FastQCFastQC Report
Thu 26 May 2016
SRR938435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226989
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101120.313357126411029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76310.23647431088237364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56480.17502383801122348No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45050.13960382263466037No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA43630.13520343577248017No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42140.13058612843117842No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38550.1194612067162299No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC36290.112457774104591No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA33950.10520643237395602No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32790.10161175014851305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62000.045.1026731
GTATCAA105750.040.7890471
ATCAACG137950.030.819033
TCAACGC139150.030.587434
TATCAAC140350.030.3936732
CAACGCA142250.029.8539875
AACGCAG147800.028.9035686
ACGCAGA166300.025.5207127
GTACATG126950.025.3239021
CGCAGAG167800.025.1788968
TACATGG125750.024.8840482
TAAGGTG20050.024.6673285
ACGACCG1351.3315463E-524.6585455
ACATGGG127100.023.4598463
GCAGAGT182300.022.6019449
AGGTAAG20400.022.3791832
GTATAGG7950.022.1335581
GTAAGGT21950.021.6654974
AGAGTAC161050.021.32049210-11
GAGTACT99200.021.29363812-13
Produced by FastQC (version 0.11.5)