FastQCFastQC Report
Thu 26 May 2016
SRR938436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614933
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55170.21098054902362698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48390.18505254245519867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31920.12206813711861833No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47300.042.491571
GTATCAA79150.038.2995151
ATCAACG102050.029.2771153
TCAACGC102550.029.0874944
TATCAAC103450.029.0673472
CAACGCA104800.028.5536485
AACGCAG108450.027.5921156
ACGCAGA122700.024.2328117
CGCAGAG125400.023.6731748
GCAGAGT137100.021.2367849
AGAGTAC117750.020.97529810-11
TACATGG88650.020.7914682
GTACATG92350.020.6315081
TAAGGTG13450.020.482745
AGTACTT74550.019.87812-13
GAGTACT69250.019.444612-13
ACATGGG92400.019.0204333
GTAAGGT12100.018.8424854
CAGAGTA131500.018.4208710-11
GTACTTT73550.018.2755114-15
Produced by FastQC (version 0.11.5)