104 94-95 3.1806645400424323 1104 96-97 3.499948832611622 1104 98-99 3.7719614619949056 1104 100-101 4.074909128801792 1105 1 0.25565054465944925 1105 2 0.2276343717451681 1105 3 0.21178284817437287 1105 4 0.11480978900490157 1105 5 0.09458455329534843 1105 6 0.1457951472506025 1105 7 0.20019500734929352 1105 8 0.17732120287438846 1105 9 0.24527629552210328 1105 10-11 0.2919103823514533 1105 12-13 0.3243592587919508 1105 14-15 0.4558473009693884 1105 16-17 0.42266619737493727 1105 18-19 0.41734494316364135 1105 20-21 0.3975520969722197 1105 22-23 0.29931450852226504 1105 24-25 0.21079873805016547 1105 26-27 0.36020136709927897 1105 28-29 0.3803847924069217 1105 30-31 0.42927291922072897 1105 32-33 0.4700977917205549 1105 34-35 0.5030844395182754 1105 36-37 0.5912318428291528 1105 38-39 0.5567753565455362 1105 40-41 0.5421789224820444 1105 42-43 0.617507220212353 1105 44-45 0.5632362055277085 1105 46-47 0.7087693802502244 1105 48-49 0.566751742172471 1105 50-51 0.7671726514116131 1105 52-53 0.7913902385394067 1105 54-55 0.7026943201305329 1105 56-57 0.7693507892339326 1105 58-59 1.1259591675050018 1105 60-61 1.0005125945940776 1105 62-63 1.252162966288651 1105 64-65 1.3064586676605288 1105 66-67 1.288865663160319 1105 68-69 1.4013849865856045 1105 70-71 1.442733504892498 1105 72-73 1.4481351774558142 1105 74-75 1.6392944822862994 1105 76-77 1.535931570760134 1105 78-79 1.9210316079626928 1105 80-81 2.235070124488079 1105 82-83 2.324778795752689 1105 84-85 2.442478110607869 1105 86-87 2.5717403665769645 1105 88-89 2.6149366080646352 1105 90-91 2.732306699813588 1105 92-93 2.8889717707867995 1105 94-95 2.8261369449351683 1105 96-97 3.080761150902145 1105 98-99 3.4263851474002607 1105 100-101 3.7784653345577226 1106 1 0.1895463630432026 1106 2 0.23372523014428026 1106 3 0.21702529416163685 1106 4 0.1172841823757409 1106 5 0.08748017057394009 1106 6 0.10796566614763492 1106 7 0.055523837145763366 1106 8 -0.015645486797495778 1106 9 0.20016132278556853 1106 10-11 0.2132237019121348 1106 12-13 0.11738376619855728 1106 14-15 0.14739454240071126 1106 16-17 0.1551903299946531 1106 18-19 0.3215111908612158 1106 20-21 0.45377041719562783 1106 22-23 0.3082516905818551 1106 24-25 0.180508700982827 1106 26-27 0.2695237912392159 1106 28-29 0.384095811141691 1106 30-31 0.4474206606726199 1106 32-33 0.3836939862623794 1106 34-35 0.3334168115079805 1106 36-37 0.3975841379418412 1106 38-39 0.34299599633418865 1106 40-41 0.3262319685396946 1106 42-43 0.41782150416369745 1106 44-45 0.05550079822118903 1106 46-47 -0.14539766729743775 1106 48-49 0.36403887921192535 1106 50-51 0.2780731797697342 1106 52-53 0.45731799607970913 1106 54-55 0.6000049956153504 1106 56-57 0.3572511713337363 1106 58-59 0.7967211618283585 1106 60-61 0.7296913542322727 1106 62-63 0.7955491201980891 1106 64-65 0.5952428269723811 1106 66-67 0.6061716195296292 1106 68-69 0.705028646516773 1106 70-71 0.7706143363709366 1106 72-73 1.3471552468370938 1106 74-75 1.5561563504618654 1106 76-77 1.5073503562677644 1106 78-79 1.740068382784326 1106 80-81 1.778041213335424 1106 82-83 2.2054440196421545 1106 84-85 2.1727806596158246 1106 86-87 2.2388576154583184 1106 88-89 2.5609393001314977 1106 90-91 2.6791546620241 1106 92-93 2.422300952517311 1106 94-95 2.9697188907190437 1106 96-97 3.476432990694459 1106 98-99 3.8268955088867926 1106 100-101 3.8989927442775 1107 1 0.22825296092608482 1107 2 0.09192940085072365 1107 3 0.17215065691448928 1107 4 0.11886769301814581 1107 5 0.0672772771546164 1107 6 0.06060125657305804 1107 7 0.009844553132481337 1107 8 0.1405650460783079 1107 9 0.16832097043962335 1107 10-11 0.19503001257086083 1107 12-13 0.1580800586465685 1107 14-15 0.2826658528553807 1107 16-17 0.19692421141431282 1107 18-19 0.3212844530944352 1107 20-21 0.2734448165232308 1107 22-23 0.2066472502994401 1107 24-25 0.27818083693118467 1107 26-27 0.278159473766074 1107 28-29 0.268212820238233 1107 30-31 0.22064256504374669 1107 32-33 0.2327628792471188 1107 34-35 0.24979049078978477 1107 36-37 0.24580316069586416 1107 38-39 0.41011271919928305 1107 40-41 0.41525160103837777 1107 42-43 0.45527294408984176 1107 44-45 0.570773713557351 1107 46-47 0.5052496211146575 1107 48-49 0.5767053925600152 1107 50-51 0.5587605405834921 1107 52-53 0.7435430942201364 1107 54-55 0.6354684644315682 1107 56-57 0.5491719028729207 1107 58-59 0.748758299668367 1107 60-61 0.6033976598171478 1107 62-63 0.8237420898244707 1107 64-65 0.8736860422945298 1107 66-67 0.9983386810743546 1107 68-69 1.0302731841289052 1107 70-71 1.0744605920335246 1107 72-73 1.2788205237094807 1107 74-75 1.2479811889534282 1107 76-77 1.2964381135037968 1107 78-79 1.6055324361965475 1107 80-81 1.7967082324142147 1107 82-83 1.9704655173330003 1107 84-85 2.0962668317142708 1107 86-87 2.240827180066656 1107 88-89 2.347360596412475 1107 90-91 2.46994574450839 1107 92-93 2.643926951156196 1107 94-95 2.948350734766475 1107 96-97 3.2128538871338286 1107 98-99 3.3385690224963014 1107 100-101 3.531374716550001 1108 1 0.09343095453073502 1108 2 0.13274237080354112 1108 3 0.11436406490394546 1108 4 0.15166124729261554 1108 5 0.04073962987938984 1108 6 0.08209468900294326 1108 7 0.11703521110516135 1108 8 0.06267985808129595 1108 9 0.1196431252176069 1108 10-11 0.14120823619837353 1108 12-13 0.22252440244773197 1108 14-15 0.31842725835471697 1108 16-17 0.362256427607214 1108 18-19 0.28411510103985904 1108 20-21 0.19949901849284402 1108 22-23 0.15003262283121188 1108 24-25 0.3281812143434877 1108 26-27 0.31242235375571425 1108 28-29 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70-71 0.7995470742442201 2316 72-73 0.9408658721679899 2316 74-75 0.9549458528693222 2316 76-77 1.0462578970706602 2316 78-79 1.2025866874191102 2316 80-81 1.4796549428714698 2316 82-83 1.6509936424606835 2316 84-85 1.7734024736309486 2316 86-87 1.9777833793608686 2316 88-89 2.2350375760441743 2316 90-91 2.280139511167814 2316 92-93 2.339090148352714 2316 94-95 2.8415358275206266 2316 96-97 2.9592038721108658 2316 98-99 3.379420773903121 2316 100-101 3.899654469734614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 10.0 4 39.0 5 74.0 6 159.0 7 443.0 8 1002.0 9 1903.0 10 3059.0 11 4812.0 12 6899.0 13 9978.0 14 13206.0 15 16988.0 16 20775.0 17 25715.0 18 25418.0 19 23927.0 20 24653.0 21 26567.0 22 29519.0 23 33296.0 24 38218.0 25 44844.0 26 53206.0 27 63571.0 28 75045.0 29 88805.0 30 104446.0 31 122605.0 32 145581.0 33 176004.0 34 217115.0 35 271886.0 36 342007.0 37 379389.0 38 204247.0 39 11910.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30705504954505 19.30389165289776 14.781400393660322 23.60765290389687 2 16.17619578776357 20.319595544264395 39.10594439577786 24.39826427219417 3 19.68585415544827 23.644242142518536 29.445630846032177 27.22427285600102 4 13.578509169931186 16.54453643311193 35.91000895169994 33.96694544525694 5 14.097683959325034 37.117040541749354 34.094712461517375 14.69056303740824 6 33.51103774518415 35.6933885521028 16.627801816880588 14.167771885832458 7 29.634997694956443 31.817041674113632 20.05100244465019 18.496958186279734 8 26.6047667226883 35.6889395495615 18.992638434932953 18.71365529281724 9 26.182533369436733 16.737032499963565 19.601001178985676 37.47943295161403 10-11 25.385471552080563 26.46086450255933 26.95192315805542 21.201740787304697 12-13 26.107015386645166 23.359161838603995 27.435407003190242 23.098415771560596 14-15 22.721918931503005 25.018486372628505 25.51748419645261 26.742110499415876 16-17 22.699175323684113 28.26002578887335 26.248865696119317 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22.799368974341665 26.279886651651747 27.818366482764546 23.102377891242046 44-45 22.790319571059356 26.49347849975098 27.908277673059516 22.807924256130143 46-47 23.061096357798263 26.6685881769968 27.388061476057445 22.882253989147493 48-49 22.924233486721647 26.64093404507146 27.415654966045672 23.019177502161217 50-51 23.059947240966416 26.797262789539932 27.36299565148355 22.7797943180101 52-53 23.22676565953011 26.663926950446548 27.226073762927992 22.883233627095343 54-55 22.923451636552475 26.74282516701501 27.36982781781561 22.9638953786169 56-57 22.882274790340755 26.83686274750453 27.34192041961765 22.938942042537068 58-59 22.973076631000495 26.574141476746675 27.21652374885227 23.236258143400555 60-61 22.779046425341516 26.741611904303493 27.42810066712026 23.051241003234733 62-63 22.74565972954667 26.797281966275026 27.4458199703451 23.011238333833205 64-65 22.557919492997808 26.704198094139358 27.413411288039928 23.324471124822903 66-67 22.54666330945378 26.763174391804 27.292970966564727 23.397191332177485 68-69 22.793885833817225 26.566684130306882 27.39924336157943 23.240186674296464 70-71 22.726482994454855 26.587009065993282 27.147928567428853 23.53857937212301 72-73 22.75872781490608 26.658778633273567 27.039609561523786 23.542883990296566 74-75 22.567335269927888 26.977581629608267 26.937272132445273 23.517810968018576 76-77 22.697852128962776 26.62379004389938 27.153144639373828 23.525213187764017 78-79 22.726943236097057 26.72763206442156 27.15782376273623 23.387600936745155 80-81 22.72157810752819 26.831460053606655 27.022632962397676 23.424328876467477 82-83 22.702894472149804 26.80048977916789 27.05957030219988 23.437045446482422 84-85 22.73577276232415 26.719497117319158 26.974233169307325 23.570496951049368 86-87 22.71988435506432 26.78801483992389 26.923385230110487 23.568715574901304 88-89 22.66471293939687 26.876253200599542 26.7425145474824 23.71651931252119 90-91 22.629575969800207 26.762602344760534 26.906908576967957 23.7009131084713 92-93 22.58508366458074 26.597715811969092 26.95118274884977 23.866017774600394 94-95 22.466293052729117 26.503571091608798 27.040730618265613 23.989405237396475 96-97 22.587739316065576 26.318343007356965 26.92162391661035 24.172293759967108 98-99 22.37234622751432 26.52316971487263 26.76811415220038 24.33636990541267 100-101 22.544817246580866 26.29375628760819 26.93687097549928 24.224555490311666 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 303.0 1 209.5 2 182.0 3 258.0 4 222.5 5 204.0 6 387.5 7 917.0 8 1491.5 9 1689.5 10 1500.5 11 1298.0 12 1359.0 13 1534.0 14 1717.5 15 1855.0 16 2028.5 17 2197.5 18 2383.0 19 2603.0 20 2950.0 21 3577.0 22 4221.0 23 5223.5 24 6651.0 25 8339.0 26 10901.5 27 14150.0 28 17339.5 29 20973.0 30 25305.0 31 30431.5 32 37320.5 33 41736.5 34 44321.5 35 48660.0 36 54515.0 37 60127.0 38 64026.0 39 68020.5 40 72670.5 41 78198.5 42 82431.5 43 86758.0 44 91423.5 45 95556.0 46 98446.5 47 102119.0 48 105475.5 49 104847.0 50 103744.5 51 100854.0 52 97131.5 53 96204.5 54 95779.0 55 90278.0 56 82345.5 57 75242.5 58 68583.5 59 60797.5 60 50057.5 61 40740.0 62 33492.5 63 25705.0 64 19297.5 65 14731.0 66 10696.0 67 8048.5 68 6078.5 69 4497.0 70 3349.5 71 2416.0 72 1804.5 73 1403.0 74 1054.5 75 762.5 76 537.0 77 350.5 78 206.5 79 107.5 80 61.0 81 30.5 82 13.5 83 6.5 84 6.0 85 4.5 86 1.0 87 0.5 88 0.0 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.670694036725749E-5 2 0.04437496500245846 3 7.670694036725749E-5 4 0.0 5 0.09891359960357853 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011122506353252336 26-27 0.012426524339495714 28-29 0.0 30-31 0.0 32-33 4.410649071117306E-4 34-35 0.015245504397992427 36-37 0.0033751053761593296 38-39 0.0125415847500466 40-41 0.006980331573420432 42-43 0.005446192766075282 44-45 0.0025121522970276825 46-47 7.670694036725749E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.917673509181437E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7259061582632936E-4 68-69 1.5341388073451497E-4 70-71 0.0 72-73 3.835347018362874E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.917673509181437E-5 82-83 0.0010930739002334192 84-85 0.004506532746576378 86-87 0.0013423714564270062 88-89 7.670694036725749E-5 90-91 9.013065493152754E-4 92-93 3.8353470183628743E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 5.753020527544312E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2607326.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.985162349186744 #Duplication Level Percentage of deduplicated Percentage of total 1 75.29602531206817 42.154602013446116 2 13.989559558837817 15.664155261903048 3 4.651314666159076 7.812138201662076 4 2.071723349323782 4.639430722179714 5 1.1088772985661777 3.1040337792777537 6 0.6543826277745346 2.1981430592666857 7 0.4218993115235545 1.653407101545942 8 0.30515196025233227 1.366718562871952 9 0.22891017140198835 1.1534015798388437 >10 1.1635238636790646 11.618777073611795 >50 0.07115470632239995 2.7354488710178497 >100 0.033765622172851624 3.5470399422350924 >500 0.0025431003884131822 1.0114075251952852 >1k 0.0010997190868728835 1.1167656823942698 >5k 6.873244292955522E-5 0.22453062355353054 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5835 0.2237924985214737 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4931 0.18912096147547336 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3446 0.13216605825278466 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 7.670694036725749E-5 10-11 0.0 0.0 0.0 0.0 7.670694036725749E-5 12-13 0.0 0.0 0.0 0.0 1.7259061582632936E-4 14-15 0.0 0.0 0.0 1.917673509181437E-5 3.6435796674447304E-4 16-17 0.0 0.0 0.0 3.835347018362874E-5 4.602416422035449E-4 18-19 0.0 0.0 0.0 3.835347018362874E-5 4.602416422035449E-4 20-21 0.0 0.0 0.0 1.3423714564270059E-4 4.794183772953593E-4 22-23 0.0 0.0 0.0 1.9176735091814371E-4 5.17771847478988E-4 24-25 0.0 0.0 0.0 3.8353470183628743E-4 5.753020527544311E-4 26-27 0.0 0.0 0.0 7.287159334889461E-4 5.753020527544311E-4 28-29 0.0 0.0 0.0 0.0026655661777621976 5.753020527544311E-4 30-31 0.0 0.0 0.0 0.010317083479396132 5.753020527544311E-4 32-33 0.0 0.0 0.0 0.02730767077074367 6.136555229380599E-4 34-35 3.835347018362874E-5 0.0 0.0 0.052946965588499484 6.136555229380599E-4 36-37 3.835347018362874E-5 0.0 0.0 0.0986067718421095 6.136555229380599E-4 38-39 3.835347018362874E-5 0.0 0.0 0.16871691533778285 6.136555229380599E-4 40-41 3.835347018362874E-5 0.0 0.0 0.24538550223485672 6.136555229380599E-4 42-43 3.835347018362874E-5 0.0 0.0 0.3246237716342337 6.136555229380599E-4 44-45 3.835347018362874E-5 0.0 0.0 0.4246879753433211 6.136555229380599E-4 46-47 3.835347018362874E-5 0.0 0.0 0.5421646545157759 6.520089931216887E-4 48-49 3.835347018362874E-5 0.0 0.0 0.6693064081745053 7.095391983971318E-4 50-51 3.835347018362874E-5 0.0 0.0 0.8033901399364713 7.670694036725749E-4 52-53 3.835347018362874E-5 0.0 0.0 0.9347124218452161 8.054228738562036E-4 54-55 3.835347018362874E-5 0.0 0.0 1.0816637428537896 8.437763440398324E-4 56-57 3.835347018362874E-5 0.0 0.0 1.257878761612472 8.821298142234612E-4 58-59 3.835347018362874E-5 0.0 0.0 1.4493201080340548 8.821298142234612E-4 60-61 3.835347018362874E-5 0.0 0.0 1.6546262339270195 9.971902247743474E-4 62-63 3.835347018362874E-5 0.0 0.0 1.8550614691066634 0.001035543694957976 64-65 3.835347018362874E-5 0.0 0.0 2.0671753359572222 0.001073897165141605 66-67 3.835347018362874E-5 0.0 0.0 2.313366261065935 0.001073897165141605 68-69 3.835347018362874E-5 0.0 0.0 2.5938068350486283 0.001073897165141605 70-71 3.835347018362874E-5 0.0 0.0 2.8871917052182967 0.001073897165141605 72-73 3.835347018362874E-5 0.0 0.0 3.1872500791999157 0.0011506041055088622 74-75 3.835347018362874E-5 0.0 0.0 3.4936367757618343 0.0011889575756924911 76-77 3.835347018362874E-5 0.0 0.0 3.821328825010758 0.0011889575756924911 78-79 3.835347018362874E-5 0.0 0.0 4.192321942096999 0.0011889575756924911 80-81 3.835347018362874E-5 0.0 0.0 4.58678738293562 0.0012081343107843054 82-83 3.835347018362874E-5 0.0 0.0 4.9964216212318675 0.0012273110458761198 84-85 3.835347018362874E-5 0.0 0.0 5.412115707817128 0.0012273110458761198 86-87 3.835347018362874E-5 0.0 0.0 5.8389706542258235 0.0012273110458761198 88-89 3.835347018362874E-5 0.0 0.0 6.30208880669314 0.0012273110458761198 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4740 0.0 42.251488 1 GTATCAA 7655 0.0 36.726585 1 ATCAACG 9705 0.0 28.801815 3 TCAACGC 9775 0.0 28.595558 4 TATCAAC 10030 0.0 28.030111 2 CAACGCA 10070 0.0 27.757854 5 AACGCAG 10450 0.0 26.906168 6 ACGCAGA 11910 0.0 23.408457 7 TACATGG 8830 0.0 22.896362 2 GTACATG 8995 0.0 22.74079 1 CGCAGAG 12230 0.0 22.679466 8 ACATGGG 9060 0.0 21.09288 3 GGACCGA 975 0.0 20.459316 6 AGAGTAC 11360 0.0 20.402714 10-11 GAGTACT 7025 0.0 20.282495 12-13 GCAGAGT 13465 0.0 20.246593 9 AGTACTT 7510 0.0 19.794788 12-13 TAAGGTG 1255 0.0 19.69689 5 GTACTTT 7410 0.0 19.100489 14-15 GTAAGGT 1150 0.0 19.015076 4 >>END_MODULE