##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938435_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3226989 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.786545910134805 31.0 31.0 34.0 30.0 34.0 2 32.010533038693346 31.0 31.0 34.0 30.0 34.0 3 32.07252767208069 33.0 31.0 34.0 30.0 34.0 4 35.75020708158596 37.0 35.0 37.0 33.0 37.0 5 35.64831364470099 37.0 35.0 37.0 33.0 37.0 6 35.64893155818009 37.0 35.0 37.0 33.0 37.0 7 35.59139804938908 37.0 35.0 37.0 33.0 37.0 8 35.565441654743786 37.0 35.0 37.0 33.0 37.0 9 37.15241669556357 39.0 37.0 39.0 33.0 39.0 10-11 37.05097708730956 39.0 37.0 39.0 33.0 39.0 12-13 36.94579141732432 39.0 37.0 39.0 33.0 39.0 14-15 37.981000555006545 40.0 37.0 41.0 33.0 41.0 16-17 37.88783336416703 40.0 37.0 41.0 33.0 41.0 18-19 38.143341982262726 40.0 37.0 41.0 33.0 41.0 20-21 38.11713210054326 40.0 37.0 41.0 33.0 41.0 22-23 38.009600900405914 40.0 37.0 41.0 33.0 41.0 24-25 37.87996937702607 40.0 37.0 41.0 32.0 41.0 26-27 37.684905960324 40.0 37.0 41.0 32.0 41.0 28-29 37.57386917030086 39.5 37.0 41.0 32.0 41.0 30-31 37.31320280298445 39.0 36.0 41.0 31.0 41.0 32-33 37.11012866793162 39.0 36.0 41.0 31.0 41.0 34-35 37.008328196966275 39.0 36.0 41.0 31.0 41.0 36-37 36.957810206356456 39.0 36.0 41.0 31.0 41.0 38-39 36.79055553024817 39.0 35.0 41.0 30.0 41.0 40-41 36.620256375215405 39.0 35.0 40.5 30.0 41.0 42-43 36.40450804139711 39.0 35.0 40.0 30.0 41.0 44-45 36.1085539801964 38.5 35.0 40.0 29.5 41.0 46-47 35.73264210073229 38.0 35.0 40.0 28.5 41.0 48-49 35.37232788831942 38.0 34.0 40.0 27.0 41.0 50-51 34.9995808786457 38.0 34.0 40.0 26.0 41.0 52-53 34.51199632226822 38.0 33.0 40.0 24.5 41.0 54-55 34.177095893416436 37.5 33.0 40.0 24.0 41.0 56-57 33.84528239792574 37.0 33.0 40.0 23.0 41.0 58-59 33.36936769849541 37.0 32.0 39.0 22.5 41.0 60-61 32.970630516558934 36.0 31.5 39.0 22.0 40.0 62-63 32.49263911342741 35.5 31.0 39.0 20.0 40.0 64-65 31.97170597730578 35.0 31.0 38.5 18.0 40.0 66-67 31.462315954594203 35.0 30.0 38.0 17.0 40.0 68-69 30.9531316964514 34.0 29.0 37.5 13.0 39.5 70-71 30.362200490922035 34.0 29.0 37.0 8.0 39.0 72-73 29.849806429461026 34.0 28.5 36.0 7.5 39.0 74-75 29.25487660478545 34.0 28.0 36.0 7.0 38.5 76-77 28.033298068261157 31.5 26.0 34.5 2.0 36.5 78-79 28.725225744494324 33.0 28.0 35.0 2.0 37.0 80-81 28.340601099043102 33.0 26.5 35.0 2.0 37.0 82-83 27.884160280682703 33.0 26.0 35.0 2.0 36.0 84-85 27.307227108614253 32.5 26.0 35.0 2.0 36.0 86-87 26.679262619116457 32.0 25.0 34.5 2.0 35.0 88-89 25.902030654582337 31.5 23.5 34.0 2.0 35.0 90-91 25.26110222253624 31.0 20.5 34.0 2.0 35.0 92-93 24.40688425030268 31.0 18.0 34.0 2.0 35.0 94-95 23.554424263609203 30.5 6.0 34.0 2.0 35.0 96-97 22.442112445998422 30.0 2.0 34.0 2.0 35.0 98-99 21.02911894028768 29.0 2.0 34.0 2.0 35.0 100-101 17.04628633689176 22.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27760245073912415 1101 2 0.30053540669624823 1101 3 0.3455581506065357 1101 4 0.20641374282955383 1101 5 0.1017971568987619 1101 6 0.2081332821399684 1101 7 0.2188717672039715 1101 8 0.22890367798854783 1101 9 0.28142185705281264 1101 10-11 0.2984717759432769 1101 12-13 0.3598229616073141 1101 14-15 0.4043962998253363 1101 16-17 0.4650042895178004 1101 18-19 0.39022382708869685 1101 20-21 0.419403311981533 1101 22-23 0.46630757586263627 1101 24-25 0.5034630816974541 1101 26-27 0.4896767903020418 1101 28-29 0.4604329183325646 1101 30-31 0.47301406439747495 1101 32-33 0.4130384515902392 1101 34-35 0.4427378441198826 1101 36-37 0.561895887720965 1101 38-39 0.6839642497666247 1101 40-41 0.5671195214920672 1101 42-43 0.5993861563092509 1101 44-45 0.5637406191160821 1101 46-47 0.492498454345764 1101 48-49 0.6007526735467863 1101 50-51 0.6713676004059508 1101 52-53 0.6644503260988515 1101 54-55 0.6961467748820098 1101 56-57 0.5596482077201514 1101 58-59 0.7795655579702512 1101 60-61 0.6515523974000956 1101 62-63 0.8280603491791609 1101 64-65 0.8146330562813624 1101 66-67 0.7320508253339852 1101 68-69 0.8944914808839073 1101 70-71 0.8732696318553472 1101 72-73 0.7957944906767445 1101 74-75 0.801282013521476 1101 76-77 0.7048666256309026 1101 78-79 0.8109667530823721 1101 80-81 0.7855846312473886 1101 82-83 0.8258311498423545 1101 84-85 0.9933459646509171 1101 86-87 1.171493505203081 1101 88-89 1.1114522102641864 1101 90-91 1.2441470750880477 1101 92-93 1.6123593492597088 1101 94-95 1.5889344852007667 1101 96-97 1.6382426428524042 1101 98-99 1.713911334367264 1101 100-101 1.7816329242754634 1102 1 0.34353146548640723 1102 2 0.2976323576418096 1102 3 0.29041604404316246 1102 4 0.19651008089778088 1102 5 0.13591296728413482 1102 6 0.1609576559959578 1102 7 0.17301592367964957 1102 8 0.31152167948230414 1102 9 0.26895467435247156 1102 10-11 0.3411608296910984 1102 12-13 0.38649746085447134 1102 14-15 0.5317089766030421 1102 16-17 0.5442276761110421 1102 18-19 0.4385697159807407 1102 20-21 0.34378340567653964 1102 22-23 0.5373402536104237 1102 24-25 0.5050153651484877 1102 26-27 0.5240581911398507 1102 28-29 0.43023802251965293 1102 30-31 0.4446650967319954 1102 32-33 0.44682845252332726 1102 34-35 0.3615457792165344 1102 36-37 0.4815642043471229 1102 38-39 0.4793435308134235 1102 40-41 0.44986090000157475 1102 42-43 0.27120560659404447 1102 44-45 0.3595400972454854 1102 46-47 0.49188131236005006 1102 48-49 0.5171582713241492 1102 50-51 0.5798231598116814 1102 52-53 0.49046037136791654 1102 54-55 0.5529896674163766 1102 56-57 0.49983465848628583 1102 58-59 0.7583947772977169 1102 60-61 0.8037919378941893 1102 62-63 0.707980323677539 1102 64-65 0.7517987432075053 1102 66-67 0.6338585051678507 1102 68-69 0.6954888041185363 1102 70-71 0.7505202912842286 1102 72-73 0.8036855432021035 1102 74-75 0.9939577903157435 1102 76-77 0.8662805204766073 1102 78-79 1.1149700936954225 1102 80-81 0.9932247781241976 1102 82-83 1.0922910783112236 1102 84-85 1.138412353529187 1102 86-87 0.9891627565596579 1102 88-89 0.9747156710603839 1102 90-91 1.1228238252515403 1102 92-93 1.3866475935655203 1102 94-95 1.4681327565660816 1102 96-97 1.305702381415621 1102 98-99 1.708702390879818 1102 100-101 1.8380971256214949 1103 1 0.3185130343326392 1103 2 0.28842117506982135 1103 3 0.30810955218233005 1103 4 0.21763335161013941 1103 5 0.1855246796506762 1103 6 0.2496861590586974 1103 7 0.2908907172717008 1103 8 0.2793418137925343 1103 9 0.36951866466181116 1103 10-11 0.39378744138090127 1103 12-13 0.38645791202274893 1103 14-15 0.5390856326853992 1103 16-17 0.4566819427808255 1103 18-19 0.5392352748806317 1103 20-21 0.516496149928031 1103 22-23 0.5293401541391134 1103 24-25 0.5080572295842813 1103 26-27 0.5253014326340022 1103 28-29 0.5046437064457976 1103 30-31 0.33605406768137414 1103 32-33 0.4512509806564253 1103 34-35 0.494501011736709 1103 36-37 0.5356068339928868 1103 38-39 0.6035257638403309 1103 40-41 0.613339890883168 1103 42-43 0.6822096994986495 1103 44-45 0.6074953427488552 1103 46-47 0.6385897010467687 1103 48-49 0.9370557080205515 1103 50-51 0.9683832907143213 1103 52-53 0.8729620407081242 1103 54-55 0.8360339147691533 1103 56-57 0.8738138542028722 1103 58-59 0.9148510042313092 1103 60-61 0.8524263147374356 1103 62-63 0.9127280710420038 1103 64-65 0.9825969864404094 1103 66-67 0.9661425960111174 1103 68-69 1.063469860228139 1103 70-71 0.9691578679874873 1103 72-73 1.0497387994991065 1103 74-75 1.209405394205831 1103 76-77 1.0311754793787777 1103 78-79 1.3326800705273953 1103 80-81 1.1180535246918915 1103 82-83 1.2744096532989069 1103 84-85 1.2395631346485736 1103 86-87 1.1354318136535895 1103 88-89 1.1542984268908896 1103 90-91 1.2876229081980277 1103 92-93 1.6615162185385302 1103 94-95 1.6615924084684472 1103 96-97 1.6480650825528897 1103 98-99 1.7214999334192775 1103 100-101 1.9122994726249374 1104 1 0.13696452985372787 1104 2 0.20769731523304102 1104 3 0.15850413312172407 1104 4 0.10898444237550109 1104 5 0.07757711388861566 1104 6 0.07541849979182302 1104 7 0.12346911038976316 1104 8 0.18843686575271335 1104 9 0.19832121024914073 1104 10-11 0.21620853522448868 1104 12-13 0.22123778418161777 1104 14-15 0.33145615069361867 1104 16-17 0.35316685630089495 1104 18-19 0.24234342737511838 1104 20-21 0.25334932752699757 1104 22-23 0.3269532647622171 1104 24-25 0.26546088872274964 1104 26-27 0.3344269516195695 1104 28-29 0.25179390744791164 1104 30-31 0.24175432488841864 1104 32-33 0.30412921172316487 1104 34-35 0.30868268119211706 1104 36-37 0.31025130415612523 1104 38-39 0.2788229391486894 1104 40-41 0.30812879710263275 1104 42-43 0.4806147234910725 1104 44-45 0.32859466066581433 1104 46-47 0.2031969916941634 1104 48-49 0.36045163203126407 1104 50-51 0.5602777721987948 1104 52-53 0.3975456054819375 1104 54-55 0.21322844343021785 1104 56-57 0.3132238830419354 1104 58-59 0.46423667664686974 1104 60-61 0.40961547225968786 1104 62-63 0.4893517800626981 1104 64-65 0.5106884150314919 1104 66-67 0.4158720041139148 1104 68-69 0.5489533114456506 1104 70-71 0.4897609431220964 1104 72-73 0.5852462116133417 1104 74-75 0.675911012692179 1104 76-77 0.38581136833091634 1104 78-79 0.7473060483813363 1104 80-81 0.7378756350965965 1104 82-83 0.8859235520520805 1104 84-85 0.9155084866072087 1104 86-87 1.0217797774646087 1104 88-89 1.1869560400072459 1104 90-91 1.3904905345616605 1104 92-93 1.3916260947271901 1104 94-95 1.2847263389898735 1104 96-97 1.365227762711033 1104 98-99 1.6232682819918587 1104 100-101 2.037697077337782 1105 1 0.2132264018025083 1105 2 0.2903983609297498 1105 3 0.2552712654859235 1105 4 0.1761909937825763 1105 5 0.08301832547147825 1105 6 0.2251245268627997 1105 7 0.20076777077456143 1105 8 0.22559904998082914 1105 9 0.3032638126833618 1105 10-11 0.21440628867114242 1105 12-13 0.3188702978814888 1105 14-15 0.5012110480652652 1105 16-17 0.30663511274007504 1105 18-19 0.3679225556781631 1105 20-21 0.39486106104379104 1105 22-23 0.21706743395622397 1105 24-25 0.2804324076572797 1105 26-27 0.20611311125142606 1105 28-29 0.24424918284953634 1105 30-31 0.39722065561712583 1105 32-33 0.3863755909012525 1105 34-35 0.30543261388115894 1105 36-37 0.34612714137026757 1105 38-39 0.26321341749559934 1105 40-41 0.31704137666927323 1105 42-43 0.32047390931506214 1105 44-45 0.4005748022982516 1105 46-47 0.29393666299893795 1105 48-49 0.327640530185235 1105 50-51 0.39594072714653095 1105 52-53 0.3098822357825384 1105 54-55 0.33209411941011524 1105 56-57 0.2238816853443666 1105 58-59 0.4123913129753447 1105 60-61 0.514057823593177 1105 62-63 0.3855277461655575 1105 64-65 0.42207779317977767 1105 66-67 0.42335336387523625 1105 68-69 0.56260029210436 1105 70-71 0.6205286401001082 1105 72-73 0.5960538397781399 1105 74-75 0.4898424139286668 1105 76-77 0.4293463224977714 1105 78-79 0.8416449966466004 1105 80-81 0.8057622358964878 1105 82-83 0.939611349281698 1105 84-85 1.0174441848595883 1105 86-87 1.1092753233293209 1105 88-89 1.1226219522830938 1105 90-91 1.226494942976295 1105 92-93 1.5009280800196514 1105 94-95 1.719125861680741 1105 96-97 1.913025442278908 1105 98-99 1.879092676211318 1105 100-101 1.7742649565452666 1106 1 0.021982545119222152 1106 2 0.05686316302400485 1106 3 0.019989725038108475 1106 4 0.02220095861676441 1106 5 -0.0230848679832647 1106 6 0.007768631775313395 1106 7 0.04791509624730139 1106 8 0.04726489634976616 1106 9 -0.21403073839978504 1106 10-11 0.032051784523282834 1106 12-13 -0.0434481666638149 1106 14-15 0.024291194720227338 1106 16-17 0.0563706558841659 1106 18-19 0.041854028718894654 1106 20-21 0.14060665818487905 1106 22-23 0.136996121551185 1106 24-25 0.18037213360650384 1106 26-27 0.12460815023339933 1106 28-29 0.13364184154148973 1106 30-31 0.06943191081531808 1106 32-33 0.14127740482919648 1106 34-35 0.10878246016449822 1106 36-37 0.3173978182228865 1106 38-39 0.13217317297554843 1106 40-41 0.31218601655856304 1106 42-43 0.2853256672487632 1106 44-45 0.28726056763602514 1106 46-47 -0.4285547417074298 1106 48-49 -0.1383185405244305 1106 50-51 0.29244224648059713 1106 52-53 0.284227245875762 1106 54-55 0.38106740980264675 1106 56-57 0.26234018541212833 1106 58-59 0.059381087785787656 1106 60-61 0.15128427213197426 1106 62-63 -0.052602456483647586 1106 64-65 0.1596781013264028 1106 66-67 -0.018206574923418373 1106 68-69 -0.11075304936062125 1106 70-71 -0.05538759680187866 1106 72-73 0.35802100290326067 1106 74-75 0.3305934678329301 1106 76-77 0.32613432189859637 1106 78-79 0.507137924253545 1106 80-81 -0.13747414181138495 1106 82-83 0.480786104797307 1106 84-85 0.5583288046337564 1106 86-87 0.7965578552674337 1106 88-89 0.867104290916263 1106 90-91 0.9951445010854734 1106 92-93 0.9481058100061723 1106 94-95 1.3252171964834787 1106 96-97 1.4371165167262774 1106 98-99 1.5412172116731462 1106 100-101 1.621497789140328 1107 1 0.1024647654623898 1107 2 0.13250717319872507 1107 3 0.13486015746358504 1107 4 0.08846223727665858 1107 5 0.12410194803321417 1107 6 0.15519398296660114 1107 7 0.0749516925191216 1107 8 0.08983968434749556 1107 9 0.1837726849540431 1107 10-11 0.15572331236631243 1107 12-13 0.14141955157585784 1107 14-15 0.353158213535103 1107 16-17 0.33017580518178136 1107 18-19 0.19775338114200025 1107 20-21 0.2283675070986888 1107 22-23 0.22746985136442532 1107 24-25 0.2496330225371466 1107 26-27 0.30482451955753476 1107 28-29 0.29615719582972844 1107 30-31 0.17676925975173674 1107 32-33 0.20155416478729649 1107 34-35 0.36488694239875485 1107 36-37 0.3613522303406853 1107 38-39 0.3692051934084972 1107 40-41 0.42086479962900825 1107 42-43 0.3272256495021608 1107 44-45 0.4053947214886051 1107 46-47 0.42651652306835075 1107 48-49 0.49920446915964334 1107 50-51 0.6568237774901675 1107 52-53 0.5710978070510819 1107 54-55 0.5388483367318955 1107 56-57 0.45616036712338826 1107 58-59 0.5667489713124141 1107 60-61 0.45631873881186635 1107 62-63 0.7435488802528525 1107 64-65 0.6280105995256484 1107 66-67 0.41486969369746163 1107 68-69 0.6927795678452284 1107 70-71 0.8067310687930309 1107 72-73 0.951717567370391 1107 74-75 0.86416481438226 1107 76-77 0.8792720772669433 1107 78-79 1.219010660257215 1107 80-81 1.2088015081143233 1107 82-83 1.2912122319673536 1107 84-85 1.4010338213538382 1107 86-87 1.4444306121494606 1107 88-89 1.51474703795067 1107 90-91 1.4705513679755349 1107 92-93 1.649281783198365 1107 94-95 1.7005182797219902 1107 96-97 1.5130456034027588 1107 98-99 1.5113083630794755 1107 100-101 1.673926724448755 1108 1 0.07211638937071285 1108 2 0.05956858337648896 1108 3 0.1787860779521111 1108 4 0.11679827086113903 1108 5 0.0765631963706852 1108 6 0.1193363080488723 1108 7 0.045969259007343055 1108 8 0.04491956459266788 1108 9 0.05116160326314656 1108 10-11 0.1515441275450442 1108 12-13 0.19426058280964043 1108 14-15 0.3487534397707108 1108 16-17 0.4118929202299668 1108 18-19 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2316 70-71 -0.9351443421001555 2316 72-73 -1.0069089108672777 2316 74-75 -0.9876871256457882 2316 76-77 -1.1122658187762724 2316 78-79 -1.1990573229455208 2316 80-81 -1.2300957625515885 2316 82-83 -1.2664146768472655 2316 84-85 -1.410488133819225 2316 86-87 -1.5354065423559007 2316 88-89 -1.6066538523164873 2316 90-91 -1.5839187417875742 2316 92-93 -1.8213493657448687 2316 94-95 -1.4398276987199132 2316 96-97 -1.5514803572242784 2316 98-99 -1.6148293837922942 2316 100-101 -1.8804561900810413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 42.0 5 103.0 6 231.0 7 606.0 8 1496.0 9 2520.0 10 4285.0 11 6764.0 12 9255.0 13 12943.0 14 16967.0 15 22327.0 16 28154.0 17 34514.0 18 32984.0 19 29448.0 20 29169.0 21 31169.0 22 34106.0 23 38387.0 24 44252.0 25 51736.0 26 61713.0 27 74109.0 28 87890.0 29 104646.0 30 123923.0 31 147983.0 32 177342.0 33 218001.0 34 275232.0 35 356426.0 36 457777.0 37 491126.0 38 212598.0 39 6745.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.857733060301655 19.757258808246483 15.29351318386006 23.0914949475918 2 15.739946101405259 20.804752154517573 39.605669646012984 23.849632098064188 3 19.06944833093636 24.537083950394624 30.25706006435101 26.136407654318006 4 13.145787960785723 17.28518978068713 36.87658584413701 32.69243641439014 5 13.435125390245945 37.830279054915 34.46745186062445 14.267143694214592 6 32.08119524497619 36.97916973325272 16.986960282145542 13.95267473962554 7 28.612183059812104 32.85186283560309 20.519220858825364 18.016733245759436 8 26.442916291316763 35.405977522699956 19.490212083152436 18.660894102830845 9 26.37185934008452 16.687568504262025 19.90518095971198 37.03539119594148 10-11 24.79058651888804 26.85748231555794 27.508879020040045 20.843052145513976 12-13 25.960051304792174 24.04357436607314 27.662691134057166 22.333683195077516 14-15 22.39919317977223 25.680440807204484 25.961042941268158 25.959323071755126 16-17 21.89807588436155 28.770999839168958 27.013866486684645 22.31705778978484 18-19 22.006288214803334 27.18856494397719 29.277602123837422 21.527544717382057 20-21 22.516361227137743 26.40326632659733 29.464339667721212 21.616032778543715 22-23 22.20360837920427 26.399950542130764 29.37010321386283 22.02633786480214 24-25 22.10581952208039 26.651919237059147 29.40669590740234 21.835565333458124 26-27 21.66574809621949 26.96518940808994 29.189343632478444 22.179718863212127 28-29 21.83807991789245 27.262272826013863 29.16655691516576 21.73309034092793 30-31 21.970201130515665 26.891698607455467 28.922805839411986 22.215294422616882 32-33 21.62272381677518 26.85355063898068 29.003752894932113 22.519972649312024 34-35 22.473606322395693 26.917863092240346 28.76200364157595 21.846526943788014 36-37 22.52680356450988 26.413607129019763 28.82143355288648 22.238155753583882 38-39 21.951885416765123 26.892370120236098 28.8047868213661 22.350957641632675 40-41 22.379999101319974 26.680926756028516 28.94388053133682 21.995193611314694 42-43 22.193479831208567 26.69560738792559 28.671069598111647 22.439843182754192 44-45 22.101776144052657 26.93123241048387 28.891790365973502 22.075201079489972 46-47 22.642794877825736 27.111263781810223 28.061298008762968 22.184643331601066 48-49 22.257792016024847 27.228369851895994 28.020036634770058 22.4938014973091 50-51 22.48602482017967 27.281295679446764 28.001661620138513 22.231017880235058 52-53 22.753517288097356 27.040129358978295 27.99250322824156 22.213850124682793 54-55 22.18099809726862 27.28103094572567 28.179374267882267 22.35859668912344 56-57 22.30086622545041 27.460893111194366 27.882602016926615 22.355638646428606 58-59 22.218711085728987 26.976800411004444 27.971920895592937 22.83256760767363 60-61 21.82147196659177 27.35543876970142 28.22570823761717 22.597381026089646 62-63 22.03070326115809 27.309830085935598 28.31332890460586 22.34613774830045 64-65 21.7416921057823 27.30602826070213 28.105192802901108 22.84708683061446 66-67 21.74702900506728 27.408879003564234 27.98468504680051 22.859406944567976 68-69 22.327525648597287 26.951502482532234 28.086885761365416 22.63408610750506 70-71 22.105262342403204 27.012087133965608 27.805071916975134 23.077578606656058 72-73 22.0062466958332 27.20205479664306 27.823207166067654 22.968491341456083 74-75 22.054848125817024 27.523419054032168 27.545301892509077 22.876430927641728 76-77 22.10034149512204 27.090411643543522 27.977812195820302 22.831434665514138 78-79 22.09608233591775 27.302291007698688 27.82530647823127 22.77632017815229 80-81 22.313779710183564 27.3067194272546 27.581753519796305 22.79774734276554 82-83 22.298625987706476 27.380241050056814 27.64018848397683 22.680944478259878 84-85 22.116270910545914 27.479573727669955 27.63895784944717 22.765197512336965 86-87 22.330080839210424 27.486168325558502 27.293739764932816 22.890011070298254 88-89 22.106215308157072 27.53554978613837 27.13150621797542 23.22672868772914 90-91 21.942724956019823 27.408463814440005 27.51293755644271 23.135873673097464 92-93 22.113317413734745 27.19047911296592 27.607469688910708 23.088733784388626 94-95 21.85828495760873 26.86427354581456 27.80660827689843 23.470833219678276 96-97 22.046782383902364 26.60788578711007 27.773702087147996 23.571629741839573 98-99 21.750730279658782 26.912287843740696 27.343301277971964 23.993680598628565 100-101 21.86924739700889 26.595379523224423 27.910647620189934 23.624725459576755 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 977.0 1 645.5 2 421.5 3 525.0 4 471.0 5 496.0 6 893.0 7 1982.0 8 2984.0 9 3180.5 10 2642.0 11 2090.0 12 2077.0 13 2189.5 14 2400.5 15 2608.5 16 2822.5 17 3172.0 18 3549.0 19 4026.5 20 4668.5 21 5487.0 22 6573.0 23 8103.0 24 10002.5 25 12532.0 26 16665.5 27 21604.5 28 25474.5 29 30170.0 30 36371.0 31 44568.5 32 57637.0 33 64697.5 34 66705.0 35 72610.0 36 81367.5 37 89693.5 38 93663.5 39 96383.5 40 100177.5 41 105242.5 42 108019.0 43 110135.5 44 113529.5 45 118865.0 46 121477.0 47 122274.5 48 123635.5 49 120979.5 50 116521.0 51 111226.5 52 107089.0 53 106690.5 54 106801.0 55 99967.5 56 90436.5 57 81736.0 58 74252.5 59 65658.0 60 53120.5 61 43879.0 62 37343.5 63 27646.0 64 18507.0 65 13521.0 66 10377.0 67 8231.0 68 6410.0 69 5028.5 70 3921.0 71 2610.0 72 1822.0 73 1562.5 74 1166.5 75 846.0 76 633.0 77 386.5 78 226.5 79 154.0 80 108.0 81 59.0 82 28.5 83 22.5 84 11.0 85 6.5 86 3.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.028523183686093E-4 2 0.05184399451005256 3 0.0 4 3.09886398745084E-5 5 0.04521242557690776 6 6.19772797490168E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0065695916533957815 26-27 0.0016888808731607083 28-29 6.19772797490168E-5 30-31 0.0016888808731607083 32-33 4.1834663830586346E-4 34-35 0.011279864914321059 36-37 0.02290060486726171 38-39 0.004013028863748838 40-41 0.0010536137557332857 42-43 0.0014874547139764034 44-45 0.008676819164862353 46-47 0.0 48-49 0.0 50-51 5.577955177411512E-4 52-53 0.0 54-55 0.0015184433538509119 56-57 0.0 58-59 0.023133019666320524 60-61 0.0 62-63 0.01017976819877601 64-65 0.015323882417944405 66-67 0.006445637093897749 68-69 0.016904303051544334 70-71 1.239545594980336E-4 72-73 9.296591962352521E-5 74-75 0.02220336047008527 76-77 0.05000017043751931 78-79 0.030523810276390777 80-81 0.026138917734147837 82-83 0.03546649833637487 84-85 0.01571124041637576 86-87 0.010443171637709333 88-89 0.08391723678016876 90-91 0.02539519037715964 92-93 0.02407817318249303 94-95 0.026433309812955667 96-97 0.037914600886461036 98-99 0.0521693752287349 100-101 0.01427026866221112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3226989.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.186773353467295 #Duplication Level Percentage of deduplicated Percentage of total 1 68.90098264300985 31.823140691638805 2 15.820022697942186 14.61351605593128 3 6.245321758605311 8.653537817525438 4 3.007844646515725 5.55690555884247 5 1.7382364552630152 4.0141766596983635 6 1.0249795109103335 2.8404297817417996 7 0.6846801726453265 2.213621756750779 8 0.46579864354679934 1.7210989102278818 9 0.3426844744895617 1.4244741139501291 >10 1.5887019642739815 13.013874080304852 >50 0.11148538402958025 3.4966149256648626 >100 0.06167676463494934 5.337424569222215 >500 0.003893126370198288 1.2325110242186414 >1k 0.003490389157919978 3.2928964408723354 >5k 1.3424573684307607E-4 0.44441205166740344 >10k+ 6.712286842153803E-5 0.32136556174288833 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10366 0.3212282409391541 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8132 0.25199961945950233 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6203 0.1922225331415756 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4645 0.14394223221709151 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4481 0.13886009527767215 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4275 0.13247643546352345 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4022 0.12463630957527279 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3877 0.12014295679346908 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3621 0.11220986498559495 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.09886398745084E-5 0.0 10-11 0.0 0.0 0.0 3.09886398745084E-5 0.0 12-13 0.0 0.0 0.0 3.09886398745084E-5 0.0 14-15 0.0 0.0 0.0 3.09886398745084E-5 0.0 16-17 0.0 0.0 0.0 3.09886398745084E-5 0.0 18-19 0.0 0.0 0.0 4.64829598117626E-5 0.0 20-21 0.0 0.0 0.0 1.8593183924705043E-4 0.0 22-23 0.0 0.0 0.0 5.732898376784054E-4 0.0 24-25 0.0 0.0 0.0 0.0010226251158587773 0.0 26-27 0.0 0.0 0.0 0.0018283297525959958 0.0 28-29 0.0 0.0 0.0 0.004493352781803718 0.0 30-31 0.0 0.0 0.0 0.013851922023905255 0.0 32-33 0.0 0.0 0.0 0.03304938442616322 0.0 34-35 0.0 0.0 0.0 0.0606602625543502 0.0 36-37 0.0 0.0 0.0 0.10816584748197158 0.0 38-39 0.0 0.0 0.0 0.1805243215889487 0.0 40-41 0.0 0.0 0.0 0.2599946885471255 0.0 42-43 0.0 0.0 0.0 0.3416342602965179 0.0 44-45 0.0 0.0 0.0 0.4464998176318543 0.0 46-47 0.0 0.0 0.0 0.5684091268981704 0.0 48-49 0.0 0.0 0.0 0.6969964880574431 0.0 50-51 0.0 0.0 0.0 0.8398231292390522 0.0 52-53 0.0 0.0 0.0 0.9783888324379166 0.0 54-55 0.0 0.0 0.0 1.130310639422694 0.0 56-57 0.0 0.0 0.0 1.309254540378043 0.0 58-59 0.0 0.0 0.0 1.5052576875843084 0.0 60-61 0.0 0.0 0.0 1.7179327230430597 0.0 62-63 0.0 0.0 0.0 1.926966593316556 0.0 64-65 0.0 0.0 0.0 2.148039550181299 0.0 66-67 0.0 1.54943199372542E-5 0.0 2.4059734941767696 0.0 68-69 0.0 3.09886398745084E-5 0.0 2.691626776539988 0.0 70-71 0.0 3.09886398745084E-5 0.0 2.9920306514834727 0.0 72-73 0.0 3.09886398745084E-5 0.0 3.308424664602203 0.0 74-75 0.0 3.09886398745084E-5 0.0 3.631868593292385 0.0 76-77 0.0 3.09886398745084E-5 0.0 3.9876646620115532 0.0 78-79 0.0 3.09886398745084E-5 0.0 4.379516013224713 0.0 80-81 0.0 3.09886398745084E-5 0.0 4.789495718764458 0.0 82-83 0.0 4.64829598117626E-5 0.0 5.218920795825458 0.0 84-85 0.0 6.19772797490168E-5 0.0 5.663328880265784 0.0 86-87 0.0 6.19772797490168E-5 0.0 6.117327948747269 0.0 88-89 0.0 6.19772797490168E-5 0.0 6.592523246902918 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7015 0.0 42.20789 1 GTATCAA 11625 0.0 38.26627 1 ATCAACG 14585 0.0 30.157972 3 TATCAAC 14880 0.0 29.671947 2 TCAACGC 14840 0.0 29.671766 4 CAACGCA 15185 0.0 28.96635 5 AACGCAG 15685 0.0 28.091347 6 ACGCAGA 17885 0.0 24.635885 7 CGCAGAG 18065 0.0 24.337845 8 TACATGG 11930 0.0 23.10579 2 GTACATG 12225 0.0 23.053617 1 ACATGGG 11870 0.0 21.929434 3 GCAGAGT 19910 0.0 21.891754 9 TAAGGTG 1965 0.0 21.755922 5 GAGTACT 11160 0.0 21.22978 12-13 AGTACTT 11875 0.0 20.011496 12-13 AGAGTAC 17415 0.0 19.875275 10-11 GTACTTT 11705 0.0 19.855936 14-15 CAGAGTA 18770 0.0 19.186707 10-11 GTATAGG 870 0.0 19.119743 1 >>END_MODULE