FastQCFastQC Report
Thu 26 May 2016
SRR938435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226989
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103660.3212282409391541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81320.25199961945950233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62030.1922225331415756No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA46450.14394223221709151No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA44810.13886009527767215No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC42750.13247643546352345No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40220.12463630957527279No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38770.12014295679346908No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG36210.11220986498559495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70150.042.207891
GTATCAA116250.038.266271
ATCAACG145850.030.1579723
TATCAAC148800.029.6719472
TCAACGC148400.029.6717664
CAACGCA151850.028.966355
AACGCAG156850.028.0913476
ACGCAGA178850.024.6358857
CGCAGAG180650.024.3378458
TACATGG119300.023.105792
GTACATG122250.023.0536171
ACATGGG118700.021.9294343
GCAGAGT199100.021.8917549
TAAGGTG19650.021.7559225
GAGTACT111600.021.2297812-13
AGTACTT118750.020.01149612-13
AGAGTAC174150.019.87527510-11
GTACTTT117050.019.85593614-15
CAGAGTA187700.019.18670710-11
GTATAGG8700.019.1197431
Produced by FastQC (version 0.11.5)