FastQCFastQC Report
Thu 26 May 2016
SRR938434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3218644
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103270.320849401176396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75550.2347261766135056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56480.17547762349610582No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46320.14391153541677798No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA42970.13350342566621223No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42580.1322917352773404No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA39470.12262928115069577No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35560.11048130827764736No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35560.11048130827764736No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG33840.10513744297287926No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65900.045.0673521
GTATCAA112050.039.121061
TATCAAC142350.030.6468432
ATCAACG142300.030.4906313
TCAACGC144050.030.0542344
CAACGCA147900.029.2709795
AACGCAG154750.028.153866
ACGCAGA173350.024.9106627
CGCAGAG174300.024.7207648
TACATGG121050.023.8300232
GTACATG125050.023.6738851
TAAGGTG18750.023.5704965
ACATGGG121100.022.8783653
GAGTACT101500.022.11212212-13
GCAGAGT191100.021.9008269
GTGTAAG17900.021.8033711
GTACTTT106850.020.67283414-15
AGAGTAC163950.020.57210410-11
GTAAGGT20200.020.232414
AGTACTT107250.019.93011712-13
Produced by FastQC (version 0.11.5)