FastQCFastQC Report
Thu 26 May 2016
SRR938436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614933
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55800.21338978857202076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44350.1696028158274036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27580.10547115356301673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42300.041.6037181
GTATCAA72700.040.0167271
ATCAACG88900.032.076613
TCAACGC90050.031.666974
TATCAAC92100.031.3749432
CAACGCA92850.030.609645
AACGCAG96950.029.4047666
ACGCAGA110350.025.6153777
GTACATG90450.025.3619481
CGCAGAG112700.024.9969438
TACATGG89650.024.9694862
ACATGGG94150.023.1702823
GGACCGA9550.022.885946
GCAGAGT123400.021.9816599
GAGTACT63350.021.1456612-13
GAGTACA70200.020.51711
AGAGTAC103950.020.17572410-11
GTGTAAG12800.019.7474961
AGTACTT67950.019.46948412-13
GTACTTT69750.019.2057814-15
Produced by FastQC (version 0.11.5)