##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938436_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2614933 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.746463102496318 31.0 31.0 34.0 27.0 34.0 2 30.935581141084686 31.0 31.0 34.0 27.0 34.0 3 30.98227029143768 31.0 31.0 34.0 27.0 34.0 4 34.64361037166153 37.0 35.0 37.0 32.0 37.0 5 34.615330488391095 37.0 35.0 37.0 30.0 37.0 6 34.57615740059114 37.0 35.0 37.0 30.0 37.0 7 34.47074322745554 37.0 35.0 37.0 30.0 37.0 8 34.417956788950235 37.0 35.0 37.0 30.0 37.0 9 35.847687875750545 38.0 35.0 39.0 30.0 39.0 10-11 35.65987522433653 38.0 35.0 39.0 30.0 39.0 12-13 35.44806769427744 38.0 35.0 39.0 30.0 39.0 14-15 36.54992020828067 39.0 36.0 41.0 30.0 41.0 16-17 36.46376733170601 39.0 36.0 41.0 29.5 41.0 18-19 36.427959913313266 39.0 36.0 41.0 29.0 41.0 20-21 36.34391569497191 39.0 36.0 41.0 29.0 41.0 22-23 36.163564228987894 39.0 35.0 40.5 27.0 41.0 24-25 36.00356777783599 39.0 35.0 40.5 27.0 41.0 26-27 35.759610475679494 38.5 34.5 40.0 27.0 41.0 28-29 35.49964339430494 38.0 34.0 40.0 27.0 41.0 30-31 35.212389954159434 38.0 34.0 40.0 25.5 41.0 32-33 34.903587587138944 38.0 34.0 40.0 25.0 41.0 34-35 34.56513359998134 38.0 33.0 40.0 24.0 41.0 36-37 34.322096780299916 38.0 33.0 40.0 24.0 41.0 38-39 34.04894255416869 38.0 33.0 40.0 23.5 41.0 40-41 33.74388961399776 38.0 33.0 40.0 21.5 41.0 42-43 33.30284638267979 37.5 31.5 40.0 18.5 41.0 44-45 32.804677404736566 37.0 31.0 40.0 15.5 41.0 46-47 32.30114442702738 37.0 30.5 39.5 12.0 41.0 48-49 31.79418421045587 36.5 30.0 39.0 9.0 40.5 50-51 31.076471748989363 35.5 29.5 38.5 8.0 40.0 52-53 31.076245548165097 36.0 30.0 39.0 7.0 40.0 54-55 31.00084304263245 36.0 29.0 39.0 2.0 40.0 56-57 30.52178507059263 35.0 28.0 39.0 2.0 40.0 58-59 30.01482255950726 35.0 28.0 39.0 2.0 40.0 60-61 29.41210424894252 34.5 26.5 38.0 2.0 40.0 62-63 28.634661193996173 34.0 26.0 38.0 2.0 40.0 64-65 27.927225286460494 34.0 24.0 37.5 2.0 40.0 66-67 27.18007115287466 33.0 22.5 37.0 2.0 39.0 68-69 26.26958491861933 32.0 19.0 36.0 2.0 39.0 70-71 25.39154177181595 31.5 15.5 35.5 2.0 39.0 72-73 24.442520898240986 31.0 7.5 35.0 2.0 38.0 74-75 23.475562853809258 30.0 2.0 34.5 2.0 37.0 76-77 22.4863365524088 29.0 2.0 34.0 2.0 36.5 78-79 21.46691291899257 28.5 2.0 34.0 2.0 36.0 80-81 20.47637434687619 26.5 2.0 33.5 2.0 35.0 82-83 19.460008153172566 26.0 2.0 33.0 2.0 35.0 84-85 18.46986653195321 24.5 2.0 32.0 2.0 35.0 86-87 17.468760958693778 21.0 2.0 32.0 2.0 34.0 88-89 16.370906826293446 15.0 2.0 31.0 2.0 34.0 90-91 15.231663105708636 4.5 2.0 31.0 2.0 34.0 92-93 14.106103483339727 2.0 2.0 30.0 2.0 34.0 94-95 12.9081173399089 2.0 2.0 29.0 2.0 34.0 96-97 11.58119997720783 2.0 2.0 28.0 2.0 33.5 98-99 10.016880164807283 2.0 2.0 23.5 2.0 33.0 100-101 7.139070484788712 2.0 2.0 2.0 2.0 28.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8755425582109773 1101 2 0.7911339079087334 1101 3 0.77851332799926 1101 4 0.6832166781919113 1101 5 0.6175793661196423 1101 6 0.7189263426350081 1101 7 0.6913719823390849 1101 8 0.7562392053755644 1101 9 0.8472546746180569 1101 10-11 0.9284053886641885 1101 12-13 0.8715734808385278 1101 14-15 1.0509767431291408 1101 16-17 1.0941405892202454 1101 18-19 1.0918049395425058 1101 20-21 1.2056889947775318 1101 22-23 1.310506321342956 1101 24-25 1.3115361437946902 1101 26-27 1.3000050512774166 1101 28-29 1.3561015598218518 1101 30-31 1.3813571854496445 1101 32-33 1.4326611383913033 1101 34-35 1.5584787872144616 1101 36-37 1.6306917086009136 1101 38-39 1.7212547193430794 1101 40-41 1.7478792419152214 1101 42-43 1.8264820502566579 1101 44-45 1.8002398722726483 1101 46-47 1.925799195465295 1101 48-49 1.9545888186187064 1101 50-51 2.08481877535975 1101 52-53 2.1314111219374468 1101 54-55 2.3831675632591605 1101 56-57 2.625594951247919 1101 58-59 2.6027770293194195 1101 60-61 2.5515414371643956 1101 62-63 2.5123209388968455 1101 64-65 2.5549809432665427 1101 66-67 2.8736346031244793 1101 68-69 2.902268956444967 1101 70-71 3.1601998349182914 1101 72-73 3.5313782026493143 1101 74-75 3.590349861053415 1101 76-77 3.776737201009219 1101 78-79 3.902760553607834 1101 80-81 4.1900737050744254 1101 82-83 4.239283490499762 1101 84-85 4.196538710892874 1101 86-87 4.170566671933262 1101 88-89 4.4088301977693725 1101 90-91 4.558921288057785 1101 92-93 4.4048165271566635 1101 94-95 4.271159505833772 1101 96-97 4.092576502777934 1101 98-99 3.705099472832229 1101 100-101 2.9713633415343805 1102 1 0.93276506063863 1102 2 0.7277409734483768 1102 3 0.6658240323387581 1102 4 0.5213169558927504 1102 5 0.6955392003055962 1102 6 0.7524305755732001 1102 7 0.7526585284902438 1102 8 0.8318483987871588 1102 9 0.7711622645568355 1102 10-11 1.0012602141211744 1102 12-13 0.9675594113677093 1102 14-15 1.1148456228531458 1102 16-17 1.0890420255833888 1102 18-19 1.1498598504052282 1102 20-21 1.1894946266642705 1102 22-23 1.005630328220363 1102 24-25 1.0943793416444763 1102 26-27 1.2046857746348962 1102 28-29 1.280360159392579 1102 30-31 1.0216018940290184 1102 32-33 1.2957951454075314 1102 34-35 1.4164777084052034 1102 36-37 1.5163506515132568 1102 38-39 1.7595893009309975 1102 40-41 1.7808262222738804 1102 42-43 1.7921930034097642 1102 44-45 1.9484874021497731 1102 46-47 2.0420309781645543 1102 48-49 2.0445789956372913 1102 50-51 2.0666763368409704 1102 52-53 1.9490416218320092 1102 54-55 1.7841917710251316 1102 56-57 2.206049036081197 1102 58-59 2.4377603613196683 1102 60-61 2.554199459254864 1102 62-63 2.543058905248838 1102 64-65 2.7507754049823667 1102 66-67 3.0440421775289366 1102 68-69 3.017384911915002 1102 70-71 2.809964275064541 1102 72-73 2.822136634647645 1102 74-75 2.8027356424530794 1102 76-77 3.1139260765819223 1102 78-79 3.639613557252119 1102 80-81 3.9623348879702753 1102 82-83 4.0706119594962225 1102 84-85 3.9895580481523147 1102 86-87 3.8813585324760105 1102 88-89 3.9870709718679045 1102 90-91 3.9300019283892524 1102 92-93 4.010406471226487 1102 94-95 3.622274407613876 1102 96-97 3.67765899004144 1102 98-99 3.499075988899678 1102 100-101 2.388946478264862 1103 1 0.7360041982292032 1103 2 0.5983838778663397 1103 3 0.5228447602218331 1103 4 0.4846021127937732 1103 5 0.4931901873727824 1103 6 0.4364798270476484 1103 7 0.5319758458069188 1103 8 0.47781543928741854 1103 9 0.6936965319249992 1103 10-11 0.7247114608747012 1103 12-13 0.6680493861892032 1103 14-15 0.9203362230587047 1103 16-17 0.8558603785424808 1103 18-19 0.977239052914193 1103 20-21 0.8480547590217711 1103 22-23 1.0439514167149255 1103 24-25 0.9843578098789649 1103 26-27 1.1769026475930033 1103 28-29 1.2320696331454855 1103 30-31 1.1582038481063108 1103 32-33 1.3542947866514012 1103 34-35 1.2121321048551934 1103 36-37 1.350477204297114 1103 38-39 1.4730443991546878 1103 40-41 1.5797905958549805 1103 42-43 1.6106583237039729 1103 44-45 1.597097210155269 1103 46-47 1.8609508615072272 1103 48-49 1.6983300684668698 1103 50-51 1.8113210659089134 1103 52-53 1.8982519584869664 1103 54-55 2.056781811136087 1103 56-57 2.215891877544891 1103 58-59 2.274140296055805 1103 60-61 2.310549106848544 1103 62-63 2.66101921591277 1103 64-65 2.789475242702398 1103 66-67 2.9386322534675138 1103 68-69 3.065466310369743 1103 70-71 3.0317572943708306 1103 72-73 3.2323702691783147 1103 74-75 3.2225358138498983 1103 76-77 3.3304528342230455 1103 78-79 3.689534720472885 1103 80-81 3.6723351790697656 1103 82-83 3.848584061288914 1103 84-85 4.007748007523144 1103 86-87 3.9616004265548206 1103 88-89 4.518232212514729 1103 90-91 4.5967410766961265 1103 92-93 4.434540746144043 1103 94-95 4.187543551483881 1103 96-97 3.91383632273201 1103 98-99 3.414883538868825 1103 100-101 2.5272454463971448 1104 1 0.6301214121544163 1104 2 0.5414719765414198 1104 3 0.5991907186987149 1104 4 0.4566594032698532 1104 5 0.4658982522220825 1104 6 0.5856240957141097 1104 7 0.5117466157018242 1104 8 0.5093469891700337 1104 9 0.7129448475389495 1104 10-11 0.7695291889765485 1104 12-13 0.8946614560093096 1104 14-15 0.8995035215634672 1104 16-17 0.941905485520401 1104 18-19 0.9062577204668614 1104 20-21 0.8223701445320302 1104 22-23 0.8737165811686083 1104 24-25 0.9819928116118959 1104 26-27 0.9964269802625836 1104 28-29 1.1289489037108495 1104 30-31 1.1362582062501048 1104 32-33 1.1406155183795121 1104 34-35 1.227583959999734 1104 36-37 1.3032424220138097 1104 38-39 1.3802485454007254 1104 40-41 1.3978262048163757 1104 42-43 1.652603772425202 1104 44-45 1.6889351596411188 1104 46-47 1.6811725925429215 1104 48-49 1.7159406597481244 1104 50-51 1.7456572073947854 1104 52-53 1.7484403179093704 1104 54-55 2.1277256482363818 1104 56-57 2.3884668150513946 1104 58-59 2.31629095070042 1104 60-61 2.5260780952784003 1104 62-63 2.55442198127383 1104 64-65 2.789269131458756 1104 66-67 2.9125988643513665 1104 68-69 3.288265774842639 1104 70-71 3.3476161362338672 1104 72-73 3.5877113342483575 1104 74-75 3.435040881757214 1104 76-77 3.580136688847322 1104 78-79 3.533148644237979 1104 80-81 3.6263421943963827 1104 82-83 3.6526283229994974 1104 84-85 3.862340523632941 1104 86-87 3.909533723765879 1104 88-89 3.9432050038690427 1104 90-91 3.7610072112759063 1104 92-93 3.9421582616163438 1104 94-95 3.891409902676614 1104 96-97 3.6766848917752775 1104 98-99 3.4062016685556475 1104 100-101 2.251909795142196 1105 1 0.608569862302808 1105 2 0.5883933630762463 1105 3 0.4042857119376535 1105 4 0.4236959811042098 1105 5 0.48113607262767744 1105 6 0.4435179387425521 1105 7 0.5509471547140237 1105 8 0.5290389809827047 1105 9 0.6184244785896951 1105 10-11 0.7859632299974919 1105 12-13 0.7370604007984412 1105 14-15 0.7183894211422199 1105 16-17 0.8320960082864772 1105 18-19 0.8160638352589231 1105 20-21 0.8434281815554954 1105 22-23 0.9482932007908857 1105 24-25 1.0818503121769183 1105 26-27 0.8975705509970808 1105 28-29 0.9437900836640551 1105 30-31 1.0427348651576125 1105 32-33 1.0849564648771022 1105 34-35 1.1269477774863859 1105 36-37 1.1526556759083846 1105 38-39 1.240790417804142 1105 40-41 1.3735844886601 1105 42-43 1.495321297940393 1105 44-45 1.4247651273403932 1105 46-47 1.6079264783978076 1105 48-49 1.601830023493246 1105 50-51 1.415724835038823 1105 52-53 1.642023927580098 1105 54-55 1.8683336883881942 1105 56-57 1.9825130371351491 1105 58-59 2.09446150992558 1105 60-61 2.2968749046190062 1105 62-63 2.2420690083006285 1105 64-65 2.409220201545242 1105 66-67 2.6486552648445816 1105 68-69 2.7972347692980115 1105 70-71 2.878941538460655 1105 72-73 2.8861335828877372 1105 74-75 3.0222749668729634 1105 76-77 3.048294364387008 1105 78-79 3.431095128634759 1105 80-81 3.555069918751059 1105 82-83 3.7327308391700775 1105 84-85 3.6281196333349506 1105 86-87 3.706287945864954 1105 88-89 3.654958823751972 1105 90-91 3.5342626466206184 1105 92-93 3.6977208683685507 1105 94-95 3.5378727628037154 1105 96-97 3.452845859954234 1105 98-99 2.908835439474613 1105 100-101 2.0230817934073473 1106 1 0.4798948500064917 1106 2 0.34171295125608125 1106 3 0.12457851877983472 1106 4 0.22263970864620575 1106 5 0.26774799249827197 1106 6 -0.03527310569705122 1106 7 0.26604168813453555 1106 8 0.012302628224944101 1106 9 0.3472023707712424 1106 10-11 0.3232893383570641 1106 12-13 0.48944555235838294 1106 14-15 0.6675409126082812 1106 16-17 0.264995692882799 1106 18-19 0.5478801078171358 1106 20-21 0.5861086367989898 1106 22-23 0.5629863189449296 1106 24-25 0.6229396364681747 1106 26-27 0.48722865909277857 1106 28-29 0.7052168907216867 1106 30-31 0.4568717054257405 1106 32-33 0.6326718132699796 1106 34-35 0.5487930935706373 1106 36-37 0.6260152553033507 1106 38-39 0.8126021643154502 1106 40-41 1.0324612607702974 1106 42-43 0.6707661574116415 1106 44-45 1.047527260194066 1106 46-47 1.2411244061029763 1106 48-49 1.09056918540632 1106 50-51 0.881805370281441 1106 52-53 1.1384239666610938 1106 54-55 1.069641941409241 1106 56-57 1.5507325470694866 1106 58-59 1.7071308504671805 1106 60-61 1.8328081262207796 1106 62-63 1.87289952955593 1106 64-65 1.7216873730916014 1106 66-67 2.0256259115097954 1106 68-69 2.261372945668544 1106 70-71 2.0191800995451707 1106 72-73 2.3113561065680663 1106 74-75 2.8371172692112268 1106 76-77 2.8858508251519446 1106 78-79 3.195741316191807 1106 80-81 3.1955779967426956 1106 82-83 3.2261417666506134 1106 84-85 3.0906887244429377 1106 86-87 2.8559374695026882 1106 88-89 2.852691959008123 1106 90-91 2.7960527065197596 1106 92-93 2.804553111525829 1106 94-95 2.7396192156605945 1106 96-97 2.4885719856831408 1106 98-99 2.2921997536824747 1106 100-101 1.5439489990243223 1107 1 0.1425632415656075 1107 2 0.1129315003528859 1107 3 0.14031283203970446 1107 4 0.191659073175785 1107 5 0.18514931090030728 1107 6 0.2102570188885835 1107 7 0.1329272711572429 1107 8 0.19647514092310558 1107 9 0.3170537986847606 1107 10-11 0.23514432266192387 1107 12-13 0.316967501294954 1107 14-15 0.28513849349459974 1107 16-17 0.3037897819812798 1107 18-19 0.45580864311373404 1107 20-21 0.43383903866801177 1107 22-23 0.4963306327431596 1107 24-25 0.4289418730716079 1107 26-27 0.43230994384980903 1107 28-29 0.5211020787674769 1107 30-31 0.38364333147801233 1107 32-33 0.6496139776688636 1107 34-35 0.6499174452035064 1107 36-37 0.6646281134277459 1107 38-39 0.7242659232910498 1107 40-41 0.8274554480533212 1107 42-43 0.8598829442504439 1107 44-45 0.735226980725237 1107 46-47 1.0367666273764016 1107 48-49 0.8153174475878409 1107 50-51 1.0129766180940152 1107 52-53 1.1452457137383405 1107 54-55 1.0371017867552617 1107 56-57 1.2891966625370692 1107 58-59 1.5028867408736133 1107 60-61 1.6871983666130035 1107 62-63 1.6337306121604733 1107 64-65 1.8848453547968695 1107 66-67 1.968252316116473 1107 68-69 1.9033119785027957 1107 70-71 1.9899444784906102 1107 72-73 2.1621247924143 1107 74-75 2.2852532286879814 1107 76-77 2.22238834875861 1107 78-79 2.612885446520327 1107 80-81 2.7033040330773765 1107 82-83 2.7169510908776253 1107 84-85 2.6953254922631373 1107 86-87 2.5944631501982336 1107 88-89 2.8395087382245556 1107 90-91 2.584702477129319 1107 92-93 2.5312763915506036 1107 94-95 2.360364352140845 1107 96-97 2.039250295906859 1107 98-99 1.8419171399693663 1107 100-101 1.1675821043797496 1108 1 0.3027000980924228 1108 2 0.24702403746568535 1108 3 0.2426108624782195 1108 4 0.1983968650967256 1108 5 0.23441863167516885 1108 6 0.27203961910637275 1108 7 0.18034264497627817 1108 8 0.31396878966296526 1108 9 0.17481968846301754 1108 10-11 0.25924620809365706 1108 12-13 0.1390200271938582 1108 14-15 0.3013553862493481 1108 16-17 0.3804049800784952 1108 18-19 0.4405766077690103 1108 20-21 0.4409183202325835 1108 22-23 0.46064205800912816 1108 24-25 0.43303431818345217 1108 26-27 0.4999587547502884 1108 28-29 0.5978015471808646 1108 30-31 0.5552924459816211 1108 32-33 0.7535016439957261 1108 34-35 0.6757403234379424 1108 36-37 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-0.95448259367258 2315 96-97 -1.0450152822630123 2315 98-99 -1.2297834481700303 2315 100-101 -0.712864455192876 2316 1 -1.324190348799732 2316 2 -1.0465955859555933 2316 3 -1.0139307565119715 2316 4 -0.900924318453832 2316 5 -0.840401735054435 2316 6 -0.7175663375244881 2316 7 -1.0383352765148928 2316 8 -0.7456271221920332 2316 9 -1.045326833638157 2316 10-11 -1.2249152284541367 2316 12-13 -1.1199531238037892 2316 14-15 -1.3650883666903368 2316 16-17 -1.2851168794425334 2316 18-19 -1.2664145104943074 2316 20-21 -1.418261888331827 2316 22-23 -1.4041188709890662 2316 24-25 -1.4140379078969332 2316 26-27 -1.4667375278695047 2316 28-29 -1.4666216633917344 2316 30-31 -1.2663712997465524 2316 32-33 -1.5386165992535368 2316 34-35 -1.3135244335270144 2316 36-37 -1.706879634262883 2316 38-39 -1.862813105134677 2316 40-41 -1.6284277266988738 2316 42-43 -1.3081825792502073 2316 44-45 -1.630436786622031 2316 46-47 -0.9689541560240684 2316 48-49 -0.9042362695814496 2316 50-51 -0.9192930048928574 2316 52-53 -1.064614055118792 2316 54-55 -1.085151365943414 2316 56-57 -1.0383069152545588 2316 58-59 -0.7344074630785435 2316 60-61 -1.0368917922119856 2316 62-63 -0.8558572140510599 2316 64-65 -0.7023068297516453 2316 66-67 -0.6087763819128327 2316 68-69 -0.4773435867222382 2316 70-71 -0.5731224497091993 2316 72-73 -0.5045535589108674 2316 74-75 -0.40120851955030545 2316 76-77 -0.4054407991808553 2316 78-79 -0.5096836999864927 2316 80-81 -0.4778298919061008 2316 82-83 -0.47044441398344716 2316 84-85 -0.20718114640099472 2316 86-87 -0.22114595326840103 2316 88-89 -0.39535670785252464 2316 90-91 -0.3207685085935541 2316 92-93 -0.14180172343964337 2316 94-95 -0.2868324982848591 2316 96-97 -0.3547994441602924 2316 98-99 -0.6147114397908737 2316 100-101 -0.29107490669476555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12569.0 3 5283.0 4 5533.0 5 6357.0 6 7463.0 7 9310.0 8 11638.0 9 14675.0 10 18270.0 11 22080.0 12 26043.0 13 30441.0 14 33804.0 15 37338.0 16 42090.0 17 47221.0 18 48489.0 19 50154.0 20 54985.0 21 60537.0 22 66040.0 23 73429.0 24 80012.0 25 87103.0 26 95330.0 27 103372.0 28 111644.0 29 121663.0 30 132067.0 31 144789.0 32 158092.0 33 173421.0 34 190330.0 35 197720.0 36 185571.0 37 121621.0 38 28093.0 39 356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4194038308269 18.438868166175514 14.156138521570478 23.98558948142711 2 15.630091245057226 19.473186316569432 39.99871469671624 24.8980077416571 3 19.61707021106318 23.25593227860401 28.957109476496594 28.169888033836216 4 13.054812706355856 15.791277866409569 36.20022113313516 34.953688294099415 5 14.202327557870383 36.93510534776568 33.90525455009515 14.957312544268792 6 33.677947223568836 34.983717567617724 16.925809424947573 14.41252578386587 7 29.687694865534652 31.683232707799824 20.040994926514415 18.58807750015111 8 26.750303567288647 35.05285934753883 19.198306909398074 18.99853017577445 9 26.51608761751157 16.600624790072523 19.63069596839783 37.25259162401808 10-11 25.421587442345604 26.171579934578176 26.939131994967784 21.467700628108428 12-13 26.13738211900238 23.290737488538486 27.302430625552994 23.269449766906142 14-15 22.88558945751154 24.84213751589144 25.438337952618017 26.833935073979003 16-17 22.821750318673555 28.05356201965879 26.159020254537214 22.965667407130447 18-19 22.789149605100434 26.864646246723193 28.026565588290758 22.31963855988561 20-21 23.311781080165545 26.023221958241177 28.283272466373337 22.381724495219938 22-23 23.044984750688823 25.978265547459355 28.032432401415043 22.944317300436772 24-25 22.905342435566656 26.082592667407333 28.16751368854692 22.844551208479093 26-27 22.54199573792586 26.414587992167515 28.043575605143943 22.999840664762676 28-29 22.631263150988893 26.723153716399406 27.987446585538976 22.658136547072722 30-31 22.720821534838493 26.307921014034246 27.938657263065437 23.03260018806182 32-33 22.55453009945201 26.338055727322057 27.919070467916175 23.188343705309762 34-35 23.186456524055142 26.40353127111721 27.77289724935203 22.637114955475617 36-37 23.112723411108576 26.05977419894104 27.769138634719564 23.058363755230825 38-39 22.757953594256396 26.442962594933572 27.7168075048417 23.08227630596833 40-41 23.12824310054085 26.218958314289548 27.791060086308022 22.86173849886158 42-43 22.874599958501822 26.199483854435098 27.66339430251026 23.26252188455283 44-45 22.7825557829501 26.452942141623158 27.770884973589126 22.993617101837614 46-47 23.04268841118389 26.628931103879005 27.233806790527083 23.094573694410027 48-49 22.97572220641288 26.52509783942401 27.32764503030804 23.171534923855074 50-51 23.075596238976342 26.76372324437532 27.182381804444596 22.978298712203745 52-53 23.18969394529995 26.609154563283838 27.149408573692696 23.051742917723512 54-55 22.841784811433683 26.67701827722174 27.331751270685693 23.149445640658882 56-57 22.92732039492178 26.798785326778074 27.22451375781879 23.049380520481357 58-59 22.989904274463306 26.477353408141557 27.20990875278389 23.322833564611248 60-61 22.783120577466008 26.633225406484947 27.390988911300347 23.192665104748706 62-63 22.845314400955544 26.619315324218757 27.475437335708676 23.059932939117026 64-65 22.622220785923524 26.585034757684827 27.37400255588928 23.41874190050236 66-67 22.6481525815366 26.617595430386125 27.255914133112647 23.47833785496463 68-69 22.885454552408053 26.33837775406632 27.41715332514906 23.359014368376567 70-71 22.784883231723644 26.332049506995386 27.196777302664287 23.68628995861668 72-73 22.822524031524814 26.38693647324477 27.117496357527056 23.673043137703356 74-75 22.649910656369713 26.670909469200925 27.022432288543207 23.656747585886155 76-77 22.842471863317016 26.307504587861196 27.08638449766625 23.763639051155536 78-79 22.866988310300055 26.33462029032067 27.07751764546152 23.72087375391776 80-81 22.915530438919838 26.408565442511144 26.881401918271152 23.79450220029786 82-83 22.89334289758064 26.344044793110683 26.90956183138635 23.853050477922327 84-85 22.936582787576164 26.216977293136235 26.879955605121168 23.966484314166433 86-87 22.92013919629228 26.24114749938668 26.760695614330128 24.07801768999091 88-89 22.955171509023913 26.21581657358315 26.67160196718452 24.157409950208415 90-91 22.907343562147293 26.120273929530047 26.718992720280276 24.25338978804238 92-93 22.99734496419029 25.811254567525953 26.76287187520796 24.428528593075796 94-95 22.835018077813487 25.725746994760733 26.811563298165826 24.627671629259954 96-97 22.948196778497206 25.52259917259275 26.702377418881042 24.826826630029007 98-99 22.851068207799777 25.52266817307188 26.41452100176967 25.211742617358667 100-101 23.751864949066047 24.67045466946105 26.48996340076346 25.087716980709445 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 256.0 1 186.0 2 156.5 3 218.0 4 208.5 5 207.5 6 342.0 7 696.0 8 1180.5 9 1426.0 10 1333.5 11 1221.5 12 1273.0 13 1383.0 14 1559.0 15 1723.0 16 1837.0 17 2060.0 18 2360.0 19 2632.0 20 2942.0 21 3512.5 22 4278.0 23 5315.0 24 6693.5 25 8391.5 26 10872.0 27 14070.5 28 17351.0 29 20834.0 30 24889.0 31 30122.0 32 36107.5 33 40277.0 34 43705.5 35 48221.0 36 53694.5 37 58780.0 38 62877.0 39 66915.0 40 71359.5 41 76497.0 42 80455.5 43 84693.0 44 89342.5 45 93094.0 46 96500.5 47 99850.0 48 102379.5 49 102749.0 50 101714.5 51 99880.0 52 97396.5 53 95515.0 54 93941.5 55 89641.5 56 83319.0 57 76957.5 58 70933.5 59 63576.0 60 53832.5 61 44989.0 62 37360.5 63 29565.5 64 23112.5 65 18235.5 66 14049.5 67 10598.0 68 8026.5 69 6084.5 70 4564.0 71 3412.0 72 2591.0 73 2009.0 74 1479.0 75 1073.0 76 774.5 77 516.0 78 340.0 79 212.5 80 117.0 81 66.0 82 45.5 83 31.0 84 21.0 85 15.0 86 13.0 87 7.0 88 2.5 89 3.0 90 3.5 91 3.0 92 1.5 93 1.5 94 1.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3888818566288314 2 0.02921680976147381 3 0.03552672286440991 4 0.042945650997559016 5 0.06256374446305125 6 0.052773818679101914 7 0.03636804461146806 8 0.03881552605745539 9 0.03575617424997122 10-11 0.03722848730732298 12-13 0.02868142319516408 14-15 0.027209110137812326 16-17 0.022199421553057 18-19 0.027687133857731725 20-21 0.034360344987806574 22-23 0.033671990831122635 24-25 0.04088058852750721 26-27 0.03623419796989062 28-29 0.03149220266829016 30-31 0.03437946593660335 32-33 0.028968237427115722 34-35 0.030727364716419122 36-37 0.03296451572564192 38-39 0.015583573269372484 40-41 0.013155212772181926 42-43 0.013786204082475535 44-45 0.011090150302130111 46-47 0.019560730619101904 48-49 0.017017644429130688 50-51 0.01042091709424295 52-53 0.009063329729671851 54-55 0.0077631052114910774 56-57 0.009101571627265402 58-59 0.004340455376868164 60-61 0.00761013762111687 62-63 0.01124311789250432 64-65 0.00717035579879102 66-67 0.0036329802713874508 68-69 0.006233429307748993 70-71 0.013499389850523897 72-73 0.011262238841301096 74-75 0.011816746356407603 76-77 0.005831889383016696 78-79 0.009101571627265402 80-81 0.010975424609349456 82-83 0.012791914745043181 84-85 0.008298491777800808 86-87 0.002466602394784111 88-89 0.01663522545319517 90-91 0.026157457953989643 92-93 0.010669489428601039 94-95 0.020669745649314914 96-97 0.019159190694369607 98-99 0.012237407229936676 100-101 0.12491715848933797 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2614933.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.64730622288511 #Duplication Level Percentage of deduplicated Percentage of total 1 78.12840399142792 46.601488375819805 2 12.492639589970265 14.903045983101887 3 4.110703782296419 7.355772218826198 4 1.7944160137485017 4.281283258532229 5 0.9953047229845887 2.96836227984728 6 0.5655384825991195 2.0239708231449285 7 0.3694906678057075 1.5427386106373748 8 0.26350063637053167 1.2573682518014562 9 0.1981664172432867 1.0638083675162109 >10 0.9919958292630022 10.43406562631522 >50 0.05775351654089883 2.370942763655049 >100 0.029118028416599547 3.2132046384836856 >500 0.0018713330144597897 0.7798772069600093 >1k 0.0010324595941749116 0.9892993707605938 >5k 6.452872463593198E-5 0.21477222459806694 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5580 0.21338978857202076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4435 0.1696028158274036 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2758 0.10547115356301673 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 3.824189759355211E-5 9 0.0 0.0 0.0 0.0 7.648379518710423E-5 10-11 0.0 0.0 0.0 3.824189759355211E-5 7.648379518710423E-5 12-13 0.0 0.0 0.0 3.824189759355211E-5 1.1472569278065632E-4 14-15 0.0 0.0 0.0 3.824189759355211E-5 2.485723343580887E-4 16-17 0.0 0.0 0.0 5.736284639032817E-5 3.059351807484169E-4 18-19 0.0 0.0 0.0 7.648379518710423E-5 3.059351807484169E-4 20-21 0.0 0.0 0.0 1.338466415774324E-4 3.2505612954519293E-4 22-23 0.0 0.0 0.0 1.9120948796776056E-4 3.8241897593552113E-4 24-25 0.0 0.0 0.0 3.8241897593552113E-4 4.206608735290732E-4 26-27 0.0 0.0 0.0 8.222007982613703E-4 4.206608735290732E-4 28-29 0.0 0.0 0.0 0.0027151747291421996 4.206608735290732E-4 30-31 0.0 0.0 0.0 0.009732562937559013 4.206608735290732E-4 32-33 0.0 0.0 0.0 0.02611921605639609 4.589027711226253E-4 34-35 0.0 0.0 0.0 0.052181069266401855 4.589027711226253E-4 36-37 0.0 0.0 0.0 0.09856849104738057 4.589027711226253E-4 38-39 0.0 0.0 0.0 0.1696792996225907 4.780237199194014E-4 40-41 0.0 0.0 0.0 0.24692793276156597 4.971446687161774E-4 42-43 0.0 0.0 0.0 0.3267005311417157 5.353865663097296E-4 44-45 0.0 0.0 0.0 0.4266074886048706 5.353865663097296E-4 46-47 0.0 0.0 0.0 0.5425760430573173 5.545075151065056E-4 48-49 0.0 0.0 0.0 0.6692714497847554 6.309913102936098E-4 50-51 0.0 0.0 0.0 0.8032519380037653 6.501122590903859E-4 52-53 0.0 0.0 0.0 0.934765823827991 6.501122590903859E-4 54-55 0.0 0.0 0.0 1.0822074599999312 6.501122590903859E-4 56-57 0.0 0.0 0.0 1.2553094094571446 6.501122590903859E-4 58-59 0.0 0.0 0.0 1.4425991029215663 6.501122590903859E-4 60-61 0.0 0.0 0.0 1.6459312724264827 6.692332078871619E-4 62-63 0.0 0.0 0.0 1.8483456363891542 7.648379518710423E-4 64-65 0.0 0.0 0.0 2.0568404620692005 7.648379518710423E-4 66-67 0.0 0.0 0.0 2.3065026905087054 7.648379518710423E-4 68-69 0.0 0.0 0.0 2.580697096254474 8.986845934484746E-4 70-71 0.0 0.0 0.0 2.8671097882813825 9.178055422452506E-4 72-73 0.0 0.0 0.0 3.15975590961604 0.0010134102862291309 74-75 0.0 0.0 0.0 3.4550598428334496 0.001051652183822683 76-77 0.0 0.0 0.0 3.7735766078901447 0.0010707731326194592 78-79 0.0 0.0 0.0 4.131386922724215 0.0011090150302130112 80-81 0.0 0.0 0.0 4.514723704201981 0.0011854988254001156 82-83 0.0 0.0 0.0 4.901865554490306 0.0013002245181807717 84-85 0.0 0.0 0.0 5.283921232398688 0.0013002245181807717 86-87 0.0 0.0 0.0 5.677487721482731 0.0013193454669775478 88-89 0.0 0.0 0.0 6.094783308023571 0.0013384664157743238 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4230 0.0 41.603718 1 GTATCAA 7270 0.0 40.016727 1 ATCAACG 8890 0.0 32.07661 3 TCAACGC 9005 0.0 31.66697 4 TATCAAC 9210 0.0 31.374943 2 CAACGCA 9285 0.0 30.60964 5 AACGCAG 9695 0.0 29.404766 6 ACGCAGA 11035 0.0 25.615377 7 GTACATG 9045 0.0 25.361948 1 CGCAGAG 11270 0.0 24.996943 8 TACATGG 8965 0.0 24.969486 2 ACATGGG 9415 0.0 23.170282 3 GGACCGA 955 0.0 22.88594 6 GCAGAGT 12340 0.0 21.981659 9 GAGTACT 6335 0.0 21.14566 12-13 GAGTACA 7020 0.0 20.5171 1 AGAGTAC 10395 0.0 20.175724 10-11 GTGTAAG 1280 0.0 19.747496 1 AGTACTT 6795 0.0 19.469484 12-13 GTACTTT 6975 0.0 19.20578 14-15 >>END_MODULE