##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938436_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2614933 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.767581043185427 31.0 31.0 34.0 30.0 34.0 2 32.01999745308962 31.0 31.0 34.0 30.0 34.0 3 32.08254666563158 33.0 31.0 34.0 30.0 34.0 4 35.74342363647558 37.0 35.0 37.0 33.0 37.0 5 35.63217107283437 37.0 35.0 37.0 33.0 37.0 6 35.66401433612257 37.0 35.0 37.0 33.0 37.0 7 35.576778066589085 37.0 35.0 37.0 33.0 37.0 8 35.54675320553146 37.0 35.0 37.0 33.0 37.0 9 37.080494987825695 39.0 37.0 39.0 33.0 39.0 10-11 37.00457143643833 39.0 37.0 39.0 33.0 39.0 12-13 36.88776787015193 39.0 37.0 39.0 33.0 39.0 14-15 37.897437333958464 40.0 37.0 41.0 33.0 41.0 16-17 37.98461815274044 40.0 37.0 41.0 33.0 41.0 18-19 38.11251378142384 40.0 37.0 41.0 33.0 41.0 20-21 38.056246182980594 40.0 37.0 41.0 33.0 41.0 22-23 37.92995403706328 40.0 37.0 41.0 32.5 41.0 24-25 37.81663794062793 40.0 37.0 41.0 32.0 41.0 26-27 37.59835586609676 39.5 37.0 41.0 32.0 41.0 28-29 37.459196086477164 39.0 36.5 41.0 31.5 41.0 30-31 37.204849990420406 39.0 36.0 41.0 31.0 41.0 32-33 37.116698018649046 39.0 36.0 41.0 31.0 41.0 34-35 36.99349581805728 39.0 36.0 41.0 30.5 41.0 36-37 36.87237244701872 39.0 36.0 41.0 30.0 41.0 38-39 36.659643287227624 39.0 35.0 40.0 30.0 41.0 40-41 36.44168340068369 39.0 35.0 40.0 30.0 41.0 42-43 36.187112442269076 38.0 35.0 40.0 30.0 41.0 44-45 35.90832231648 38.0 35.0 40.0 29.0 41.0 46-47 35.51771517664123 38.0 34.5 40.0 27.5 41.0 48-49 35.12027765147329 38.0 34.0 40.0 26.5 41.0 50-51 34.730543000528115 38.0 33.0 40.0 26.0 41.0 52-53 34.11490581211832 37.5 33.0 40.0 24.0 41.0 54-55 33.67071756714226 37.0 32.0 40.0 23.0 41.0 56-57 33.26834645476576 37.0 32.0 39.0 22.0 40.5 58-59 32.71855282716613 36.0 31.0 39.0 20.5 40.0 60-61 32.20037109937425 35.5 31.0 39.0 18.5 40.0 62-63 31.664114529894263 35.0 30.0 38.5 15.5 40.0 64-65 30.981445604916075 34.5 29.0 38.0 10.5 40.0 66-67 30.315470033075417 34.0 28.0 37.5 7.5 40.0 68-69 29.56756846160112 34.0 27.5 37.0 6.0 39.0 70-71 28.769412447661182 33.0 26.0 36.0 2.0 39.0 72-73 28.167623988836425 33.0 26.0 36.0 2.0 38.5 74-75 27.4328380497703 32.0 25.0 35.0 2.0 38.0 76-77 26.304419463137297 30.5 24.0 34.0 2.0 36.0 78-79 26.781994414388436 32.0 25.0 35.0 2.0 36.0 80-81 26.399192828267495 32.0 24.0 35.0 2.0 36.0 82-83 25.69432754108805 31.0 21.0 34.0 2.0 36.0 84-85 24.901292882073843 31.0 19.0 34.0 2.0 35.0 86-87 23.967278511533564 30.5 13.5 34.0 2.0 35.0 88-89 22.9693470157744 29.5 4.5 34.0 2.0 35.0 90-91 22.092551128461036 29.0 2.0 34.0 2.0 35.0 92-93 20.9792553002314 28.5 2.0 33.5 2.0 35.0 94-95 19.771437929767224 26.5 2.0 33.0 2.0 34.0 96-97 18.363990396694675 24.5 2.0 32.0 2.0 34.0 98-99 16.601620003265857 11.0 2.0 32.0 2.0 34.0 100-101 12.446782575308813 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3156681124289271 1101 2 0.3412812811096231 1101 3 0.33818253583496727 1101 4 0.22994303135810412 1101 5 0.26849184447088703 1101 6 0.2579698718985952 1101 7 0.3000345746223019 1101 8 0.24535653084134879 1101 9 0.37294337853719384 1101 10-11 0.46126173105029267 1101 12-13 0.3231950328718938 1101 14-15 0.5690519509547656 1101 16-17 0.5423867741400912 1101 18-19 0.48903416147257417 1101 20-21 0.5148534630631119 1101 22-23 0.5860990122129834 1101 24-25 0.5254573483375395 1101 26-27 0.6109192794943965 1101 28-29 0.6107595996227388 1101 30-31 0.6831083866121048 1101 32-33 0.5216313173861877 1101 34-35 0.4598358870213275 1101 36-37 0.5040052662467858 1101 38-39 0.6220846869750432 1101 40-41 0.7447870188799399 1101 42-43 0.767027083391433 1101 44-45 0.798010146457429 1101 46-47 0.9503714834738233 1101 48-49 0.8495214094630796 1101 50-51 1.026869322457813 1101 52-53 1.0731756699399213 1101 54-55 1.2299336428519112 1101 56-57 1.282890389581297 1101 58-59 1.326463408816572 1101 60-61 1.4337737385049536 1101 62-63 1.6073396575465857 1101 64-65 1.5789312800892077 1101 66-67 1.7896519071325656 1101 68-69 1.9071637468713583 1101 70-71 2.0942714993412537 1101 72-73 2.1646002603701824 1101 74-75 2.0601738878311586 1101 76-77 2.0257103101675575 1101 78-79 2.006318624156723 1101 80-81 2.276391223519756 1101 82-83 2.4669006913730023 1101 84-85 2.6816103288485813 1101 86-87 2.6490508672652204 1101 88-89 2.3623754759670845 1101 90-91 2.6492444807317206 1101 92-93 2.8817121826571857 1101 94-95 2.7329311587177862 1101 96-97 2.5953873784477004 1101 98-99 2.507671440352585 1101 100-101 2.5309422455979185 1102 1 0.28349883985177016 1102 2 0.2309267613813617 1102 3 0.27291821103934666 1102 4 0.2646913323326672 1102 5 0.1931883797876779 1102 6 0.24522081866610534 1102 7 0.2667170840965838 1102 8 0.2657686333361582 1102 9 0.34581218488630583 1102 10-11 0.32355027418844884 1102 12-13 0.32320511365698934 1102 14-15 0.36648799415737443 1102 16-17 0.3443109150754182 1102 18-19 0.32642614007619386 1102 20-21 0.3133174158281875 1102 22-23 0.46318449528750705 1102 24-25 0.3801958792135025 1102 26-27 0.47020639281365817 1102 28-29 0.37156322659005525 1102 30-31 0.3889031524040689 1102 32-33 0.3978749722756376 1102 34-35 0.4904685835354883 1102 36-37 0.42040515016902447 1102 38-39 0.45093989318824157 1102 40-41 0.5615018199182913 1102 42-43 0.5916850473108184 1102 44-45 0.6341340945027198 1102 46-47 0.6728555674014771 1102 48-49 0.8096733880402525 1102 50-51 0.8829209995368856 1102 52-53 1.003709971381177 1102 54-55 0.9481092653321213 1102 56-57 1.0821738605972229 1102 58-59 1.1733597499212465 1102 60-61 1.1523768854285947 1102 62-63 1.2548159247343271 1102 64-65 1.3289917647997953 1102 66-67 1.3628993874320656 1102 68-69 1.5689449786309204 1102 70-71 1.705905783000663 1102 72-73 1.624820821337778 1102 74-75 1.632751904844877 1102 76-77 1.7808667143746497 1102 78-79 1.8348654575011842 1102 80-81 1.8740718827568337 1102 82-83 1.88416173875666 1102 84-85 2.2121597349416042 1102 86-87 2.083954280123276 1102 88-89 1.616361021773841 1102 90-91 1.8326071860574835 1102 92-93 2.2618871784761367 1102 94-95 1.9588668373595475 1102 96-97 2.0295529108883272 1102 98-99 1.8692330101522714 1102 100-101 1.6153898086590122 1103 1 0.21877382810382429 1103 2 0.23279763540597997 1103 3 0.2695237030950466 1103 4 0.1990944616201915 1103 5 0.12936593640093008 1103 6 0.20643341118216085 1103 7 0.2677867947468897 1103 8 0.23660206396922945 1103 9 0.31044103783595745 1103 10-11 0.3491971788252144 1103 12-13 0.3267906756341503 1103 14-15 0.4713051628853506 1103 16-17 0.37486388574570384 1103 18-19 0.3620172911635464 1103 20-21 0.3167931757178124 1103 22-23 0.4358501732822049 1103 24-25 0.416840506556575 1103 26-27 0.42531893155628353 1103 28-29 0.37103588866929726 1103 30-31 0.3624670002786772 1103 32-33 0.3509898007876515 1103 34-35 0.39125220096806146 1103 36-37 0.44153337501555256 1103 38-39 0.4912355798935337 1103 40-41 0.44960293636480486 1103 42-43 0.47692627122102493 1103 44-45 0.5939566869579878 1103 46-47 0.6580489998131327 1103 48-49 0.7446942240460004 1103 50-51 0.877895534729376 1103 52-53 0.8687021868690081 1103 54-55 0.9265616652874726 1103 56-57 1.1669357140244756 1103 58-59 1.2549319046876448 1103 60-61 1.3354926192280345 1103 62-63 1.4042100219404396 1103 64-65 1.6133078361466744 1103 66-67 1.5608663134118643 1103 68-69 1.361351216605076 1103 70-71 1.5394353419145617 1103 72-73 1.5506901771665973 1103 74-75 1.396194943232583 1103 76-77 1.5486149062789458 1103 78-79 1.9066056470485364 1103 80-81 2.059046814612362 1103 82-83 2.198490567298741 1103 84-85 2.3769865035549422 1103 86-87 2.3086411802304063 1103 88-89 2.1370674722506173 1103 90-91 2.4472623179335464 1103 92-93 2.6468574559387363 1103 94-95 2.229494195145449 1103 96-97 2.1782711614507697 1103 98-99 2.21878119815392 1103 100-101 1.939550522447755 1104 1 0.1557054473037951 1104 2 0.3060312537492109 1104 3 0.30538902101560694 1104 4 0.1696854350642809 1104 5 0.14799395675675697 1104 6 0.14954127601603773 1104 7 0.07208854462531633 1104 8 0.13057065448669647 1104 9 0.2622565746101415 1104 10-11 0.2635286850713996 1104 12-13 0.3043578205966 1104 14-15 0.4010256045186793 1104 16-17 0.3521075296053908 1104 18-19 0.41467192401167097 1104 20-21 0.3382700125711082 1104 22-23 0.34219547238417647 1104 24-25 0.3541690332429255 1104 26-27 0.3982354463931941 1104 28-29 0.4230088153689806 1104 30-31 0.4708234230505539 1104 32-33 0.5290299303148345 1104 34-35 0.4491577667109823 1104 36-37 0.5433989533665624 1104 38-39 0.6177629117382253 1104 40-41 0.6069257172853 1104 42-43 0.6083776399068128 1104 44-45 0.5517039574551532 1104 46-47 0.5659096541273101 1104 48-49 0.605890070617761 1104 50-51 0.7418153390474203 1104 52-53 0.7954435967755344 1104 54-55 0.8149928644942861 1104 56-57 0.8686516278494523 1104 58-59 0.9331577757528464 1104 60-61 0.9892235420556688 1104 62-63 0.948429861460351 1104 64-65 1.1533842512112713 1104 66-67 0.9986110610832668 1104 68-69 1.2349246770815832 1104 70-71 1.3523199804254702 1104 72-73 1.406770662551029 1104 74-75 1.584263908718608 1104 76-77 1.5177635536776855 1104 78-79 1.6920497270009882 1104 80-81 1.8877736521823643 1104 82-83 1.9066262579049393 1104 84-85 2.0993477930267126 1104 86-87 2.1059678902407875 1104 88-89 1.9594149706772477 1104 90-91 2.283906407422041 1104 92-93 2.411550539548532 1104 94-95 2.160175935997831 1104 96-97 2.4064476246633113 1104 98-99 2.473989920492045 1104 100-101 2.072333164561533 1105 1 0.12602444939915003 1105 2 0.13146859464506377 1105 3 0.15677462372054407 1105 4 0.1552214019131597 1105 5 0.07455354454692298 1105 6 0.08379532254456734 1105 7 0.09251771989493562 1105 8 0.1835606101492857 1105 9 0.14808124327128525 1105 10-11 0.19925113975610742 1105 12-13 0.2562308496842718 1105 14-15 0.2529904836005912 1105 16-17 0.2749748157274823 1105 18-19 0.28330153316398565 1105 20-21 0.21377262314543088 1105 22-23 0.20073818156743073 1105 24-25 0.1721123850356321 1105 26-27 0.20119124560519452 1105 28-29 0.2895773327832387 1105 30-31 0.3362235983231585 1105 32-33 0.4302696924241971 1105 34-35 0.3637630224204358 1105 36-37 0.297640844029317 1105 38-39 0.27595984272261376 1105 40-41 0.4480918376615719 1105 42-43 0.3827805617411073 1105 44-45 0.40323150628186255 1105 46-47 0.4443399300646078 1105 48-49 0.4574901886011631 1105 50-51 0.5161256873240418 1105 52-53 0.4192576443766498 1105 54-55 0.4700280360618194 1105 56-57 0.6911796052810786 1105 58-59 0.8865707180895228 1105 60-61 0.8947716988203638 1105 62-63 0.9031394720427635 1105 64-65 1.0551790290411134 1105 66-67 1.1605871213484384 1105 68-69 1.1481702719635187 1105 70-71 1.120115491182517 1105 72-73 1.4338276789244198 1105 74-75 1.4454720979244904 1105 76-77 1.4139858289612235 1105 78-79 1.4077805920466169 1105 80-81 1.462411774696978 1105 82-83 1.6882282103344757 1105 84-85 1.7969899977160004 1105 86-87 1.9053812079792039 1105 88-89 1.7649383560347935 1105 90-91 2.059303377734235 1105 92-93 2.113826742440825 1105 94-95 2.1437072064943585 1105 96-97 2.307121091975052 1105 98-99 1.869372649373254 1105 100-101 2.16466177472811 1106 1 0.050821925501708165 1106 2 0.06530355092528595 1106 3 0.0703651233643896 1106 4 0.10239107773296041 1106 5 0.08494174075021732 1106 6 0.006521422386491338 1106 7 0.10332470069597832 1106 8 0.03955465781306344 1106 9 0.005757527370079174 1106 10-11 0.14923915658766163 1106 12-13 0.08734750562036453 1106 14-15 0.10199588061924203 1106 16-17 0.04089259699583181 1106 18-19 0.08785188042534742 1106 20-21 0.09844235115673428 1106 22-23 0.22146259123980627 1106 24-25 0.14097911132587626 1106 26-27 -0.09985329420356237 1106 28-29 0.07919193404435276 1106 30-31 0.26309412081435823 1106 32-33 0.1600809763100557 1106 34-35 0.0682472326127268 1106 36-37 0.16471556472129123 1106 38-39 0.18915932364682675 1106 40-41 0.17100403151666654 1106 42-43 0.30030838816136196 1106 44-45 0.2777856864870003 1106 46-47 -0.16168752015931176 1106 48-49 0.22549354221819584 1106 50-51 0.4392290273295316 1106 52-53 0.25703623216650584 1106 54-55 0.2027227959516935 1106 56-57 0.40190320494137666 1106 58-59 0.5374592679424239 1106 60-61 0.6981949184430363 1106 62-63 0.5233352444477397 1106 64-65 0.5952405983100917 1106 66-67 0.6522481722096813 1106 68-69 0.6407073049644154 1106 70-71 0.506197938721705 1106 72-73 0.8530387856096944 1106 74-75 0.9053863117994077 1106 76-77 1.0000913660201114 1106 78-79 1.2362571931357103 1106 80-81 1.188534372113459 1106 82-83 1.390080716319794 1106 84-85 1.4220598299432936 1106 86-87 1.5224406887531075 1106 88-89 1.7177215408519935 1106 90-91 1.6475320650219736 1106 92-93 1.5757415609020846 1106 94-95 1.564607438892672 1106 96-97 1.6982627747793337 1106 98-99 1.875414128672432 1106 100-101 1.5165544055388 1107 1 -0.03081223419156487 1107 2 -0.00864375030564446 1107 3 0.011398960786344503 1107 4 0.10349152286821806 1107 5 0.017069141860737602 1107 6 0.041195438502185766 1107 7 -0.027909444925235505 1107 8 0.10449797782592185 1107 9 -0.03571105117675444 1107 10-11 0.03142596414541998 1107 12-13 0.13573164492515133 1107 14-15 0.16648399106779266 1107 16-17 0.05300522245163819 1107 18-19 0.01473824167518245 1107 20-21 0.10088497156473153 1107 22-23 0.10476023806221235 1107 24-25 0.17640910046800684 1107 26-27 0.15106338805430397 1107 28-29 0.24083024072874082 1107 30-31 0.16367680504763626 1107 32-33 0.15335877301047418 1107 34-35 0.21041200461616683 1107 36-37 0.3196010187501841 1107 38-39 0.21715830981943895 1107 40-41 0.13390431876776887 1107 42-43 0.18190446269846205 1107 44-45 0.22756591727931408 1107 46-47 0.28809630860087765 1107 48-49 0.3661647577823146 1107 50-51 0.3462800582406089 1107 52-53 0.2893122693273895 1107 54-55 0.2731486194465802 1107 56-57 0.29245702014654995 1107 58-59 0.23590212075249184 1107 60-61 0.2923113614760098 1107 62-63 0.5257797304687095 1107 64-65 0.6653732540527955 1107 66-67 0.6987760266108509 1107 68-69 0.9937902836980292 1107 70-71 0.8385812752798856 1107 72-73 1.085731227585132 1107 74-75 0.9243618610758446 1107 76-77 0.8729173272505442 1107 78-79 1.1099410698154948 1107 80-81 1.056507074464502 1107 82-83 1.2147164287360894 1107 84-85 1.5690340189213572 1107 86-87 1.8201381492274287 1107 88-89 1.7377017227119431 1107 90-91 1.9563002061092192 1107 92-93 1.7844021094242812 1107 94-95 1.648016995841573 1107 96-97 1.4811140870248458 1107 98-99 1.4467715593289299 1107 100-101 1.4518726711157335 1108 1 0.01271328827800744 1108 2 0.11256162571885397 1108 3 0.10582324310864522 1108 4 0.07411906902650145 1108 5 -0.06421242117409776 1108 6 0.0511013854181499 1108 7 0.11243787911207903 1108 8 0.0732724714820705 1108 9 0.08479435094023557 1108 10-11 0.07237801554941115 1108 12-13 0.04689642041855535 1108 14-15 0.20744549812516055 1108 16-17 0.062496653968558746 1108 18-19 0.09576437428995632 1108 20-21 0.09808162589900604 1108 22-23 0.18414638982341103 1108 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12 8155.0 13 11150.0 14 15195.0 15 19924.0 16 24967.0 17 30576.0 18 32024.0 19 31523.0 20 33540.0 21 36867.0 22 40433.0 23 45322.0 24 50710.0 25 57763.0 26 66675.0 27 77783.0 28 90844.0 29 105587.0 30 121966.0 31 142048.0 32 165247.0 33 195210.0 34 232687.0 35 279910.0 36 318435.0 37 275996.0 38 89333.0 39 1846.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33505528280199 19.308644619848256 14.802708962488786 23.553591134860966 2 16.24792849190386 20.26174912731805 39.093550510376 24.39677187040209 3 19.702802257225155 23.548811958581844 29.54684170512587 27.20154407906713 4 13.660005820416815 16.545433477645506 35.90107279995319 33.893487901984486 5 14.06019592126437 37.057961060495735 34.18927786921858 14.692565149021311 6 33.49867855122866 35.657280702794296 16.64803648888901 14.19600425708804 7 29.63246094641813 31.826589820848184 20.033132780075054 18.507816452658634 8 26.6101960664339 35.706424263747024 18.99829058521643 18.685089084602645 9 26.174211497461684 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28.01995466413505 23.068192139170208 34-35 23.14531388790353 26.515761564328926 27.879606106703815 22.45931844106373 36-37 23.112270701736104 26.13937586702398 27.91607351311662 22.832279918123298 38-39 22.682884804521986 26.55486768835122 27.890997621627907 22.87124988549889 40-41 23.09195982590204 26.22608639113906 27.912794211013452 22.769159571945448 42-43 22.827678651672503 26.27995693735073 27.77458653546454 23.11777787551223 44-45 22.82128756717233 26.483374609356474 27.905905964092153 22.78943185937904 46-47 23.0838978160418 26.70716741665459 27.336208871285706 22.872725896017908 48-49 22.943628001176318 26.622307340188065 27.396935217843062 23.037129440792555 50-51 23.066441855298013 26.819463443231623 27.29027856545464 22.823816136015722 52-53 23.2521445100123 26.67471786084003 27.172378795173717 22.90075883397395 54-55 22.88603570340043 26.742004479655883 27.334562682867976 23.03739713407571 56-57 22.91534811790589 26.864531519545626 27.296798808994343 22.923321553554143 58-59 23.011641216046453 26.57555662037995 27.167197018049794 23.245605145523804 60-61 22.72993227742355 26.768563477534606 27.38477429440831 23.116729950633534 62-63 22.790163266133394 26.81011329927 27.40563524954559 22.994088185051012 64-65 22.570446900978823 26.696005878550118 27.346654146257425 23.386893074213635 66-67 22.589918895932254 26.714818701355913 27.256538652161144 23.43872375055069 68-69 22.84786801213198 26.57067727456464 27.291276515761364 23.290178197542016 70-71 22.755529160132877 26.582589257118205 27.102621999849706 23.55925958289921 72-73 22.806729052020515 26.622337882849635 26.98097080992608 23.589962255203773 74-75 22.600751147352533 26.96619760429808 26.868566039741744 23.56448520860764 76-77 22.728230512980637 26.601580231692363 27.12853445958271 23.54165479574429 78-79 22.726490506640133 26.700072238944557 27.133372059628297 23.440065194787017 80-81 22.717706156131552 26.79028249136353 27.00745851848014 23.48455283402478 82-83 22.692998039889424 26.756474952134944 27.04767116645031 23.50285584152532 84-85 22.714505263065305 26.672507824276508 26.957139964245535 23.65584694841265 86-87 22.736917907874886 26.72473812296007 26.839736280580205 23.698607688584836 88-89 22.665303470490446 26.868145378868213 26.629764510218806 23.836786640422527 90-91 22.615508608829586 26.717363266890448 26.851307075502817 23.815821048777153 92-93 22.61483051673876 26.496690719674298 26.888710510727098 23.99976825285984 94-95 22.485942748404693 26.458934010317286 26.87221443468022 24.1829088065978 96-97 22.522087564002597 26.245452560352405 26.8705928603142 24.361867015330795 98-99 22.329042464950344 26.40889843066725 26.612861591482456 24.64919751289995 100-101 22.68937063934562 26.187320776854 26.70310866651765 24.42019991728273 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 305.0 1 217.5 2 175.5 3 226.0 4 192.0 5 207.5 6 359.0 7 793.0 8 1315.5 9 1529.0 10 1421.0 11 1266.5 12 1302.0 13 1443.5 14 1597.5 15 1734.0 16 1948.5 17 2184.5 18 2369.0 19 2623.5 20 2978.0 21 3530.5 22 4324.5 23 5262.5 24 6543.5 25 8468.5 26 11011.0 27 14165.0 28 17434.0 29 21018.5 30 25209.0 31 30465.5 32 37422.5 33 41697.5 34 44291.5 35 48888.0 36 54621.5 37 60131.0 38 64157.0 39 68211.0 40 72982.5 41 78396.0 42 82517.0 43 86561.0 44 91283.5 45 95623.5 46 98450.0 47 102157.0 48 105061.5 49 104637.5 50 103782.5 51 101212.5 52 98031.0 53 96246.5 54 95388.5 55 90311.5 56 82583.5 57 75818.0 58 69044.0 59 61106.0 60 50887.5 61 41510.0 62 34135.5 63 26429.0 64 19573.5 65 14937.5 66 11178.0 67 8411.5 68 6359.0 69 4644.0 70 3483.5 71 2586.5 72 1919.0 73 1483.5 74 1103.5 75 789.0 76 575.0 77 358.5 78 206.0 79 133.0 80 71.5 81 37.5 82 17.0 83 10.5 84 6.5 85 2.0 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02478074964062177 2 0.011166634097317217 3 0.0015679178013356366 4 0.0 5 1.9120948796776056E-4 6 0.0 7 0.0 8 1.1472569278065634E-4 9 3.059351807484169E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001395829262164652 26-27 0.00833673367539436 28-29 2.2945138556131268E-4 30-31 0.0 32-33 2.103304367645366E-4 34-35 0.010153223811088085 36-37 9.751683886355788E-4 38-39 0.01260070525707542 40-41 0.01196971394678181 42-43 0.005277381867910191 44-45 6.501122590903859E-4 46-47 7.648379518710423E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 9.560474398388028E-5 66-67 8.030798494645942E-4 68-69 9.942893374323548E-4 70-71 5.736284639032817E-5 72-73 1.1472569278065634E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.338466415774324E-4 82-83 0.0011281359790097872 84-85 0.005908373178203801 86-87 0.004149245888900404 88-89 0.0 90-91 0.0011663778766033393 92-93 5.353865663097296E-4 94-95 5.736284639032817E-5 96-97 0.0 98-99 0.0 100-101 5.736284639032817E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2614933.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.01694620286377 #Duplication Level Percentage of deduplicated Percentage of total 1 75.17188098944044 42.10899213353564 2 14.073931489282662 15.767573261958745 3 4.71131956210729 7.917412033651913 4 2.071986674487526 4.642654643112735 5 1.079100848679575 3.02239670939742 6 0.6652663821963656 2.235971468524057 7 0.43821427895228554 1.7183197982577842 8 0.31385021169210414 1.4064744339291204 9 0.2183620424383271 1.1008777305613702 >10 1.14589261134432 11.431643741848035 >50 0.0726214236541707 2.768166219294475 >100 0.033675110005047026 3.4957428641038555 >500 0.0027357022595791616 1.0741373267075645 >1k 0.001094280903816091 1.0982735222885025 >5k 6.839255648850569E-5 0.21136411282888576 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5517 0.21098054902362698 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4839 0.18505254245519867 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3192 0.12206813711861833 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.824189759355211E-5 2 0.0 0.0 0.0 0.0 3.824189759355211E-5 3 0.0 0.0 0.0 0.0 3.824189759355211E-5 4 0.0 0.0 0.0 0.0 3.824189759355211E-5 5 0.0 0.0 0.0 0.0 3.824189759355211E-5 6 0.0 0.0 0.0 0.0 7.648379518710423E-5 7 0.0 0.0 0.0 0.0 7.648379518710423E-5 8 0.0 0.0 0.0 0.0 7.648379518710423E-5 9 0.0 0.0 0.0 0.0 1.1472569278065632E-4 10-11 0.0 0.0 0.0 3.824189759355211E-5 1.1472569278065632E-4 12-13 0.0 0.0 0.0 3.824189759355211E-5 1.9120948796776056E-4 14-15 0.0 0.0 0.0 3.824189759355211E-5 2.868142319516409E-4 16-17 0.0 0.0 0.0 5.736284639032817E-5 3.059351807484169E-4 18-19 0.0 0.0 0.0 7.648379518710423E-5 3.44177078341969E-4 20-21 0.0 0.0 0.0 1.338466415774324E-4 3.44177078341969E-4 22-23 0.0 0.0 0.0 1.9120948796776056E-4 4.206608735290732E-4 24-25 0.0 0.0 0.0 3.8241897593552113E-4 4.589027711226253E-4 26-27 0.0 0.0 0.0 8.222007982613703E-4 5.353865663097296E-4 28-29 0.0 0.0 0.0 0.002734295677938976 5.353865663097296E-4 30-31 0.0 0.0 0.0 0.010057619067104204 5.353865663097296E-4 32-33 0.0 0.0 0.0 0.026922295905860684 5.927494127000578E-4 34-35 0.0 0.0 0.0 0.05317535860383421 7.265960542774901E-4 36-37 0.0 0.0 0.0 0.09979223177037423 7.265960542774901E-4 38-39 0.0 0.0 0.0 0.1719355715806103 7.457170030742662E-4 40-41 0.0 0.0 0.0 0.24977695413228562 7.648379518710423E-4 42-43 0.0 0.0 0.0 0.3299893343347612 7.839589006678183E-4 44-45 0.0 0.0 0.0 0.43060376690339675 8.413217470581464E-4 46-47 0.0 0.0 0.0 0.5468208936902016 8.795636446516985E-4 48-49 0.0 0.0 0.0 0.6742046545743237 9.560474398388028E-4 50-51 0.0 0.0 0.0 0.8081277799469432 9.560474398388028E-4 52-53 0.0 0.0 0.0 0.9399476009519173 0.0011090150302130112 54-55 0.0 0.0 0.0 1.0876378094582155 0.0011090150302130112 56-57 0.0 0.0 0.0 1.2615619597136907 0.0011090150302130112 58-59 0.0 0.0 0.0 1.4495017654372022 0.0011281359790097872 60-61 0.0 0.0 0.0 1.6528530558909158 0.0011472569278065633 62-63 0.0 0.0 0.0 1.8546364285432935 0.0012619826205872197 64-65 0.0 0.0 0.0 2.06383872932882 0.0013002245181807717 66-67 0.0 0.0 0.0 2.315049754620864 0.0013193454669775478 68-69 0.0 0.0 0.0 2.5913092228366845 0.0013384664157743238 70-71 0.0 0.0 0.0 2.8830375386290967 0.0013384664157743238 72-73 0.0 0.0 0.0 3.1826628062745774 0.0014914340061485325 74-75 0.0 0.0 0.0 3.487966995712701 0.0015296759037420845 76-77 0.0 0.0 0.0 3.817956330047462 0.0015296759037420845 78-79 0.0 0.0 0.0 4.191178129611734 0.0015296759037420845 80-81 0.0 0.0 0.0 4.593635859886277 0.0015679178013356366 82-83 3.824189759355211E-5 0.0 0.0 5.002499108007738 0.0016444015965227407 84-85 5.736284639032817E-5 0.0 0.0 5.408608939502465 0.0016444015965227407 86-87 7.648379518710423E-5 0.0 0.0 5.830818609884077 0.0016826434941162927 88-89 7.648379518710423E-5 0.0 0.0 6.286509061608845 0.001720885391709845 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4730 0.0 42.49157 1 GTATCAA 7915 0.0 38.299515 1 ATCAACG 10205 0.0 29.277115 3 TCAACGC 10255 0.0 29.087494 4 TATCAAC 10345 0.0 29.067347 2 CAACGCA 10480 0.0 28.553648 5 AACGCAG 10845 0.0 27.592115 6 ACGCAGA 12270 0.0 24.232811 7 CGCAGAG 12540 0.0 23.673174 8 GCAGAGT 13710 0.0 21.236784 9 AGAGTAC 11775 0.0 20.975298 10-11 TACATGG 8865 0.0 20.791468 2 GTACATG 9235 0.0 20.631508 1 TAAGGTG 1345 0.0 20.48274 5 AGTACTT 7455 0.0 19.878 12-13 GAGTACT 6925 0.0 19.4446 12-13 ACATGGG 9240 0.0 19.020433 3 GTAAGGT 1210 0.0 18.842485 4 CAGAGTA 13150 0.0 18.42087 10-11 GTACTTT 7355 0.0 18.27551 14-15 >>END_MODULE